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Information on EC 2.7.1.82 - ethanolamine kinase and Organism(s) Homo sapiens and UniProt Accession Q9HBU6
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2.7.1.82 ethanolamine kinaseSpecify your search results
Word Map
- 2.7.1.82
- choline
- phosphatidylethanolamine
- phosphoethanolamine
- cdp-ethanolamine
- phosphocholine
-
kennedy
-
ptdetn
- cdp-choline
-
setbp1
- medicine
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
ethanolamine kinase, etnk1, etnk2, etn kinase, ethanolamine kinase 1, ethanolamine kinase 2, treki1, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
EKI
-
-
-
-
EKI1
-
-
-
-
ethanolamine phosphokinase
-
-
-
-
EtnK
-
-
-
-
kinase, ethanolamine (phosphorylating)
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:ethanolamine O-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY
9075-78-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
rate-controlling step in phosphatidylethanolamine biosynthesis
-
-
?
additional information
?
-
-
rate-controlling step in phosphatidylethanolamine biosynthesis
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
rate-controlling step in phosphatidylethanolamine biosynthesis
-
-
?
additional information
?
-
-
rate-controlling step in phosphatidylethanolamine biosynthesis
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
expression levels of splicing variant ek2alpha is repressed in the HepG2 cells
additional information
additional information
expression profile of the enzyme in cancer cell lines, overview
additional information
expression profile of the enzyme in cancer cell lines, overview
additional information
expression profile of the enzyme in cancer cell lines, overview. Splice variant ek2beta exhibits a markedly low mRNA transcript level in all three of the cancer cell lines
additional information
expression profile of the enzyme in cancer cell lines, overview. Splice variant ek2beta exhibits a markedly low mRNA transcript level in all three of the cancer cell lines
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
human ethanolamine kinase exists as three isoforms: EK1, EK2alpha and EK2beta, encoded by two separate genes; ek1, which produces the ek1 transcript variant 1, and ek2, which undergoes alternative splicing to produce ek2alpha (NCBI reference: NM_018208.2) and ek2beta (GenBank reference: AK001623.1) transcript variants. ek2alpha and ek2beta are respectively translated into EK2alpha and EK2beta
malfunction
somatic enzyme mutations targeted to two contiguous amino acids in the ETNK1 kinase domain are involved in systemic mastocytosis with eosinophilia and chronic myelomonocytic leukemia, phenotypes, overview
metabolism
evolution
human ethanolamine kinase exists as three isoforms: EK1, EK2alpha and EK2beta, encoded by two separate genes; ek1, which produces the ek1 transcript variant 1, and ek2, which undergoes alternative splicing to produce ek2alpha (NCBI reference: NM_018208.2) and ek2beta (GenBank reference: AK001623.1) transcript variants. ek2alpha and ek2beta are respectively translated into EK2alpha and EK2beta
metabolism
choline kinase and ethanolamine kinase are enzymes that initiate the first step in the Kennedy pathway, resulting in the biosynthesis of phosphatidylcholine and phosphatidylethanolamine. Expression profiling of ethanolamine kinase in MCF-7, HCT-116 and Hep-G2 cells, and the transcriptional regulation by epigenetic modification
physiological function
splicing variant ek2beta exhibits a markedly low mRNA transcript level in all three of the cancer cell lines examined, thus suggesting that transcription of the ek2beta gene may be negligible and this isoform may not have a significant role in phosphatidyletanolamine synthesis in the three cancer cell lines
metabolism
choline kinase and ethanolamine kinase are enzymes that initiate the first step in the Kennedy pathway, resulting in the biosynthesis of phosphatidylcholine and phosphatidylethanolamine. Expression profiling of ethanolamine kinase in MCF-7, HCT-116 and Hep-G2 cells, and the transcriptional regulation by epigenetic modification
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). EKI1_HUMAN
452
1
50968
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D816V
naturally occuring mutation involved in adult systemic mastocytosis, chronic myelomonocytic leukemia, or eosinophilia
G245A
naturally occuring mutation involved in adult systemic mastocytosis,with chronic myelomonocytic leukemia and/or eosinophilia, inactive mutant
N244K
naturally occuring mutation involved in adult systemic mastocytosis, with chronic myelomonocytic leukemia and/or eosinophilia, inactive mutant
N244S
naturally occuring mutation involved in adult systemic mastocytosis, with chronic myelomonocytic leukemia and/or eosinophilia, inactive mutant
N244T
naturally occuring mutation involved in adult systemic mastocytosis, with chronic myelomonocytic leukemia and/or eosinophilia, inactive mutant
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene eki1, a Sp site at position (-40/-31) is essential for the basal transcription of this gene, quantitative real-time PCR transcription analysis of ek1 mRNA, Identification of important regulatory regions in the ek1 gene promoter by analysis of 4 promoter constructs, trichostatin A activates basal ek1 promoter activity via Sp(-40/-31) site
gene ETNK1, overexpression of wild-type and N244S mutant enzymes in murine Ba/F3 cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
treatment of HCT-116 cells with trichostatin A (TSA), a histone deacetylase inhibitor, significantly upregulates the eki1 promoter activity through the Sp(-40/-31) site and increases the endogenous expression of gene eki1. Trichostatin A increases the binding of Sp1, Sp3 and RNA polymerase II to the ek1 promoter in HCT116 cells, molecular mechanism, overview. The effect of TSA on ek1 promoter activity is cell-line specific as TSA treatment does not affect ek1 promoter activity in Hep-G2 cells
substantial induction of splicing variant ek2alpha mRNA expression in response to trichostatin A treatment in Hep-G2 cells, the elevated ek2alpha mRNA expression may be due to the accumulation of acetylated histones H3 and H4, located around the promoter regions. Trichostatin A may also induce the recruitment of euchromatic markers and RNA polymerase II to the transcription factor complex that binds to the promoter, thus facilitating gene transcription
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ishidate, K.
Choline/ethanolamine kinase from mammalian tissues
Biochim. Biophys. Acta
1348
70-78
1997
Saccharomyces cerevisiae, Homo sapiens, Rattus norvegicus
Draus, E.; Niefield, J.; Vietor, K.; Havsteen, B.
Isolation and characterization of the human liver ethanolamine kinase
Biochim. Biophys. Acta
1045
195-204
1990
Homo sapiens
Lykidis, A.; Wang, J.; Karim, M.A.; Jackowski, S.
Overexpression of a mammalian ethanolamine-specific kinase accelerates the CDP-ethanolamine pathway
J. Biol. Chem.
276
2174-2179
2001
Homo sapiens (Q9HBU6), Homo sapiens
Lasho, T.L.; Finke, C.M.; Zblewski, D.; Patnaik, M.; Ketterling, R.P.; Chen, D.; Hanson, C.A.; Tefferi, A.; Pardanani, A.
Novel recurrent mutations in ethanolamine kinase 1 (ETNK1) gene in systemic mastocytosis with eosinophilia and chronic myelomonocytic leukemia
Blood Cancer J.
5
e275
2015
Homo sapiens (Q9HBU6), Homo sapiens
Ling, C.S.; Yin, K.B.; Cun, S.T.; Ling, F.L.
Expression profiling of choline and ethanolamine kinases in MCF7, HCT116 and HepG2 cells, and the transcriptional regulation by epigenetic modification
Mol. Med. Rep.
11
611-618
2015
Homo sapiens (Q9HBU6), Homo sapiens (Q9NVF9)
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