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Information on EC 2.7.11.1 - non-specific serine/threonine protein kinase and Organism(s) Bos taurus and UniProt Accession Q01314

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IUBMB Comments
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
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Select one or more organisms in this record: ?
This record set is specific for:
Bos taurus
UNIPROT: Q01314
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
protein kinase a, 14-3-3, camkii, p-akt, lrrk2, c-jun n-terminal kinase, serine/threonine kinase, raf-1, protein kinase b, gsk-3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
RAC-alpha serine/threonine kinase
-
A-kinase
-
-
-
-
A-T, mutated
-
-
-
-
A-T, mutated homolog
-
-
-
-
AMH type II receptor
-
-
-
-
Anti-sigma B factor rsbT
-
-
-
-
AP50 kinase
-
-
-
-
Ataxia telangiectasia mutated
-
-
-
-
Ataxia telangiectasia mutated homolog
-
-
-
-
ATP-protein transphosphorylase
-
-
-
-
betaIIPKC
-
-
-
-
Breast-tumor-amplified kinase
-
-
-
-
Calcium-dependent protein kinase C
-
-
-
-
Calcium/phospholipid-dependent protein kinase
-
-
-
-
cAMP-dependent protein kinase A
-
-
-
-
casein kinase
-
-
-
-
casein kinase 2
-
-
-
-
casein kinase I
-
-
-
-
casein kinase I, alpha isoform
-
casein kinase I, beta isoform
-
casein kinase II
casein kinase-2
-
-
-
-
CDC25 suppressing protein kinase
-
-
-
-
Cell division cycle 2-like
-
-
-
-
CK-2
-
-
-
-
CK2
-
-
-
-
CK2-alpha
-
-
-
-
CKI
-
-
-
-
CKI-alpha
-
CKI-beta
-
CKII
-
-
-
-
CLP-36 interacting kinase
-
-
-
-
cyclic monophosphate-dependent protein kinase
-
-
-
-
cyclic nucleotide-dependent protein kinase
-
-
-
-
cyclin-dependent kinase
-
-
-
-
cytidine 3',5'-cyclic monophosphate-responsive protein kinase
-
-
-
-
DNA-PKcs
-
-
-
-
DNPK1
-
-
-
-
dSTPK61
-
-
-
-
G11 protein
-
-
-
-
glycogen synthase kinase-3beta
-
-
hARK1
-
-
-
-
histone kinase
-
-
-
-
hPDK1
-
-
-
-
Hpr kinase
-
-
-
-
hPSK
-
-
-
-
HSPK 21
-
-
-
-
HSPK 36
-
-
-
-
hydroxyalkyl-protein kinase
-
-
-
-
Kinase interacting with stathmin
-
-
-
-
kinase, casein (phosphorylating)
-
-
-
-
kinase, protamine (phosphorylating)
-
-
-
-
kinase, protein (phosphorylating)
-
-
-
-
kinase, protein, A (phosphorylating)
-
-
-
-
kinase, protein, C (phosphorylating)
-
-
-
-
Lymphocyte-oriented kinase
-
-
-
-
M phase-specific cdc2 kinase
-
-
-
-
mitogen-activated S6 kinase
-
-
-
-
mPDK1
-
-
-
-
MPSK
-
-
-
-
Myristoylated and palmitoylated serine-threonine kinase
-
-
-
-
NY-REN-55 antigen
-
-
-
-
P-CIP2
-
-
-
-
P460
-
-
-
-
p46Eg265
-
-
-
-
p46XlEg22
-
-
-
-
p82 kinase
-
-
-
-
pEg2
-
-
-
-
phosphorylase b kinase kinase
-
-
-
-
Phototropin
-
-
-
-
Pim-2h
-
-
-
-
PkB kinase
-
-
-
-
PKN
-
-
pp39-mos
-
-
-
-
Pre-mRNA protein kinase
-
-
-
-
protamine kinase
-
-
-
-
protein glutamyl kinase
-
-
-
-
protein kinase (phosphorylating)
-
-
-
-
protein kinase A
-
-
Protein kinase B kinase
-
-
-
-
protein kinase CK2
-
-
-
-
Protein kinase Krct
-
-
-
-
Protein kinase MST
-
-
-
-
protein kinase p58
-
-
-
-
Protein kinase PKL12
-
-
-
-
protein phosphokinase
-
-
-
-
protein serine kinase
-
-
-
-
protein serine-threonine kinase
-
-
-
-
protein-aspartyl kinase
-
-
-
-
protein-cysteine kinase
-
-
-
-
protein-serine kinase
-
-
-
-
protein-serine/threonine kinase
-
-
-
-
PRP4 kinase
-
-
-
-
PRP4 pre-mRNA processing factor 4 homolog
-
-
-
-
PSK-H1
-
-
-
-
Raf kinase
-
-
-
-
Raf-1
-
-
-
-
ratAurA
-
-
-
-
ribosomal protein S6 kinase II
-
-
-
-
ribosomal S6 protein kinase
-
-
-
-
RP1 protein
-
-
-
-
S-receptor kinase
-
-
-
-
serine kinase
-
-
-
-
serine protein kinase
-
-
-
-
serine(threonine) protein kinase
-
-
-
-
serine-specific protein kinase
-
-
-
-
serine-threonine kinase
-
-
serine/threonine kinase
-
-
-
-
Serine/threonine kinase 15
-
-
-
-
Serine/threonine kinase Ayk1
-
-
-
-
serine/threonine protein kinase
-
-
-
-
Serine/threonine-protein kinase NRK2
-
-
-
-
Serine/threonine-protein kinase NYD-SPK
-
-
-
-
SRK
-
-
-
-
Ste20-like kinase
-
-
-
-
STE20-like kinase MST1
-
-
-
-
STE20-like kinase MST2
-
-
-
-
STE20-like kinase MST3
-
-
-
-
Switch protein/serine kinase
-
-
-
-
T-antigen kinase
-
-
-
-
threonine-specific protein kinase
-
-
-
-
Twitchin kinase
-
-
-
-
type-2 casein kinase
-
-
-
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (non-specific)
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
CAS REGISTRY NUMBER
COMMENTARY hide
191808-15-8
phosphoinositide dependent protein kinase 1
340830-03-7
-
37278-10-7
-
377752-08-4
ribosomal protein S6 kinase 2
389133-24-8
ribosomal S6 kinase 3
52660-18-1
casein kinase, protein kinase CK2
9026-43-1
this CAS Reg. No. encompasses a great variety of protein kinases including the serine/threonine specific kinases
90698-26-3
ribosomal protein S6 kinase 1
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + Ac-LRRASLG
ADP + Ac-LRRApSLG
show the reaction diagram
-
an efficient fluorescence assay for serine/threonine kinases using a peptide array is developed. Assay is based on chemical reactions specific to phosphoserine and phosphothreonine residues, that is, base-mediated beta-elimination of the phosphate group and subsequent Michael addition of a thiol-containing fluorescent reagent. This procedure enables the covalent introduction of a fluorescent moiety into the phosphorylated peptide. Novel fluorescent reagents are designed for this purpose and synthesized. As a model peptide, a well-known PKA consensus sequence Kemptide 49 is chosen
-
-
?
ATP + bovine serum albumin
?
show the reaction diagram
ATP + casein
?
show the reaction diagram
ATP + eukaryotic protein synthesis initiation factor
?
show the reaction diagram
-
i.e. eIF-4E, 1 mol phosphate per mol eIF-4E
-
-
?
ATP + glycogen synthase
?
show the reaction diagram
ATP + histone H1
?
show the reaction diagram
ATP + histone IIB
?
show the reaction diagram
ATP + protamine
ADP + O-phosphoprotamine
show the reaction diagram
ATP + several 40S ribosomal polypeptides
?
show the reaction diagram
-
e.g. S6, 2.5 mol phosphate per mol S6
-
-
?
ATP + synthetic peptide
?
show the reaction diagram
-
Arg-Arg-Leu-Ser-Ser-Leu-Arg-Ala
-
-
?
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
casein + ATP
phosphorylated casein + ADP
show the reaction diagram
-
-
-
?
RRRDDDSDDD + ATP
? + ADP
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
-
substrate of GSK3beta in brain
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
can replace Mg2+ with 20% efficiency
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Catalytic subunit of protein phosphatase 2A
-
O-phosphoprotamine
-
bovine serum albumin, not protamine as substrate
Protein phosphatase 1b
-
weak
-
Protein phosphatase 2A
-
spermine
-
bovine serum albumin, not protamine as substrate
staurosporine
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3',5'-AMP
arachidonic acid
-
activates in vitro, binds at the autoinhibitory site at the C-terminus
protein 14-3-3
-
a dimeric scaffold protein, protein is absolutely required for connection by simultaneous binding of glycogen synthase kinase-3 beta to tau within a brain microtubule-associated tau phosphorylation complex
-
RhoA
-
small GTPase, binds to the ACC domain and activates isozymes PKNalpha and PRK2/PKNgamma/PAK-2
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00025
staurosporine
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
staurosporine
Bos taurus
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17.38
-
-
3 - 5
substrate: RRRDDDSDDD
5.11
-
-
7.53
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7 - 9
-
broad, protamine as substrate
6 - 9
-
-
6.1 - 8.2
-
protamine as substrate
6.7
-
bovine serum albumin as substrate
7 - 7.6
-
eIF-4E as substrate
7.4
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 8.8
-
about 85% of maximal activity at pH 5.8 and about 80% of maximal activity at pH 8.8, protamine as substrate
6.6 - 6.8
-
about half-maximal activity at pH 6.6 and pH 6.8, bovine serum albumin as substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
isozymes show different tissue distribution
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
microsomal kinase is a form of the cytosolic kinase
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AKT1_BOVIN
480
0
55748
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
gel filtration
45000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 45000, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
diluted solutions are unstable
-
repeated freeze-thawing inactivates
-
Triton X-100 stabilizes during freeze-thawing procedures
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, at least 6 months
-
-70°C, in 0.025 M Tris-HCl, pH 7.3, 10% glycerol, 1 mM EDTA, 1 mM benzamidine, 0.1 mM PMSF, 14 mM 2-mercaptoethanol, 0.1% Triton X-100, 12 months
-
0-4°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, up to 3 weeks
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cytosolic
-
isozyme PKNalpha from brain membrane
-
microsomal
-
mitochondrial kinase
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of glycogen synthase kinase-3 beta, tau, and 14-3-3 in COS-7 cells and in HEK-293 cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Coffer, P.J.; Woodgett, J.R.
Molecular cloning and characterisation of a novel putative protein-serine kinase related to the cAMP-dependent and protein kinase C families
Eur. J. Biochem.
205
1217
1992
Homo sapiens (P31749), Bos taurus (Q01314)
Manually annotated by BRENDA team
Coffer, P.J.; Woodgett, J.R.
Molecular cloning and characterisation of a novel putative protein-serine kinase related to the cAMP-dependent and protein kinase C families
Eur. J. Biochem.
201
475-481
1991
Homo sapiens (P31749), Homo sapiens, Bos taurus (Q01314), Bos taurus
Manually annotated by BRENDA team
Rowles, J.; Slaughter, C.; Moomaw, C.; Hsu, J.; Cobb, M.H.
Purification of casein kinase I and isolation of cDNAs encoding multiple casein kinase I-like enzymes
Proc. Natl. Acad. Sci. USA
88
9548-9552
1991
Bos taurus (P35507), Bos taurus (P67827), Bos taurus
Manually annotated by BRENDA team
Litchfield, D.W.; Lozeman, F.J.; Piening, C.; Sommercorn, J.; Takio, K.; Walsh, K.A.; Krebs, E.G.
Subunit structure of casein kinase II from bovine testis. Demonstration that the alpha and alpha' subunits are distinct polypeptides
J. Biol. Chem.
265
7638-7644
1990
Bos taurus (P20427), Bos taurus, Homo sapiens (P68400)
Manually annotated by BRENDA team
Damuni, Z.; Reed, L.J.
Purification and properties of a protamine kinase and a type II casein kinase from bovine kidney mitochondria
Arch. Biochem. Biophys.
262
574-584
1988
Bos taurus
Manually annotated by BRENDA team
Damuni, Z.; Amick, G.D.; Sneed, T.R.
Purification and properties of a distinct protamine kinase from the cytosol of bovine kidney cortex
J. Biol. Chem.
264
6412-6416
1989
Bos taurus
Manually annotated by BRENDA team
Amick, G.D.; Reddy, S.A.G.; Damuni, Z.
Purification and properties of a protamine kinase from bovine kidney microsomes
Arch. Biochem. Biophys.
297
80-85
1992
Bos taurus
Manually annotated by BRENDA team
Amick, G.D.; Reddy, S.A.G.; Damuni, Z.
Protein phosphatase 2A is a specific protamine-kinase-inactivating phosphatase
Biochem. J.
287
1019-1022
1992
Bos taurus
-
Manually annotated by BRENDA team
Damuni, Z.
Inactivation of bovine kidney cytosolic protamine kinase by the catalytic subunit of protein phosphatase 2A
Biochem. Biophys. Res. Commun.
166
449-456
1990
Bos taurus
Manually annotated by BRENDA team
Amick, G.D.; Damuni, Z.
Protamine kinase phosphorylates eukaryotic protein synthesis initiation factor 4E
Biochem. Biophys. Res. Commun.
183
431-437
1992
Bos taurus
Manually annotated by BRENDA team
Mukai, H.
The structure and function of PKN, a protein kinase having a catalytic domain homologous to that of PKC
J. Biochem.
133
17-27
2003
Asteroidea, Bos taurus, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Xenopus laevis
Manually annotated by BRENDA team
Agarwal-Mawal, A.; Qureshi, H.Y.; Cafferty, P.W.; Yuan, Z.; Han, D.; Lin, R.; Paudel, H.K.
14-3-3 connects glycogen synthase kinase-3 beta to tau within a brain microtubule-associated tau phosphorylation complex
J. Biol. Chem.
278
12722-12728
2003
Bos taurus
Manually annotated by BRENDA team
Akita, S.; Umezawa, N.; Kato, N.; Higuchi, T.
Array-based fluorescence assay for serine/threonine kinases using specific chemical reaction
Bioorg. Med. Chem.
16
7788-7794
2008
Bos taurus, Homo sapiens (P31749)
Manually annotated by BRENDA team