Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.11.1 - non-specific serine/threonine protein kinase and Organism(s) Streptomyces coelicolor and UniProt Accession P54740

for references in articles please use BRENDA:EC2.7.11.1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Streptomyces coelicolor
UNIPROT: P54740
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Streptomyces coelicolor
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
hide
ATP
+
a [protein]-(L-serine/L-threonine)
=
ADP
+
a [protein]-(L-serine/L-threonine) phosphate
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
protein kinase a, 14-3-3, camkii, p-akt, lrrk2, c-jun n-terminal kinase, serine/threonine kinase, raf-1, protein kinase b, gsk-3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
serine/threonine protein kinase pkaB
-
A-kinase
-
-
-
-
A-T, mutated
-
-
-
-
A-T, mutated homolog
-
-
-
-
AMH type II receptor
-
-
-
-
Anti-sigma B factor rsbT
-
-
-
-
AP50 kinase
-
-
-
-
Ataxia telangiectasia mutated
-
-
-
-
Ataxia telangiectasia mutated homolog
-
-
-
-
ATP-protein transphosphorylase
-
-
-
-
betaIIPKC
-
-
-
-
Breast-tumor-amplified kinase
-
-
-
-
Calcium-dependent protein kinase C
-
-
-
-
Calcium/phospholipid-dependent protein kinase
-
-
-
-
cAMP-dependent protein kinase A
-
-
-
-
casein kinase
-
-
-
-
casein kinase 2
-
-
-
-
casein kinase I
-
-
-
-
casein kinase II
-
-
-
-
casein kinase-2
-
-
-
-
CDC25 suppressing protein kinase
-
-
-
-
Cell division cycle 2-like
-
-
-
-
CK-2
-
-
-
-
CK2
-
-
-
-
CK2-alpha
-
-
-
-
CKI
-
-
-
-
CKII
-
-
-
-
CLP-36 interacting kinase
-
-
-
-
cyclic monophosphate-dependent protein kinase
-
-
-
-
cyclic nucleotide-dependent protein kinase
-
-
-
-
cyclin-dependent kinase
-
-
-
-
cytidine 3',5'-cyclic monophosphate-responsive protein kinase
-
-
-
-
DNA-PKcs
-
-
-
-
DNPK1
-
-
-
-
dSTPK61
-
-
-
-
G11 protein
-
-
-
-
hARK1
-
-
-
-
histone kinase
-
-
-
-
hPDK1
-
-
-
-
Hpr kinase
-
-
-
-
hPSK
-
-
-
-
HSPK 21
-
-
-
-
HSPK 36
-
-
-
-
hydroxyalkyl-protein kinase
-
-
-
-
Kinase interacting with stathmin
-
-
-
-
kinase, casein (phosphorylating)
-
-
-
-
kinase, protamine (phosphorylating)
-
-
-
-
kinase, protein (phosphorylating)
-
-
-
-
kinase, protein, A (phosphorylating)
-
-
-
-
kinase, protein, C (phosphorylating)
-
-
-
-
Lymphocyte-oriented kinase
-
-
-
-
M phase-specific cdc2 kinase
-
-
-
-
mitogen-activated S6 kinase
-
-
-
-
mPDK1
-
-
-
-
MPSK
-
-
-
-
Myristoylated and palmitoylated serine-threonine kinase
-
-
-
-
NY-REN-55 antigen
-
-
-
-
P-CIP2
-
-
-
-
P460
-
-
-
-
p46Eg265
-
-
-
-
p46XlEg22
-
-
-
-
p82 kinase
-
-
-
-
pEg2
-
-
-
-
phosphorylase b kinase kinase
-
-
-
-
Phototropin
-
-
-
-
Pim-2h
-
-
-
-
PKA
-
-
-
-
PkB kinase
-
-
-
-
pp39-mos
-
-
-
-
Pre-mRNA protein kinase
-
-
-
-
protamine kinase
-
-
-
-
protein glutamyl kinase
-
-
-
-
protein kinase (phosphorylating)
-
-
-
-
Protein kinase B kinase
-
-
-
-
protein kinase CK2
-
-
-
-
Protein kinase Krct
-
-
-
-
Protein kinase MST
-
-
-
-
protein kinase p58
-
-
-
-
Protein kinase PKL12
-
-
-
-
protein phosphokinase
-
-
-
-
protein serine kinase
-
-
-
-
protein serine-threonine kinase
-
-
-
-
protein-aspartyl kinase
-
-
-
-
protein-cysteine kinase
-
-
-
-
protein-serine kinase
-
-
-
-
protein-serine/threonine kinase
-
-
-
-
PRP4 kinase
-
-
-
-
PRP4 pre-mRNA processing factor 4 homolog
-
-
-
-
PSK-H1
-
-
-
-
Raf kinase
-
-
-
-
Raf-1
-
-
-
-
ratAurA
-
-
-
-
ribosomal protein S6 kinase II
-
-
-
-
ribosomal S6 protein kinase
-
-
-
-
RP1 protein
-
-
-
-
S-receptor kinase
-
-
-
-
serine kinase
-
-
-
-
serine protein kinase
-
-
-
-
serine(threonine) protein kinase
-
-
-
-
serine-specific protein kinase
-
-
-
-
serine-threonine kinase
-
serine/threonine kinase
Serine/threonine kinase 15
-
-
-
-
Serine/threonine kinase Ayk1
-
-
-
-
serine/threonine protein kinase
serine/threonine protein kinase afsK
-
serine/threonine protein kinase pkaA
-
Serine/threonine-protein kinase NRK2
-
-
-
-
Serine/threonine-protein kinase NYD-SPK
-
-
-
-
SRK
-
-
-
-
Ste20-like kinase
-
-
-
-
STE20-like kinase MST1
-
-
-
-
STE20-like kinase MST2
-
-
-
-
STE20-like kinase MST3
-
-
-
-
Switch protein/serine kinase
-
-
-
-
T-antigen kinase
-
-
-
-
threonine-specific protein kinase
-
-
-
-
Twitchin kinase
-
-
-
-
type-2 casein kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (non-specific)
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
CAS REGISTRY NUMBER
COMMENTARY hide
191808-15-8
phosphoinositide dependent protein kinase 1
340830-03-7
-
37278-10-7
-
377752-08-4
ribosomal protein S6 kinase 2
389133-24-8
ribosomal S6 kinase 3
52660-18-1
casein kinase, protein kinase CK2
9026-43-1
this CAS Reg. No. encompasses a great variety of protein kinases including the serine/threonine specific kinases
90698-26-3
ribosomal protein S6 kinase 1
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + AfsR
ADP + ?
show the reaction diagram
phosphorylation at both Ser and Thr residues
-
-
?
ATP + AfsR protein
ADP + AfsR phosphoprotein
show the reaction diagram
ATP + KbpA
ADP + phospho-KbpA
show the reaction diagram
recombinant His-tagged substrate, 28 kDa
-
-
?
ATP + KbpA protein
ADP + phosphorylated KbpA protein
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + AfsR protein
ADP + AfsR phosphoprotein
show the reaction diagram
-
kinase AfsK specifically phosphorylates AfsR, a transcriptional activator with ATPase activity, starting a signal transduction pathway via induction of AfsS expression, thereby controlling the secondary metabolism of the bacterium, overview
-
-
?
ATP + KbpA protein
ADP + phosphorylated KbpA protein
show the reaction diagram
-
-
-
?
additional information
?
-
-
AfsK is a Hanks-type protein kinase
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
staurosporine
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KbpA
-
an AfsK-binding protein activates and modulates the enzyme activity
-
additional information
recombinant PkaE is activated by autophosphorylation at threonine residues only, but not serine or tyrosine residues
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
gene pkaE is expressed during the late growth phase in Streptomyces coelicolor A3 (2) M145, which corresponds to the production time of blue pigments, pkaE is expressed during the late growth phase (60 to 84 h), but not the early growth phase (36 to 48 h)
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the N-terminal region of PkaE is similar to the catalytic domains of several bacterial serine/ threonine protein kinases. In contrast, the C-terminal region of PkaE has no significant sequence similarity to any proteins
malfunction
disruption of chromosomal pkaE results in the overproduction of the actinorhodin-related blue pigment antibiotics
physiological function
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
63900
deduced from cDNA
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
phosphorylated mainly at Thr
phosphoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K42Q
site-directed mutagenesis, inactive mutant
K42R
in vitro phosphorylation assay shows that mutant protein is not able to phosphorylate itself
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione Sepharose column chromatography, and gel filtration
recombinant GST-tagged wild-type enzyme and mutant enzymes, and His-tagged enzyme mutant K42N from Escherichia coli strain BL21(DE3)plysS by glutathione or nickel affinity chromatography, and gel filtration
using Ni-NTA chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression of AsfK and AsfR in Escherichia coli, expression of PkaG and SCD10.09 in Escherichia coli
-
gene pkaE, recombinant expression of GST-tagged wild-type enzyme and mutant enzymes, and of His-tagged enzyme mutant K42N in Escherichia coli strain BL21(DE3)plysS
overexpression in Escherichia coli
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
the recombinant enzyme is refolded into active forms by stepwise dialysis against buffer containing 50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 1 mM EDTA, and 1 mM dithiothreitol with decreasing urea concentration of 4, 2, and 1 M at 4 °C for 1 h
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bentley, S.D.; Chater, K.F.; Cerdeno-Tarraga, A.M.; et al.
Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)
Nature
417
141-147
2002
Streptomyces coelicolor (P54739), Streptomyces coelicolor (P54740), Streptomyces coelicolor (P54741)
Manually annotated by BRENDA team
Urabe, H.; Ogawara, H.
Cloning, sequencing and expression of serine/threonine kinase-encoding genes from Streptomyces coelicolor A3(2)
Gene
153
99-104
1995
Streptomyces coelicolor (P54739), Streptomyces coelicolor (P54740)
Manually annotated by BRENDA team
Matsumoto, A.; Hong, S.K.; Ishizuka, H.; Horinouchi, S.; Beppu, T.
Phosphorylation of the AfsR protein involved in secondary metabolism in Streptomyces species by a eukaryotic-type protein kinase
Gene
146
47-56
1994
Streptomyces coelicolor (P54741)
Manually annotated by BRENDA team
Ueda, K.; Umeyama, T.; Beppu, T.; Horinouchi, S.
The aerial mycelium-defective phenotype of Streptomyces griseus resulting from A-factor deficiency is suppressed by a Ser/Thr kinase of S. coelicolor A3(2)
Gene
169
91-95
1996
Streptomyces coelicolor (P54741), Streptomyces coelicolor
Manually annotated by BRENDA team
Horinouchi, S.
AfsR as an integrator of signals that are sensed by multiple serine/threonine kinases in Streptomyces coelicolor A3(2)
J. Ind. Microbiol. Biotechnol.
30
462-467
2003
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Manually annotated by BRENDA team
Urabe, H.; Aoyagi, N.; Ogawara, H.; Motojima, K.
Expression and characterization of the Streptomyces coelicolor serine/threonine protein kinase PkaD
Biosci. Biotechnol. Biochem.
72
778-785
2008
Streptomyces coelicolor (O83032), Streptomyces coelicolor
Manually annotated by BRENDA team
Urabe, H.; Ogawara, H.; Motojima, K.
Expression and characterization of Streptomyces coelicolor serine/threonine protein kinase PkaE
Biosci. Biotechnol. Biochem.
79
855-862
2015
Streptomyces coelicolor (Q9ZNB6), Streptomyces coelicolor, Streptomyces coelicolor A3 (2) M145 (Q9ZNB6)
Manually annotated by BRENDA team
Urabe, H.; Ogawara, H.; Motojima, K.
Expression and characterization of Streptomyces coelicolor serine/threonine protein kinase PkaE
Biosci. Biotechnol. Biochem.
79
855-862
2015
Streptomyces coelicolor (Q9ZNB6), Streptomyces coelicolor A3(2) M145 (Q9ZNB6)
-
Manually annotated by BRENDA team