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Information on EC 2.7.11.24 - mitogen-activated protein kinase and Organism(s) Arabidopsis thaliana and UniProt Accession Q39026
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2.7.11.24 mitogen-activated protein kinaseIUBMB Comments
Phosphorylation of specific tyrosine and threonine residues in the activation loop of this enzyme by EC 2.7.12.2, mitogen-activated protein kinase kinase (MAPKK) is necessary for enzyme activation. Once activated, the enzyme phosphorylates target substrates on serine or threonine residues followed by a proline . A distinguishing feature of all MAPKs is the conserved sequence Thr-Xaa-Tyr (TXY). Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumour necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischaemic injury.
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Word Map
- 2.7.11.24
- endothelial
- necrosis
- tnf
- agonist
- lipopolysaccharide
- 3-kinase
- pkc
- lps
- nf-kappab
- sirnas
- phosphatidylinositol
- caspase-3
- cardiac
- metastasis
-
lps-induced
- artery
- camp
- bcl-2
- monocyte
- chemokine
- collagen
- angiogenesis
- cox-2
- fibrosis
-
toll-like
- cyclins
-
neuroprotective
- melanoma
- stat3
- tnf-alpha
- prostaglandin
- c-fos
- phosphoinositide
- mtor
- cardiomyocytes
- factor-alpha
- osteoblast
-
dominant-negative
- creb
-
anti-apoptotic
- integrins
- cyclooxygenase-2
- angiotensin
- wortmannin
-
protein-coupled
- microglia
- mmp-9
- il-1beta
-
platelet-derived
- fak
- medicine
- drug development
- analysis
- pharmacology
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
mapk, p38, erk1/2, p38 mapk, mitogen-activated protein kinase, map kinase, extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, p38mapk, p38 map kinase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
cp38a
-
-
-
-
cp38b
-
-
-
-
CSAID binding protein
-
-
-
-
CSBP
-
-
-
-
Cytokine suppressive anti-inflammatory drug binding protein
-
-
-
-
ERK5
-
-
-
-
MAP kinase MXI2
-
-
-
-
MAP kinase p38 beta
-
-
-
-
MAP kinase p38 delta
-
-
-
-
MAP kinase p38 gamma
-
-
-
-
MAP kinase p38a
-
-
-
-
MAP kinase p38alpha
-
-
-
-
MAP kinase p38b
-
-
-
-
MAPK
mitogen-activated protein kinase
Mitogen-activated protein kinase p38 beta
-
-
-
-
Mitogen-activated protein kinase p38 delta
-
-
-
-
Mitogen-activated protein kinase p38 gamma
-
-
-
-
Mitogen-activated protein kinase p38a
-
-
-
-
Mitogen-activated protein kinase p38alpha
-
-
-
-
Mitogen-activated protein kinase p38b
-
-
-
-
MPK4
p38b
-
-
-
-
SAPK2A
-
-
-
-
stress-activated protein kinase 2a
-
-
-
-
MAPK
-
-
-
-
mitogen-activated protein kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (MAPKK-activated)
Phosphorylation of specific tyrosine and threonine residues in the activation loop of this enzyme by EC 2.7.12.2, mitogen-activated protein kinase kinase (MAPKK) is necessary for enzyme activation. Once activated, the enzyme phosphorylates target substrates on serine or threonine residues followed by a proline [6]. A distinguishing feature of all MAPKs is the conserved sequence Thr-Xaa-Tyr (TXY). Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumour necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischaemic injury.
CAS REGISTRY NUMBER
COMMENTARY
142243-02-5
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + AGDSTPEELANATQVQGDYLPIVR
ADP + AGDSpTPEELANATQVQGDYLPIVR
-
-
-
?
ATP + Arabidopsis thaliana protein AT1G7815
ADP + phosphorylated Arabidopsis thaliana proteins AT1G78150
-
-
-
?
ATP + Arabidopsis thaliana protein AT2G26530
ADP + phosphorylated Arabidopsis thaliana proteins AT2G26530
-
-
-
?
ATP + Arabidopsis thaliana protein AT3G11330
ADP + phosphorylated Arabidopsis thaliana proteins AT3G11330
-
-
-
?
ATP + Arabidopsis thaliana protein AT4G38710
ADP + phosphorylated Arabidopsis thaliana proteins AT4G38710
-
-
-
?
ATP + ARF-GAP domain 9
ADP + phosphorylated ARF-GAP domain 9
-
-
-
?
ATP + DAETVNQTSHPTEEEAQVTVSSNADVEDSHETVSPR
ADP + DAETVNQTSHPTEEEAQVTVSSNADVEDSHETVpSPR
-
-
-
?
ATP + DIQGSDNAIPLSPQWLLSKPGENK
ADP + DIQGSDNAIPLpSPQWLLSKPGENK
-
-
-
?
ATP + FDWDHPLHLQPMSPTTVK
ADP + FDWDHPLHLQPMpSPTTVK
-
-
-
?
ATP + FTDSALASAVFSPTHK
ADP + FTDSALASAVFpSPTHK
-
-
-
?
ATP + GMVSSGGPVSPGPVYPGGRPGAGGLMPGMPGTR
ADP + GMVSSGGPVpSPGPVYPGGRPGAGGLMPGMPGTR
-
-
-
?
ATP + HAP5 cation-chloride co-transporter 1
ADP + phosphorylated HAP5 cation-chloride co-transporter 1
-
-
-
?
ATP + KFSEQNIGAPPSYEEAVSDSRSPVYSER
ADP + KFpSEQNIGAPPSYEEAVSDSRpSPVYSER
-
-
-
?
ATP + La protein 1
ADP + phosphorylated La protein 1
-
-
-
?
ATP + MAP65-1
ADP + phosphorylated MAP65-1
microtubule-associated protein, phosphorylation in vitro by MPK6, recombinant GST-tagged substrate protein
-
-
?
ATP + mitogen-activated protein kinase phosphatase 1
ADP + phosphorylated mitogen-activated protein kinase phosphatase 1
-
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
-
-
-
?
ATP + NLSLNPTASAAPVTPPKKDDKPEDILFK
ADP + NLSLNPTASAAPVpTPPKKDDKPEDILFK
-
-
-
?
ATP + NVEKVEEIRSPQTINK
ADP + NVEKVEEIRpSPQTINK
-
-
-
?
ATP + phosphoenolpyruvate carboxykinase 1
ADP + phosphorylated phosphoenolpyruvate carboxykinase 1
-
-
-
?
ATP + SKDSNVTPDDDVSGMRSPSAFFK
ADP + SKDSNVTPDDDVSGMRpSPSAFFK
-
-
-
?
ATP + target of Myb protein 1
ADP + phosphorylated target of Myb protein 1
-
-
-
?
ATP + TEEGEMVNNNVSPMMHSR
ADP + TEEGEMVNNNVpSPMMHSR
-
-
-
?
ATP + TLSYPTPPLNLQSPR
ADP + TLSYPTPPLNLQpSPR
-
-
-
?
ATP + TVANSPEALQSPHSSESAFALK
ADP + TVANSPEALQpSPHSSESAFALK
-
-
-
?
ATP + VVEEKPASPEPVKAEAEKPVEK
ADP + VVEEKPApSPEPVKAEAEKPVEK
-
-
-
?
ATP + YEEMNKPSAPLTSHEPAMIPVAEEPDDSPIHGREESLVR
ADP + YEEMNKPSAPLTSHEPAMIPVAEEPDDpSPIHGREESLVR
-
-
-
?
ATP + YSLVLDPNLDAGTPR
ADP + YSLVLDPNLDAGpTPR
-
-
-
?
ATP + YVEEWVGPGSPMNSPR
ADP + YVEEWVGPGSPMNpSPR
-
-
-
?
ATPgammaS + myelin basic protein
ADP + thiophosphorylated myelin basic protein
Arabidopsis thaliana MPKs use ATPgammaS to thiophosphorylate myelin basic protein
-
-
?
ATP + Arabidopsis thaliana protein AT1G7815
ADP + phosphorylated Arabidopsis thaliana proteins AT1G78150
-
-
-
?
ATP + Arabidopsis thaliana protein AT2G26530
ADP + phosphorylated Arabidopsis thaliana proteins AT2G26530
-
-
-
?
ATP + Arabidopsis thaliana protein AT3G11330
ADP + phosphorylated Arabidopsis thaliana proteins AT3G11330
-
-
-
?
ATP + Arabidopsis thaliana protein AT4G38710
ADP + phosphorylated Arabidopsis thaliana proteins AT4G38710
-
-
-
?
ATP + ARF-GAP domain 9
ADP + phosphorylated ARF-GAP domain 9
-
-
-
?
ATP + ASGSPPVPVMHSPPRPVTVK
ADP + ASGSPPVPVMHpSPPRPVTVK
-
-
-
?
ATP + ATMKP1 mitogen-activated protein kinase phosphatase 1
ADP + phosphorylated ATMKP1 mitogen-activated protein kinase phosphatase 1
-
-
-
?
ATP + ATP-dependent RNA helicase
ADP + phosphorylated ATP-dependent RNA helicase
-
-
-
?
ATP + COP1-interacting protein 7
ADP + phosphorylated COP1-interacting protein 7
-
-
-
?
ATP + DLSMNKFDWDHPLHLQPMSPTTVK
ADP + DLSMNKFDWDHPLHLQPMpSPTTVK
-
-
-
?
ATP + epsin N-terminal homology domain-containing protein
ADP + phosphorylated epsin N-terminal homology domain-containing protein
-
-
-
?
ATP + fructose-2,6-bisphosphatase
ADP + phosphorylated fructose-2,6-bisphosphatase
-
-
-
?
ATP + GATA type zinc finger transcription factor family protein
ADP + phosphorylated GATA type zinc finger transcription factor family protein
-
-
-
?
ATP + HVVDEPANEEKPSESSAALSPEK
ADP + HVVDEPANEEKPSESSAALpSPEK
-
-
-
?
ATP + iqd2 IQ-domain 2
ADP + phosphorylated iqd2 IQ-domain 2
-
-
-
?
ATP + iqd32 IQ-domain 31
ADP + phosphorylated iqd32 IQ-domain 31
-
-
-
?
ATP + iqd32 IQ-domain 32
ADP + phosphorylated iqd32 IQ-domain 32
-
-
-
?
ATP + JAL30 PYK10-binding protein 1
ADP + phosphorylated JAL30 PYK10-binding protein 1
-
-
-
?
ATP + mitogen-activated protein kinase phosphatase 1
ADP + phosphorylated mitogen-activated protein kinase phosphatase 1
-
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
-
-
-
?
ATP + NTEEGEMVNNNVSPMMHSR
ADP + NTEEGEMVNNNVpSPMMHSR
-
-
-
?
ATP + NVEKVEEIRSPQTINK
ADP + NVEKVEEIRpSPQTINK
-
-
-
?
ATP + RSYSPGYEGAAAAAPDRDR
ADP + RpSYpSPGYEGAAAAAPDRDR
-
-
-
?
ATP + SAIPDTRPRTPIHESAATGR
ADP + SAIPDTRPRpTPIHESAATGR
-
-
-
?
ATP + SC35-like splicing factor 30
ADP + phosphorylated SC35-like splicing factor 30
-
-
-
?
ATP + SYSPGYEGAAAAAPDRDR
ADP + phosphorylated SYpSPGYEGAAAAAPDRDR
-
-
-
?
ATP + TPSRLAGMFSGTQDK
ADP + pTPpSRLAGMFSGTQDK
-
-
-
?
ATP + trehalose-6-phosphatase synthase S8
ADP + phosphorylated trehalose-6-phosphatase synthase S8
-
-
-
?
ATP + TTFVPSTPPALKTTFVPSTPPALK
ADP + TTFVPpSpTPPALKTTFVPpSTPPALK
-
-
-
?
ATP + TVANSPEALQSPHSSESAFALK
ADP + TVANSPEALQpSPHSSESAFALK
-
-
-
?
ATP + VVEEKPASPEPVKAEAEKPVEK
ADP + VVEEKPApSPEPVKAEAEKPVEK
-
-
-
?
ATP + WRKY25
ADP + phosphorylated WRKY25
-
the transcription factor is an in vitro substrate of MPK4
-
-
?
ATP + WRKY33
ADP + phosphorylated WRKY33
-
the transcription factor is an in vitro substrate of MPK4
-
-
?
ATPgammaS + myelin basic protein
ADP + thiophosphorylated myelin basic protein
Arabidopsis thaliana MPKs use ATPgammaS to thiophosphorylate myelin basic protein
-
-
?
N6-benzyl-ATPgammaS + myelin basic protein
ADP + benzylthiophosphorylated myelin basic protein
Arabidopsis thaliana MPK3 mutant T119A uses N6-benzyl-ATPgammaS to thiophosphorylate myelin basic protein
-
-
?
ATP + BES1
ADP + phosphorylated BES1
i.e. brassinosteroid insensitive1-ethyl methanesulfonate-suppressor1, an Arabidospis thaliana transcription factor. S286 and S137 residues are required for flg22-induced BES1 full phosphorylation in vivo, in which S286 plays a greater role than S137
-
-
?
ATP + BES1
ADP + phosphorylated BES1
i.e. brassinosteroid insensitive1-ethyl methanesulfonate-suppressor1, an Arabidospis thaliana transcription factor
-
-
?
ATP + EB1c
ADP + phosphorylated EB1c
the microtubule plus end protein
-
-
?
ATP + EB1c
ADP + phosphorylated EB1c
the microtubule plus end protein, recombinant GST-tagged substrate protein, phosphorylation on a threonine residue
-
-
?
ATP + MKS1
ADP + phosphorylated MSK1
-
MPK4 acts as a regulator of pathogen defense responses and is required for repression of salicylic acid-dependent resistance and for activation of jasmonate-dependent defense gene expression via MSK1, which interacts with the transcription factors WRKY25 and WRKY33
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
-
MPK6 interacts with gamma-tubulin and co-sediments with plant microtubules polymerized in vitro. The active form of MAP kinase is enriched with microtubules and follows similar dynamics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition
-
-
?
additional information
?
-
MPK6 interacts with gamma-tubulin and co-sediments with plant microtubules polymerized in vitro. The active form of MAP kinase is enriched with microtubules and follows similar dynamics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition
-
-
?
additional information
?
-
-
no activity with recombinant GST-tagged EB1a protein. MPK6 is recruited to gamma-tubulin or gamma-tubulin complexes, but no direct phosphorylation of either gamma-tubulin or gamma-tubulin complex protein GCP4 wby MPK6 is detectable in vitro
-
-
?
additional information
?
-
no activity with recombinant GST-tagged EB1a protein. MPK6 is recruited to gamma-tubulin or gamma-tubulin complexes, but no direct phosphorylation of either gamma-tubulin or gamma-tubulin complex protein GCP4 wby MPK6 is detectable in vitro
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + Arabidopsis thaliana protein AT1G7815
ADP + phosphorylated Arabidopsis thaliana proteins AT1G78150
-
-
-
?
ATP + Arabidopsis thaliana protein AT2G26530
ADP + phosphorylated Arabidopsis thaliana proteins AT2G26530
-
-
-
?
ATP + Arabidopsis thaliana protein AT3G11330
ADP + phosphorylated Arabidopsis thaliana proteins AT3G11330
-
-
-
?
ATP + Arabidopsis thaliana protein AT4G38710
ADP + phosphorylated Arabidopsis thaliana proteins AT4G38710
-
-
-
?
ATP + BES1
ADP + phosphorylated BES1
i.e. brassinosteroid insensitive1-ethyl methanesulfonate-suppressor1, an Arabidospis thaliana transcription factor. S286 and S137 residues are required for flg22-induced BES1 full phosphorylation in vivo, in which S286 plays a greater role than S137
-
-
?
ATP + EB1c
ADP + phosphorylated EB1c
the microtubule plus end protein
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
-
-
-
?
ATP + Arabidopsis thaliana protein AT1G7815
ADP + phosphorylated Arabidopsis thaliana proteins AT1G78150
-
-
-
?
ATP + Arabidopsis thaliana protein AT2G26530
ADP + phosphorylated Arabidopsis thaliana proteins AT2G26530
-
-
-
?
ATP + Arabidopsis thaliana protein AT3G11330
ADP + phosphorylated Arabidopsis thaliana proteins AT3G11330
-
-
-
?
ATP + Arabidopsis thaliana protein AT4G38710
ADP + phosphorylated Arabidopsis thaliana proteins AT4G38710
-
-
-
?
ATP + MKS1
ADP + phosphorylated MSK1
-
MPK4 acts as a regulator of pathogen defense responses and is required for repression of salicylic acid-dependent resistance and for activation of jasmonate-dependent defense gene expression via MSK1, which interacts with the transcription factors WRKY25 and WRKY33
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
-
-
-
?
ATPgammaS + myelin basic protein
ADP + thiophosphorylated myelin basic protein
Arabidopsis thaliana MPKs use ATPgammaS to thiophosphorylate myelin basic protein
-
-
?
N6-benzyl-ATPgammaS + myelin basic protein
ADP + benzylthiophosphorylated myelin basic protein
Arabidopsis thaliana MPK3 mutant T119A uses N6-benzyl-ATPgammaS to thiophosphorylate myelin basic protein
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
-
MPK6 interacts with gamma-tubulin and co-sediments with plant microtubules polymerized in vitro. The active form of MAP kinase is enriched with microtubules and follows similar dynamics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition
-
-
?
additional information
?
-
MPK6 interacts with gamma-tubulin and co-sediments with plant microtubules polymerized in vitro. The active form of MAP kinase is enriched with microtubules and follows similar dynamics to gamma-tubulin, moving from poles to midzone during the anaphase-to-telophase transition
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
additional information
?
-
-
FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ATPgammaS
MPK6 accepts ATPgammaS as a phosphoryldonor to thiophosphorylate myelin basic protein and supposedly also other substrate proteins
ATPgammaS
MPK3 accepts ATPgammaS as a phosphoryldonor to thiophosphorylate myelin basic protein and supposedly also other substrate proteins
ATPgammaS
MPK4 accepts ATPgammaS as a phosphoryldonor to thiophosphorylate myelin basic protein and supposedly also other substrate proteins
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
U0126
the activity of MAPKs is inhibited by treatment of cells with the selective MEK inhibitor
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
pathogen-associated molecular patterns (PAMPs) are recognized by plant pattern recognition receptors to activate PAMP-triggered immunity. PAMP perception enhances phosphorylation of BES1. Flagellin perception, e.g. of Flg22, enhances BES1 phosphorylation. Enzyme MKK5 phosphorylates and activates MPK6
-
additional information
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK6 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK6 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK6 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
-
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK6 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK3 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK3 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK3 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
-
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK3 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK4 is activated by constitutively active MKK1 and MKK2, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK4 is activated by constitutively active MKK1 and MKK2, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK4 is activated by constitutively active MKK1 and MKK2, which cause a dual phosphorylation of the TEY motif in the activation loop
-
additional information
-
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK4 is activated by constitutively active MKK1 and MKK2, which cause a dual phosphorylation of the TEY motif in the activation loop
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
MPK6 is associated with gamma-tubulin on microtubules in proliferating cells
ERK is associated with gamma-tubulin on microtubules in proliferating cells
additional information
additional information
significantly present in all organs except seeds
additional information
significantly present in all organs except seeds
additional information
-
significantly present in all organs except seeds
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
mitotic microtubules, MPK6 is recruited to gamma-tubulin or gamma-tubulin complexes
additional information
additional information
-
co-localization of MPK6 and phosphorylated ERK with gamma-tubulin and with microtubule plus end protein EB1c in Arabidopsis cells
-
additional information
co-localization of MPK6 and phosphorylated ERK with gamma-tubulin and with microtubule plus end protein EB1c in Arabidopsis cells
-
additional information
-
co-localization of MPK6 and p-ERK with gamma-tubulin and with microtubule plus end protein EB1c in Arabidopsis cells. gamma-Tubulin and ERK partly colocalize on the spindle and prominent close to kinetochores. p-ERK follows the dynamic localization of gamma-tubulin in the anaphase-to-telophase transition in Arabidopsis cells. In anaphase, phosphorylated ERK and EB1c-GFP co-localize on shortening kinetochore fibres
-
additional information
co-localization of MPK6 and p-ERK with gamma-tubulin and with microtubule plus end protein EB1c in Arabidopsis cells. gamma-Tubulin and ERK partly colocalize on the spindle and prominent close to kinetochores. p-ERK follows the dynamic localization of gamma-tubulin in the anaphase-to-telophase transition in Arabidopsis cells. In anaphase, phosphorylated ERK and EB1c-GFP co-localize on shortening kinetochore fibres
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
pathogen-associated molecular patterns (PAMPs) are recognized by plant pattern recognition receptors to activate PAMP-triggered immunity. PAMP perception enhances phosphorylation of BES1. BES1 is a unique direct substrate of MPK6 in PAMP-triggered immunity signaling. MAPK-mediated BES1 phosphorylation is another BES1 modulation mechanism in plant cell signaling, in addition to GSK3-like kinase-mediated BES1 phosphorylation and F box protein-mediated BES1 degradation. BES1 phosphorylation induced by flg22 occurs downstream of MAPK activation. MKK5K99M acts as a dominant negative mutant that partially blocks MAPK activation and downstream PTI signaling
physiological function
malfunction
additional information
malfunction
MKKs 4 and 5 induce dual phosphorylation of the TEY motif in the activation loop of the kinase-death version of MPK6KD, but do not stimulate MPK6KD to phosphorylate myelin basic protein
malfunction
root elongation in seedlings of the loss-of-function mutants mpk6-2 and mpk6-3 is less sensitive to NaCl or Na-glutamate, but not to KCl or mannitol, as compared with that of wild-type seedlings. The tolerance of mpk6 to Na+ toxicity is Ca2+-dependent. At the plasma membrane, increased concentrations of NaCl increase the inward Na+-conducted currents while decreasing the outward Na+-conducted currents in wild-type root cells, attended by a positive shift in membrane potential. In mpk6 mutant root cells, NaCl significantly increases outward but not inward Na+-conducted currents, accompanied by a negative shift in membrane potential. Mutant mpk6 decreases NaCl-induced the Na+ accumulation by modifying PM Na+ flux in root cells. Mutant mpk6 accumulates less Na+ in response to NaCl because of the increased cytosolic Ca2+ level in root cells; thus, its root elongation is less inhibited than that of WT by NaCl
malfunction
the mpk6-2 mutant is sensitive to 3-nitro-L-tyrosine (NO2-Tyr) treatment with respect to mitotic abnormalities, and root cells overexpressing the MAP kinase-inactivating phosphatase AP2C3 show defects in chromosome segregation and spindle orientation
physiological function
brassinosteroid insensitive1-ethyl methanesulfonate-suppressor1, BES1, is phosphorylted by Arabidospis thaliana MPK6. MAPK-mediated phosphorylation alters BES1 subcellular accumulation in PAMP-triggered immunity
physiological function
mitogen-activated protein kinase 6 controls root growth in Arabidopsis thaliana by modulating Ca2+-based Na+ flux in root cell under salt stress
physiological function
the active form of MAP kinase interacts with gamma-tubulin on specific subsets of mitotic microtubules during late mitosis. MPK6 phosphorylates EB1c, but not EB1a, and has a role in maintaining regular planes of cell division under stress conditions. MPK6 activtes the micotubule-associated protein MAP65-1 that has a redundant function in Arabidopsis with MAP65-3 in cytokinesis. MPK6 is required for regulation of the alignment of cell division on NO2-Tyr treatment
malfunction
MKKs 1 and 2 induce dual phosphorylation of the TEY motif in the activation loop of the kinase-death version of MPK4KD, but do not stimulate MPK4KD to phosphorylate myelin basic protein
malfunction
MKKs 4 and 5 induce dual phosphorylation of the TEY motif in the activation loop of the kinase-death version of MPK3KD, but do not stimulate MPK3KD to phosphorylate myelin basic protein
additional information
residue Y144 is the gatekeeper in the MPK6 enzyme structure
additional information
residue Y144 is the gatekeeper in the MPK6 enzyme structure
additional information
residue Y144 is the gatekeeper in the MPK6 enzyme structure
additional information
-
residue Y144 is the gatekeeper in the MPK6 enzyme structure
additional information
residue Y119 is the gatekeeper in the MPK3 enzyme structure
additional information
residue Y119 is the gatekeeper in the MPK3 enzyme structure
additional information
residue Y119 is the gatekeeper in the MPK3 enzyme structure
additional information
-
residue Y119 is the gatekeeper in the MPK3 enzyme structure
additional information
residue Y124 is the gatekeeper in the MPK4 enzyme structure
additional information
residue Y124 is the gatekeeper in the MPK4 enzyme structure
additional information
residue Y124 is the gatekeeper in the MPK4 enzyme structure
additional information
-
residue Y124 is the gatekeeper in the MPK4 enzyme structure
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MPK6_ARATH
395
0
45058
Swiss-Prot
other Location (Reliability: 2)
PDB
SCOP
CATH
UNIPROT
ORGANISM
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
residue Y144 is the gatekeeper in MPK6, three-dimensional structure analysis
additional information
residue Y144 is the gatekeeper in MPK6, three-dimensional structure analysis
additional information
residue Y144 is the gatekeeper in MPK6, three-dimensional structure analysis
additional information
-
residue Y144 is the gatekeeper in MPK6, three-dimensional structure analysis
additional information
residue Y119 is the gatekeeper in MPK3, three-dimensional structure analysis
additional information
residue Y119 is the gatekeeper in MPK3, three-dimensional structure analysis
additional information
residue Y119 is the gatekeeper in MPK3, three-dimensional structure analysis
additional information
-
residue Y119 is the gatekeeper in MPK3, three-dimensional structure analysis
additional information
residue Y124 is the gatekeeper in MPK4, three-dimensional structure analysis
additional information
residue Y124 is the gatekeeper in MPK4, three-dimensional structure analysis
additional information
residue Y124 is the gatekeeper in MPK4, three-dimensional structure analysis
additional information
-
residue Y124 is the gatekeeper in MPK4, three-dimensional structure analysis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
phosphoprotein
phosphoprotein
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK6 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
phosphoprotein
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK3 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop
phosphoprotein
the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK4 is activated by constitutively active MKK1 and MKK2, which cause a dual phosphorylation of the TEY motif in the activation loop
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
generation of the T-DNA insertion lines mpk6-2 (Salk 073907) and mpk6-3(Salk 127507), that are loss-of-function mutants, the mutants are less sensitive to NaCl or Na-glutamate, but not to KCl or mannitol, as compared with that of wild-type seedlings
additional information
-
overexpression of MPK4 substrate MSK1 in wild-type plants leads to activated salicylic-dependent resistance, but does not interfere with induction of a defense gene by jasmonate
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant GST-tagged MPK6 by affinity chromatography from Escherichia coli
recombinant His-tagged MPK6 by metal affinity chromatography, the His-tag is cleaved off by TEV protease
recombinant GST-tagged MPK3 by affinity chromatography from Escherichia coli
recombinant GST-tagged MPK4 by affinity chromatography from Escherichia coli
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant expression of GST-tagged MPK6 in Escherichia coli
recombinant expression of GST-tagged MPK6, coexpression of His-tagged BES1 and GST-tagged MPK6 in Nicotiana benthamiana, interaction analysis
gene mpk4, gene expression profiling, overexpression of mpk4 leads to increased expression of the genes PR1 and PR2, encoding plant defense proteins, and of the ICS1 gene, encoding a salicylic acid biosynthetic enzyme
-
recombinant expression of GST-tagged MPK3 in Escherichia coli
recombinant expression of GST-tagged MPK4 in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Theologis, A.; Ecker, J.R.; Palm, C.J.; et al.
Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana
Nature
408
816-820
2000
Arabidopsis thaliana (Q39021), Arabidopsis thaliana (Q39022)
Lin, X.; Kaul, S.; Rounsley, S.; Shea, T.P.; Benito, M.I.; Town, C.D.; Fujii, C.Y.; Mason, T.; Bowman, C.L.; Barnstead, M.; Feldblyum, T.V.; Buell, C.R.; Ketchum, K.A.; Lee, J.; Ronning, C.M.; Koo, H.L.; Moffat, K.S.; Cronin, L.A.; Shen, M.; Pai, G.; Van Aken, S.; Umayam, L.; Tallon, L.J.; Gill, J.E.; Venter, J.C.; et al.
Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana
Nature
402
761-768
1999
Arabidopsis thaliana (Q39026), Arabidopsis thaliana (Q39027)
Mizoguchi, T.; Gotoh, Y.; Nishida, E.; Yamaguchi-Shinozaki, K.; Hayashida, N.; Iwasaki, T.; Kamada, H.; Shinozaki, K.
Characterization of two cDNAs that encode MAP kinase homologues in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cells
Plant J.
5
111-122
1994
Arabidopsis thaliana (Q39021), Arabidopsis thaliana (Q39022), Arabidopsis thaliana
Mizoguchi, T.; Hayashida, N.; Yamaguchi-Shinozaki, K.; Kamada, H.; Shinozaki, K.
ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana
FEBS Lett.
336
440-444
1993
Arabidopsis thaliana (Q39023), Arabidopsis thaliana (Q39024), Arabidopsis thaliana (Q39025), Arabidopsis thaliana (Q39026), Arabidopsis thaliana (Q39027)
Andreasson, E.; Jenkins, T.; Brodersen, P.; Thorgrimsen, S.; Petersen, N.H.; Zhu, S.; Qiu, J.L.; Micheelsen, P.; Rocher, A.; Petersen, M.; Newman, M.A.; Bjorn Nielsen, H.; Hirt, H.; Somssich, I.; Mattsson, O.; Mundy, J.
The MAP kinase substrate MKS1 is a regulator of plant defense responses
EMBO J.
24
2579-2589
2005
Arabidopsis thaliana
Leissing, F.; Nomoto, M.; Bocola, M.; Schwaneberg, U.; Tada, Y.; Conrath, U.; Beckers, G.J.
Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases
BMC Plant Biol.
16
48
2016
Arabidopsis thaliana (Q39023), Arabidopsis thaliana (Q39024), Arabidopsis thaliana (Q39026), Arabidopsis thaliana
Han, S.; Wang, C.; Wang, W.; Jiang, J.
Mitogen-activated protein kinase 6 controls root growth in Arabidopsis by modulating Ca2+-based Na+ flux in root cell under salt stress
J. Plant Physiol.
171
26-34
2014
Arabidopsis thaliana (Q39026), Arabidopsis thaliana Col-0 (Q39026)
Kohoutova, L.; Kourova, H.; Nagy, S.K.; Volc, J.; Halada, P.; Meszaros, T.; Meskiene, I.; Boegre, L.; Binarova, P.
The Arabidopsis mitogen-activated protein kinase 6 is associated with gamma-tubulin on microtubules, phosphorylates EB1c and maintains spindle orientation under nitrosative stress
New Phytol.
207
1061-1074
2015
Arabidopsis thaliana, Arabidopsis thaliana (Q39026)
Kang, S.; Yang, F.; Li, L.; Chen, H.; Chen, S.; Zhang, J.
The Arabidopsis transcription factor brassinosteroid insensitive1-ethyl methanesulfonate-suppressor1 is a direct substrate of mitogen-activated protein kinase6 and regulates immunity
Plant Physiol.
167
1076-1086
2015
Arabidopsis thaliana (Q39026), Arabidopsis thaliana Col-0 (Q39026)
Rayapuram, N.; Bigeard, J.; Alhoraibi, H.; Bonhomme, L.; Hesse, A.; Vinh, J.; Hirt, H.; Pflieger, D.
Quantitative phosphoproteomic analysis reveals shared and specific targets of Arabidopsis mitogen-activated protein kinases (MAPKs) MPK3, MPK4, and MPK6
Mol. Cell. Proteomics
17
61-80
2018
Arabidopsis thaliana (Q39023), Arabidopsis thaliana (Q39024), Arabidopsis thaliana (Q39026)
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