Information on EC 2.7.11.27 - [acetyl-CoA carboxylase] kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.11.27
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RECOMMENDED NAME
GeneOntology No.
[acetyl-CoA carboxylase] kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + [acetyl-CoA carboxylase] = ADP + [acetyl-CoA carboxylase] phosphate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:[acetyl-CoA carboxylase] phosphotransferase
Phosphorylates and inactivates EC 6.4.1.2, acetyl-CoA carboxylase, which can be dephosphorylated and reactivated by EC 3.1.3.17, [phosphorylase] phosphatase. The enzyme is more active towards the dimeric form of acetyl-CoA carboxylase than the polymeric form [5]. Phosphorylates serine residues.
CAS REGISTRY NUMBER
COMMENTARY hide
77000-06-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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AMP-activated protein kinase (AMPK) is an evolutionarily conserved serine/threonine protein kinase
malfunction
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phosphorylated enzyme AMPK is involved in impaired glucose metabolism, that increases the risk for squamous cell carcinoma of the head and neck (SCCHN) in humans
metabolism
physiological function
additional information
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AMPK is activated in response to metabolic stresses, such as hypoxia and ischemia, and the anti-diabetic drug metformin
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + ATP-citrate lyase
ADP + (ATP-citrate lyase) phosphate
show the reaction diagram
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-
-
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?
ATP + casein
ADP + phosphocasein
show the reaction diagram
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-
-
-
?
ATP + glycogen synthase
ADP + (glycogen synthase) phosphate
show the reaction diagram
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-
-
-
?
ATP + histone
ADP + phosphohistone
show the reaction diagram
ATP + phosphorylase kinase
ADP + (phosphorylase kinase) phosphate
show the reaction diagram
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poor substrate
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-
?
ATP + phosvitin
ADP + phosvitin phosphate
show the reaction diagram
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-
-
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?
ATP + protamine
ADP + protamine phosphate
show the reaction diagram
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-
-
-
?
ATP + pyruvate kinase L
ADP + (pyruvate kinase L) phosphate
show the reaction diagram
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very poor substrate
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-
?
ATP + [acetyl-CoA carboxylase]
ADP + [acetyl-CoA carboxylase] phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + [acetyl-CoA carboxylase]
ADP + [acetyl-CoA carboxylase] phosphate
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-D-glucose 6-phosphate
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alpha-glycerophosphate
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AMP
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7% inhibition at 0.8 mM
ARA-A
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a competitive inhibitor of AMPK
beta-Glycerophosphate
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citrate
CoA
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16% inhibition at 0.1 mM
Insulin
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-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adiponectin
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adiponectin increases the phosphorylation and activation of AMPK, it increases inactivated acetyl-CoA carboxylase (ACC), which has been phosphorylated by activated AMPK, and subsequently increases expression of agouti-related peptide (AgRP) mRNA
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cAMP-dependent protein kinase
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phosphorylates and activates carboxylase kinase
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CTRP1 protein
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activates AMP-activated protein kinase (AMPK), which inhibits acetyl-CoA carboxylase (ACC) through phosphorylation. CTRP1 has a direct effect on AMPK signaling, recombinant CTRP1 administration acutely stimulates muscle AMPKalpha and ACC phosphorylation in vivo
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Deamino-CoA
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dephospho-CoA
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leptin
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stimulates fatty acid oxidation via the phosphorylation of AMPK and acetyl-CoA carboxylase, the stimulation by leptin is muscle fibre type-dependent and fails in diet-induced obesity
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metformin
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an AMPK activator
Oxidized CoA
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additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000045 - 0.0003
acetyl-CoA carboxylase
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0.02 - 0.03
ATP
0.005
histone
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0.0008
protamine
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
30
alpha-D-glucose 6-phosphate
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20
alpha-glycerophosphate
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10
beta-Glycerophosphate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.008
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purified enzyme
0.5
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purified enzyme
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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a mouse immortalized hypothalamic neuronal cell line
Manually annotated by BRENDA team
additional information
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phosphorylated AMPK and phosphorylated acetyl-CoA carboxylase are mainly found in primary human tumor specimens
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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in primary human tumor specimens
Manually annotated by BRENDA team
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in healthy mucosa tissue
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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SDS-PAGE
53000
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SDS-PAGE, two bands
65000
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SDS-PAGE, two bands
90000
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SDS-PAGE, two lighter bands at 40000 and 60000 Da detected
105000
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gel filtration ACK3
130000
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gel filtration
160000 - 200000
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gel filtration
170000
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SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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high molecular weight aggregates, gel filtration
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10 mM phosphate buffer, pH 7, 2 mM EDTA, 10 mM 2-mercaptoethanol, 20% glycerol, 4 weeks, no loss of activity
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-70°C, partially purified kinase preparation, stable for several months, highly purified kinase is very labile and loses activity within 5 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial purification of ACK2 and ACK3
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
recombinant CTRP1 acutely induces AMPKalpha and ACC phosphorylation in skeletal muscle
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
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strong expression of phosphorylated acetyl-CoA carboxylase is an independent prognostic marker for patients with nodepositive of squamous cell carcinoma of the head and neck
medicine