Information on EC 2.7.11.17 - Ca2+/calmodulin-dependent protein kinase and Organism(s) Homo sapiens and UniProt Accession Q13554

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Homo sapiens
UNIPROT: Q13554


The taxonomic range for the selected organisms is: Homo sapiens

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.11.17
-
RECOMMENDED NAME
GeneOntology No.
Ca2+/calmodulin-dependent protein kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a protein = ADP + a phosphoprotein
show the reaction diagram
sequential reaction mechanism in which all substrates bind to enzyme before any product is released. Binding of phospholamban to the enzyme decreases the affinity of the enzyme toward ATP
-
ATP + a protein = ADP + a phosphoprotein
show the reaction diagram
sequential reaction mechanism in which all substrates bind to enzyme before any product is released. Binding of phospholamban to the enzyme decreases the affinity of the enzyme toward ATP
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (Ca2+/calmodulin-dependent)
Requires calmodulin and Ca2+ for activity. A wide range of proteins can act as acceptor, including vimentin, synapsin, glycogen synthase, myosin light chains and the microtubule-associated tau protein. Not identical with EC 2.7.11.18 (myosin-light-chain kinase) or EC 2.7.11.26 (tau-protein kinase).
CAS REGISTRY NUMBER
COMMENTARY hide
141467-21-2
-
93229-57-3
-
97350-82-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
-
in human hypertrophy, both CaMKII and PKA functionally regulate RyR2 and may induce SR Ca2+ leak. In the transition from hypertrophy to heart failure, the diastolic Ca2+ leak increases and disturbed Ca2+ cycling occurs. This is associated with an increase in CaMKII- but not PKA-dependent RyR2 phosphorylation. PKA-dependent RyR2 phosphorylation is not increased in heart failure and is independent of previous beta-blocker treatment. In heart failure, CaMKII inhibition but not inhibition of PKA yields a reduction of the SR Ca2+ leak. PKA inhibition further reduced SR Ca2+ load and systolic Ca2+ transients. CaMKII inhibition may thus reflect a promising therapeutic target for the treatment of arrhythmias and contractile dysfunction
physiological function
additional information
-
CAMKIV structure-function analysis, pH dependence, molecular dynamics simulations and three-dimensional structure analysis, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
ADP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-phospho-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
show the reaction diagram
-
biotinylated phospholamban Thr17 peptide substrate
-
-
?
ATP + phospholamban
ADP + phosphorylated phospholamban
show the reaction diagram
-
-
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + Akt
ADP + phosphorylated Akt
show the reaction diagram
-
substrate of CaM-KII, phosphorylation at Thr308
-
-
?
ATP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
ADP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-phospho-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
show the reaction diagram
-
biotinylated phospholamban Thr17 peptide substrate
-
-
?
ATP + Blc10
ADP + phosphorylated Blc10
show the reaction diagram
ATP + Ca2+/calmodulin-dependent protein kinase I
ADP + phosphorylated Ca2+/calmodulin-dependent protein kinase I
show the reaction diagram
ATP + Ca2+/calmodulin-dependent protein kinase IV
ADP + phosphorylated Ca2+/calmodulin-dependent protein kinase IV
show the reaction diagram
ATP + Cabin1
ADP + phosphorylated Cabin1
show the reaction diagram
ATP + cAMP-response element binding protein
ADP + phosphorylated cAMP-response element binding protein
show the reaction diagram
-
i.e. CREB, phosphorylation by CaMKII and CaMKIV at Ser133, and phosphorylation by CaMKII at Ser142
-
-
?
ATP + CARMA1
ADP + phosphorylated CARMA1
show the reaction diagram
ATP + CREB
ADP + phosphorylated CREB
show the reaction diagram
-
i.e. cAMP-response element binding protein, substrate of CaM-KI, phosphorylation at Ser133
-
-
?
ATP + eukaryotic translation initiation factor 4B
ADP + phosphorylated eukaryotic translation initiation factor 4B
show the reaction diagram
-
-
-
?
ATP + glutamate receptor subunit GluR1
ADP + phosphorylated glutamate receptor subunit GluR1
show the reaction diagram
ATP + glutamate receptor subunit NR2B
ADP + phosphorylated glutamate receptor subunit NR2B
show the reaction diagram
ATP + histone deacetylase 5
ADP + phosphorylated histone deacetylase 5
show the reaction diagram
-
-
-
-
?
ATP + I-kappaB
ADP + phosphorylated I-kappaB
show the reaction diagram
ATP + L-type Ca2+ channel
ADP + phosphorylated L-type Ca2+ channel
show the reaction diagram
ATP + NADPH oxidase 5
ADP + phosphoraletd NADPH oxidase 5
show the reaction diagram
ATP + phospholamban
ADP + phosphorylated phospholamban
show the reaction diagram
-
-
-
-
?
ATP + protein PLB
ADP + phosphorylated protein PLB
show the reaction diagram
-
a regulatory protein in Ca2+ signaling
-
-
?
ATP + protein RyR
ADP + phosphorylated protein RyR
show the reaction diagram
-
a regulatory protein in Ca2+ signaling
-
-
?
ATP + PSD-95
ADP + phosphorylated PSD-95
show the reaction diagram
ATP + ryanodine receptor type 2
ADP + phosphorylated ryanodine receptor type 2
show the reaction diagram
-
i.e. RyR2
-
-
?
ATP + Stargazin
ADP + phosphorylated Stargazin
show the reaction diagram
ATP + STAT3
ADP + phosphorylated STAT3
show the reaction diagram
-
-
-
?
ATP + stress-induced phosphoprotein 1
ADP + phosphorylated stress-induced phosphoprotein 1
show the reaction diagram
phosphorylated on Ser198
-
-
?
ATP + synapsin I
ADP + phosphorylated synapsin I
show the reaction diagram
-
substrate of CaM-KI
-
-
?
ATP + synapsin I
ADP + phosphosynapsin I
show the reaction diagram
-
-
-
-
?
ATP + synapsin II
ADP + phosphorylated synapsin II
show the reaction diagram
-
substrate of CaM-KI
-
-
?
ATP + synapsin-1
ADP + phopshorylated synapsin-1
show the reaction diagram
-
-
-
-
?
ATP + synthide-2
ADP + phorphorylated synthide-2
show the reaction diagram
-
-
-
?
ATP + tau peptide 408-420
ADP + phosphorylated tau peptide 408-420
show the reaction diagram
-
recombinant substrate expressed in Escherichia coli, phosphorylation at Ser416 by CaM kinase II, no activity with the peptide by CDK5, EC 2.7.11.22, and casein kinase II, EC 2.7.11.26
-
-
?
ATP + tau protein
ADP + phosphorylated tau protein
show the reaction diagram
ATP + type alpha cytosolic phospholipase A2
ADP + phosphorylated type alpha cytosolic phospholipase A2
show the reaction diagram
-
CaMK regulates the type alpha cytosolic phospholipase A2 via phosphorylation at Ser515
-
-
?
ATP + vimentin
ADP + phosphorylated vimentin
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + phospholamban
ADP + phosphorylated phospholamban
show the reaction diagram
-
-
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + Blc10
ADP + phosphorylated Blc10
show the reaction diagram
-
Bcl10 is essential for antigen receptor-induced NF-kappaB activation, interleukin-2 production, and T-cell proliferation but is not required for TCR-induced tyrosine phosphorylation, calcium flux, or extracellular signal-regulated kinase activation
-
-
?
ATP + Ca2+/calmodulin-dependent protein kinase I
ADP + phosphorylated Ca2+/calmodulin-dependent protein kinase I
show the reaction diagram
Q96RR4
the enzyme is a member of the CaMK cascade that mediates the response to intracellular Ca2+ elevation. Ca2+/calmodulin-dependent protein kinase kinase phosphorylates and activates Ca2+/calmodulin-dependent protein kinase I and Ca2+/calmodulin-dependent protein kinase IV, which directly activate transcription factors
-
-
?
ATP + Ca2+/calmodulin-dependent protein kinase IV
ADP + phosphorylated Ca2+/calmodulin-dependent protein kinase IV
show the reaction diagram
Q96RR4
the enzyme is a member of the CaMK cascade that mediates the response to intracellular Ca2+ elevation. Ca2+/calmodulin-dependent protein kinase kinase phosphorylates and activates Ca2+/calmodulin-dependent protein kinase I and Ca2+/calmodulin-dependent protein kinase IV, which directly activate transcription factors
-
-
?
ATP + Cabin1
ADP + phosphorylated Cabin1
show the reaction diagram
-
a transcriptional corepressor of myocyte enhancer factor 2, phosphorylation by CaMKIV creates a docking site for protein 14-3-3, which causes nuclear export, CaMKIV regulates nuclear export of Cabin1 during Ca2+-dependent T-cell activation, regulation overview
-
-
?
ATP + cAMP-response element binding protein
ADP + phosphorylated cAMP-response element binding protein
show the reaction diagram
-
i.e. CREB, phosphorylation by CaMKII and CaMKIV at Ser133, and phosphorylation by CaMKII at Ser142
-
-
?
ATP + CARMA1
ADP + phosphorylated CARMA1
show the reaction diagram
-
CaMKII is a modulator of CARMA1-mediated NF-kappaB activation, overview
-
-
?
ATP + glutamate receptor subunit GluR1
ADP + phosphorylated glutamate receptor subunit GluR1
show the reaction diagram
-
low activity, CaMKII, membrane localization of GluR1 restricts its phosphorylation
-
-
?
ATP + glutamate receptor subunit NR2B
ADP + phosphorylated glutamate receptor subunit NR2B
show the reaction diagram
-
low activity, CaMKII
-
-
?
ATP + I-kappaB
ADP + phosphorylated I-kappaB
show the reaction diagram
-
substrate of CaM-KII in T-lymphocytes and neurons, phosphorylation leads to activation of I-kappa B, which mediates activation of NF-kappaB
-
-
?
ATP + L-type Ca2+ channel
ADP + phosphorylated L-type Ca2+ channel
show the reaction diagram
-
inactivation, leading to increased Ca2+ channel open probability and increased arrhytmias
-
-
?
ATP + NADPH oxidase 5
ADP + phosphoraletd NADPH oxidase 5
show the reaction diagram
-
the enzyme mediates the phosphorylation and activation of NADPH oxidase 5. The ability of Ca2+/calmodulin-dependent protein kinase to regulate NADPH oxidase 5 activity may be significant in the regulation of production of reactive oxygen species that occurs downstream of calcium-mobilizing agonists such as angiotensin II. Of these phosphorylation sites, mutation of only Ser475 to alanine prevents Ca2+/calmodulin-dependent protein kinase-induced increases in activity of NADPH oxidase 5
-
-
?
ATP + phospholamban
ADP + phosphorylated phospholamban
show the reaction diagram
-
-
-
-
?
ATP + protein PLB
ADP + phosphorylated protein PLB
show the reaction diagram
-
a regulatory protein in Ca2+ signaling
-
-
?
ATP + protein RyR
ADP + phosphorylated protein RyR
show the reaction diagram
-
a regulatory protein in Ca2+ signaling
-
-
?
ATP + PSD-95
ADP + phosphorylated PSD-95
show the reaction diagram
-
high activity, CaMKII, smaller synapses show greater variability in PSD-95 phosphorylation
-
-
?
ATP + ryanodine receptor type 2
ADP + phosphorylated ryanodine receptor type 2
show the reaction diagram
-
i.e. RyR2
-
-
?
ATP + Stargazin
ADP + phosphorylated Stargazin
show the reaction diagram
-
high activity, CaMKII, phosphatases limit phosphorylation of stargazin
-
-
?
ATP + synapsin-1
ADP + phopshorylated synapsin-1
show the reaction diagram
-
-
-
-
?
ATP + tau protein
ADP + phosphorylated tau protein
show the reaction diagram
-
phosphorylation at Ser416 by CaM kinase II is high in early stages of brain development, CaM kinase II is involved in the accumulation of tau in neuronal soma in Alzheimer's disease brain
-
-
?
ATP + vimentin
ADP + phosphorylated vimentin
show the reaction diagram
-
specific phosphorylation by CaMKII at Ser82
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
Ca2+/calmodulin-dependent
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
-
product inhibition is best fit with an ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus ATP, uncompetitive inhibition versus phospholamban, isoenzyme CaMK IIbeta
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
-
product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus phospholamban and ATP, isoenzyme CaMK IIbeta
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
-
-
2-[4-[6-(quinolin-8-yloxy)pyrimidin-4-yl]piperazin-1-yl]benzonitrile
-
-
-
2-[N-(2-hydroxyethyl)-N-(4-methoxybenzenesulfonyl)]amino-N-(4-chlorocinnamyl)-N-methylbenzylamine
-
KN-93
3-([6-(quinolin-8-yloxy)pyrimidin-4-yl]oxy)aniline
-
-
-
7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylate
i.e. STO-609. The CaMKKbeta residue Pro274, which replaces the conserved acidic residue of other protein kinases, is an important determinant for the selective inhibition by STO-609. ATP-competitive inhibition mechanism
AC3-I
-
specific CaMKII inhibitor
AIP
-
specific CaMKII inhibitor
autocamide-2-related inhibitory peptide
-
i.e. AIP, used in its myristoylated form
-
autocamtide-2 related inhibitory peptide
-
-
-
autocamtide-2-related inhibitory peptide
-
specific and potent CaMKII inhibitor
-
BAPTA-AM
-
-
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
Calmidazolium
-
calmodulin antagonist
CaM-KIIN
-
noncompetitive inhibitor
-
CaMK phosphatase
-
specifically dephosphorylates and regulates activity
-
CaMKI (294-321)
-
specific CaMKI inhibitor
-
CaMKII (273-302)
-
specific CaMKII inhibitor
-
CaMKII (281-309)
-
specific CaMKII inhibitor
-
CaMKIIN
-
brain-specific CaMKII inhibitor
-
CaMKP phosphatase-N
-
specifically dephosphorylates and regulates activity
-
CN-21
-
specific CaMKII inhibitor
-
imatinib
-
downregulates CaMKIIgamma autophosphorylation/activation
K252a
-
inhibits CaM-KII
KN-62
KN-93
KN62
-
inhibits CaMKII
methyl-beta-cyclodextrin
-
-
N-(4-methoxyphenyl)-6-(piperazin-1-yl)pyrimidin-4-amine
-
-
pea protein peptides
-
purified positively charged peptides produced from pea cellular proteins by alkaline protease, i.e. alcalase, are inhibitory for CaMKII in a competitive manner, especially those with a high content of lysine and arginine, which act more in a mixed inhibition type, amino acid compositions of the peptides and IC50 of peptide fractions, overview
-
PEP-19
-
specific CaMKII inhibitor
-
peptide AC3-I
-
i.e. autocamtide-2 inhibitory peptide, inhibits CaMKII
peptide AIP
-
i.e. autocamtide-2-related inhibitory peptide, comprising 17 amino acids, a CaMKII inhibitory peptide with the sequence KKALRRQEAVDAL
protein phosphatase 1
-
negative CaMK regulation by dephosphorylation
-
Protein phosphatase 2A
-
negative CaMK regulation by dephosphorylation
-
protein phosphatase 2B
-
calcineurin, negative CaMK regulation by dephosphorylation
-
protein phosphatase 2C
-
negative CaMK regulation by dephosphorylation
-
protein phosphatase M
-
negative CaMK regulation by dephosphorylation
-
STO-609
trifluoperazine dimaleate
-
calmodulin antagonist
W-13
-
calmodulin antagonist
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7-oxo-7H-benzimidazo[2,1-a]benz[de]isoquinoline-3-carboxylic acid
-
STO-609, triggers the increase of Ca2+ and activation of Ca2+/calmodulin-dependent protein kinase Ialpha
Ca2+/calmodulin-dependent protein kinase kinase
-
-
-
Calmodulin
ionomycin
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0259
ATP
-
pH 7.6, 25C, isoenzyme CaMK IIbeta
0.0158
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
-
pH 7.6, 25C, isoenzyme CaMK IIbeta
0.0071 - 0.0128
ATP
0.0064 - 0.0097
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
additional information
additional information
-
kinetics for binding of calmodulin
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8
ATP
-
pH 7.6, 25C, isoenzyme CaMK IIbeta
8
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
-
pH 7.6, 25C, isoenzyme CaMK IIbeta
1.2 - 9.9
ATP
1.2 - 9.9
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
inhibition kinetics for positively charged pea protein-derived peptides
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.104
2-[4-[6-(quinolin-8-yloxy)pyrimidin-4-yl]piperazin-1-yl]benzonitrile
Homo sapiens
-
HuH7 cells, Eagle's medium, pH not specified in the publication, 37C
-
0.116
3-([6-(quinolin-8-yloxy)pyrimidin-4-yl]oxy)aniline
Homo sapiens
-
HuH7 cells, Eagle's medium, pH not specified in the publication, 37C
-
0.02 - 0.5
7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylate
0.046
N-(4-methoxyphenyl)-6-(piperazin-1-yl)pyrimidin-4-amine
Homo sapiens
-
HuH7 cells, Eagle's medium, pH not specified in the publication, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.004 - 0.022
-
recombinant nonphosphorylated, calmodulin-binding-residue mutants
0.035
purified recombinant CaM-K1delta
0.98
purified recombinant CaM-K1delta phosphorylated by CaM-KKalpha
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
-
assay at
7
-
assay at
7.4
-
assay at
7.6
-
assay at
8
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 11.5
-
within the pH range, CAMKIV maintains both its secondary and tertiary structures, along with its function, whereas significant aggregation is observed at acidic pH 2.0-4.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
22
-
assay at room temperature
24
-
assay at
25
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
very low level
Manually annotated by BRENDA team
-
moderate expression
Manually annotated by BRENDA team
-
very low level
Manually annotated by BRENDA team
-
the enzyme is absent from primary human B lymphocytes but is expressed in Epstein-Barr virus-transformed B-lymphoblastoid cell lines, suggesting that expression of the kinase can be upregulated by an EBV gene products
Manually annotated by BRENDA team
-
from heart failure
Manually annotated by BRENDA team
-
Jurkat cell
Manually annotated by BRENDA team
-
CaM-KI
Manually annotated by BRENDA team
-
breast cancer cell line
Manually annotated by BRENDA team
low expression level of CaM-K1delta
Manually annotated by BRENDA team
low expression level of CaM-K1delta
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
neuron
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
isozyme CaMKI
41340
-
-
51925
-
x * 51925, calculation from nucleotide sequence
60000
-
isozyme CaMKIV
550000
-
isozyme CaMKII
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 51925, calculation from nucleotide sequence
monomer
multimer
oligomer
-
isozyme CaMKII
additional information
-
CAMKIV structure-function analysis, pH dependence, molecular dynamics simulations and three-dimensional structure analysis, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method at 20C, crystal structure of the catalytic kinase domain of the human CaMKKbeta isoform complexed with its selective inhibitor 7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylate (i.e. STO-609). The crystal belongs to the primitive orthorhombic space group P2(1)2(1)2(1) with unit cell constants of a = 69.4 A, b = 77.2 A, c = 84.3 A
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
CaMKIIalpha clusters associated with lipid raft fraction are resistant to treatment with methyl-beta-cyclodextrin and subsequent cold Triton X-100, which suggests the stabilization of CaMKIIalpha cluster-associated lipid rafts
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant FLAG-tagged CaMKIV from 293A cells
-
recombinant functionally active CAMKIV kinase domain
-
recombinant GST-tagged wild-type and mutant CaM-K1delta from Escherichia coli by glutathione affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CaM-K1delta, DNA and amino acid sequence determination and analysis, expression of GST-tagged wild-type and mutant enzyme in Escherichia coli strain JM109, transient expression of HA-tagged CaM-K1delta 1-296 Ca2+/calmodulin-independent mutant in HeLa and COS-7 cells
CaMKIgamma, DNA and amino acid sequence determination and analysis, expression in COS-7 cells
cloning and recombinant expression of functionally active CAMKIV kinase domain
-
coexpression of GST-tagged enzyme with GST-tagged Cav2.1 channels in transfected tsA-201 cells and GST affinity pulldown assay, recombinant expression of MBP-fusion enzyme in Escherichia coli strain BL21
-
expressed in a wheat cell-free system
expressed in Escherichia coli
-
expressed in GT1-7 cells
-
expressed in the LXSN retroviral expression vector M28248
-
expression in Escherichia coli
expression in Jurkat cells, co-expression with human CARMA1, T-cell receptor engagement redistributes CaMKII to the immune synapse in an activation-dependent manner
-
expression of CaMKII in HEK-293 cells, and expression of CaMKII in cultured neurons using lentiviral transfection, co-expression of protein substrates
-
expression of FLAG-tagged CaMKIV in 293A cells
-
functional cytoplasmic co-expression of wild-type CaMKII with vimentin-NR2B, a construct of a vimentin peptide containing Ser82 fused to the NMDA receptor 2B subunit, an interaction partner of CaMKII, in HEK-293 cells, translocation of the Vin-NR2B construct to the membrane is regulated by lipid rafts resulting in reduced phosphorylation by CaMKII
-
isolation and sequencing of cDNA
-
recombinant expression in TsA-201 cells, the recombinant enzyme interacts and co-localizes with the presynaptic N-type voltage-dependent calcium channel (Cav2.2) and induces in TsA-201 cell line an increase of calcium current mediated by this channel
-
transient co-expression of CaMKIV, MEF-2, and Cabin1 in murine T-cell hybridoma DO11.10, MEF-2 activation of gene transcription is inhibited by Cabin1, the inhibition is reversed by CaMKIV
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F320D/N321D
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site-directed mutagenesis, the mutation changes the sequence FNDD to DDDD, the mutant shows no binding of calmodulin and increased activity in nonphosphorylated status compared to the wild-type enzyme
I205K
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site-directed mutagenesis, the mutant CaMKII shows reduced activity with vimentin compared to the wild-type enzyme
K75E
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site-directed mutagenesis, catalytically inactive CaMKIV mutant
P274E
the P274E mutation significantly reduces the inhibitory effect of 7H-benzimidazo(2,1-a)benz(de)isoquinoline-7-one-3-carboxylate (i.e. STO-609) on the isoenzyme CaMKKbeta
T180A
site-directed mutagenesis, the mutant enzyme shows highly reduced activity compared to the wild-type enzyme, the mutant is phosphorylated and activated by CaM-KKalpha
T200A
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site-directed mutagenesis, the mutant CaMKIV is not phosphorylated by CaMKKbeta and is thus incapable of developing autonomous activity and control gene transcription, the same effect occurs due to combination of T200A with calmodulin binding site mutations, overview
T287D
constitutively active CaMKIId mutant
T287D/K43M
kinase dead CaMKIId mutant
additional information
APPLICATION
ORGANISM
UNIPROT
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LITERATURE
medicine