Information on EC 2.7.11.18 - myosin-light-chain kinase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
2.7.11.18
-
RECOMMENDED NAME
GeneOntology No.
myosin-light-chain kinase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + [myosin light chain] = ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:[myosin light chain] O-phosphotransferase
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+-calmodulin-dependent myosin light chain kinase
-
calcium/calmodulin-dependent myosin light chain kinase
-
-
-
-
cardiac-MLCK
-
-
cardiac-specific myosin light chain kinase
-
cMLKCk
-
cardiac myosin light chain kinase
DNA-dependent protein kinase catalytic subunit
-
endothelial cell myosin light chain kinase
-
-
endothelial MLCK
-
-
endothelial myosin light chain kinase
-
-
kinase, myosin light-chain (phosphorylating)
-
-
-
-
long chain myosin light chain kinase
-
-
long myosin light chain kinase
-
-
MLC kinase
-
-
MLC kinase
Mus musculus C57BL/6
-
-
-
MLC kinase
-
-
MLC-kinase
-
-
MLCK
-
-
MLCK
-
-
MLCK
-
-
MLCK
Mus musculus C57BL/6
-
-
-
MLCK-210
-
-
MLCK-210
-
-
MYLK
-
-
myosin kinase
-
-
-
-
myosin light chain kinase
-
-
myosin light chain kinase
-
-
myosin light chain kinase
-
-
myosin light chain kinase
-
myosin light chain kinase
Dictyostelium discoideum Ax-3
-
-
myosin light chain kinase
-
-
myosin light chain kinase
-
myosin light chain kinase
-
myosin light chain kinase
-
-
myosin light chain kinase
-
myosin light chain kinase
Mus musculus C57BL/6
-
-
-
myosin light chain kinase
-
-
myosin light chain kinase
-
myosin light chain kinase
-
myosin light chain kinase
-
myosin light chain kinase 2, skeletal/cardiac muscle
-
myosin light chain kinase A
-
-
myosin light chain kinase, skeletal muscle
-
myosin light chain kinase, smooth muscle
-
myosin light chain kinase, smooth muscle
-
myosin light chain kinase, smooth muscle and non-muscle isozymes
-
myosin light chain kinase, smooth muscle and non-muscle isozymes
-
myosin light chain protein kinase
-
-
-
-
myosin light-chain kinase
-
-
-
-
myosin light-chain kinase
-
-
myosin light-chain kinase
-
myosin light-chain kinase
-
-
myosin light-chain kinase
-
-
myosin light-chain kinase
-
myosin light-chain kinase
-
-
myosine light chain kinase
-
-
myosine light chain kinase
-
-
nmMLCK1
-
non-muscle myosin light chain kinase variant
nmMLCK2
-
-
non-muscle myosin light chain kinase
-
-
non-muscle myosin light chain kinase
-
-
obscurin-associated kinase
-
-
obscurin-MLCK-like kinase
-
-
short myosin light chain kinase
-
-
skMLCK
-
skeletal muscle myosin light chain kinase
smMLCK
-
-
smMLCK
-
smooth muscle myosin light chain kinase
smooth muscle myosin light chain kinase
-
-
smooth-muscle myosin light chain kinase
-
-
smooth-muscle-myosin-light-chain kinase
-
-
-
-
stretchin-MLCK
-
-
Twitchin kinase
-
-
additional information
-
-
-
additional information
cf. EC 2.7.11.17
additional information
-
cf. EC 2.7.11.17
CAS REGISTRY NUMBER
COMMENTARY
51845-53-5
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
; isolation of cDNA from bovine stomach
SwissProt
Manually annotated by BRENDA team
steer
-
-
Manually annotated by BRENDA team
cardiac/ventricular isozyme cardiac-MLCK encoded by gene MLCK3, and smooth muscle isozyme encoded by gene MLCK2
-
-
Manually annotated by BRENDA team
; strain Ax-3
SwissProt
Manually annotated by BRENDA team
Dictyostelium discoideum Ax-3
strain Ax-3
SwissProt
Manually annotated by BRENDA team
; mutant analyses
SwissProt
Manually annotated by BRENDA team
; regulatory and structural motifs
SwissProt
Manually annotated by BRENDA team
2 isozymes encoded by a single gene and translated from 2 different splicing variants
-
-
Manually annotated by BRENDA team
cardiac/ventricular isozyme cardiac-MLCK encoded by gene MLCK3, and smooth muscle isozyme encoded by gene MLCK2
-
-
Manually annotated by BRENDA team
endothelial isozyme EC MLCK exists in different splice variants
-
-
Manually annotated by BRENDA team
gene MYLK encoding several isozymes
-
-
Manually annotated by BRENDA team
skeletal/cardiac muscle isozyme MYLK2
SwissProt
Manually annotated by BRENDA team
smooth muscle isozyme MYLK
SwissProt
Manually annotated by BRENDA team
splicing variant 1, i.e. isozyme MLCK1; 2 endothelial isozymes MLCK1 and MLCK2; splicing variant 2, i.e. isozyme MLCK2; 2 endothelial isozymes MLCK1 and MLCK2
SwissProt
Manually annotated by BRENDA team
horseshoe crab
-
-
Manually annotated by BRENDA team
2 isozymes encoded by a single gene and translated from 2 different splicing variants
-
-
Manually annotated by BRENDA team
C57BL/6 mice
-
-
Manually annotated by BRENDA team
female Harlan Sprague-Dawley mice
-
-
Manually annotated by BRENDA team
gene mylk1, encoding two isozymes: the endothelial, long or nonmuscle 220-kDa MLCK and a short, smooth muscle 130-kDa MLCK
-
-
Manually annotated by BRENDA team
male strain C57-black6/SV mice, isozyme MLCK-210
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6
C57BL/6 mice
-
-
Manually annotated by BRENDA team
domain characterization of the skeletal muscle enzyme
SwissProt
Manually annotated by BRENDA team
new Zealand white
-
-
Manually annotated by BRENDA team
New Zealand white rabbits
-
-
Manually annotated by BRENDA team
short enzyme form
-
-
Manually annotated by BRENDA team
pregnant and non-pregnant
-
-
Manually annotated by BRENDA team
; tissue distribution
SwissProt
Manually annotated by BRENDA team
adult male Wistar rats
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
MLCK-deficient mice lack bronchial constrictions in normal and asthmatic airways, while the asthmatic inflammation response is not affected
physiological function
-
MLCK phosphorylates myosin regulatory light chain and controls muscle contraction of skeletal muscle, smooth muscle, and cardiac muscle
physiological function
-
myosin light chain kinase acts as a central participant in the contractile signaling module of tonic smooth muscle
physiological function
-
myosin light chain kinase mediates transcellular intravasation of breast cancer cells through the underlying endothelial cells
physiological function
-
phosphorylation of myosin light chains by myosin light chain kinase activates endothelial contractile elements and results in a rearrangement of the cytoskeleton
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 20kDa myosin light chain
ADP + 20kDa myosin light chain phosphate
show the reaction diagram
-
phosphorylation occurs at Ser-19 and Thr-18
-
?
ATP + BpaKKRAARATSNVFA
ADP + ?
show the reaction diagram
-
Bpa is the photoreactive amino acid p-benzoylphenylalanine
-
?
ATP + H-KKRAARATSNVFA-NH2
ADP + ?
show the reaction diagram
-
peptide substrate
-
?
ATP + kemptamide
ADP + ?
show the reaction diagram
-
-
-
?
ATP + kemptamide
ADP + kemptamide phosphate
show the reaction diagram
-
i.e. peptide KKRPQRATSNVFS-NH2, activated enzyme
-
?
ATP + KKRAARATSNVFA
ADP + ?
show the reaction diagram
-
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
cardiac-MLCK is essential for normal cardiac development and function in zebrafish embryos, deletion of the gene encoding cardiac-MLCK is lethal for embryos
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
causes endothelial contraction
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
cytokine-induced epithelial barrier dysfunction can be mediated by increased MLCK expression and subsequent myosin II regulatory light chain phosphorylation, the enzyme is involved in the inflammatory bowel disease
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
MLC phosphorylation increases muscle contraction and plays a prominent role in skeletal muscle force potentiation of fast-twitch type IIb but not type I or IIa fibers, overview
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
MLCK plays a key role in the control of MLC-phosphorylation status, and it modulates barrier function through its regulation of intracellular contractile machinery, overview, endothelial mechanism of MLC-dependent barrier injury in burns, overview
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
myosin II activation is essential for stress fiber and focal adhesion formation, and is implicated in integrin-mediated signaling events
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
regulatory myosin chain, in thick filaments, isozyme L-MLCK plays a role in cytoskeleton organization
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
roles of MLCK and ROCK on myosin II activation, overview, a global, MLCK-dependent increase in myosin II cortical contractility accompanies the metaphase-anaphase transition in sea urchin eggs, overview
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
the enzyme is important in regulation of shape, adhesion and migration of fibrosarcoma cells, overview
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
phosphorylation at Ser19
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
regulatory myosin chain
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
regulatory myosin chain, phosphorylation at Ser18 and Thr19 on the N-terminus
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
regulatory myosin chain, phosphorylation at Ser18 and Thr19 on the N-terminus
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
regulatory myosin light chain
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
the cardiac-specific substrate cardiac-MLCKis phosphorylated by the cardiac-specific isozyme MLC2v
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
the cardiac-specific substrate MLC2v is phosphorylated by the cardiac-specific isozyme cardiac-MLCK
-
?
ATP + Lys-Lys-Arg-Ala-Ala-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ala
ADP + ?
show the reaction diagram
-
-
-
?
ATP + Lys-Lys-Arg-Pro-Gln-Arg-Ala-Thr-Ser-Asn-Val-Phe-Ser
ADP + ?
show the reaction diagram
-
-
-
?
ATP + myosin II
ADP + myosin II phosphate
show the reaction diagram
-
from Dictyostelium discoideum, activated enzyme
-
?
ATP + myosin IIB
ADP + phosphorylated myosin IIB
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
phosphorylation of myosin light chains by myosin light chain kinase is a key event in agonist-mediated endothelial cell gap formation and vascular permeability
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
activated MLCK then phosphorylates the regulatory myosin light chains, triggering cross-bridge cycling and contraction, in aortic rings from mice in which alpha1, the major catalytic subunit isoform in arterial smooth muscle, is deleted, KCl- or phenylephrine-induced contraction is increased, overview
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
Dictyostelium discoideum Ax-3
the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrates are myosin heavy chain and phosvitin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrates are myosin heavy chain and phosvitin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrates are myosin heavy chain and phosvitin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrates are myosin heavy chain and phosvitin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrates are myosin heavy chain and phosvitin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrates are myosin heavy chain and phosvitin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
no substrates are myosin heavy chain and phosvitin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is protamine
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is protamine
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of cardiac muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrates are actin and tropomyosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
transfers the gamma-phosphate of ATP to a Ser-residue of myosin light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
no substrate is histone 2-A
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
ITP, GTP, CTP or UTP cannot replace ATP
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of non-muscle myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
not specific for 22 kDa and 15 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
not specific for 22 kDa and 15 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
not specific for 22 kDa and 15 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
no substrate is casein
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrates are 15 kDa and 22 kDa light-chain or heavy-chain fractions of myosin from white skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
phosphorylation sites: Ser-residues in smooth and skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of adrenal medullary myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2 b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone 2 b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is troponin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is troponin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is troponin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is troponin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone H1
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for regulatory or P-light-chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for 18 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for 18 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for 18 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for 18 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for 18 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
highly specific for 18 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 18.5 kDa Dictyostelium or 19 kDa skeletal muscle light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is synapsin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is synapsin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
1 mol phosphate per mol light chain in skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
1 mol phosphate per mol light chain in skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
1 mol phosphate per mol light chain in skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
1 mol phosphate per mol light chain in skeletal muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase kinase
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase kinase
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase kinase
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase kinase
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase kinase
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase kinase
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is molluscan adductor myosin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
specific for 20 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
phosphorylation sites: Ser-19 and Thr-18 in smooth muscle myosin light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrates are myelin basic protein, glycogen synthase, tubulin, microtubule-associated protein 2, kemptide and peptide pp60src
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
not 16 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
not 16 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
not 16 kDa light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone V-S
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone V-S
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone V-S
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone V-S
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
acceptor substrates are myosin light chains of Ml3-myosin rabbit muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
no substrate is phosphorylase b
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
no substrate is histone III-S from thymus
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
kinase from skeletal muscle with broader specificity than smooth muscle kinase
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
involved in myosin phosphorylation and enzyme secretion
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
obligatory step in development of active tension in smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
involved in muscle contractility and motility of non-muscle cells
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
involved in regulation of actin-myosin contractile activity in adrenal medulla
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
event in initiation of smooth-muscle contraction
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
event in initiation of smooth-muscle contraction
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
inhibition of actin-myosin ineraction
-
-
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
inhibition of actin-myosin ineraction
-
-
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
phosphorylation at Ser19
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
activated MLCK then phosphorylates the regulatory myosin light chains, triggering cross-bridge cycling and contraction, myosin light chain phosphorylation is increased under these conditions, suggesting that contraction would be potentiated by ablation of AMPK
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
activated MLCK then phosphorylates the regulatory myosin light chains, triggering cross-bridge cycling and contraction, myosin light chain phosphorylation is increased under these conditions, suggesting that contraction would be potentiated by ablation of AMPK
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
in airway smooth muscles, kinase/phosphatase-dependent phosphorylation and dephosphorylation of the myosin light chain is an important step in calcium signalling pathway from the variation of Ca2+ concentration in cytosol to the force development
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
MLCK plays a critically important role in regulation of contraction in both smooth muscle and non-muscle cells, MLCK is both necessary and sufficient to initiate smooth muscle contraction
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
MLCK plays a crucial role in the cell migration and tumor metastasis
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
the MLCK family plays an essential role in regulation of actin/myosin organization, sarcomere assembly and cytoskeletal dynamics
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
the enzyme has effects on the actin-myosin interaction in vitro, binding of full-length and fragmented enzyme, overview. The Asp-Phe-Arg-X-X-Leu motif plays a major role in the binding of MLCK to myofibrils. Regulation of actinmyosin interaction by MLCK through its non-kinase activity, overview
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
the enzyme has effects on the actin-myosin interaction in vitro, regulation of actin-myosin interaction by MLCK through its non-kinase activity, overview
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
20 kDa regulatory light chain of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
20 kDa regulatory light chain of smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
myosin light chain is phosphorylated at Thr 18 and Ser 19 by myosin light chain kinase
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
Dictyostelium discoideum Ax-3
no substrates are myosin heavy chain and phosvitin
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
Mus musculus C57BL/6
-
-
-
?
ATP + myosin light chain 2
ADP + myosin light chain 2 phosphate
show the reaction diagram
MLCK is involved in sarcomere organization, cardiac MLCK promotes sarcomere organization and increases cardiomyocyte contractility, overview
-
?
ATP + myosin regulatory light chain
?
show the reaction diagram
-
-
-
-
?
ATP + myosin regulatory light chain
ADP + myosin regulatory light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin regulatory light chain
ADP + myosin regulatory light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin regulatory light chain
ADP + phosphorylated myosin regulatory light chain
show the reaction diagram
-
-
-
?
ATP + myosin-II regulatory light chain
ADP + myosin-II regulatory light chain phosphate
show the reaction diagram
-
-
-
?
ATP + obscurin
ADP + obscurin phosphate
show the reaction diagram
-
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
Dictyostelium discoideum, Dictyostelium discoideum Ax-3
intramolecular autophosphorylation activates the enzyme
-
?
ATP + telokin
ADP + ?
show the reaction diagram
-
telokin may modulate enzyme activity in vivo
-
?
ATP + TRPC5 channel
ADP + phosphorylated TRPC5 channel
show the reaction diagram
Ca2+/calmodulin-dependent myosin light chain kinase is essential for activation of plasma membrane TRPC5 channels, responsible for Ca2+ influx, from Drosophila melanogaster expressed in HEK-293 cells, phosphorylation sites are Ser698, Ser775, and Thr700, mutational analysis
-
?
ATP + [myosin II light chain]
ADP + [myosin II lihght chain] phosphate
show the reaction diagram
-
from Dictyostelium discoideum, activated enzyme
-
?
ATP + [myosin II regulatory light chain]
ADP + [myosin II regulatory light chain] phosphate
show the reaction diagram
-
involved in many cellular cytoskeletal functions
-
?
ATP + [myosin light chain 2a]
ADP + [myosin light chain 2a] phosphate
show the reaction diagram
-
MLC phosphorylation plays a regulatory role in inotropic response to alpha1-adrenergic stimulation in the heart, enzyme inhibition causes heart failure in ventricular trabeculae
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
activation of the enzyme is a key regulatory step in the modulation of endothelial permeability, central to parthogenesis of inflammatory lung disease is disruption of the endothelial barrier function
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
enzyme is regulating cytoskeletal rearrangement and cell motility
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
the endothelial isozyme is a mutifunctional contractile effector involved in vascular barrier regulation, leukocyte diapedesis, apoptosis, and angiogenesis
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
the multifunctional regulatory enzyme stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain, kinetics, overview
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
the non-muscle myosin light chain kinase is involved in regulation of hepatic functions in response to intra- and extracellular signals
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
activated phosphorylated enzyme
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
activated phosphorylated enzyme
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
activated phosphorylated enzyme
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
activated phosphorylated enzyme
-
?
additional information
?
-
-
substrate specificity
-
-
-
additional information
?
-
-
the enzyme possesses ATPase activity
-
-
-
additional information
?
-
-
skeletal, gizzard smooth and cardiac enzymes perform intramolecular autophosphorylation in the absence of acceptor substrate
-
-
-
additional information
?
-
skeletal, gizzard smooth and cardiac enzymes perform intramolecular autophosphorylation in the absence of acceptor substrate
-
-
-
additional information
?
-
-
no intramolecular autophosphorylation in the absence of acceptor substrate
-
-
-
additional information
?
-
-
no intramolecular autophosphorylation in the absence of acceptor substrate
-
-
-
additional information
?
-
the differentiation-dependent enzyme splice variant MLCK1 regulates epithelial tight junction permeability, the endothelial isozyme is responsible for Na+-glucose cotransport-induced tight junction regulation
-
-
-
additional information
?
-
-
the enzyme activity is cell cycle regulated with a decrease in activity during mitosis due to phosphorylation, inhibition of enzyme phosphorylation during mitosis leads to microtubule defects, overview
-
-
-
additional information
?
-
-
the enzyme interacts with macrophage migration inhibition factor MIF in endothelium which may be important for the regulation of both non-muscle cytoskeletal dynamics as well as pathobiological vascular events involving the enzyme
-
-
-
additional information
?
-
-
the enzyme is a potential cytoskeleton integrator through its unique N-terminal domain, the enzyme binds tubulin and actin, and is involved in microtubule/microfilament association
-
-
-
additional information
?
-
-
the enzyme is involved, probably via microfilament assembly, in sperm chromatin-induced cortical reorganization during oocyte maturation to a polarized egg
-
-
-
additional information
?
-
-
the enzyme mediates eosinophil chemotaxis in a mitogen-activated protein kinase-dependent manner
-
-
-
additional information
?
-
-
the enzyme plays an important role in TNFalpha-dependent induction of NFkappaB activity, mechanism
-
-
-
additional information
?
-
-
substrate specificity and binding structure, the enzyme depends on basic residues for substrate recognition, autoregulation by a pseudosubstrate mechanism, overview
-
-
-
additional information
?
-
-
Human T cell leukemia virus 1-infected lymphocytes can cause a breakdown of the blood-brain barrier inducing an increase in paracellular endothelial permeability and transcellular migration, this disruption is associated with tight junction disorganization between endothelial cells and altered tight junction proteinexpression involving the enzyme MLCK, enzyme inhibition prevents the changes in endothelial cells, overview
-
-
-
additional information
?
-
-
increased MLCK activity accompanies increases in MLC expression, overview
-
-
-
additional information
?
-
-
infection of brain endothelial cells with Haemophilus somnus causes alterations in brain endothelial cell and blood-brain barrier integrity and causes inflammation involving the enzyme, which acts in rapid cytoskeletal rearrangement, overview
-
-
-
additional information
?
-
-
inhibition of MLCK results in spherical cells and marked impairment of adhesion and migration of fibrosarcoma cells, overview
-
-
-
additional information
?
-
-
inhibition of the enzyme retards the growth of mammary cancer cells
-
-
-
additional information
?
-
-
inhibition of the enzyme retards the growth of prostate cancer cells
-
-
-
additional information
?
-
-
MLCK is probably involved in development of diabetic nephropathy, overview
-
-
-
additional information
?
-
-
regulation of the MLCK promoters and gene expression involving myocardin, model, overview
-
-
-
additional information
?
-
-
the enzyme binds to thin filaments with high affinity, myofilament binding by Ig-like modules is blocked by disassembling of the filaments, overview
-
-
-
additional information
?
-
-
the enzyme is a key regulator of various forms of cell motility involving actin and myosin II
-
-
-
additional information
?
-
-
the enzyme is not a regulator of synaptic vesicle trafficking during repetitive exocytosis in cultured hippocampal neurons, overview
-
-
-
additional information
?
-
-
the enzyme negatively regulates synaptic plasticity and fear learning in the lateral nucleus of the amygdala, the enzyme is involved in memory aquisitoion, rather than in posttraining consolidation of memory, the enzyme may help to prevent aquisition of irrelevant fears, thus enzyme deficiency or defects can lead to pathological fear learning, overview
-
-
-
additional information
?
-
-
the myosin light chain kinase plays a role in the regulation of epithelial cell survival, and in the generation of pro-survival signals in both untransformed and transformed epithelial cells, apoptosis following inhibition of myosin II activation by MLCK is probably meditated through the death receptor pathway, overview
-
-
-
additional information
?
-
-
ventricular/cardiac muscle isozyme cardiac-MLCK, which is involved in the development of human cardiomyopathy, is an important structural protein that affects physiologic cardiac sarcomere formation and heart development, the cardiac MLCK regulates sarcomere assembly in the vertebrate heart, overview
-
-
-
additional information
?
-
MLCK in the plasma membrane distribution of TRPC5 channels, overview
-
-
-
additional information
?
-
-
actin-myosin interaction of smooth muscle could be activated by the non-kinase activity of MLCK, a mechanism that is quite independent of MLC20 phosphorylation, role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, overview
-
-
-
additional information
?
-
-
actin-myosin interaction of smooth muscle could be activated by the non-kinase activity of MLCK, a mechanism that is quite independent of MLC20 phosphorylation, role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, overview. MLCK phosphorylated at Ser828 stimulates the ATPase activity of unphosphorylated myosin more effectively than the control MLCK
-
-
-
additional information
?
-
-
cytochalasin-induced increases in paracellular permeability are also mediated by MLC in Caco-2 monolayers
-
-
-
additional information
?
-
-
MLCK is a substrate of AMP-activated protein kinase, AMPK
-
-
-
additional information
?
-
MLCK is a substrate of AMP-activated protein kinase, AMPK
-
-
-
additional information
?
-
-
myosin light chain kinase is central to smooth muscle contraction and required for gastrointestinal motility in mice, overview
-
-
-
additional information
?
-
-
myosin light-chain kinase contributes to the proliferation and migration of breast cancer cells through cross-talk with activated ERK1/2, overview
-
-
-
additional information
?
-
-
PKA regulates beta2-integrin avidity activation via a MLCK-dependent pathway, overview
-
-
-
additional information
?
-
postnatal decreases in vascular reactivity involve decreases in the thick filament component of myofilament calcium sensitivity, which is measured as the relationship between cytosolic calcium concentration and myosin light chain phosphorylation, relations between myosin light chain phosphorylation and contractile force, overview
-
-
-
additional information
?
-
-
the enzyme is involved in inflammation response, including apoptosis, vascular permeability, and leukocyte diapedesis. Single nucleotide polymorphisms are involved in the risk of sepsis-related acute lung injury and asthma
-
-
-
additional information
?
-
the cardiac-specific isozyme probably performs also autophosphorylation
-
-
-
additional information
?
-
-
the enzyme also exerts non-kinase activity on myosin motor and myosin ATPase activities
-
-
-
additional information
?
-
the enzyme also exerts non-kinase activity on myosin motor and myosin ATPase activities
-
-
-
additional information
?
-
Dictyostelium discoideum Ax-3
skeletal, gizzard smooth and cardiac enzymes perform intramolecular autophosphorylation in the absence of acceptor substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
-
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
cardiac-MLCK is essential for normal cardiac development and function in zebrafish embryos, deletion of the gene encoding cardiac-MLCK is lethal for embryos
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
causes endothelial contraction
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
cytokine-induced epithelial barrier dysfunction can be mediated by increased MLCK expression and subsequent myosin II regulatory light chain phosphorylation, the enzyme is involved in the inflammatory bowel disease
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
MLC phosphorylation increases muscle contraction and plays a prominent role in skeletal muscle force potentiation of fast-twitch type IIb but not type I or IIa fibers, overview
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
MLCK plays a key role in the control of MLC-phosphorylation status, and it modulates barrier function through its regulation of intracellular contractile machinery, overview, endothelial mechanism of MLC-dependent barrier injury in burns, overview
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
myosin II activation is essential for stress fiber and focal adhesion formation, and is implicated in integrin-mediated signaling events
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
regulatory myosin chain, in thick filaments, isozyme L-MLCK plays a role in cytoskeleton organization
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
roles of MLCK and ROCK on myosin II activation, overview, a global, MLCK-dependent increase in myosin II cortical contractility accompanies the metaphase-anaphase transition in sea urchin eggs, overview
-
?
ATP + light chain myosin II
ADP + phosphorylated light chain myosin II
show the reaction diagram
-
the enzyme is important in regulation of shape, adhesion and migration of fibrosarcoma cells, overview
-
?
ATP + myosin IIB
ADP + phosphorylated myosin IIB
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
P11799
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
P11799
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
P11799
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
P20689
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
P25323
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
P29294
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
Q28824
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
phosphorylation of myosin light chains by myosin light chain kinase is a key event in agonist-mediated endothelial cell gap formation and vascular permeability
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
P25323
the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
-
activated MLCK then phosphorylates the regulatory myosin light chains, triggering cross-bridge cycling and contraction, in aortic rings from mice in which alpha1, the major catalytic subunit isoform in arterial smooth muscle, is deleted, KCl- or phenylephrine-induced contraction is increased, overview
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
P25323
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
involved in myosin phosphorylation and enzyme secretion
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
obligatory step in development of active tension in smooth muscle
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
involved in muscle contractility and motility of non-muscle cells
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
involved in regulation of actin-myosin contractile activity in adrenal medulla
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
event in initiation of smooth-muscle contraction
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
P29294
event in initiation of smooth-muscle contraction
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
inhibition of actin-myosin ineraction
-
-
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
inhibition of actin-myosin ineraction
-
-
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
activated MLCK then phosphorylates the regulatory myosin light chains, triggering cross-bridge cycling and contraction, myosin light chain phosphorylation is increased under these conditions, suggesting that contraction would be potentiated by ablation of AMPK
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
P20689
activated MLCK then phosphorylates the regulatory myosin light chains, triggering cross-bridge cycling and contraction, myosin light chain phosphorylation is increased under these conditions, suggesting that contraction would be potentiated by ablation of AMPK
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
in airway smooth muscles, kinase/phosphatase-dependent phosphorylation and dephosphorylation of the myosin light chain is an important step in calcium signalling pathway from the variation of Ca2+ concentration in cytosol to the force development
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
O02827
MLCK plays a critically important role in regulation of contraction in both smooth muscle and non-muscle cells, MLCK is both necessary and sufficient to initiate smooth muscle contraction
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
MLCK plays a crucial role in the cell migration and tumor metastasis
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
the MLCK family plays an essential role in regulation of actin/myosin organization, sarcomere assembly and cytoskeletal dynamics
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
Dictyostelium discoideum Ax-3
P25323
the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
Dictyostelium discoideum Ax-3
P25323
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
Mus musculus C57BL/6
-
-
-
?
ATP + myosin light chain 2
ADP + myosin light chain 2 phosphate
show the reaction diagram
Q6PDN3
MLCK is involved in sarcomere organization, cardiac MLCK promotes sarcomere organization and increases cardiomyocyte contractility, overview
-
?
ATP + myosin regulatory light chain
ADP + phosphorylated myosin regulatory light chain
show the reaction diagram
-
-
-
?
ATP + obscurin
ADP + obscurin phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin II regulatory light chain]
ADP + [myosin II regulatory light chain] phosphate
show the reaction diagram
-
involved in many cellular cytoskeletal functions
-
?
ATP + [myosin light chain 2a]
ADP + [myosin light chain 2a] phosphate
show the reaction diagram
-
MLC phosphorylation plays a regulatory role in inotropic response to alpha1-adrenergic stimulation in the heart, enzyme inhibition causes heart failure in ventricular trabeculae
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
-
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
activation of the enzyme is a key regulatory step in the modulation of endothelial permeability, central to parthogenesis of inflammatory lung disease is disruption of the endothelial barrier function
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
enzyme is regulating cytoskeletal rearrangement and cell motility
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
the endothelial isozyme is a mutifunctional contractile effector involved in vascular barrier regulation, leukocyte diapedesis, apoptosis, and angiogenesis
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
the multifunctional regulatory enzyme stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain, kinetics, overview
-
?
ATP + [myosin light chain]
ADP + [myosin light chain] phosphate
show the reaction diagram
-
the non-muscle myosin light chain kinase is involved in regulation of hepatic functions in response to intra- and extracellular signals
-
?
additional information
?
-
Q15746
the differentiation-dependent enzyme splice variant MLCK1 regulates epithelial tight junction permeability, the endothelial isozyme is responsible for Na+-glucose cotransport-induced tight junction regulation
-
-
-
additional information
?
-
-
the enzyme activity is cell cycle regulated with a decrease in activity during mitosis due to phosphorylation, inhibition of enzyme phosphorylation during mitosis leads to microtubule defects, overview
-
-
-
additional information
?
-
-
the enzyme interacts with macrophage migration inhibition factor MIF in endothelium which may be important for the regulation of both non-muscle cytoskeletal dynamics as well as pathobiological vascular events involving the enzyme
-
-
-
additional information
?
-
-
the enzyme is a potential cytoskeleton integrator through its unique N-terminal domain, the enzyme binds tubulin and actin, and is involved in microtubule/microfilament association
-
-
-
additional information
?
-
-
the enzyme is involved, probably via microfilament assembly, in sperm chromatin-induced cortical reorganization during oocyte maturation to a polarized egg
-
-
-
additional information
?
-
-
the enzyme mediates eosinophil chemotaxis in a mitogen-activated protein kinase-dependent manner
-
-
-
additional information
?
-
-
the enzyme plays an important role in TNFalpha-dependent induction of NFkappaB activity, mechanism
-
-
-
additional information
?
-
-
Human T cell leukemia virus 1-infected lymphocytes can cause a breakdown of the blood-brain barrier inducing an increase in paracellular endothelial permeability and transcellular migration, this disruption is associated with tight junction disorganization between endothelial cells and altered tight junction proteinexpression involving the enzyme MLCK, enzyme inhibition prevents the changes in endothelial cells, overview
-
-
-
additional information
?
-
-
increased MLCK activity accompanies increases in MLC expression, overview
-
-
-
additional information
?
-
-
infection of brain endothelial cells with Haemophilus somnus causes alterations in brain endothelial cell and blood-brain barrier integrity and causes inflammation involving the enzyme, which acts in rapid cytoskeletal rearrangement, overview
-
-
-
additional information
?
-
-
inhibition of MLCK results in spherical cells and marked impairment of adhesion and migration of fibrosarcoma cells, overview
-
-
-
additional information
?
-
-
inhibition of the enzyme retards the growth of mammary cancer cells
-
-
-
additional information
?
-
-
inhibition of the enzyme retards the growth of prostate cancer cells
-
-
-
additional information
?
-
-
MLCK is probably involved in development of diabetic nephropathy, overview
-
-
-
additional information
?
-
-
regulation of the MLCK promoters and gene expression involving myocardin, model, overview
-
-
-
additional information
?
-
-
the enzyme binds to thin filaments with high affinity, myofilament binding by Ig-like modules is blocked by disassembling of the filaments, overview
-
-
-
additional information
?
-
-
the enzyme is a key regulator of various forms of cell motility involving actin and myosin II
-
-
-
additional information
?
-
-
the enzyme is not a regulator of synaptic vesicle trafficking during repetitive exocytosis in cultured hippocampal neurons, overview
-
-
-
additional information
?
-
-
the enzyme negatively regulates synaptic plasticity and fear learning in the lateral nucleus of the amygdala, the enzyme is involved in memory aquisitoion, rather than in posttraining consolidation of memory, the enzyme may help to prevent aquisition of irrelevant fears, thus enzyme deficiency or defects can lead to pathological fear learning, overview
-
-
-
additional information
?
-
-
the myosin light chain kinase plays a role in the regulation of epithelial cell survival, and in the generation of pro-survival signals in both untransformed and transformed epithelial cells, apoptosis following inhibition of myosin II activation by MLCK is probably meditated through the death receptor pathway, overview
-
-
-
additional information
?
-
-
ventricular/cardiac muscle isozyme cardiac-MLCK, which is involved in the development of human cardiomyopathy, is an important structural protein that affects physiologic cardiac sarcomere formation and heart development, the cardiac MLCK regulates sarcomere assembly in the vertebrate heart, overview
-
-
-
additional information
?
-
-
actin-myosin interaction of smooth muscle could be activated by the non-kinase activity of MLCK, a mechanism that is quite independent of MLC20 phosphorylation, role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, overview
-
-
-
additional information
?
-
-
actin-myosin interaction of smooth muscle could be activated by the non-kinase activity of MLCK, a mechanism that is quite independent of MLC20 phosphorylation, role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, overview. MLCK phosphorylated at Ser828 stimulates the ATPase activity of unphosphorylated myosin more effectively than the control MLCK
-
-
-
additional information
?
-
-
cytochalasin-induced increases in paracellular permeability are also mediated by MLC in Caco-2 monolayers
-
-
-
additional information
?
-
-
MLCK is a substrate of AMP-activated protein kinase, AMPK
-
-
-
additional information
?
-
P20689
MLCK is a substrate of AMP-activated protein kinase, AMPK
-
-
-
additional information
?
-
-
myosin light chain kinase is central to smooth muscle contraction and required for gastrointestinal motility in mice, overview
-
-
-
additional information
?
-
-
myosin light-chain kinase contributes to the proliferation and migration of breast cancer cells through cross-talk with activated ERK1/2, overview
-
-
-
additional information
?
-
-
PKA regulates beta2-integrin avidity activation via a MLCK-dependent pathway, overview
-
-
-
additional information
?
-
O02827
postnatal decreases in vascular reactivity involve decreases in the thick filament component of myofilament calcium sensitivity, which is measured as the relationship between cytosolic calcium concentration and myosin light chain phosphorylation, relations between myosin light chain phosphorylation and contractile force, overview
-
-
-
additional information
?
-
-
the enzyme is involved in inflammation response, including apoptosis, vascular permeability, and leukocyte diapedesis. Single nucleotide polymorphisms are involved in the risk of sepsis-related acute lung injury and asthma
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ATP
-
-
ATP
-
-
ATP
-
residues A27, A29, A32, and A31 are involved in binding
ATP
-
ATP-binding motif X-Gly-X-Gly-X-XGly-X
Calmodulin
-
aged enzyme loses Ca2+/calmodulin sensitivity by proteolysis; requirement
Calmodulin
-
only active as ternary complex of calmodulin, Ca2+ and kinase: activation is initiated by binding of Ca2+ to calmodulin; requirement
Calmodulin
-
requirement
Calmodulin
-
lower affinity for Ca2+/caldesmon after phosphorylation by cAMP-dependent protein kinase, Ka: 0.0000006 mM; requirement
Calmodulin
-
requirement
Calmodulin
-
requirement
Calmodulin
-
requirement; trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin
Calmodulin
-
requirement
Calmodulin
-
requirement; trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin
Calmodulin
-
1:1 stoichiometric complex in the presence of Ca2+; requirement; trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin
Calmodulin
-
requirement
Calmodulin
-
1:1 stoichiometric complex in the presence of Ca2+; requirement; trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin
Calmodulin
-
1:1 stoichiometric complex in the presence of Ca2+; requirement; trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin
Calmodulin
-
1:1 stoichiometric complex in the presence of Ca2+; requirement; trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin
Calmodulin
-
1:1 stoichiometric complex in the presence of Ca2+; requirement; trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin
Calmodulin
-
1:1 stoichiometric complex in the presence of Ca2+; requirement; trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin
Calmodulin
-
1:1 stoichiometric complex in the presence of Ca2+; requirement; trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin
Calmodulin
-
calmodulin in presence of Ca2+ abolishes the inhibition of actin-myosin interaction
Calmodulin
-
optimal ratio of enzyme to calmodulin for telokin phosphorylation is 1:4
Calmodulin
-
-
Calmodulin
-
binding site I at residue 1002-1022, binding site II at residues 26-42, complex formation with Ca2+
Calmodulin
-
dependent on
Calmodulin
-
dependent on
Calmodulin
-
Calmodulin
-
-
Calmodulin
-
dependent on
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
Ca2+/calmodulin-dependent
Ca2+
calcium/calmodulin regulation; not
Ca2+
-
; Ca2+/calmodulin-dependent
Ca2+
-
effect depends on Mg2+ concentration
Ca2+
-
aged enzyme loses Ca2+/calmodulin sensitivity by proteolysis; inhibits at higher free concentrations; requirement, only in combination with calmodulin
Ca2+
-
Km-value: 0.0003
Ca2+
-
KM: 0.0025 mM
Ca2+
-
-
Ca2+
-
Ca2+ in presence of calmodulin abolishes the inhibition of actin-myosin interaction
Ca2+
-
complex formation with calmodulin
Ca2+
-
activates
Ca2+
-
dependent on
Ca2+
activates, dependent on; activates, dependent on
Ca2+
-
the enzyme is dependent on Ca2+ and calmodulin, the intracellular Ca2+ concentration has regulatory effect, overview
Ca2+
-
dependent on, antagonizes the binding to actin, Ca/CaM binds at the Ala796-Ser815 sequence to regulate the kinase activity MLCK
Ca2+
-
dependent on, antagonizes the binding to actin
Ca2+
-
dependent on
Ca2+
-
-
Ca2+
-
required
Ca2+
-
required
Ca2+
-
dependent on
Ca2+
-
MLCK is maximally activated by about 0.001 mM of Ca2+
Ca2+
activates
Ca2+
-
activates
Ca2+
-
required for activity
Ca2+
-
activates
Ca2+
-
activates
Calcium
-
calcium-binding protein
Mg2+
-
-
Mg2+
-
-
Mg2+
-
requirement, varies with ATP-concentration, MgATP2- is the active substrate
Mg2+
-
Km-value: 2 mM
Mg2+
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(+)-catechin
-
IC50: 0.44 mM
(-)-epicatechin
-
IC50: 0.32 mM
1,12-diaminododecane
-
IC50: 0.063 mM
1,4-diaminoanthraquinone
-
IC50: 0.018 mM
1-hexadecylpyridinium bromide
-
IC50: 0.049 mM
11-(3-chloro-6-iminopyridazin-1(6H)-yl)-N-(6-phenylpyridazin-3-yl)undecanamide
-
highly specific inhibitor, competitive to ATP binding
2,2'-Dihydroxychalcone
-
-
3',4',5'-tri-O-methyltricetin
-
-
3',4'-dihydroxyflavone
-
IC50: 0.262 mM
3,3',4'-trihydroxyflavone
-
IC50: 0.001 mM
5,4'-dihydroxyflavone
-
IC50: 0.024 mM
5,7-dihydroxyflavone
-
IC50: 0.043 mM
7,8,3',4'-tetrahydroxyflavone
-
IC50: 0.02 mM
7-O-methylapigenin
-
-
acylcarnitin
-
weak
AKKLSKDRMAAYMARRK
-
synthetic peptide, analog of inhibitory region of myosin light chain kinase
AKKLSKDRMKKYMA
-
synthetic peptide, analog of inhibitory region of myosin light chain kinase
AKKLSKDRMKKYMAAAA
-
synthetic peptide, analog of inhibitory region of myosin light chain kinase
AKKLSKDRMKKYMARR
-
synthetic peptide, analog of inhibitory region of myosin light chain kinase
AKKLSKDRMKKYMARRK
-
synthetic peptide, analog of inhibitory region of myosin light chain kinase
AKKLSKDRMKKYMARRKWQKTG
-
synthetic peptide, analog of inhibitory region of myosin light chain kinase
alizarin
-
IC50: 0.014 mM
alkylamine
-
long and straight chain, most effective with chain length C-13 to C-18
alkyltrimethylammonium halide
-
-
-
amiloride
-
-
anthraflavic acid
-
IC50: 0.037 mM
anthrarufin
-
-
apigenin
-
IC50: 0.023 mM
arachidonic acid
-
-
ARRKWQKTGHAVRAIGRLSS
-
-
ATP
-
free form, strong, not in the presence of excess Mg2+
blebbistatin
-
complete inhibition at 0.1 mM
Ca2+
-
at higher free concentrations, 0.4-3 mM, independent of Mg2+ or pH-value
Calmodulin-binding protein from bovine cardiac muscle
-
-
-
cAMP-dependent protein kinase
-
phosphorylates light chain myosin kinase leading to decreased affinity from calmodulin
-
cAMP-dependent protein kinase
-
phosphorylates light chain myosin kinase leading to decreased affinity from calmodulin
-
cAMP-dependent protein kinase
-
phosphorylates light chain myosin kinase leading to decreased affinity from calmodulin
-
Carbachol
-
increases Ca2+ concentration, MLCK activation and regulatory light chain phosphorylation
chalcone
-
-
chrysazine
-
IC50: 0.02 mM
chrysophanic acid
-
-
cytasters
-
low inhibition
-
D-Sphingosine
-
IC50: 0.006 mM
decylamine
-
IC50: 0.2 mM
dihydroapigenin
-
IC50: 0.17 mM
dihydrofisetin
-
IC50: 0.18 mM
dihydroluteolin
-
-
dihydroquercetin
-
IC50: 0.08 mM
dihydrosphingosine
-
erythro- and threo-dihydrosphingosine, IC50: 0.008 mM
Dimethyldioctadecylammonium bromide
-
IC50: 0.008 mM
dioctylamine
-
IC50: 0.055 mM
dodecylamine
-
IC50: 0.083 mM
dodecyltrimethylammonium bromide
-
IC50: 0.078 mM
EGTA
-
-
emodin
-
IC50: 0.008 mM
fisetin
-
IC50: 0.005 mM
galangin
-
IC50: 0.02 mM
gossypol
-
-
H1152
-
low inhibition
hesperidin
-
-
hexadecylamine
-
IC50: 0.016 mM
Hexadecyltrimethylammonium bromide
-
IC50: 0.011 mM
human arrest defective-1
-
human arrest defective-1 is a bona fide regulator of MLCK, human arrest defective-1 associates with an IgG motif of MLCK and acetylates the Lys-608 residue in this motif and by so doing deactivates MLCK
-
Increasing ionic strength
above 0.1 M KCl
-
Increasing ionic strength
-
up to 0.4 M NaCl, weak
-
Increasing ionic strength
-
-
-
isoliquiritigenin
-
-
kaempferid
-
IC50: 0.008 mM
kaempferol
-
i.e. 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-1-benzopyran-4-one, IC50: 0.00045 mM
kaempferol
-
IC50: 0.004 mM
KCl
-
increases Ca2+ concentration, MLCK activation and regulatory light chain phosphorylation
KDRMKKYMARR
-
synthetic peptide, analog of inhibitory region of myosin light chain kinase
KKRAARATS-NH2
-
i.e. KKRAARATS amide
KT5926
-
i.e. (8R,9S,11S)-(-)-9-hydroxy-9-methoxycarbonyl-8-methyl-14-n-propoxy-2,3,9,10-tetrahydro-8,11-epoxy,1H,8H,11H-2,7beta,11alpha-triazadeibenzo[a,g]cycloocta[cde]triden-1-one, specific inhibitor
lauroylcholine iodide
-
IC50: 0.12 mM
linoleic acid
-
-
LSKDRMKKYMARRKWQK
-
synthetic peptide, analog of inhibitory region of myosin light chain kinase
-
luteolin
-
IC50: 0.026 mM
merocyanine dye (C16)
-
IC50: 0.040 mM
merocyanine dye (CH3)
-
-
mitoxanthrone
-
IC50: 0.002 mM
ML-7
-
calmodulin/enzyme-specific inhibitor, inhibits cell attachment to extracellular substrate in vivo, attenuates the activation of GTP-binding protein Rac and cell migration
ML-7
-
strong specific inhibition
ML-7
-
specifically inhibits the enzyme and prevents sperm chromatin-induced cortical reorganization in eggs in vivo
ML-7
-
specific inhibitor, inhibits activation by eotaxin
ML-7
-
specific inhibitor
ML-7
-
i.e. 1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine HCl, specific inhibitor
ML-7
-
40-60% inhibition in vivo
ML-7
-
specific inhibitor of MLCK, has a chemopreventive effect in an in vitro mouse mammary organ culture model, in vivo the inhibitor retards the growth of mammary tumours, acts as an adjuvant to etoposide stimulating its ability to prevent growth of established tomours, overview
ML-7
-
specific inhibitor of MLCK, in vivo the inhibitor retards the growth of prostate tumours, acts as an adjuvant to etoposide stimulating its ability to prevent growth of established tomours, overview
ML-7
-
-
ML-7
-
inhibition of MLCK induces apoptosis in adherent cells and disrupts cell-cell adhesion
ML-7
-
specificity, overview, ML-7 changes action potential amplitude and voltage-gated Ca2+ channel current nonspecifically, but does not affect calcimycin-induced release and vesicle readily releasable pool RRP recovery after depletion
ML-7
-
a specific inhibitor, in vivo the inhibitor enhances the short-term and long-term memory, when applied before fear conditioning, but not immediately afterwards, the inhibitor has no effet on memory retrieval and behaviour when the training stimuli are presented in a non-associative manner
ML-7
specific inhibitor for MLCK
ML-7
-
ie. 1-(5-iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazapine hydrochloride, a specific inhibitor of MLCK, ML-7 is able to depress the cell proliferation and migration in vivo using two parallel breast cancer cell lines. ML-7 not only blocks MLCK but also downregulates activated ERK1/2 with downstream effectors of beta-catenin-survivin-cyclin D1-c-Myc, overview
ML-7
-
a specific MLCK inhibitor
ML-7
-
i.e. 1-(5-iodonapthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine
ML-7
-
specific MLCK inhibitor
ML-7
-
selective MLCK inhibitor
ML-9
-
inhibition of MLCK induces apoptosis in adherent cells and disrupts cell-cell adhesion
ML-9
impairs the plasma membrane localization of TRPC5 channels; impairs the plasma membrane localization of TRPC5 channels
ML-9
-
i.e. 1-(5-chloronapthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine, inhibits MLCK and paracellular permeability increases in several intestinal epithelial models
ML-9
-
i.e. 1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine, inhibits MLCK and paracellular permeability increases in several intestinal epithelial models
ML-9
-
-
morin
-
IC50: 0.028 mM
MS-347a
-
from Aspergillus sp. KY52178, structurally related to sydowinin B, irreversible, inhibition of calmodulin-dependent and independent activity, IC50: 0.0092 mM
myricetin
-
IC50: 0.006 mM
myristoylcarnitine chloride
-
-
myristoylcholine iodide
-
IC50: 0.02 mM
N-Alkyl-N,N-dimethyl-3-ammonio-1-propanesulfonates
-
zwittergents 3-14 and 16
N-methyloctadecylamine
-
IC50: 0.01 mM
Naphthalene sulfonamide derivatives
-
-
-
Octadecylamine
-
IC50: 0.011 mM
Okanin
-
-
oleic acid
-
-
oleylamine
-
IC50: 0.006 mM
p21-activated kinase 1
-
-
-
palmitoylcarnitine chloride
-
-
-
palmitoylcholine iodide
-
IC50: 0.014 mM
-
Peptide 18
-
i.e. H-RKKYKYRRK-NH2, specifically inhibits the enzyme and prevents sperm chromatin-induced cortical reorganization in eggs in vivo
-
phosphate
-
up to 0.1 M, weak
protein kinase inhibitor PKI
-
-
-
pseudobabtisin
-
-
-
purpurin
-
IC50: 0.025 mM
quercetagetin
-
IC50: 0.026 mM
quercetin
-
IC50: 0.006 mM
quercetrin
-
IC50: 0.137 mM
quinalizarin
-
IC50: 0.053 mM
quinizarin
-
IC50: 0.026 mM
Rho GDI
-
-
-
RKKYKYRRK-NH2
-
i.e. D PIK
RKKYKYRRK-NH2
-
-
RRKWQKTGHAVRAIGRL
-
-
-
RRKYQKTGHAVRAIGRL
-
-
-
rutin
-
IC50: 0.32 mM
SKDRMKKYMARRKWQKTGHAVRAI
-
autoregulatory pseudosubstrate sequence of smooth muscle enzyme, residues 787-810
sodium alkylsulfate
-
-
sodium dodecylsulfate
-
IC50: 0.049 mM
Sodium octadecylsulfate
-
IC50: 0.043 mM
Sodium tetradecylsulfate
-
IC50: 0.038 mM
SQRLLKKYLMKRRWKKNFIAVSAA
-
autoregulatory pseudosubstrate sequence of the skeletal muscle enzyme, residues 570-593
stearoylcarnitine chloride
-
-
stearoylcholine iodide
-
IC50: 0.013 mM
tetradecylamine
-
IC50: 0.012 mM
Tetradecyltrimethylammonium bromide
-
; IC50: 0.011 mM
tricetin
-
IC50: 0.012 mM
tridecylamine
-
IC50: 0.019 mM
unsaturated fatty acids
-
irreversible by Ca2+/calmodulin
-
W-7
-
calmodulin/enzyme-specific inhibitor, attenuates cell migration in vivo
W-7
-
40-60% inhibition in vivo
Wortmannin
-
i.e. MS-54, IC50: 0.0019 mM, irreversible, highly selective, kinetics, high concentrations of ATP protect
Wortmannin
-
complete inhibition at 0.01 mM
Y-27632
-
40-60% inhibition in vivo
MLCK pepitde
-
complete inhibition
additional information
-
the enzyme is inhibited by its regulatory subunit masking the active site, autoregulation by a pseudosubstrate mechanism, protein substrate-pseudosubstrate interactions, overview
-
additional information
-
synthesis of peptides behaving as pseudosubstrates, determination of inhibitory potential
-
additional information
autoregulatory domain extends from Asn780 to Arg808. The peptide Leu774 to Ser787 does not inhibit smMLCK, peptides of similar or shorter length from the pseudosubstrate region, Ser787 to Val807, are potent inhibitors
-
additional information
-
the enzyme is regulated by an autoinhibitory domain
-
additional information
-
no inhibition by 3',5'-cAMP
-
additional information
-
no inhibition by 3',5'-cAMP; no inhibition by AMP
-
additional information
-
structural requirements of autoinhibition of myosin light chain kinase
-
additional information
-
no inhibition by epicatechin, pseudobabtisin, 4-dimethylaminobenzaldehyde
-
additional information
-
no inhibition by diacylglycerol, phosphatidylserine
-
additional information
-
inhibition by autophosphorylation
-
additional information
-
residues 283-288 function as an autoinhibitory domain, autoinhibition is fully relieved by Thr166 phosphorylation
-
additional information
-
no inhibition in vivo by Y-27632
-
additional information
-
no inhibition by nocodazone
-
additional information
-
non-kinase, inhibitory activity of N-terminal, actin-binding domain of MLCK
-
additional information
-
oxytoxin and phorbol ester do not affect the enzyme activity. Non-kinase, inhibitory activity of N-terminal, actin-binding domain of MLCK
-
additional information
-
the inhibitor of ERK1/2, PD98059, downregulates the levels of MLCK and phosphorylated myosin light chain
-
additional information
-
PAK phosphorylation of MLCK by p21-activated kinase, a kinase activated by the Rho family of GTPases, can inhibit MLCK activity
-
additional information
-
protein kinase A inhibits MLCK and myosin loght chain dephosphorylation, the latter via effects on the Rho kinase/MLC phosphatase pathway, reversible by KT5720, an inhibitor of PKA. Inhibition of MLC phosphorylation leads to beta2-integrin activation and adhesion, overview
-
additional information
-
inhibitor effects on enzyme activity in intestinal tissues, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Autophosphorylation
activation
-
Autophosphorylation
-
no activation
-
Autophosphorylation
-
no activation
-
Calmodulin
-
a calmodulin-sensitive MLCK isozyme
Calmodulin
activates, dependent on; activates, dependent on
Calmodulin
-
-
Calmodulin
-
the enzyme is dependent on Ca2+ and calmodulin
Calmodulin
-
Calmodulin
-
dependent on, antagonizes the binding to actin, residues 26-41 of MLCK act as a CaM-binding region that regulates the binding of MLCK to actin, Ca/CaM is also binds at the Ala796-Ser815 sequence to regulate the kinase activity MLCK
Calmodulin
-
dependent on, antagonizes the binding to actin
Calmodulin
-
dependent on
Calmodulin
-
required
Calmodulin
-
required
Calmodulin
-
dependent on
Calmodulin
-
dependent on
eotaxin
-
activates the enzyme 4fold, induces enzyme phosphorylation
-
F-actin
-
binding via the DFRXXL motifs in the N-terminal region of isozymes S-MLCK and L-MLCK, the latter also uses the six Ig-like modules in its N-terminal extension for binding, the first two of which are minimally required for microfilament binding, overview
-
interleukin 1
-
i.e IL-1, activates enzyme activity in endothelial cells
-
phenylephrine
-
80% activation at up to 0.1 mM
Phosphorylation
-
activation, smooth muscle enzyme, not bovine cardiac enzyme
-
Phosphorylation
-
no activation
-
thrombin
-
stimulates endothelial isozyme activity in endothelium, and increases EC MLCK interaction with macrophage migration inhibition factor MIF
-
TNFalpha
-
stimulates endothelial isozyme activity in endothelium, and increases EC MLCK interaction with macrophage migration inhibition factor MIF
-
tumor necrosis factor-alpha
-
i.e TNF-alpha, activates enzyme activity in endothelial cells
-
tumor necrosis factor-alpha
-
-
-
isoprenaline
-
80% activation at up to 0.01 mM
additional information
-
autoregulation by a pseudosubstrate mechanism, overview
-
additional information
no activation by cAMP; no activation by cGMP
-
additional information
-
no activation by cAMP
-
additional information
-
no activation by Ca2+ plus beta-lactoglobulin, cytochrome c, troponin C or parvalbumin; no activation by cAMP
-
additional information
-
no activation by mild proteolysis
-
additional information
-
no activation by cAMP; no activation by phosphatidylserine
-
additional information
-
the enzyme is activated by phosphorylation through mitogen activated protein kinase MAPK
-
additional information
-
140fold activation through phosphorylation at Thr166
-
additional information
-
the endothelial isozyme EC MLCK contains a unique N-terminal sequence which is a target for upregulation by signaling cascade components such as p60src
-
additional information
-
myocardin, a cardiac SAP, SAF-A/B, acinus, PIAS, domain-containing protein, is important in regulation of enzyme expression, overview, induction of enzyme expression by the thyrotroph embryonic factor binding to the the CArG box of the promoter, TEF
-
additional information
-
tumor necrosis factor-alpha induces enzyme activity in vivo
-
additional information
-
electric stimulation of intact skeletal muscles activate the enzyme activity, overview
-
additional information
-
oxytoxin and phorbol ester do not affect the enzyme activity
-
additional information
-
activation of the RhoA pathway can inhibit myosin phosphatase activity, thereby enhancing the effects of the kinase-mediated phosphorylation of myosin light chain
-
additional information
-
oxidized low density lipoprotein induces the enzyme expression and activation in umbilical vein endothelial cells, which is inhibted by melatonin and PD98059
-
additional information
-
epilactose induces myosin light chain phosphorylation via MLCK
-
additional information
-
treatment with pentoxifylline attenuates activation of MLCK likely through its ability to decrease local tumor necrosis factor-alpha synthesis and nucleat factor kappaB activation after burn
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.05
ATP
-
24C, pH 7.3
0.073
ATP
-
30C, pH 7.5
0.075
ATP
-
-
0.121
ATP
-
23-25C, pH 7.5
0.167
ATP
-
-
0.175
ATP
-
30C, pH 8
0.22
ATP
-
30C, pH 7
0.05 - 0.063
bovine cardiac muscle myosin light chain
-
-
0.0075
BpaKKRAARATSNVFA
-
25C, pH 7, Bpa is the photoreactive amino acid p-benzoylphenylalanine
-
0.000002
Calmodulin
-
30C, pH 6.8
0.000002
Calmodulin
-
30C, pH 7.5
0.004
Dictyostelium myosin
22C, pH 7.5
-
0.05 - 0.063
isolated myosin light chain
-
25C, pH 7.6
0.11
kemptamide
-
30C, pH 6.8
0.0084
KKRAARATSNVFA
-
25C, pH 7
0.004
myosin
myosin from Dictyostelium
-
0.0043
myosin light chain
cardiac MLCK
0.005 - 0.0095
myosin light chain
-
-
0.005 - 0.0095
myosin light chain
-
30C, pH 7.2
0.005 - 0.0095
myosin light chain
-
-
0.005 - 0.0095
myosin light chain
-
-
0.005
myosin light chain
-
24C, pH 7.3
0.005
myosin light chain
-
-
0.011 - 0.02
myosin light chain
-
30C, pH 7
0.018
myosin light chain
-
23-25C, pH 7.5
0.018
myosin light chain
-
30C, pH 7.5
0.019
myosin light chain
-
25C, pH 7.6, bound to myosin
0.0067
myosin regulatory light chain
-
pH 7, M968P
-
0.011
myosin regulatory light chain
-
pH 7, A986P
-
0.014
myosin regulatory light chain
-
pH 7, wild type
-
0.1 - 0.2
rabbit white skeletal muscle myosin P-light chain
-
27C, pH 7.6
-
0.094 - 0.096
skeletal muscle myosin light chain
-
-
0.02 - 0.027
turkey gizzard myosin light chain
-
30C, pH 7.6, ATP
0.02 - 0.027
turkey gizzard myosin light chain
-
30C, pH 6.8
0.02 - 0.027
turkey gizzard myosin light chain
-
30C, pH 7
0.04
turkey gizzard myosin light chain
-
pH 7.5, myometrium enzyme
0.0156
Limulus myosin light chain
-
25C, pH 7.5
additional information
additional information
-
in the presence of wortmannin
-
additional information
additional information
-
kinetic constants for enzymes from various sources with different myosin light chains as substrates
-
additional information
additional information
-
Michaelis-Menten kinetics, kinetic scheme of the mathematical model considers interactions among Ca2+, calmodulin and MLCK, and the well-known 4-state actomyosin latch bridge model, whereby a link between them is accomplished by the conservation relation of all species of MLCK, detailed overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5.17
myosin light chain
Homo sapiens
-
23-25C, pH 7.5
16
myosin light chain
Oryctolagus cuniculus
-
25C, pH 7.6, isolated
19
myosin light chain
Oryctolagus cuniculus
-
25C, pH 7.6, bound to myosin
88
myosin light chain
Oryctolagus cuniculus
-
25C, pH 7.6, isolated, freshly prepared
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
Ki values of the pseudosubstrates in nano- to micromolar range
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.44
(+)-catechin
Gallus gallus
-
IC50: 0.44 mM
0.32
(-)-epicatechin
Gallus gallus
-
IC50: 0.32 mM
0.063
1,12-diaminododecane
Gallus gallus
-
IC50: 0.063 mM
0.018
1,4-diaminoanthraquinone
Gallus gallus
-
IC50: 0.018 mM
0.049
1-hexadecylpyridinium bromide
Gallus gallus
-
IC50: 0.049 mM
0.262
3',4'-dihydroxyflavone
Gallus gallus
-
IC50: 0.262 mM
0.001
3,3',4'-trihydroxyflavone
Gallus gallus
-
IC50: 0.001 mM
0.024
5,4'-dihydroxyflavone
Gallus gallus
-
IC50: 0.024 mM
0.043
5,7-dihydroxyflavone
Gallus gallus
-
IC50: 0.043 mM
0.02
7,8,3',4'-tetrahydroxyflavone
Gallus gallus
-
IC50: 0.02 mM
0.014
alizarin
Gallus gallus
-
IC50: 0.014 mM
0.037
anthraflavic acid
Gallus gallus
-
IC50: 0.037 mM
0.023
apigenin
Gallus gallus
-
IC50: 0.023 mM
0.02
chrysazine
Gallus gallus
-
IC50: 0.02 mM
0.006
D-Sphingosine
Gallus gallus
-
IC50: 0.006 mM
0.2
decylamine
Gallus gallus
-
IC50: 0.2 mM
0.17
dihydroapigenin
Gallus gallus
-
IC50: 0.17 mM
0.18
dihydrofisetin
Gallus gallus
-
IC50: 0.18 mM
0.08
dihydroquercetin
Gallus gallus
-
IC50: 0.08 mM
0.008
dihydrosphingosine
Gallus gallus
-
erythro- and threo-dihydrosphingosine, IC50: 0.008 mM
0.008
Dimethyldioctadecylammonium bromide
Gallus gallus
-
IC50: 0.008 mM
0.055
dioctylamine
Gallus gallus
-
IC50: 0.055 mM
0.083
dodecylamine
Gallus gallus
-
IC50: 0.083 mM
0.078
dodecyltrimethylammonium bromide
Gallus gallus
-
IC50: 0.078 mM
0.008
emodin
Gallus gallus
-
IC50: 0.008 mM
0.005
fisetin
Gallus gallus
-
IC50: 0.005 mM
0.02
galangin
Gallus gallus
-
IC50: 0.02 mM
0.016
hexadecylamine
Gallus gallus
-
IC50: 0.016 mM
0.011
Hexadecyltrimethylammonium bromide
Gallus gallus
-
IC50: 0.011 mM
0.008
kaempferid
Gallus gallus
-
IC50: 0.008 mM
0.00045
kaempferol
Bos taurus
-
i.e. 3,5,7-trihydroxy-2-(4-hydroxyphenyl)-1-benzopyran-4-one, IC50: 0.00045 mM
0.004
kaempferol
Gallus gallus
-
IC50: 0.004 mM
0.12
lauroylcholine iodide
Gallus gallus
-
IC50: 0.12 mM
0.026
luteolin
Gallus gallus
-
IC50: 0.026 mM
0.04
merocyanine dye (C16)
Gallus gallus
-
IC50: 0.040 mM
0.002
mitoxanthrone
Gallus gallus
-
IC50: 0.002 mM
0.028
morin
Gallus gallus
-
IC50: 0.028 mM
0.0092
MS-347a
Gallus gallus
-
from Aspergillus sp. KY52178, structurally related to sydowinin B, irreversible, inhibition of calmodulin-dependent and independent activity, IC50: 0.0092 mM
0.006
myricetin
Gallus gallus
-
IC50: 0.006 mM
0.02
myristoylcholine iodide
Gallus gallus
-
IC50: 0.02 mM
0.01
N-methyloctadecylamine
Gallus gallus
-
IC50: 0.01 mM
0.011
Octadecylamine
Gallus gallus
-
IC50: 0.011 mM
0.006
oleylamine
Gallus gallus
-
IC50: 0.006 mM
0.014
palmitoylcholine iodide
Gallus gallus
-
IC50: 0.014 mM
-
0.025
purpurin
Gallus gallus
-
IC50: 0.025 mM
0.026
quercetagetin
Gallus gallus
-
IC50: 0.026 mM
0.006
quercetin
Gallus gallus
-
IC50: 0.006 mM
0.137
quercetrin
Gallus gallus
-
IC50: 0.137 mM
0.053
quinalizarin
Gallus gallus
-
IC50: 0.053 mM
0.026
quinizarin
Gallus gallus
-
IC50: 0.026 mM
0.029
RKKYKYRRK-NH2
Homo sapiens
-
-
0.32
rutin
Gallus gallus
-
IC50: 0.32 mM
0.049
sodium dodecylsulfate
Gallus gallus
-
IC50: 0.049 mM
0.043
Sodium octadecylsulfate
Gallus gallus
-
IC50: 0.043 mM
0.038
Sodium tetradecylsulfate
Gallus gallus
-
IC50: 0.038 mM
0.013
stearoylcholine iodide
Gallus gallus
-
IC50: 0.013 mM
0.012
tetradecylamine
Gallus gallus
-
IC50: 0.012 mM
0.011
Tetradecyltrimethylammonium bromide
Gallus gallus
-
IC50: 0.011 mM
0.012
tricetin
Gallus gallus
-
IC50: 0.012 mM
0.019
tridecylamine
Gallus gallus
-
IC50: 0.019 mM
0.0019
Wortmannin
Gallus gallus
-
i.e. MS-54, IC50: 0.0019 mM, irreversible, highly selective, kinetics, high concentrations of ATP protect
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1.8
-
myosin light chain, isolated
1.8
-
-
2.51
-
myosin light chain, in native myosin
5.4
-
rabbit uterine myosine
6.1 - 8.7
-
-
7.7
-
turkey gizzard myosine
8.1 - 8.9
-
-
13
-
BpaKKRAARATSNVFA as substrate
18.5
-
-
19.9
-
KKRAARATSNVFA as substrate
24
-
skeletal muscle
additional information
-
1280 pmol/min/pmol
additional information
-
quantification of enzyme activity using a Ca2+/calmodulin-dependent fluorescent biosensor MLCK in vitro or in vivo, overview
additional information
-
-
additional information
-
-
additional information
-
-
additional information
quantitative determination of phosphorylated myosin light chain
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7
-
assay at
7.4
-
assay at
7.5
-
assay at
7.5
-
assay at
7.5
assay at
7.8 - 8
-
-
8.1
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.7 - 8.2
-
about half-maximal activity at pH 5.7 and 8.2, with a small shoulder of 77% of maximal activity at 7-7.5
6.3 - 9.2
-
about half-maximal activity at pH 6.3 and 9.2
6.8 - 8.8
-
about half-maximal activity at pH 6.8 and about 75% of maximal activity at pH 8.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
23 - 25
-
assay at
24
-
assay at
28
-
assay at
30
-
assay at
30
-
assay at
30
-
assay at
30
-
assay at
30
-
assay at
37
-
assay at
37
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5
-
isoelectric focusing, pH 3-10, 4% polyacrylamide gel or flat bed isoelectric focusing with Sephadex G-200
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
thoracic
Manually annotated by BRENDA team
maximal stimulation-induced in situ myosin light chain kinase activity is upregulated in fetal compared to adult ovine carotid arteries
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, including brain
Manually annotated by BRENDA team
-
a SV40-infected endothelial cell line
Manually annotated by BRENDA team
-
endobronchial tissue
Manually annotated by BRENDA team
endothelial cell, 2 isozymes MLCK1 and MLCK2
Manually annotated by BRENDA team
-
cMLCK is exclusively expressed in cardic muscle, smMLCK expresses in almost every tissue, including cardiac muscles
Manually annotated by BRENDA team
-
from embryos, in myofibril precursors and sarcomeric Z-lines, co-localization with myosin IIB
Manually annotated by BRENDA team
fetal and adult, quantification of the enzyme and its product
Manually annotated by BRENDA team
-
a cerebral microvascular endothelial cell line
Manually annotated by BRENDA team
-
epithelium
Manually annotated by BRENDA team
-
from patients who had not been treated with chemotherapy or radiotherapy, freshlys collected at the time of surgical resection for malignant or non-inflammatory disease
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, including colon
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
-
-
Manually annotated by BRENDA team
-
polarized, arrested in MII
Manually annotated by BRENDA team
-
at the equator of dividing cells
Manually annotated by BRENDA team
-
endothelial isozyme EC MLCK
Manually annotated by BRENDA team
well differentiated cells, isozyme MLCK1
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
-
-
Manually annotated by BRENDA team
-
pregnant sheep myometrium, turkey and chicken gizzard enzyme are immunologically related
Manually annotated by BRENDA team
-
rat pancreatic and turkey gizzard enzyme are immunologically related
Manually annotated by BRENDA team
-
left ventricle tissue, MLCK occurs in a cross-striated pattern overlapping with the distribution of alpha-actin
Manually annotated by BRENDA team
-
specific cardiac/ventricular isozyme cardiac-MLCK
Manually annotated by BRENDA team
-
specific cardiac/ventricular isozyme cardiac-MLCK encoded by gene MLCK3, expression analysis in healthy hearts and hearts from patients with heart failure
Manually annotated by BRENDA team
cardiac-specific myosin light chain kinase
Manually annotated by BRENDA team
-
cultured hippocampal neurons
Manually annotated by BRENDA team
-
a fibrosarcoma cell line
Manually annotated by BRENDA team
-
ileal epithelial MLCK expression is mildly upregulated in inactive inflammatory bowel disease, and highly upregulated in active IBD
Manually annotated by BRENDA team
-
epithelium, expression of MLVK is upregulated by inflammatory bowel disease
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
mucosa
-
Manually annotated by BRENDA team
-
expression analysis of MLCK in healthy and streptozotocin-induced diabetic rats, expression in glomerulus, renal tubule and glomerular arteriolar, glomerulus expression is increased in diabetic rats, overview
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, including kidney
Manually annotated by BRENDA team
-
myofibroblast-like stellate cell line, previously called Ito cells, lipocytes, or fat-storing cells
Manually annotated by BRENDA team
-
polymorphonuclear and alveolar
Manually annotated by BRENDA team
-
distribution analysis
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, including liver
Manually annotated by BRENDA team
-
LNCaP cells express both long (non-muscle) and short (smooth muscle) isoforms of MLCK
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, including lung
Manually annotated by BRENDA team
-
a prostate cancer cell line
Manually annotated by BRENDA team
-
smooth muscle
Manually annotated by BRENDA team
-
telokin, the carboxyl terminus of the smooth muscle myosin light chain kinase which is expressed as an independent protein, is expressed in some smooth muscle tissues but not in aortic smooth muscle or in any non-muscle tissues
Manually annotated by BRENDA team
-
striated, a distinct MLCK isozyme
Manually annotated by BRENDA team
-
expression of obscurin-MLCK-like kinase is restricted to muscles in the myonucleus
Manually annotated by BRENDA team
-
adult, MLCK occurs in a cross-striated pattern overlapping with the distribution of alpha-actin
Manually annotated by BRENDA team
-
expression analysis, overview
Manually annotated by BRENDA team
-
pregnant sheep myometrium, turkey and chicken gizzard enzyme are immunologically related
Manually annotated by BRENDA team
-
cultured hippocampal neurons
Manually annotated by BRENDA team
-
rat pancreatic and turkey gizzard enzyme are immunologically related
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, including plaeta
Manually annotated by BRENDA team
-
pectoralis muscle
Manually annotated by BRENDA team
-
skeletal muscle expresses a specific MLCK called skeletal-muscle-type myosin light chain kinase, smooth muscle myosin light chain kinase expresses in almost every tissue including skeletal muscles
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, incnluding small intestine
Manually annotated by BRENDA team
-
uterus, trachea, aorta, ileum, gizzard
Manually annotated by BRENDA team
-
long isozyme L-MLCK and short isozyme S-MLCK
Manually annotated by BRENDA team
-
specific smooth muscle isozyme smMLCK encoded by gene MLCK2
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, including smooth muscles
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, including spleen
Manually annotated by BRENDA team
-
smMLCK expresses in almost every tissue, including thymus
Manually annotated by BRENDA team
-
the long isoform of MLCK, MLCK-210, is the predominant isoform expressed in vascular endothelial cells
Manually annotated by BRENDA team
-
a mammary adenocarcinoma cell line
Manually annotated by BRENDA team
additional information
-
myosin light chain kinases in smooth muscle and non-muscle tissues are the same protein
Manually annotated by BRENDA team
additional information
-
the human MLCK gene yields multiple nonmuscle MLCK isoforms by alternative splicing of its transcribed mRNA precursor with differential distribution of these isoforms in various human tissues and cells
Manually annotated by BRENDA team
additional information
expression profile of isozyme MLCK1; expression profile of isozymes MLCK1 and MLCK2
Manually annotated by BRENDA team
additional information
-
two isozymes: the endothelial, long or nonmuscle 220-kDa MLCK and a short, smooth muscle 130-kDa MLCK, mechanism of regulation of tissue-specific mylk gene expression, overview
Manually annotated by BRENDA team
additional information
-
tissue distribution analysis, overview
Manually annotated by BRENDA team
additional information
-
tissue dependent MLCK expression analysis of 26 inflammatory bowel disease patients
Manually annotated by BRENDA team
additional information
-
subcellular localization
Manually annotated by BRENDA team
additional information
-
developmental pattern of obscurin and obscurin-associated kinase expression, overview
Manually annotated by BRENDA team
additional information
-
nonmuscle isozyme
Manually annotated by BRENDA team
additional information
-
MLCK's are not expressed in leukocytes
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
F-actin-associated along cellular stress fibres
Manually annotated by BRENDA team
subcellular localization study
Manually annotated by BRENDA team
-
of lateral amygdala
Manually annotated by BRENDA team
additional information
isozyme MLCK1 is predominantly located in the perijunctional actomyosin ring in villus enterocytes
-
Manually annotated by BRENDA team
additional information
-
peripherally in hepatocytes, rarely in cytoplasm
-
Manually annotated by BRENDA team
additional information
-
subcellular localization study of MLCK in embryonic cardiomyocytes, overview
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
34000
; gel filtration
491266
37000
-
PAGE, 2 forms of myosin light chain kinase
640637
39000
-
PAGE, 2 forms of myosin light chain kinase
640637
77000
-
gel filtration
640614
85000
-
gel filtration
640621
103000
-
sedimentation equilibrium method
640615
108000
-
smooth muscle isozyme
663442
108000 - 125000
-
short myosin light chain kinase
661665
124000
-
sedimentation equilibrium centrifugation
640630, 640631, 640645
125700
-
calculated from sequence of DNA
491268
127000
-
sucrose density gradient centrifugation
640623
130000
-
gel filtration
640630
130000 - 150000
-
smooth muscle isozyme
663442
140000
-
endogenous MLCK, SDS-PAGE
709692
150000
-
gel filtration and sedimentation studies
640619
150000
-
-
661920
155000
-
491294
155000
-
-
684910
190000
-
exogenous (biosensor) MLCK, SDS-PAGE
709692
210000
-
-
661664
210000
-
long myosin light chain kinase
661665
210000
-
endothelial isozyme
663442
211000
-
deduced from nucleotide sequence
491283
214000
-
endothelial isozyme
663442
215000
-
-
688628
926000
-
deduced from nucleotide sequence, stretchin-MLCK
640656
additional information
-
amino acid sequence of an active fragment of rabbit skeletal muscle myosin light chain kinase
491222
additional information
-
amino acid sequence of rabbit skeletal muscle myosin light chain kinase
491223
additional information
-
amino acid composition of rabbit
640615
additional information
-
-
640620
additional information
-
-
640630
additional information
-
different molecular weights may be due to high sensitivity to proteolysis during purification; relative masses of various animal skeletal muscle enzymes
640645
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 155000, SDS-PAGE
?
-
x * 152000, SDS-PAGE, recombinant enzyme
?
-
x * 136000, SDS-PAGE, enzyme from smooth muscle
?
-
x * 92000, SDS-PAGE
?
-
x * 105000, SDS-PAGE
?
-
x * 94000, SDS-PAGE
?
-
x * 152000, SDS-PAGE
?
-
x * 160000, SDS-PAGE
?
-
x * 135000, SDS-PAGE
?
-
SDS-PAGE
?
-
x * 130000, SDS-PAGE
?
-
x * 138000, SDS-PAGE
?
-
x * 214000, SDS-PAGE, from endothelium
?
-
x * 190000, recombinant Ca2+/calmodulin-dependent fluorescent biosensor MLCK, SDS-PAGE
?
-
x * 190000, recombinant enzyme mutant MLCK1745, SDS-PAGE, x * 210000, wild-type enzyme and mutant MLCKATPdel, SDS-PAGE
monomer
1 * 34000, SDS-PAGE
monomer
-
1 * 214000, SDS-PAGE, endothelial enzyme
monomer
-
1 * 77000, SDS-PAGE
monomer
-
1 * 94000, SDS-PAGE
monomer
-
1 * 85000, SDS-PAGE
monomer
-
1 * 37000, SDS-PAGE 2 forms of myosin light chain kinase; 1 * 39000, SDS-PAGE, 2 forms of myosin light chain kinase
monomer
-
1 * 130000, SDS-PAGE
monomer
Dictyostelium discoideum Ax-3
-
1 * 34000, SDS-PAGE
-
additional information
-
the carboxyl terminus of the smooth muscle myosin light chain kinase is expressed as an independent protein, telokin
additional information
-
gel electrophoresis in various buffers gives different molecular weights
additional information
-
skeletal muscle myosin light chain kinases from different species share more identity than skeletal muscle and smooth muscle myosin light chain kinases from the same species
additional information
-
skeletal muscle enzyme structure: overall asymmetric shape, globular head and tail region
additional information
-
different molecular weights may be due to high sensitivity to proteolysis during purification
additional information
-
multienzyme complex with smooth muscle myosin light chain phosphatase
additional information
-
the high moecular weight endothelial enzyme is stably associated to a complex containing p60src and 80000 cortactin
additional information
-
MLCK-210 contains an additional actin-binding domain at the N-terminus
additional information
-
the endothelial isozyme EC MLCK contains a unique N-terminal sequence which is a target for upregulation by signaling cascade components such as p60src, EC MLCK splice variants doamin structure
additional information
-
the endothelial isozyme possesses a catalytic domain including the actin-binding domain and the calmodulin binding domain, like the smooth muscle isozyme, but also a protein-protein interaction domain including SH2 and SH3 domains
additional information
-
MLCK domain structure, a fusion protein consisting of the N-terminal actin-binding domain, the central catalytic domain, and the C-terminal myosin-binding domain
additional information
-
MLCK domain structure, a fusion protein consisting of the Nterminal actin-binding domain, the central catalytic domain, and the C-terminal myosin-binding domain
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
-
protein kinase A phosphorylates MLCK in its regulatory domain at Ser1021 and Ser1034. The kinase activity of MLCK phosphorylated at Ser1021 is reduced, whereas that of MLCK phosphorylated at Ser1034 is not reduced
phosphoprotein
-
the enzyme is 140fold activated through phosphorylation at Thr166 by a recombinant constitutively active Ca2+/calmodulin-dependent protein kinase kinase, the enzyme performs autophosphorylation at Thr289, subsequent to Thr166 phosphorylation by the protein kinase kinase, and at several serine residues, leading to release of the autoinhibitory domain from the catalytic core, identification of phosphorylation sites
phosphoprotein
-
protein kinase A phosphorylates MLCK in its regulatory domain at Ser815 and Ser828. The kinase activity of MLCK phosphorylated at Ser815 is reduced, whereas that of MLCK phosphorylated at Ser828 is not reduced
phosphoprotein
-
diffential phosphorylation by Aurora B on serine residues, e.g. in the enzyme's IgG domain, during interphase and mitosis has regulatory function, phosphorylation sites for Aurora B on the enzyme, overview
phosphoprotein
-
eotaxin induces enzyme phosphorylation by serine/threonine protein kinases e.g. by MAP kinases ERK1/2 and p38 at Thr43, phosphorylation is required for activation of the enzyme
phosphoprotein
-
PAK phosphorylation of MLCK by p21- activated kinase, a kinase activated by the Rho family of GTPases, can inhibit MLCK activity
phosphoprotein
-
the enzyme is phosphorylated and activated by mitogen activated protein kinase MAPK
phosphoprotein
the cardiac-specific isozyme probably performs also autophosphorylation
phosphoprotein
-
PAK phosphorylation of MLCK by p21- activated kinase, a kinase activated by the Rho family of GTPases, can inhibit MLCK activity
phosphoprotein
-
diffential phosphorylation by Aurora B on serine residues during interphase and mitosis has regulatory function, phosphorylation sites for Aurora B on the enzyme, overview
phosphoprotein
-
PAK phosphorylation of MLCK by p21- activated kinase, a kinase activated by the Rho family of GTPases, can inhibit MLCK activity
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
twitchin kinase, bilobal structure analysis
-
isolution structure of a calmodulin-target peptide complex by multidimensional NMR
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5
-
rapid inactivation below
640614, 640615
5
-
30-60 min, about 50% loss of activity
640621
6.3 - 8
-
stable in 10% sucrose
640614
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol and Tween 40 stabilize
-
protease inhibitors with broad specificity and glycerol stabilize during initial purification, unstable to further purification
-
repeated freeze-thawing decreases activity
-
unstable upon lyophilization
-
protease inhibitors stabilize during purification
-
repeated freeze-thawing decreases activity
-
EGTA prevents Ca2+-dependent proteolysis during initial purification
-
MgCl2 is critical for kinase extraction from myofibrils
-
protease inhibitors stabilize during purification
-
repeated freeze-thawing decreases activity
-
repeated freeze-thawing decreases activity
-
unstable upon lyophilization
-
protease inhibitors stabilize during purification
-
protease inhibitors stabilize during purification
-
repeated freeze-thawing decreases activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30C, 30% loss of activity within 3 weeks
-
-70C, at least 6 months
-
-20C, in 5% w/v sucrose, several weeks
-
proteolysis occurs even on storage at -80C, myometrium enzyme
-
-70C, 1% bovine serum albumin, more than 2 months
-
-20C, quite unstable on storage
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
calmodulin-Sepharose column chromatography, DEAE 650M Toyopearl column chromatography, and Superose 6 gel filtration
purified to homogeneity from a number of vertebrate muscles and partially purified from non-muscle tissues
-
recombinant GST-tagged C-terminal enzyme fragment by glutathione affinity chromatography
-
purified to homogeneity from a number of vertebrate muscles and partially purified from non-muscle tissues
-
butyl-Toyopearl column chromatography and Sephacryl S-200 gel filtration
-
native enzyme from gizzard muscle
-
purified to homogeneity from a number of vertebrate muscles and partially purified from non-muscle tissues
-
partial, affinity chromatography on calmodulin-Sepharose
-
partially from eosinophils
-
purified to homogeneity from a number of vertebrate muscles and partially purified from non-muscle tissues
-
recombinant GFP-fusion enzyme from HeLa cells
-
recombinant GST-fusion isozyme EC MLCK by affinity chromatgraphy
-
recombinant wild-type and mutant enzymes from transfected bovine endothelial cells
-
affinity chromatography on calmodulin-Sepharose
-
purified to homogeneity from a number of vertebrate muscles and partially purified from non-muscle tissues
-
affinity chromatography on calmodulin-Sepharose
-
recombinant His-tagged wild-type and mutant isozymes from Escherichia coli strain Bl21(DE3) by nickel affinity chromatograpyh
-
purified to homogeneity from a number of vertebrate muscles and partially purified from non-muscle tissues
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression of a C-terminal enzyme fragment comprising residues 860-1176 as GST-fusion protein in Escherichia coli strain Bl21(DE3), the GST-tag slightly reduces the activity of the recombinant enzyme fragment compared to the untagged fragment
-
expression of wild-type full-length MLCK and of several enzyme fragments in Escherichia coli with the use of a cold-shock promoter
-
isolation of cDNA
genes MYLK2 and MYLK3, DNA and amino acid sequence determination and analysis, expression analysis in heart
-
the full-length enzyme as well as a truncated form lacking the putative auto-inhibitory domain are expressed in bacterial cells
-
DNA and amino acid sequence determination and analysis
-
domain organization of chicken gizzard myosin light chain kinase deduced from a cloned cDNA
-
expressed in Escherichia coli BL21(DE3) cells
-
expression in BL21(DE3) cells as GFP fusion protein; transient expression as GFP fusion protein in A7r5, HeLa, NIH3T3 or COS-7 cells
-
expression of His6-tagged MLCK-210 nad His-tagged N452-MLCK in Escherichia coli, expression of GFP-tagged MLCK-210 and GFP-tagged N452-MLCK in CV-1 African green monkey kidney cells
-
skeletal muscle enzyme
-
DNA and amino acid sequence determination and analysis, expressionin HEK-293 cells, co-expression with TRPC5 from Drosophila melanogaster; DNA and amino acid sequence determination and analysis, expressionin HEK-293 cells, co-expression with TRPC5 from Drosophila melanogaster
DNA sequence determination and analysis of splicing variant 1 of long endothelial enzyme, large scale expression of endothelial isozyme MLCK1 in Spodoptera frugiperda Sf9 cells via Baculovirus transfection system; DNA sequence determination and analysis of splicing variant 2 of long endothelial enzyme, large scale expression of endothelial isozyme MLCK2 in Spodoptera frugiperda Sf9 cells via Baculovirus transfection system
DNA sequence determination and analysis, overexpression in Escherichia coli strain XL-1 Blue
-
expression as His-tagged protein
-
expression of calmodulin-independent mutant MLCK1745 and of MLVKATPdel mutant in African green monkey kidney CV1 fibroblasts as V5-epitope- and His-tagged proteins
-
expression of calmodulin-independent mutant MLCK1745, lacking the C-terminal part including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, and of MLVKATPdel mutant with a defective ATP-binding site in bovine pulmonary artery endothelial cells BPAEC as V5-epitope-tagged proteins
-
expression of epitope-tagged endothelial isozyme in endothelial cells, expression of GST-fusion isozyme EC MLCK in bacteria, expression of endothelial iszyme EC MLCK and macrophage migration inhibition factor MIF in the yeast two-hybrid system, overview
-
expression of GFP-tagged enzyme in HeLa cells
-
gene MYLK, DNA and amino acid sequence determination and analysis of wild-type and single nucleotide mutants, genotyping
-
genes MYLK2 and MYLK3, DNA and amino acid sequence determination and analysis, expression analysis in heart, sequence comparisons
-
obscurin-associated kinase, DNA and amino acid sequence determination, sequence and expression analysis and comparison, both the mouse and human obscurinassociated kinase-like transcripts also share a conserved putative translation initiation site, overview. Expression in Escherichia coli strain BL21(DE3)
-
stable expression of a constructed Ca2+/calmodulin-dependent fluorescent biosensor MLCK in HEK-293 cells
-
DNA and amino acid sequence determination and analysis, expression analysis in cardiac smooth muscle, overview, expression of HA-tagged MLCK
expression of wild-type and dominant-negative MLCK in untransformed and Ras-transformed MCF-10A cells
-
gene Mlck, expression analysis, transgenic expression in mice bladder smooth muscle via adenoviral transfection
-
gene mylk1, located on chromosome 16, contains at least 31 exons, DNA and amino acid sequence determination and analysis, genetic structure and regulation, the serum response factor myocardin plays a central role in regulating mylk1 gene expression, a highly conserved CArG element within 130-kDa MLCK intron 1 is important for promoter activity binding activation factors like myocardin, homebox proteins, GATA family members, and the thyrotroph embryonic factor, regulation model, overview
-
sequence and expression analysis and comparison, both the mouse and human obscurinassociated kinase-like transcripts also share a conserved putative translation initiation site, overview
-
cDNA, expressed in COS cells
-
expression in A7r5 rat thoracic aorta smooth muscle cells as GFP-fusion protein
-
expression in COS cells; expression in COS-cells
-
expression in COS-7 african green monkey kidney cells
-
expression in Sf9 insect cells
-
expression in transgenic mice, expression in COS cells
-
expression of the long isozyme L-MLCK in murine NIH3T3 cells, expression of GFP-tagged wild-type and mutant isozymes in COS-7 cells and as His-tagged proteins in Escherichia coli strain BL21(DE3)
-
telokin, the carboxyl terminus of the smooth muscle myosin light chain kinase which is expressed as an independent protein
-
isolation of cDNA
sequence and expression analysis and comparison, overview
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
breast cancer cells transiently activate endothelial MLCK at tumor invasion sites
-
0.1 nM androgen R1881 causes a progressive decrease in MLCK mRNA levels which decreases by 70% and 90%, respectively, at 24 and 48 h
-
the expression of MLCK is increased in endobronchial biopsies from humans with mild asthma
-
10 ng/ml tumour necrosis factor-alpha causes a significant increase of myosin light chain kinase gene activity, tumour necrosis factor-alpha causes an increase in nuclear factor-kappaB to the nuclear factor-kappaB1 binding motif which presumably leads to the activation of MLCK promoter
-
Lactobacillus rhamnosus alleviates intestinal barrier dysfunction in part by increasing expression of myosin light-chain kinase in vivo
-
MLCK expression is significantly up-regulated after controlled cortical impact with a maximum 12 h post-injury
-
severe burn injury increases intestinal MLCK protein levels 3.6fold after 2h in animals resuscitated with normal saline
-
expression of myosin light chain kinase protein is rapidly increased by angiotensin II
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
A27F/A29R/A31R/A32F
-
mutant enzyme with defective ATP binding site, mutant leads to nearly unaltered MLC phosphorylation activity and altered morphology in transfected CV1 cells
A983P
-
dramatic increase in Ca2+ required for half-maximal activity
A986P
-
significantly increase in Ca2+ required for half-maximal activity, slightly decreased KM for regulatory light chain
additional information
-
deletion of DRFXXL motifs leads to a worse binding to actin filaments especially in the presence of Mg2+
additional information
-
downregulation of MLCK in cultured smooth muscle cells reveals that MLC20 phosphorylation is not obligatory for the smooth muscle to contract, overview
H21P
-
naturally occuring single nucleotide polymorphism increasing the risk for acute lung injury within 5 das of traumatic insult
additional information
silencing of MLCK1 via specific siRNA increases transepithelial resistance in Caco-2 cells
additional information
-
construction of an enzyme mutant MLCK1745 which lacks the C-terminal amino acids including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, the MLCK1745 mutation leads to increased growth rates of transfected CV1 fibroblasts and an increase in basal level MLC phosphorylation compared to untransfected cells, stress fiber formation is unaffected by the mutation
additional information
-
construction of an enzyme mutant MLCK1745 which lacks the C-terminal amino acids including the autoinhibitory domain, and the myosin- and the calmodulin-binding sites, the MLCK1745 mutant increases the TNFalpha response to upregulate NFkappaB activity in transfected endothelial cells, MLCKATPdel mutant enzyme with defective ATP binding site inhibits TNFalpha-induced NFkappaB-activation activity in transfected endothelial cells
additional information
-
knockdown of cardiac-MLCK by specific siRNAs decreases cardiac-MLCK phosphorylation and impairs epinephrine-induced activation of sarcomere reassembly, knockdown of zcardiac-MLCK expression using morpholino antisense oligonucleotides results in dilated cardiac ventricles and immature sarcomere structures
additional information
expression of a dominant-negative mutant of MLCK inhibits the TRPC5 channel activity, revealing an essential role of MLCK in maintaining TRPC5 channel activity; expression of a dominant-negative mutant of MLCK inhibits the TRPC5 channel activity, revealing an essential role of MLCK in maintaining TRPC5 channel activity
additional information
-
single nucleotide polymorphisms are involved in the risk of sepsis-related acute lung injury and asthma, haplotype analysis, overview
additional information
-
vascular endothelial overexpression of the human nonmuscle isozyme of myosin light chain kinase-2 renders the transgenic mice susceptible to lipopolysaccharide-induced or mechanical-induced inflammatory lung injury, the homozygous mice show reduced fecundity and litter survival until weaning, as well as initially delayed growth compared to wild-type mice, phenotype and modeling, overview
P147S
-
naturally occuring single nucleotide polymorphism increasing the risk for acute lung injury within 5 das of traumatic insult
additional information
-
construction of eMLCK or smMLCK knockout mutant mice by genetic or chemical methods, overview
additional information
-
construction of a dominant-negative MLCK mutant
additional information
-
long isozyme MLCK-210-deficient mice display a significantly improved survival with a greatly attenuated microvascular hyperpermeability response to albumin and fluid
additional information
-
generation of tamoxifen-inducible and smooth muscle-specific MLCK knockout mutant mice with loss of function and impaired smooth muscle contraction, severe gut dysmotility, phenotype, overview
additional information
-
construction of 1-AMPK knock-out mice
M968P
-
10% Ca2+/calmodulin independent activity of total activity, decreased KM for regulatory light chain
additional information
-
serial carboxy-terminal deletions of the regulatory and catalytic domains are constructed and expressed in COS cells. The truncated kinases have no detectable myosin light chain kinase activity
additional information
-
construction of truncation mutants of long and short MLCKs
additional information
-
construction of transgenic mice that express an skeletal muscle-MLCK, i.e. skMLCK, CaM biosensor in skeletal muscle to determine whether skMLCK or calmodulin is limiting to twitch force potentiation. Three transgenic mouse lines exhibited up to 22-fold increases in skMLCK protein expression in fast-twitch extensor digitorum longus muscle containing type IIa and IIb fibers, with comparable expressions in slow-twitch soleus muscle containing type I and IIa fibers, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
therapeutic inhibition via specific chemical inhibitors of endothelial isozyme, i.e. 11-(3-chloro-6-immino-6H-pyridazin-1-yl)-undecanoic acid (6-phenyl-pyridazin-3-yl)-amide, represents an important new target for the treatment of inflammatory lung disease with less side effects than other therapies
medicine
-
long isozyme MLCK-210 is a potential therapeutic target in the treatment of burn edema