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Information on EC 2.7.11.24 - mitogen-activated protein kinase and Organism(s) Nicotiana tabacum and UniProt Accession Q40531

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EC Tree
IUBMB Comments
Phosphorylation of specific tyrosine and threonine residues in the activation loop of this enzyme by EC 2.7.12.2, mitogen-activated protein kinase kinase (MAPKK) is necessary for enzyme activation. Once activated, the enzyme phosphorylates target substrates on serine or threonine residues followed by a proline . A distinguishing feature of all MAPKs is the conserved sequence Thr-Xaa-Tyr (TXY). Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumour necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischaemic injury.
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Nicotiana tabacum
UNIPROT: Q40531
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The taxonomic range for the selected organisms is: Nicotiana tabacum
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
hide
ATP
+
a [protein]-(L-serine/L-threonine)
=
ADP
+
a [protein]-(L-serine/L-threonine) phosphate
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
mapk, p38, erk1/2, p38 mapk, mitogen-activated protein kinase, map kinase, extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, p38mapk, p38 map kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mitogen-activated protein kinase homolog NTF6
-
cp38a
-
-
-
-
cp38b
-
-
-
-
CSAID binding protein
-
-
-
-
CSBP
-
-
-
-
Cytokine suppressive anti-inflammatory drug binding protein
-
-
-
-
ERK5
-
-
-
-
MAP kinase MXI2
-
-
-
-
MAP kinase p38 beta
-
-
-
-
MAP kinase p38 delta
-
-
-
-
MAP kinase p38 gamma
-
-
-
-
MAP kinase p38a
-
-
-
-
MAP kinase p38alpha
-
-
-
-
MAP kinase p38b
-
-
-
-
mitogen-activated protein kinase
-
-
-
-
mitogen-activated protein kinase homolog NTF3
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mitogen-activated protein kinase homolog NTF4
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Mitogen-activated protein kinase p38 beta
-
-
-
-
Mitogen-activated protein kinase p38 delta
-
-
-
-
Mitogen-activated protein kinase p38 gamma
-
-
-
-
Mitogen-activated protein kinase p38a
-
-
-
-
Mitogen-activated protein kinase p38alpha
-
-
-
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Mitogen-activated protein kinase p38b
-
-
-
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p38b
-
-
-
-
salicylic acid-induced protein kinase
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SAPK2A
-
-
-
-
stress-activated protein kinase 2a
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (MAPKK-activated)
Phosphorylation of specific tyrosine and threonine residues in the activation loop of this enzyme by EC 2.7.12.2, mitogen-activated protein kinase kinase (MAPKK) is necessary for enzyme activation. Once activated, the enzyme phosphorylates target substrates on serine or threonine residues followed by a proline [6]. A distinguishing feature of all MAPKs is the conserved sequence Thr-Xaa-Tyr (TXY). Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumour necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischaemic injury.
CAS REGISTRY NUMBER
COMMENTARY hide
142243-02-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
show the reaction diagram
-
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
autophosphorylation
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
show the reaction diagram
-
-
-
?
ATP + nodulation outer protein L
ADP + a phosphorylated nodulation outer protein L
show the reaction diagram
nodulation outer protein L variants with six or eight serine to alanine substitutions are partially active, whereas nodulation outer protein L forms with 10 or 12 substituted serine residues are inactive
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
autophosphorylation
-
-
?
ATP + WRKY1
ADP + phosphorylated WRKY1
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + nodulation outer protein L
ADP + a phosphorylated nodulation outer protein L
show the reaction diagram
nodulation outer protein L variants with six or eight serine to alanine substitutions are partially active, whereas nodulation outer protein L forms with 10 or 12 substituted serine residues are inactive
-
-
?
ATP + WRKY1
ADP + phosphorylated WRKY1
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
supports reaction with myelin basic protein
Mn2+
supports phosphorylation of myelin basic protein more strongly than Mg2+
Mg2+
supports reaction with myelin basic protein
Mn2+
supports phosphorylation of myelin basic protein more strongly than Mg2+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NTF6_TOBAC
371
0
42742
Swiss-Prot
other Location (Reliability: 1)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli as a fusion protein with glutathione-S-transferase
expression in Escherichia coli as a fusion protein with glutathione-S-transferase
GST- or Hos6-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wilson, C.; Anglmayer, R.; Vicente, O.; Heberle-Bors, E.
Molecular cloning, functional expression in Escherichia coli, and characterization of multiple mitogen-activated-protein kinases from tobacco
Eur. J. Biochem.
233
249-257
1995
Nicotiana tabacum (Q40517), Nicotiana tabacum (Q40531), Nicotiana tabacum (Q40532)
Manually annotated by BRENDA team
Wilson, C.; Eller, N.; Gartner, A.; Vicente, O.; Heberle-Bors, E.
Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase
Plant Mol. Biol.
23
543-551
1993
Nicotiana tabacum (Q40517), Nicotiana tabacum
Manually annotated by BRENDA team
Ge, Y.; Xiang, Q.; Wagner, C.; Zhang, D.; Xie, Z.; Staehelin, C.
The type 3 effector NopL of Sinorhizobium sp. strain NGR234 is a mitogen-activated protein kinase substrate
J. Exp. Bot.
67
2483-2494
2016
Nicotiana tabacum (O04362)
Manually annotated by BRENDA team