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Information on EC 2.7.11.18 - myosin-light-chain kinase and Organism(s) Homo sapiens and UniProt Accession Q9H1R3

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IUBMB Comments
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
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This record set is specific for:
Homo sapiens
UNIPROT: Q9H1R3
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
mlck, myosin light chain kinase, myosin light-chain kinase, mlc kinase, smooth muscle myosin light chain kinase, smmlck, nmmlck, myosin kinase, cmlck, skmlck, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+-calmodulin-dependent myosin light chain kinase
-
MLCK2
isoform
myosin light chain kinase 2, skeletal/cardiac muscle
-
Ca2+-calmodulin-dependent myosin light chain kinase
-
calcium/calmodulin-dependent myosin light chain kinase
-
-
-
-
cardiac-MLCK
-
-
cardiac-specific myosin regulatory light chain kinase
-
-
cMLCK
-
-
endothelial cell myosin light chain kinase
-
-
endothelial MLCK
-
-
endothelial myosin light chain kinase
-
-
kinase, myosin light-chain (phosphorylating)
-
-
-
-
long myosin light chain kinase
-
-
MLCK1
isoform
myosin kinase
-
-
-
-
myosin light chain kinase
myosin light chain kinase, smooth muscle and non-muscle isozymes
-
myosin light chain protein kinase
-
-
-
-
myosin light-chain kinase
myosine light chain kinase
-
-
nmMLCK1
-
non-muscle myosin light chain kinase variant
nmMLCK2
-
-
non-muscle myosin light chain kinase
-
-
obscurin-associated kinase
-
-
obscurin-MLCK-like kinase
-
-
SMM light chain kinase
-
smooth muscle myosin light chain kinase
smooth-muscle myosin light chain kinase
-
-
smooth-muscle-myosin-light-chain kinase
-
-
-
-
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:[myosin light chain] O-phosphotransferase
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
51845-53-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + TRPC5 channel
ADP + phosphorylated TRPC5 channel
show the reaction diagram
Ca2+/calmodulin-dependent myosin light chain kinase is essential for activation of plasma membrane TRPC5 channels, responsible for Ca2+ influx, from Drosophila melanogaster expressed in HEK-293 cells, phosphorylation sites are Ser698, Ser775, and Thr700, mutational analysis
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + H-KKRAARATSNVFA-NH2
ADP + ?
show the reaction diagram
-
peptide substrate
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
ATP + obscurin
ADP + obscurin phosphate
show the reaction diagram
-
-
-
-
?
ATP + TRPC5 channel
ADP + phosphorylated TRPC5 channel
show the reaction diagram
Ca2+/calmodulin-dependent myosin light chain kinase is essential for activation of plasma membrane TRPC5 channels, responsible for Ca2+ influx, from Drosophila melanogaster expressed in HEK-293 cells, phosphorylation sites are Ser698, Ser775, and Thr700, mutational analysis
-
-
?
ATP + [myosin II regulatory light chain]
ADP + [myosin II regulatory light chain] phosphate
show the reaction diagram
ATP + [myosin light chain 2a]
ADP + [myosin light chain 2a] phosphate
show the reaction diagram
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
ATP + [ventricular isoform of myosin regulatory light chain MLC2v]
ADP + [ventricular isoform of myosin regulatory light chain MLC2v] phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
ATP + obscurin
ADP + obscurin phosphate
show the reaction diagram
-
-
-
-
?
ATP + [myosin II regulatory light chain]
ADP + [myosin II regulatory light chain] phosphate
show the reaction diagram
-
involved in many cellular cytoskeletal functions
-
-
?
ATP + [myosin light chain 2a]
ADP + [myosin light chain 2a] phosphate
show the reaction diagram
-
MLC phosphorylation plays a regulatory role in inotropic response to alpha1-adrenergic stimulation in the heart, enzyme inhibition causes heart failure in ventricular trabeculae
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
ATP + [ventricular isoform of myosin regulatory light chain MLC2v]
ADP + [ventricular isoform of myosin regulatory light chain MLC2v] phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
activates, dependent on
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-(5-Iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride
inhibitor targeting the ATPase domain of the enzyme
-
ML-9
impairs the plasma membrane localization of TRPC5 channels
RKKYKYRRK-NH2
specific enzyme inhibitor targeting the kinase domain
1-(5-Iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride
inhibitor targeting the ATPase domain of the enzyme
-
bosentan
-
-
-
cimetidine
-
-
diclofenac
-
-
EGTA
-
-
entacapone
-
-
human arrest defective-1
-
human arrest defective-1 is a bona fide regulator of MLCK, human arrest defective-1 associates with an IgG motif of MLCK and acetylates the Lys-608 residue in this motif and by so doing deactivates MLCK
-
KKRAARATS-NH2
-
i.e. KKRAARATS amide
KT5926
-
i.e. (8R,9S,11S)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-14-n-propoxy-2,3,9,10-tetrahydro-8,11-epoxy,1H,8H,11H-2,7beta,11alpha-triazadeibenzo[a,g]cycloocta[cde]triden-1-one, specific inhibitor
phosphorylation
-
-
-
RKKYKYRRK-NH2
tacrolimus
-
-
tolcapone
-
-
W-7
-
40-60% inhibition in vivo
Wortmannin
-
complete inhibition at 0.01 mM
Y-27632
-
40-60% inhibition in vivo
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin
activates, dependent on
tumor necrosis factor-like 1A
-
-
Calmodulin
eotaxin
-
activates the enzyme 4fold, induces enzyme phosphorylation
-
interleukin 1
-
i.e IL-1, activates enzyme activity in endothelial cells
-
isoprenaline
-
80% activation at up to 0.01 mM
phenylephrine
-
80% activation at up to 0.1 mM
thrombin
-
stimulates endothelial isozyme activity in endothelium, and increases EC MLCK interaction with macrophage migration inhibition factor MIF
-
TNFalpha
-
stimulates endothelial isozyme activity in endothelium, and increases EC MLCK interaction with macrophage migration inhibition factor MIF
-
tumor necrosis factor-alpha
-
i.e TNF-alpha, activates enzyme activity in endothelial cells
-
tumor necrosis factor-like 1A
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.121
ATP
-
23-25°C, pH 7.5
0.018
myosin light chain
-
23-25°C, pH 7.5
0.005
[ventricular isoform of myosin regulatory light chain MLC2v]
-
at 25°C, pH not specified in the publication
-
additional information
additional information
-
kinetic constants for enzymes from various sources with different myosin light chains as substrates
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.17
myosin light chain
-
23-25°C, pH 7.5
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029
RKKYKYRRK-NH2
Homo sapiens
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
assay at
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at
23 - 25
-
assay at
25
assay at
30
-
assay at
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
endobronchial tissue
Manually annotated by BRENDA team
-
primary cell culture
Manually annotated by BRENDA team
-
a cerebral microvascular endothelial cell line
Manually annotated by BRENDA team
well differentiated cells, isozyme MLCK1
Manually annotated by BRENDA team
-
ileal epithelial MLCK expression is mildly upregulated in inactive inflammatory bowel disease, and highly upregulated in active IBD
Manually annotated by BRENDA team
-
LNCaP cells express both long (non-muscle) and short (smooth muscle) isoforms of MLCK
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
expression of obscurin-MLCK-like kinase is restricted to muscles in the myonucleus
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MLCK and the N-terminal 75 residues of MLCK move on actin bundles and stress fibers of smooth muscle cell cytoskeletons by a random one-dimensional diffusion mechanism, modelling, overview. MLCK motion is permitted only if acto–myosin and MLCK–myosin interactions are weak
Manually annotated by BRENDA team
-
associated
Manually annotated by BRENDA team
-
exclusively
Manually annotated by BRENDA team
-
associated
Manually annotated by BRENDA team
isozyme MLCK2
-
Manually annotated by BRENDA team
additional information
isozyme MLCK1 is predominantly located in the perijunctional actomyosin ring in villus enterocytes
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
non-muscle myosin light chain kinase (nmMLCK) mutations P21H, S147P, and V261A (all located to loops that connect the immunoglobulin-like domains of nmMLCK) alter the enzyme conformation and the N-terminal amino acid sequence of the non-muscle isoform of MLCK and are highly associated with susceptibility to acute lung injury and asthma, especially in individuals of African descent
physiological function
additional information
-
structure-function analysis of the non-muscle myosin light chain kinase (nmMLCK) isoform by NMR spectroscopy and molecular modeling, overview. The two major nmMLCK splice variants, nmMLCK1 (about 211 kDa) and nmMLCK2 (about 203 kDa), differ by 69 amino acids within exon 11 (partially deleted in nmMLCK2), a stretch that contains the highly phosphorylated Y464 and Y471, suggesting that nmMLCK splice variants are differentially regulated by Tyr phosphorylation. Potential involvement of S147P and P21H SNP sites in nmMLCK1 in phosphorylation-dependent binding of 14-3-3 proteins
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MYLK2_HUMAN
596
0
64685
Swiss-Prot
other Location (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
210000
gel filtration
105000
-
x * 105000, SDS-PAGE
130000 - 150000
-
smooth muscle isozyme
150000
-
-
190000
210000
211000
deduced from nucleotide sequence
214000
215000
-
-
additional information
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
phosphoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A27F/A29R/A31R/A32F
-
mutant enzyme with defective ATP binding site, mutant leads to nearly unaltered MLC phosphorylation activity and altered morphology in transfected CV1 cells
H21P
-
naturally occuring single nucleotide polymorphism increasing the risk for acute lung injury within 5 das of traumatic insult
P147S
-
naturally occuring single nucleotide polymorphism increasing the risk for acute lung injury within 5 das of traumatic insult
P21H
-
site-directed mutagenesis, alters the N-terminal amino acid sequence of the non-muscle isoform of MLCK (nmMLCK) and is highly associated with susceptibility to acute lung injury and asthma, especially in individuals of African descent, the mutation causes minimal conformational changes compared to the wild-type enzyme
S147P
-
site-directed mutagenesis, alters the N-terminal amino acid sequence of the non-muscle isoform of MLCK (nmMLCK) and is highly associated with susceptibility to acute lung injury and asthma, especially in individuals of African descent, the mutation causes minimal conformational changes compared to the wild-type enzyme
V261A
-
site-directed mutagenesis, alters the N-terminal amino acid sequence of the non-muscle isoform of MLCK (nmMLCK) and is highly associated with susceptibility to acute lung injury and asthma, especially in individuals of African descent, the mutation causes minimal conformational changes compared to the wild-type enzyme
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
protease inhibitors stabilize during purification
-
repeated freeze-thawing decreases activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial, affinity chromatography on calmodulin-Sepharose
-
partially from eosinophils
-
purified to homogeneity from a number of vertebrate muscles and partially purified from non-muscle tissues
-
recombinant GFP-fusion enzyme from HeLa cells
-
recombinant GST-fusion isozyme EC MLCK by affinity chromatgraphy
-
recombinant wild-type and mutant enzymes from transfected bovine endothelial cells
-
recombinant wild-type and mutant isozymes nmMLCK1 from Escherichia coli strain BL21(DE3) by chromatography on a chitin column and dialysis
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expressionin HEK-293 cells, co-expression with TRPC5 from Drosophila melanogaster
DNA and amino acid sequence determination and analysis, expressionin HEK-293 cells, co-expression with TRPC5 from Drosophila melanogaster
DNA sequence determination and analysis of splicing variant 1 of long endothelial enzyme, large scale expression of endothelial isozyme MLCK1 in Spodoptera frugiperda Sf9 cells via Baculovirus transfection system
DNA sequence determination and analysis of splicing variant 2 of long endothelial enzyme, large scale expression of endothelial isozyme MLCK2 in Spodoptera frugiperda Sf9 cells via Baculovirus transfection system
DNA sequence determination and analysis, overexpression in Escherichia coli strain XL-1 Blue
-
expression as His-tagged protein
-
expression of calmodulin-independent mutant MLCK1745 and of MLVKATPdel mutant in African green monkey kidney CV1 fibroblasts as V5-epitope- and His-tagged proteins
-
expression of calmodulin-independent mutant MLCK1745, lacking the C-terminal part including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, and of MLVKATPdel mutant with a defective ATP-binding site in bovine pulmonary artery endothelial cells BPAEC as V5-epitope-tagged proteins
-
expression of epitope-tagged endothelial isozyme in endothelial cells, expression of GST-fusion isozyme EC MLCK in bacteria, expression of endothelial iszyme EC MLCK and macrophage migration inhibition factor MIF in the yeast two-hybrid system, overview
-
expression of GFP-tagged enzyme in HeLa cells
-
gene MYLK, DNA and amino acid sequence determination and analysis of wild-type and single nucleotide mutants, genotyping
-
gene MYLK, recombinant expression of wild-type and truncated enzyme, the latter comprising the N-terminal 75 residues of MLCK
genes MYLK2 and MYLK3, DNA and amino acid sequence determination and analysis, expression analysis in heart, sequence comparisons
-
obscurin-associated kinase, DNA and amino acid sequence determination, sequence and expression analysis and comparison, both the mouse and human obscurinassociated kinase-like transcripts also share a conserved putative translation initiation site, overview. Expression in Escherichia coli strain BL21(DE3)
-
stable expression of a constructed Ca2+/calmodulin-dependent fluorescent biosensor MLCK in HEK-293 cells
-
two major nmMLCK splice variants, nmMLCK1 (about 211 kDa) and nmMLCK2 (about 203 kDa), differ by 69 amino acids within exon 11 (partially deleted in nmMLCK2), a stretch that contains the highly phosphorylated Y464 and Y471. Recombinant expression of wild-type and mutant isozymes nmMLCK1 in Escherichia coli strain BL21(DE3), recombinant expression of Flag-tagged nmMLCK1, recombinant expression of two variants of the 1-494aa segments, wild type (P21-S147-V261)-1-494-biotin and a single SNP (P147)-1-494-biotin
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the expression levels of splice variant MLCK2 is significantly increased in actively inflamed tissues of the Crohn's disease and ulcerative colitis patient samples. The splice variant MLCK2 is overexpressed in colonic biopsy specimens of inflammatory bowel disease patients. Treatment with low-dose cytokines tumour necrosis factor-like 1A and interferon gamma upregulates isoform MLCK2 transcript
0.1 nM androgen R1881 causes a progressive decrease in MLCK mRNA levels which decreases by 70% and 90%, respectively, at 24 and 48 h
-
10 ng/ml tumour necrosis factor-alpha causes a significant increase of myosin light chain kinase gene activity, tumour necrosis factor-alpha causes an increase in nuclear factor-kappaB to the nuclear factor-kappaB1 binding motif which presumably leads to the activation of MLCK promoter
-
interferon gamma does not upregulate isoform MLCK1 transcript
Lactobacillus rhamnosus alleviates intestinal barrier dysfunction in part by increasing expression of myosin light-chain kinase in vivo
-
rebeccamycin mediates reduction in enzyme production
-
the expression level of the splice variant MLCK1 is significantly increased in actively inflamed tissues of the Crohn's disease and ulcerative colitis patient samples. The splice variant MLCK1 is overexpressed in colonic biopsy specimens of inflammatory bowel disease patients. Treatment with low-dose cytokines tumor necrosis factor-like 1A
the expression of MLCK is increased in endobronchial biopsies from humans with mild asthma
-
tumor necrosis factor-alpha induces an increase in enzyme mRNA expression in intestinal epithelial cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (Q15746)
Manually annotated by BRENDA team
Deloukas, P.; Matthews, L.H.; Ashurst, J.; Burton, J.; Gilbert, J.G.; et al.
The DNA sequence and comparative analysis of human chromosome 20
Nature
414
865-871
2001
Homo sapiens (Q9H1R3)
Manually annotated by BRENDA team
Davis, J.S.; Hassanzadeh, S.; Winitsky, S.; Lin, H.; Satorius, C.; Vemuri, R.; Aletras, A.H.; Wen, H.; Epstein, N.D.
The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation
Cell
107
631-641
2001
Oryctolagus cuniculus (P07313), Homo sapiens (Q9H1R3), Homo sapiens
Manually annotated by BRENDA team
Garcia, J.G.N.; Lazar, V.; Gilbert-McClain, L.I.; Gallagher, P.J.; Verin, A.D.
Myosin light chain kinase in endothelium: molecular cloning and regulation
Am. J. Respir. Cell Mol. Biol.
16
489-494
1997
Bos taurus, Homo sapiens, Homo sapiens (Q15746)
Manually annotated by BRENDA team
Lazar, V.; Garcia, J.G.
A single human myosin light chain kinase gene (MLCK; MYLK)
Genomics
57
256-267
1999
Homo sapiens (Q15746), Homo sapiens
Manually annotated by BRENDA team
Potier, M.C.; Chelot, E.; Pekarsky, Y.; Gardiner, K.; Rossier, J.; Turnell, W.G.
The human myosin light chain kinase (MLCK) from hippocampus: cloning, sequencing, expression, and localization to 3qcen-q21
Genomics
29
562-570
1995
Homo sapiens (Q15746), Homo sapiens
Manually annotated by BRENDA team
Watterson, D.M.; Schavocky, J.P.; Guo, L.; Weiss, C.; Chlenski, A.; Shirinsky, V.P.; Van Eldik, L.J.; Haiech, J.
Analysis of the kinase-related protein gene found at human chromosome 3q21 in a multi-gene cluster: organization, expression, alternative splicing, and polymorphic marker
J. Cell. Biochem.
75
481-491
1999
Homo sapiens (Q15746), Homo sapiens
Manually annotated by BRENDA team
Hathaway, D.R.; Adelstein, R.S.
Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity
Proc. Natl. Acad. Sci. USA
76
1653-1657
1979
Homo sapiens
Manually annotated by BRENDA team
Pato, M.D.; Lye, S.J.; Kerc, E.
Purification and characterization of pregnant sheep myometrium myosin light chain kinase
Arch. Biochem. Biophys.
287
24-32
1991
Ovis aries, Homo sapiens, Meleagris gallopavo
Manually annotated by BRENDA team
Adelstein, R.S.; Klee, C.B.
Purification of smooth muscle myosin light-chain kinase
Methods Enzymol.
85
298-308
1982
Homo sapiens, Meleagris gallopavo
-
Manually annotated by BRENDA team
Conti, M.A.; Adelstein, R.S.
Purification and properties of myosin light chain kinases
Methods Enzymol.
196
34-47
1991
Bos taurus, Oryctolagus cuniculus, Homo sapiens, Meleagris gallopavo, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Stull, J.T.; Nunnally, M.H.; Michnoff, C.H in
Calmoduline-dependent protein kinases
The Enzymes, 3rd. Ed. (Boyer, P. D. , Krebs, E. G. , eds. )
17
113-166
1986
Bos taurus, Canis lupus familiaris, Cavia porcellus, Gallus gallus, Homo sapiens, Meleagris gallopavo, Rattus norvegicus
-
Manually annotated by BRENDA team
Verin, A.D.; Gilbert-McClain, L.I.; Patterson, C.E.; Garcia, J.G.N.
Biochemical regulation of the nonmuscle myosin light chain kinase isoform in bovine endothelium
Am. J. Respir. Cell Mol. Biol.
19
767-776
1998
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Dudek, S.M.; Birukov, K.G.; Zhan, X.; Garcia, J.G.N.
Novel interaction of cortactin with endothelial cell myosin light chain kinase
Biochem. Biophys. Res. Commun.
298
511-519
2002
Homo sapiens
Manually annotated by BRENDA team
Grimm, M.; Haas, P.; Willipinski-Stapelfeldt, B.; Zimmermann, W.H.; Rau, T.; Pantel, K.; Weyand, M.; Eschenhagen, T.
Key role of myosin light chain (MLC) kinase-mediated MLC2a phosphorylation in the alpha 1-adrenergic positive inotropic effect in human atrium
Cardiovasc. Res.
65
211-220
2005
Homo sapiens
Manually annotated by BRENDA team
Dulyaninova, N.G.; Bresnick, A.R.
The long myosin light chain kinase is differentially phosphorylated during interphase and mitosis
Exp. Cell Res.
299
303-314
2004
Oryctolagus cuniculus, Homo sapiens
Manually annotated by BRENDA team
Geguchadze, R.; Zhi, G.; Lau, K.S.; Isotani, E.; Persechini, A.; Kamm, K.E.; Stull, J.T.
Quantitative measurements of Ca(2+)/calmodulin binding and activation of myosin light chain kinase in cells
FEBS Lett.
557
121-124
2004
Homo sapiens
Manually annotated by BRENDA team
Adachi, T.; Stafford, S.; Kayaba, H.; Chihara, J.; Alam, R.
Myosin light chain kinase mediates eosinophil chemotaxis in a mitogen-activated protein kinase-dependent manner
J. Allergy Clin. Immunol.
111
113-116
2003
Homo sapiens
Manually annotated by BRENDA team
Clayburgh, D.R.; Rosen, S.; Witkowski, E.D.; Wang, F.; Blair, S.; Dudek, S.; Garcia, J.G.; Alverdy, J.C.; Turner, J.R.
A differentiation-dependent splice variant of myosin light chain kinase, MLCK1, regulates epithelial tight junction permeability
J. Biol. Chem.
279
55506-55513
2004
Homo sapiens (Q15746)
Manually annotated by BRENDA team
Wadgaonkar, R.; Nurmukhambetova, S.; Zaiman, A.L.; Garcia, J.G.
Mutation analysis of the non-muscle myosin light chain kinase (MLCK) deletion constructs on CV1 fibroblast contractile activity and proliferation
J. Cell. Biochem.
88
623-634
2003
Homo sapiens
Manually annotated by BRENDA team
Wadgaonkar, R.; Linz-McGillem, L.; Zaiman, A.L.; Garcia, J.G.
Endothelial cell myosin light chain kinase (MLCK) regulates TNFalpha-induced NFkappaB activity
J. Cell. Biochem.
94
351-364
2005
Homo sapiens
Manually annotated by BRENDA team
Wadgaonkar, R.; Dudek, S.M.; Zaiman, A.L.; Linz-McGillem, L.; Verin, A.D.; Nurmukhambetova, S.; Romer, L.H.; Garcia, J.G.
Intracellular interaction of myosin light chain kinase with macrophage migration inhibition factor (MIF) in endothelium
J. Cell. Biochem.
95
849-858
2005
Homo sapiens
Manually annotated by BRENDA team
Tinsley, J.H.; Yuan, S.Y.; Wilson, E.
Isoform-specific knockout of endothelial myosin light chain kinase: closing the gap on inflammatory lung disease
Trends Pharmacol. Sci.
25
64-66
2004
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Zhu, H.Q.; Wang, Y.; Hu, R.L.; Ren, B.; Zhou, Q.; Jiang, Z.K.; Gui, S.Y.
Distribution and expression of non-muscle myosin light chain kinase in rabbit livers
World J. Gastroenterol.
9
2715-2719
2003
Homo sapiens, Oryctolagus cuniculus
Manually annotated by BRENDA team
Niggli, V.; Schmid, M.; Nievergelt, A.
Differential roles of Rho-kinase and myosin light chain kinase in regulating shape, adhesion, and migration of HT1080 fibrosarcoma cells
Biochem. Biophys. Res. Commun.
343
602-608
2006
Homo sapiens
Manually annotated by BRENDA team
Dudnakova, T.V.; Stepanova, O.V.; Dergilev, K.V.; Chadin, A.V.; Shekhonin, B.V.; Watterson, D.M.; Shirinsky, V.P.
Myosin light chain kinase colocalizes with nonmuscle myosin IIB in myofibril precursors and sarcomeric Z-lines of cardiomyocytes
Cell Motil. Cytoskeleton
63
375-383
2006
Gallus gallus, Homo sapiens
Manually annotated by BRENDA team
Seguchi, O.; Takashima, S.; Yamazaki, S.; Asakura, M.; Asano, Y.; Shintani, Y.; Wakeno, M.; Minamino, T.; Kondo, H.; Furukawa, H.; Nakamaru, K.; Naito, A.; Takahashi, T.; Ohtsuka, T.; Kawakami, K.; Isomura, T.; Kitamura, S.; Tomoike, H.; Mochizuki, N.; Kitakaze, M.
A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart
J. Clin. Invest.
117
2812-2824
2007
Danio rerio, Homo sapiens
Manually annotated by BRENDA team
Afonso, P.V.; Ozden, S.; Prevost, M.C.; Schmitt, C.; Seilhean, D.; Weksler, B.; Couraud, P.O.; Gessain, A.; Romero, I.A.; Ceccaldi, P.E.
Human blood-brain barrier disruption by retroviral-infected lymphocytes: role of myosin light chain kinase in endothelial tight-junction disorganization
J. Immunol.
179
2576-2583
2007
Homo sapiens
Manually annotated by BRENDA team
Shimizu, S.; Yoshida, T.; Wakamori, M.; Ishii, M.; Okada, T.; Takahashi, M.; Seto, M.; Sakurada, K.; Kiuchi, Y.; Mori, Y.
Ca2+-calmodulin-dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK293 cells
J. Physiol.
570
219-235
2006
Homo sapiens (Q15746), Homo sapiens (Q9H1R3), Homo sapiens
Manually annotated by BRENDA team
Blair, S.A.; Kane, S.V.; Clayburgh, D.R.; Turner, J.R.
Epithelial myosin light chain kinase expression and activity are upregulated in inflammatory bowel disease
Lab. Invest.
86
191-201
2006
Homo sapiens
Manually annotated by BRENDA team
Zhou, X.; Liu, Y.; You, J.; Zhang, H.; Zhang, X.; Ye, L.
Myosin light-chain kinase contributes to the proliferation and migration of breast cancer cells through cross-talk with activated ERK1/2
Cancer Lett.
270
312-327
2008
Homo sapiens (Q15746)
Manually annotated by BRENDA team
Christie, J.D.; Ma, S.F.; Aplenc, R.; Li, M.; Lanken, P.N.; Shah, C.V.; Fuchs, B.; Albelda, S.M.; Flores, C.; Garcia, J.G.
Variation in the myosin light chain kinase gene is associated with development of acute lung injury after major trauma
Crit. Care Med.
36
2794-2800
2008
Homo sapiens
Manually annotated by BRENDA team
Borisov, A.B.; Raeker, M.O.; Russell, M.W.
Developmental expression and differential cellular localization of obscurin and obscurin-associated kinase in cardiac muscle cells
J. Cell. Biochem.
103
1621-1635
2008
Homo sapiens, Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Chilcoat, C.D.; Sharief, Y.; Jones, S.L.
Tonic protein kinase A activity maintains inactive beta2 integrins in unstimulated neutrophils by reducing myosin light-chain phosphorylation: role of myosin light-chain kinase and Rho kinase
J. Leukoc. Biol.
83
964-971
2008
Homo sapiens
Manually annotated by BRENDA team
Zhu, H.Q.; Cheng, X.W.; Xiao, L.L.; Jiang, Z.K.; Zhou, Q.; Gui, S.Y.; Wei, W.; Wang, Y.
Melatonin prevents oxidized low-density lipoprotein-induced increase of myosin light chain kinase activation and expression in HUVEC through ERK/MAPK signal transduction
J. Pineal Res.
45
328-334
2008
Homo sapiens
Manually annotated by BRENDA team
Feighery, L.M.; Cochrane, S.W.; Quinn, T.; Baird, A.W.; OToole, D.; Owens, S.E.; ODonoghue, D.; Mrsny, R.J.; Brayden, D.J.
Myosin light chain kinase inhibition: correction of increased intestinal epithelial permeability in vitro
Pharm. Res.
25
1377-1386
2008
Homo sapiens, Mus musculus, Mus musculus C57BL/6, Rattus norvegicus
Manually annotated by BRENDA team
Moitra, J.; Evenoski, C.; Sammani, S.; Wadgaonkar, R.; Turner, J.R.; Ma, S.F.; Garcia, J.G.
A transgenic mouse with vascular endothelial over-expression of the non-muscle myosin light chain kinase-2 isoform is susceptible to inflammatory lung injury: role of sexual dimorphism and age
Transl. Res.
151
141-153
2008
Homo sapiens
Manually annotated by BRENDA team
Leguillette, R.; Laviolette, M.; Bergeron, C.; Zitouni, N.; Kogut, P.; Solway, J.; Kachmar, L.; Hamid, Q.; Lauzon, A.M.
Myosin, transgelin, and myosin light chain kinase: expression and function in asthma
Am. J. Respir. Crit. Care Med.
179
194-204
2009
Homo sapiens
Manually annotated by BRENDA team
Ye, D.; Ma, T.Y.
Cellular and molecular mechanisms that mediate basal and tumour necrosis factor-alpha-induced regulation of myosin light chain kinase gene activity
J. Cell. Mol. Med.
12
1331-1346
2008
Homo sapiens
Manually annotated by BRENDA team
Miyauchi, E.; Morita, H.; Tanabe, S.
Lactobacillus rhamnosus alleviates intestinal barrier dysfunction in part by increasing expression of zonula occludens-1 and myosin light-chain kinase in vivo
J. Dairy Sci.
92
2400-2408
2009
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Leveille, N.; Fournier, A.; Labrie, C.
Androgens down-regulate myosin light chain kinase in human prostate cancer cells
J. Steroid Biochem. Mol. Biol.
114
174-179
2009
Homo sapiens
Manually annotated by BRENDA team
Brown, M.; Adyshev, D.; Bindokas, V.; Moitra, J.; Garcia, J.G.; Dudek, S.M.
Quantitative distribution and colocalization of non-muscle myosin light chain kinase isoforms and cortactin in human lung endothelium
Microvasc. Res.
80
75-88
2010
Homo sapiens
Manually annotated by BRENDA team
Shin, D.H.; Chun, Y.S.; Lee, K.H.; Shin, H.W.; Park, J.W.
Arrest defective-1 controls tumor cell behavior by acetylating myosin light chain kinase
PLoS ONE
4
e7451
2009
Homo sapiens
Manually annotated by BRENDA team
Hong, F.; Brizendine, R.K.; Carter, M.S.; Alcala, D.B.; Brown, A.E.; Chattin, A.M.; Haldeman, B.D.; Walsh, M.P.; Facemyer, K.C.; Baker, J.E.; Cremo, C.R.
Diffusion of myosin light chain kinase on actin: A mechanism to enhance myosin phosphorylation rates in smooth muscle
J. Gen. Physiol.
146
267-280
2015
Homo sapiens (Q15746)
Manually annotated by BRENDA team
Shen, K.; Ramirez, B.; Mapes, B.; Shen, G.R.; Gokhale, V.; Brown, M.E.; Santarsiero, B.; Ishii, Y.; Dudek, S.M.; Wang, T.; Garcia, J.G.
Structure-function analysis of the non-muscle myosin light chain kinase (nmMLCK) isoform by NMR spectroscopy and molecular modeling: influence of MYLK variants
PLoS ONE
10
e0130515
2015
Homo sapiens
Manually annotated by BRENDA team
Watari, A.; Sakamoto, Y.; Hisaie, K.; Iwamoto, K.; Fueta, M.; Yagi, K.; Kondoh, M.
Rebeccamycin attenuates TNF-alpha-induced intestinal epithelial barrier dysfunction by inhibiting myosin light chain kinase production
Cell. Physiol. Biochem.
41
1924-1934
2017
Homo sapiens
Manually annotated by BRENDA team
Burbank, M.G.; Burban, A.; Sharanek, A.; Weaver, R.J.; Guguen-Guillouzo, C.; Guillouzo, A.
Early alterations of bile canaliculi dynamics and the Rho kinase/myosin light chain kinase pathway are characteristics of drug-induced intrahepatic cholestasis
Drug Metab. Dispos.
44
1780-1793
2016
Homo sapiens
Manually annotated by BRENDA team
Pai, Y.C.; Weng, L.T.; Wei, S.C.; Wu, L.L.; Shih, D.Q.; Targan, S.R.; Turner, J.R.; Yu, L.C.
Gut microbial transcytosis induced by tumor necrosis factor-like 1A-dependent activation of a myosin light chain kinase splice variant contributes to inflammatory bowel disease
J. Crohns Colitis
2021
258-272
2020
Homo sapiens (Q15746), Homo sapiens (Q9H1R3), Homo sapiens
Manually annotated by BRENDA team
Kamikubo, K.; Tsukamoto, O.; Uyama-Saito, Y.; Oya, R.; Tsubota, T.; Fujino, N.; Asano, Y.; Kato, H.; Matsuoka, K.; Takashima, S.
Non-radioactive in vitro cardiac myosin light chain kinase assays
J. Vis. Exp.
160
e61168
2020
Homo sapiens
Manually annotated by BRENDA team