Information on EC 2.7.11.28 - tropomyosin kinase

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The expected taxonomic range for this enzyme is: Amniota

EC NUMBER
COMMENTARY
2.7.11.28
-
RECOMMENDED NAME
GeneOntology No.
tropomyosin kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + tropomyosin = ADP + O-phosphotropomyosin
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:tropomyosin O-phosphotransferase
The enzyme phosphorylates casein equally well, and histone and phosvitin to a lesser extent. The acceptor is a serine residue in the protein.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
tropomyosin kinase (phosphorylating)
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
90804-56-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alphaalpha-tropomyosin + ATP
?
show the reaction diagram
-
-
-
-
?
ATP + alpha-tropomyosin
ADP + O-phosphotropomyosin
show the reaction diagram
-
involved in phosphorylation of embryonal skeletal muscle proteins
-
-
?
ATP + casein
ADP + phosphocasein
show the reaction diagram
-
-
-
-
?
ATP + histone II-A
ADP + phosphohistone II-A
show the reaction diagram
-
-
-
-
?
ATP + KLKYKAISEELDHALNDITS(P)L
?
show the reaction diagram
-
peptide beta[264-284]
-
-
?
ATP + KLKYKAISEELDHALNDMTS(P)I
?
show the reaction diagram
-
peptide alpha[264-284]
-
-
?
ATP + KLKYKAISEELDNALNDITS(P)I
?
show the reaction diagram
-
peptide Ile-284-beta[264-284]
-
-
?
ATP + KLKYKAISEELDNALNDITS(P)L
?
show the reaction diagram
-
peptide His-276-beta[264-284]
-
-
?
ATP + KLKYKAISEELDNALNDMTS(P)L
?
show the reaction diagram
-
peptide Met-281-beta[264-284]
-
-
?
ATP + phosphorylase b
ADP + phosphophosphorylase b
show the reaction diagram
-
-
-
-
?
ATP + phosvitin
ADP + phosphophosvitin
show the reaction diagram
-
-
-
-
?
ATP + tropomyosin
ADP + O-phosphotropomyosin
show the reaction diagram
-
-
-
-
?
ATP + tropomyosin
ADP + O-phosphotropomyosin
show the reaction diagram
-
beta-tropomyosin from turkey gizzard
-
-
?
ATP + tropomyosin
ADP + O-phosphotropomyosin
show the reaction diagram
-
other poor substrates are beta-tropomyosin from chicken leg muscle, rabbit skeletal muscle, the phosphorylation site is a single serine-residue close to COOH-terminus, i.e Ser-283
-
?
ATP + tropomyosin
ADP + O-phosphotropomyosin
show the reaction diagram
-
alpha-tropomyosin subunit preferred over beta-tropomyosin subunit
-
-
?
ATP + tropomyosin
ADP + O-phosphotropomyosin
show the reaction diagram
-
alpha-tropomyosin subunit preferred over beta-tropomyosin subunit
-
?
ATP + troponin complex
ADP + phosphotroponin complex
show the reaction diagram
-
from rabbit skeletal muscle
-
-
?
betabeta-tropomyosin + ATP
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no substrates are smooth muscle myosin light chain from turkey gizzard, tropomyosin from platelet and erythrocyte from chicken gizzard and smooth muscle
-
-
-
additional information
?
-
-
no substrates are gamma- or beta-tropomyosin from smooth muscle from chicken gizzard
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + alpha-tropomyosin
ADP + O-phosphotropomyosin
show the reaction diagram
-
involved in phosphorylation of embryonal skeletal muscle proteins
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
no activation by cAMP or calmodulin
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
requirement
additional information
-
no activation by Ca2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
KCl
-
high concentrations
additional information
-
no inhibition by heparin
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
DTT
-
requirement
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.05
-
alpha-tropomyosin
-
37C, pH 7.5, skeletal alpha-tropomyosin
0.047
-
alphaalpha-tropomyosin
-
37C, pH 7.5
-
0.5
-
alpha[264-284]
-
37C, pH 7.5
-
0.2
-
ATP
-
37C, pH 7.5
0.264
-
betabeta-tropomyosin
-
37C, pH 7.5
-
0.66
-
beta[264-284]
-
37C, pH 7.5
-
1.48
-
His-276-beta[264-284]
-
37C, pH 7.5
-
1.36
-
Ile-284-beta[264-284]
-
37C, pH 7.5
-
0.33
-
Met-281-beta[264-284]
-
37C, pH 7.5
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0004
-
-
-
0.0594
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.2
9.5
-
about half-maximal activity at pH 6.2 and 9.5
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
about
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
40
-
about 10% of maximal activity at 22C and about 60% of maximal activity at 40C
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
additional information
-
not in adult chicken or adult rabbit skeletal muscle
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
252000
-
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
highly purified enzyme is very unstable upon further purification
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, in 10% sucrose at least 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE