Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.12.2 - mitogen-activated protein kinase kinase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P32491

for references in articles please use BRENDA:EC2.7.12.2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
This enzyme is a dual-specific protein kinase and requires mitogen-activated protein kinase kinase kinase (MAPKKK) for activation. It is required for activation of EC 2.7.11.24, mitogen-activated protein kinase. Phosphorylation of MEK1 by Raf involves phosphorylation of two serine residues . Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumour necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischaemic injury.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P32491
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
mek/erk, mitogen-activated protein kinase kinase, map kinase kinase, mapkk, mek-1, extracellular-signal-regulated kinase, mapk/erk kinase, map2k4, erk 1, mitogen-activated protein kinase kinase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MAP kinase kinase MKK2/SSP33
-
MAP kinase kinase MKK1/SSP32
-
polymyxin B resistance protein kinase
-
serine/threonine protein kinase STE7
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (MAPKKK-activated)
This enzyme is a dual-specific protein kinase and requires mitogen-activated protein kinase kinase kinase (MAPKKK) for activation. It is required for activation of EC 2.7.11.24, mitogen-activated protein kinase. Phosphorylation of MEK1 by Raf involves phosphorylation of two serine residues [5]. Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumour necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischaemic injury.
CAS REGISTRY NUMBER
COMMENTARY hide
142805-58-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + protein
ADP + phosphoprotein
show the reaction diagram
MKK1 and MKK2 function in a signal transduction pathway involving the protein kinases encoded by PKC1, BCK1, and MPK1. The site of action for MKK1 and MKK2 is between BCK1 and MPK1
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + poly-(Tyr-Glu)
ADP + phospho-poly-(Tyr-Glu)
show the reaction diagram
-
tyrosine kinase substrate
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
ATP + Red1
ADP + phospho-Red1
show the reaction diagram
-
substrate of Mek1
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + protein
ADP + phosphoprotein
show the reaction diagram
MKK1 and MKK2 function in a signal transduction pathway involving the protein kinases encoded by PKC1, BCK1, and MPK1. The site of action for MKK1 and MKK2 is between BCK1 and MPK1
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
77500
x * 77500, calculation from nucleotide sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 77500, calculation from nucleotide sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
phylogenetic tree of kinases derived from the kinase core sequence, overview, expression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boyer, J.; Michaux, G.; Fairhead, C.; Gaillon, L.; Dujon, B.
Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae
Yeast
12
1575-1586
1996
Saccharomyces cerevisiae (P32490)
Manually annotated by BRENDA team
Bussey, H.; Storms, R.K.; Ahmed, A.; Albermann, K.; et al.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI
Nature
387
103-105
1997
Saccharomyces cerevisiae (P32491)
Manually annotated by BRENDA team
Brewster, J.L.; de Valoir, T.; Dwyer, N.D.; Winter, E.; Gustin, M.C.
An osmosensing signal transduction pathway in yeast
Science
259
1760-1763
1993
Saccharomyces cerevisiae (P08018), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Cairns, B.R.; Ramer, S.W.; Kornberg, R.D.
Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase
Genes Dev.
6
1305-1318
1992
Saccharomyces cerevisiae (P06784)
Manually annotated by BRENDA team
Delaveau, T.; Blugeon, C.; Jacq, C.; Perea, J.
Analysis of a 23 kb region on the left arm of yeast chromosome IV
Yeast
12
1587-1592
1996
Saccharomyces cerevisiae (P06784)
Manually annotated by BRENDA team
Teague, M.A.; Chaleff, D.T.; Errede, B.
Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases
Proc. Natl. Acad. Sci. USA
83
7371-7375
1986
Saccharomyces cerevisiae (P06784)
Manually annotated by BRENDA team
Zheng, C.F.; Guan, K.L.
Activation of MEK family kinases requires phosphorylation of two conserved Ser/Thr residues
EMBO J.
13
1123-1131
1994
Saccharomyces cerevisiae (P06784), Homo sapiens (Q02750)
Manually annotated by BRENDA team
Boguslawski, G.; Polazzi, J.O.
Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases
Proc. Natl. Acad. Sci. USA
84
5848-5852
1987
Saccharomyces cerevisiae (P08018)
Manually annotated by BRENDA team
Cziepluch, C.; Kordes, E.; Pujol, A.; Jauniaux, J.C.
Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon
Yeast
12
1471-1474
1996
Saccharomyces cerevisiae (P08018)
Manually annotated by BRENDA team
Maeda, T.; Takekawa, M.; Saito, H.
Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor
Science
269
554-558
1995
Saccharomyces cerevisiae (P08018), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Irie, K.; Takase, M.; Lee, K.S.; Levin, D.E.; Araki, H.; Matsumoto, K.; Oshima, Y.
MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C
Mol. Cell. Biol.
13
3076-3083
1993
Saccharomyces cerevisiae (P32490), Saccharomyces cerevisiae (P32491)
Manually annotated by BRENDA team
Zhu, H.; Klemic, J.F.; Chang, S.; Bertone, P.; Casamayor, A.; Klemic, K.G.; Smith, D.; Gerstein, M.; Reed, M.A.; Snyder, M.
Analysis of yeast protein kinases using protein chips
Nat. Genet.
26
283-289
2000
Saccharomyces cerevisiae
Manually annotated by BRENDA team