Information on EC 2.7.11.18 - myosin-light-chain kinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.11.18
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RECOMMENDED NAME
GeneOntology No.
myosin-light-chain kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + [myosin light chain] = ADP + [myosin light chain] phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:[myosin light chain] O-phosphotransferase
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
51845-53-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
strain Ax-3
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
horseshoe crab
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
C57BL/6 mice
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Manually annotated by BRENDA team
two isoforms X1 and isoform X2
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
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structure-function analysis of the non-muscle myosin light chain kinase (nmMLCK) isoform by NMR spectroscopy and molecular modeling, overview. The two major nmMLCK splice variants, nmMLCK1 (about 211 kDa) and nmMLCK2 (about 203 kDa), differ by 69 amino acids within exon 11 (partially deleted in nmMLCK2), a stretch that contains the highly phosphorylated Y464 and Y471, suggesting that nmMLCK splice variants are differentially regulated by Tyr phosphorylation. Potential involvement of S147P and P21H SNP sites in nmMLCK1 in phosphorylation-dependent binding of 14-3-3 proteins
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 20kDa myosin light chain
ADP + 20kDa myosin light chain phosphate
show the reaction diagram
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phosphorylation occurs at Ser-19 and Thr-18
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?
ATP + BpaKKRAARATSNVFA
ADP + ?
show the reaction diagram
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Bpa is the photoreactive amino acid p-benzoylphenylalanine
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?
ATP + H-KKRAARATSNVFA-NH2
ADP + ?
show the reaction diagram
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peptide substrate
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?
ATP + kemptamide
ADP + ?
show the reaction diagram
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?
ATP + kemptamide
ADP + kemptamide phosphate
show the reaction diagram
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i.e. peptide KKRPQRATSNVFS-NH2, activated enzyme
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?
ATP + KKRAARATSNVFA
ADP + ?
show the reaction diagram
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?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
ATP + Lys-Lys-Arg-Ala-Ala-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ala
ADP + ?
show the reaction diagram
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?
ATP + Lys-Lys-Arg-Pro-Gln-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ser
ADP + ?
show the reaction diagram
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?
ATP + myosin II
ADP + myosin II phosphate
show the reaction diagram
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from Dictyostelium discoideum, activated enzyme
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?
ATP + myosin IIB
ADP + phosphorylated myosin IIB
show the reaction diagram
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?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
ATP + myosin light chain 2
ADP + myosin light chain 2 phosphate
show the reaction diagram
ATP + myosin regulatory light chain
?
show the reaction diagram
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?
ATP + myosin regulatory light chain
ADP + myosin regulatory light chain phosphate
show the reaction diagram
ATP + myosin regulatory light chain
ADP + phosphorylated myosin regulatory light chain
show the reaction diagram
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?
ATP + myosin-II regulatory light chain
ADP + myosin-II regulatory light chain phosphate
show the reaction diagram
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?
ATP + obscurin
ADP + obscurin phosphate
show the reaction diagram
ATP + protein
ADP + phosphoprotein
show the reaction diagram
ATP + telokin
ADP + ?
show the reaction diagram
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telokin may modulate enzyme activity in vivo
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?
ATP + TRPC5 channel
ADP + phosphorylated TRPC5 channel
show the reaction diagram
Ca2+/calmodulin-dependent myosin light chain kinase is essential for activation of plasma membrane TRPC5 channels, responsible for Ca2+ influx, from Drosophila melanogaster expressed in HEK-293 cells, phosphorylation sites are Ser698, Ser775, and Thr700, mutational analysis
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?
ATP + [myosin II light chain]
ADP + [myosin II lihght chain] phosphate
show the reaction diagram
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from Dictyostelium discoideum, activated enzyme
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?
ATP + [myosin II regulatory light chain]
ADP + [myosin II regulatory light chain] phosphate
show the reaction diagram
ATP + [myosin light chain 2a]
ADP + [myosin light chain 2a] phosphate
show the reaction diagram
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
ATP + [non-muscle heavy meromyosin IIB]
ADP + [non-muscle heavy meromyosin IIB] phosphate
show the reaction diagram
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?
ATP + [non-muscle regulatory light chain]
ADP + [non-muscle regulatory light chain] phosphate
show the reaction diagram
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?
ATP + [smooth muscle heavy meromyosin]
ADP + [smooth muscle heavy meromyosin] phosphate
show the reaction diagram
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?
ATP + [smooth muscle regulatory light chain]
ADP + [smooth muscle regulatory light chain] phosphate
show the reaction diagram
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?
additional information
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
ATP + myosin IIB
ADP + phosphorylated myosin IIB
show the reaction diagram
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?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
ATP + myosin light chain 2
ADP + myosin light chain 2 phosphate
show the reaction diagram
Q6PDN3
MLCK is involved in sarcomere organization, cardiac MLCK promotes sarcomere organization and increases cardiomyocyte contractility, overview
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?
ATP + myosin regulatory light chain
ADP + phosphorylated myosin regulatory light chain
show the reaction diagram
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?
ATP + obscurin
ADP + obscurin phosphate
show the reaction diagram
ATP + [myosin II regulatory light chain]
ADP + [myosin II regulatory light chain] phosphate
show the reaction diagram
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involved in many cellular cytoskeletal functions
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?
ATP + [myosin light chain 2a]
ADP + [myosin light chain 2a] phosphate
show the reaction diagram
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MLC phosphorylation plays a regulatory role in inotropic response to alpha1-adrenergic stimulation in the heart, enzyme inhibition causes heart failure in ventricular trabeculae
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ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin