Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.1.86 - NADH kinase and Organism(s) Arabidopsis thaliana and UniProt Accession Q56YN3

for references in articles please use BRENDA:EC2.7.1.86
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
CTP, ITP, UTP and GTP can also act as phosphate donors (in decreasing order of activity). The enzyme is specific for NADH. Activated by acetate.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q56YN3
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
nadh kinase, pos5p, atp-nadh kinase, atp:nadh 2'-phosphotransferase, nicotinamide adenine dinucleotide hydride kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP:NADH 2'-phosphotransferase
-
-
-
-
ATP:NADH2 2'-phosphotransferase
-
-
-
-
diphosphopyridine nucleotide (reduced) kinase
-
-
DPNH kinase
-
-
-
-
kinase, reduced nicotinamide adenine dinucleotide (phosphorylating)
-
-
-
-
NAD(H) kinase
-
-
NADH kinase
-
-
-
-
NADH2 kinase
-
-
-
-
NADK1
-
isoform
nicotinamide adenine dinucleotide (reduced) kinase
-
-
reduced diphosphopyridine nucleotide kinase
-
-
-
-
reduced nicotinamide adenine dinucleotide kinase (phosphorylating)
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:NADH 2'-phosphotransferase
CTP, ITP, UTP and GTP can also act as phosphate donors (in decreasing order of activity). The enzyme is specific for NADH. Activated by acetate.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-39-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + NAD+
ADP + NADP+
show the reaction diagram
-
-
-
?
ATP + NADH
ADP + NADPH
show the reaction diagram
shows a 2fold preference for NADH over NAD+
-
-
?
ATP + NAD+
ADP + NADP+
show the reaction diagram
-
NADH is preferred 100fold over NAD+
-
-
?
ATP + NADH
ADP + NADPH
show the reaction diagram
CTP + NADH
CDP + NADPH
show the reaction diagram
-
ATP is preferred substrate
-
-
?
GTP + NADH
GDP + NADPH
show the reaction diagram
-
ATP is preferred substrate
-
-
?
UTP + NADH
UDP + NADPH
show the reaction diagram
-
ATP is preferred substrate
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required for activity
additional information
no significant increase in NAD(H) kinase activity is detected in the presence of Ca2+ /calmodulin
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diphosphate
-
mixed inhibitor both to NADH and ATP
p-chloromercuribenzoate
-
0.01 mM, 42% residual activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.062
ATP
0.759
CTP
-
pH 7.9, 25°C, cosubstrate NADH
0.461
GTP
-
pH 7.9, 25°C, cosubstrate NADH
2.39
NAD+
0.042
NADH
0.156
UTP
-
pH 7.9, 25°C, cosubstrate NADH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.4
in the presence of 0.5 mM EGTA, using NAD+ as a substrate
5.75
in the presence of 0.5 mM EGTA, using NADH as a substrate
15.1
-
NAD+ as substrate, pH 7.9, 25°C
15.6
-
pH 7.9, 25°C, substrate NAD+
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Ws-4
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
restricted to lateral root primordium
Manually annotated by BRENDA team
-
restricted primarily to leaf vasculature
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
NADK3 localizes to the peroxisomal matrix via a novel type 1 peroxisomal targeting signal
Manually annotated by BRENDA team
additional information
-
not: mitochondrial
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NADK1_ARATH
524
0
58245
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58250
calculated from amino acid sequence
35000
-
2 * 35000, SDS-PAGE
58000
-
isoform NADK1
78000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 35000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HiTrap Chelating HP column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
a NADK3-GFP reporter fusion construct is expressed in Arabidopsis suspension-cultured cells
-
expression in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
NADK3 activity is first observed in stage 1.0-1.02, most notably in the procambium and vasculature of cotyledons
-
transcription of isoform NADK1 is upregulated by 3 and 8fold after treatment of plant cells with 5 mM H2O2 and irradiation, respectively
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Turner, W.L.; Waller, J.C.; Snedden, W.A.
Identification, molecular cloning and functional characterization of a novel NADH kinase from Arabidopsis thaliana (thale cress)
Biochem. J.
385
217-223
2005
Arabidopsis thaliana
Manually annotated by BRENDA team
Berrin, J.; Brutesco, C.; Montillet, J.; Kazmaier, M.; Pierrugues, O.; Alonso, B.; Roby, D.
Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana
Mol. Genet. Genomics
273
10-19
2005
Arabidopsis thaliana (Q56YN3)
Manually annotated by BRENDA team
Chai, M.F.; Wei, P.C.; Chen, Q.J.; An, R.; Chen, J.; Yang, S.; Wang, X.C.
NADK3, a novel cytoplasmic source of NADPH, is required under conditions of oxidative stress and modulates abscisic acid responses in Arabidopsis
Plant J.
47
665-674
2006
Arabidopsis thaliana
Manually annotated by BRENDA team
Kawai, S.; Murata, K.
Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H)
Biosci. Biotechnol. Biochem.
72
919-930
2008
Arabidopsis thaliana, Saccharomyces cerevisiae (C7GJD6)
Manually annotated by BRENDA team
Waller, J.C.; Dhanoa, P.K.; Schumann, U.; Mullen, R.T.; Snedden, W.A.
Subcellular and tissue localization of NAD kinases from Arabidopsis: compartmentalization of de novo NADP biosynthesis
Planta
231
305-317
2010
Arabidopsis thaliana
Manually annotated by BRENDA team