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Information on EC 2.7.11.14 - rhodopsin kinase and Organism(s) Drosophila melanogaster and UniProt Accession P32865

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EC Tree
IUBMB Comments
Requires G-protein for activation and therefore belongs to the family of G-protein-dependent receptor kinases (GRKs). Acts on the bleached or activated form of rhodopsin; also phosphorylates the beta-adrenergic receptor, but more slowly. Does not act on casein, histones or phosphvitin. Inhibited by Zn2+ and digitonin (cf. EC 2.7.11.15, beta-adrenergic-receptor kinase and EC 2.7.11.16, G-protein-coupled receptor kinase).
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This record set is specific for:
Drosophila melanogaster
UNIPROT: P32865
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Word Map
The taxonomic range for the selected organisms is: Drosophila melanogaster
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
rk, rhodopsin kinase, g protein-coupled receptor kinase, g-protein receptor kinase, g-protein coupled receptor kinase, g protein-coupled receptor kinase 1, g-protein coupled receptor kinase 1, gprk1, g-protein-coupled receptor kinase 1, g-protein receptor kinase-1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G protein-coupled receptor kinase 1
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G protein-coupled receptor kinase 1
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G-protein coupled receptor kinase
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GPCR kinase 1
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kinase (phosphorylating), opsin
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kinase (phosphorylating), rhodopsin
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opsin kinase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
ATP:rhodopsin phosphotransferase
Requires G-protein for activation and therefore belongs to the family of G-protein-dependent receptor kinases (GRKs). Acts on the bleached or activated form of rhodopsin; also phosphorylates the beta-adrenergic receptor, but more slowly. Does not act on casein, histones or phosphvitin. Inhibited by Zn2+ and digitonin (cf. EC 2.7.11.15, beta-adrenergic-receptor kinase and EC 2.7.11.16, G-protein-coupled receptor kinase).
CAS REGISTRY NUMBER
COMMENTARY hide
54004-64-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + rhodopsin 1
ADP + phosphorhodopsin 1
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + rhodopsin 1
ADP + phosphorhodopsin 1
show the reaction diagram
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-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
G-protein
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dependent on
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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assay at dark
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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most GPRK1 in the eye
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GPRK1_DROME
700
0
80566
Swiss-Prot
other Location (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K220R
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site-directed mutagenesis, ATP binding site mutation, inactive mutant
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression of myc-tagged wild-type and K220R mutant enzyme fused to heat shock inducible promotors in photoreceptor cells of flies, cells overexpressing the wild-type enzyme show increased phosphorhodopsin content after heat shock activation
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cassill, J.A.; Whitney, M.; Joazeiro, C.A.; Becker, A.; Zuker, C.S.
Isolation of Drosophila genes encoding G protein-coupled receptor kinases
Proc. Natl. Acad. Sci. USA
88
11067-11070
1991
Drosophila melanogaster (P32865)
Manually annotated by BRENDA team
Lee, S.J.; Xu, H.; Montell, C.
Rhodopsin kinase activity modulates the amplitude of the visual response in Drosophila
Proc. Natl. Acad. Sci. USA
101
11874-11879
2004
Drosophila melanogaster
Manually annotated by BRENDA team