Any feedback?
Information on EC 2.7.12.1 - dual-specificity kinase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P22216
for references in articles please use BRENDA:EC2.7.12.1Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.7.12.1 dual-specificity kinaseIUBMB Comments
This family of enzymes can phosphorylate both Ser/Thr and Tyr residues.
Specify your search results
Word Map
- 2.7.12.1
- alzheimer
-
dyrks
- autophosphorylation
- tau
- deficit
-
intellectual
- disability
- harmine
- neurodegenerative
-
neurogenesis
- microcephaly
-
trisomic
-
neurodevelopmental
-
ts65dn
- nfat
-
tyrosine-phosphorylated
-
neurofibrillary
-
hyperphosphorylation
-
autism
-
haploinsufficiency
-
tangle
-
cdc2-like
- biliverdin
-
kinome
- ck2
-
proline-directed
- medicine
-
euploid
- gli1
-
atp-competitive
- rcan1
- dscr1
- analysis
- diagnostics
- drug development
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
dyrk1a, dyrk2, dyrk1b, dyrk3, dual-specificity kinase, dyrk1, dual specificity kinase, dual-specificity tyrosine phosphorylation-regulated kinase 1a, dual-specificity protein kinase, dyrk1a kinase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
protein kinase SPK1
-
Clik1
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (Ser/Thr- and Tyr-phosphorylating)
This family of enzymes can phosphorylate both Ser/Thr and Tyr residues.
CAS REGISTRY NUMBER
COMMENTARY
134549-83-0
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
phosphorylation on a C-terminal Ser residue
-
-
?
ATP + poly-(Tyr-Glu)
ADP + phospho-poly-(Tyr-Glu)
-
tyrosine kinase substrate
-
-
?
ATP + protein
ADP + phosphoprotein
the enzyme phosphorylates proteins on serine, threonine, and tyrosine
-
-
?
ATP + protein
ADP + phosphoprotein
Rad53 autophosphorylation activity depends on trans phosphorylation mediated by Mec1 and does not require physical association with other proteins
-
-
?
ATP + protein
ADP + phosphoprotein
autophosphorylation on Tyr residues
-
-
?
ATP + protein
ADP + phosphoprotein
kinase can phosphorylate serine, threonine and tyrosine residues
-
-
?
ATP + SR protein
ADP + ?
functions as a negative regulator of the cell cycle in Saccharomyces cerevisiae, acting downstream of the cAMP-dependent protein kinase
-
-
?
ATP + SR protein
ADP + ?
Yak1 acts downstream from, or on a parallel pathway to, the kinase step in the Ras/cAMP pathway
-
-
?
ATP + SR protein
ADP + ?
Yak1p and Pop2p are part of a novel glucose-sensing system in yeast that is involved in growth control in response to glucose availability
-
-
?
additional information
?
-
required for the execution of checkpoint arrest at multiple stages of the cell cycle. Rad53 modulates the lagging strand replication apparatus by controlling phosphorylation of the DNA polymerase alpha-primase complex in response to intra-S DNA damage
-
-
?
additional information
?
-
-
required for the execution of checkpoint arrest at multiple stages of the cell cycle. Rad53 modulates the lagging strand replication apparatus by controlling phosphorylation of the DNA polymerase alpha-primase complex in response to intra-S DNA damage
-
-
?
additional information
?
-
Rad53 exerts its role in checkpoint control through regulation of the Polo kinase Cdc5
-
-
?
additional information
?
-
the SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast
-
-
?
additional information
?
-
the enzyme is not essential for cell growth and a variety of other cellular processes in yeast
-
-
?
additional information
?
-
negative regulation of filamentous growth and flocculation
-
-
?
additional information
?
-
-
negative regulation of filamentous growth and flocculation
-
-
?
additional information
?
-
may function in a checkpoint control which couples DNA replication to mitosis. The level of the RPK1 transcript is extremely low and constant throughout the mitotic cycle. However it is regulated during cellular differentiation, being decreased in alpha-factor-treated cells and increased late in meiosis in alpha diploids. Rpk1 is involved in a pathway that coordinates cell proliferation and differentiation
-
-
?
additional information
?
-
-
may function in a checkpoint control which couples DNA replication to mitosis. The level of the RPK1 transcript is extremely low and constant throughout the mitotic cycle. However it is regulated during cellular differentiation, being decreased in alpha-factor-treated cells and increased late in meiosis in alpha diploids. Rpk1 is involved in a pathway that coordinates cell proliferation and differentiation
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + SR protein
ADP + ?
functions as a negative regulator of the cell cycle in Saccharomyces cerevisiae, acting downstream of the cAMP-dependent protein kinase
-
-
?
ATP + SR protein
ADP + ?
Yak1 acts downstream from, or on a parallel pathway to, the kinase step in the Ras/cAMP pathway
-
-
?
ATP + SR protein
ADP + ?
Yak1p and Pop2p are part of a novel glucose-sensing system in yeast that is involved in growth control in response to glucose availability
-
-
?
additional information
?
-
required for the execution of checkpoint arrest at multiple stages of the cell cycle. Rad53 modulates the lagging strand replication apparatus by controlling phosphorylation of the DNA polymerase alpha-primase complex in response to intra-S DNA damage
-
-
?
additional information
?
-
-
required for the execution of checkpoint arrest at multiple stages of the cell cycle. Rad53 modulates the lagging strand replication apparatus by controlling phosphorylation of the DNA polymerase alpha-primase complex in response to intra-S DNA damage
-
-
?
additional information
?
-
Rad53 exerts its role in checkpoint control through regulation of the Polo kinase Cdc5
-
-
?
additional information
?
-
the SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast
-
-
?
additional information
?
-
the enzyme is not essential for cell growth and a variety of other cellular processes in yeast
-
-
?
additional information
?
-
negative regulation of filamentous growth and flocculation
-
-
?
additional information
?
-
-
negative regulation of filamentous growth and flocculation
-
-
?
additional information
?
-
may function in a checkpoint control which couples DNA replication to mitosis. The level of the RPK1 transcript is extremely low and constant throughout the mitotic cycle. However it is regulated during cellular differentiation, being decreased in alpha-factor-treated cells and increased late in meiosis in alpha diploids. Rpk1 is involved in a pathway that coordinates cell proliferation and differentiation
-
-
?
additional information
?
-
-
may function in a checkpoint control which couples DNA replication to mitosis. The level of the RPK1 transcript is extremely low and constant throughout the mitotic cycle. However it is regulated during cellular differentiation, being decreased in alpha-factor-treated cells and increased late in meiosis in alpha diploids. Rpk1 is involved in a pathway that coordinates cell proliferation and differentiation
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
activation of Rad53 in response to DNA damage in G(1) requires the Rad9, Mec3, Ddc1, Rad17 and Rad24 checkpoint factors, while this dependence is greatly reduced in S phase cells. Furthermore, during recovery from checkpoint activation, Rad53 activity decreases through a process that does not require protein synthesis
-
additional information
-
activation of Rad53 in response to DNA damage in G(1) requires the Rad9, Mec3, Ddc1, Rad17 and Rad24 checkpoint factors, while this dependence is greatly reduced in S phase cells. Furthermore, during recovery from checkpoint activation, Rad53 activity decreases through a process that does not require protein synthesis
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
phylogenetic tree of kinases derived from the kinase core sequence, overview, overexpression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sanchez, Y.; Bachant, J.; Wang, H.; Hu, F.; Liu, D.; Tetzlaff, M.; Elledge, S.J.
Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms
Science
286
1166-1171
1999
Saccharomyces cerevisiae (P22216)
Pellicioli, A.; Lucca, C.; Liberi, G.; et al.
Activation of Rad53 kinase in response to DNA damage and its effect in modulating phosphorylation of the lagging strand DNA polymerase
EMBO J.
18
6561-6572
1999
Saccharomyces cerevisiae (P22216), Saccharomyces cerevisiae
Liao, H.; Byeon, I.J.; Tsai, M.D.
Structure and function of a new phosphopeptide-binding domain containing the FHA2 of Rad53
J. Mol. Biol.
294
1041-1049
1999
Saccharomyces cerevisiae (P22216)
Purnelle, B.; Coster, F.; Goffeau, A.
The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators
Yeast
12
1483-1492
1996
Saccharomyces cerevisiae (P22216)
Allen, J.B.; Zhou, Z.; Siede, W.; Friedberg, E.C.; Elledge, S.J.
The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast
Genes Dev.
8
2401-2415
1994
Saccharomyces cerevisiae (P22216)
Stern, D.F.; Zheng, P.; Beidler, D.R.; Zerillo, C.
Spk1, a new kinase from Saccharomyces cerevisiae, phosphorylates proteins on serine, threonine, and tyrosine
Mol. Cell. Biol.
11
987-1001
1991
Saccharomyces cerevisiae (P22216)
Bussey, H.; Storms, R.K.; Ahmed, A.; Albermann, K.; et al.
The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI
Nature
387
103-105
1997
Saccharomyces cerevisiae (P22216)
Kim, K.H.; Cho, Y.M.; Kang, W.H.; Kim, J.H.; Byun, K.H.; Park, Y.D.; Bae, K.S.; Park, H.M.
Negative regulation of filamentous growth and flocculation by Lkh1, a fission yeast LAMMER kinase homolog
Biochem. Biophys. Res. Commun.
289
1237-1242
2001
Saccharomyces cerevisiae (P32350), Saccharomyces cerevisiae, Schizosaccharomyces pombe (Q10156), Schizosaccharomyces pombe
Purnelle, B.; Goffeau, A.
The sequence of 32b on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABS transporter homologous to the human multidrug resistance protein
Yeast
13
183-188
1997
Saccharomyces cerevisiae (P32350)
Lee, K.; Du, C.; Horn, M.; Rabinow, L.
Activity and autophosphorylation of LAMMER protein kinases
J. Biol. Chem.
271
27299-27303
1996
Saccharomyces cerevisiae (P32350), Homo sapiens (P49760), Drosophila melanogaster (P49762)
Padmanabha, R.; Gehrung, S.; Snyder, M.
The KNS1 gene of Saccharomyces cerevisiae encodes a nonessential protein kinase homologue that is distantly related to members of the CDC28/cdc2 gene family
Mol. Gen. Genet.
229
1-9
1991
Saccharomyces cerevisiae (P32350)
Moriya, H.; Shimizu-Yoshida, Y.; Omori, A.; Iwashita, S.; Katoh, M.; Sakai, A.
Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal
Genes Dev.
15
1217-1228
2001
Saccharomyces cerevisiae (P14680)
Kassis, S.; Melhuish, T.; Annan, R.S.; Chen, S.L.; Lee, J.C.; Livi, G.P.; Creasy, C.L.
Saccharomyces cerevisiae Yak1p protein kinase autophosphorylates on tyrosine residues and phosphorylates myelin basic protein on a C-terminal serine residue
Biochem. J.
348
263-272
2000
Saccharomyces cerevisiae (P14680)
-
Katsoulou, C.; Tzermia, M.; Tavernarakis, N.; Alexandraki, D.
Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI
Yeast
12
787-797
1996
Saccharomyces cerevisiae (P14680)
Garrett, S.; Broach, J.
Loss of Ras activity in Saccharomyces cerevisiae is suppressed by disruptions of a new kinase gene, YAKI, whose product may act downstream of the cAMP-dependent protein kinase
Genes Dev.
3
1336-1348
1989
Saccharomyces cerevisiae (P14680)
Saren, A.M.; Laamanen, P.; Lejarcegui, J.B.; Paulin, L.
The sequence of a 36.7 kb segment on the left arm of chromosome IV from Saccharomyces cerevisiae reveals 20 non-overlapping open reading frames (ORFs) including SIT4, FAD1, NAM1, RNA11, SIR2, NAT1, PRP9, ACT2 and MPS1 and 11 new ORFs
Yeast
13
65-71
1997
Saccharomyces cerevisiae (P54199)
Lauze, E.; Stoelcker, B.; Luca, F.C.; Weiss, E.; Schutz, A.R.; Winey, M.
Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase
EMBO J.
14
1655-1663
1995
Saccharomyces cerevisiae (P54199), Saccharomyces cerevisiae
Poch, O.; Schwob, E.; de Fraipont, F.; Camasses, A.; Bordonne, R.; Martin, R.P.
RPK1, an essential yeast protein kinase involved in the regulation of the onset of mitosis, shows homology to mammalian dual-specificity kinases
Mol. Gen. Genet.
243
641-653
1994
Saccharomyces cerevisiae (P54199), Saccharomyces cerevisiae
EXTERNAL LINKS (specific for EC-Number 2.7.12.1)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
