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Information on EC 2.7.1.82 - ethanolamine kinase and Organism(s) Mus musculus and UniProt Accession O54804

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This record set is specific for:
Mus musculus
UNIPROT: O54804 not found.
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
ethanolamine kinase, etnk1, etnk2, etn kinase, ethanolamine kinase 1, ethanolamine kinase 2, treki1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
EKI
-
-
-
-
EKI1
-
-
-
-
ethanolamine kinase 2
-
ethanolamine phosphokinase
-
-
-
-
EtnK
-
-
-
-
kinase, ethanolamine (phosphorylating)
-
-
-
-
additional information
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + ethanolamine = ADP + O-phosphoethanolamine
show the reaction diagram
choline kinase and ethanolamine kinase are 2 distinct enzymes
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:ethanolamine O-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9075-78-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + ethanolamine
ADP + O-phosphoethanolamine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
neither Ca2+ nor Mn2+ can substitute for Mg2+
Mg2+
-
Mg2+ required
Mn2+
-
inhibition
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,3-Dimercaptopropanol
-
-
Acetyl-beta-methylcholine chloride
-
-
Acetylcholine
-
-
betaine
-
not
Butyrylcholine iodide
-
-
butyrylthiocholine iodide
-
-
Ca2+
-
-
choline
-
-
cysteine
-
-
Methane sulfonylcholine chloride
-
-
Mn2+
-
-
p-chloromercuribenzoate
-
not
phosphorylcholine
-
-
Phosphorylethanolamine
-
-
Propionylcholine iodide
-
-
Succinylcholine chloride
-
-
additional information
-
not inhibitory: sodium arsenite
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 10.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
high expression of isoform Eki2 at the time of sex-differentiation
Manually annotated by BRENDA team
additional information
-
beta isozyme, almost equal distribution in all tissues examined
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
supernatant fraction
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHKA_MOUSE
453
0
51985
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
x * 47000, or x-y * 47000 + y * 43000, or x * 43000, active form consitsts of oligomers of alpha/beta isoforms, with relatively small parts of alpha/alpha or beta/beta homo-oligomers, SDS-PAGE
47000
-
x * 47000, or x-y * 47000 + y * 43000, or x * 43000, active form consitsts of oligomers of alpha/beta isoforms, with relatively small parts of alpha/alpha or beta/beta homo-oligomers, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oligomer
-
x * 47000, or x-y * 47000 + y * 43000, or x * 43000, active form consitsts of oligomers of alpha/beta isoforms, with relatively small parts of alpha/alpha or beta/beta homo-oligomers, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
targeted disruption of Eki2 gene. No abnormalities in deficient mice with regard to embryonic and adult testis morphology, differentiation, function or fertility, and no significant differences in litter size, pup mortality rates or distribution of the sexes among the offspring compared with wild-type
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or 0-4°C, crude cell extract, appreciable loss of activity
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in 293T cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Upreti, R.K.; Sanwal, G.G.; Krishnan, P.S.
Likely individuality of the enzymes catalyzing the phosphorylation of choline and ethanolamine
Arch. Biochem. Biophys.
174
658-665
1976
Duttaphrynus melanostictus, Capra hircus, Cavia porcellus, Gallus gallus, Columba livia, Hemidactylus sp., Heteropneustes fossilis, Mus musculus, Hoplobatrachus tigerinus, Rattus norvegicus
Manually annotated by BRENDA team
Aoyama, C.; Ohtani, A.; Ishidate, K.
Expression and characterization of the active molecular forms of choline/ethanolamine kinase-alpha and -beta in mouse tissues, including carbon tetrachloride-induced liver
Biochem. J.
363
777-784
2002
Mus musculus
Manually annotated by BRENDA team
Aoyama, C.; Yamazaki, N.; Terada, H.; Ishidate, K.
Structure and characterization of the genes for murine choline/ethanolamine kinase isozymes alpha and beta
J. Lipid Res.
41
452-464
2000
Mus musculus (O54804), Mus musculus (Q54AG5), Mus musculus (Q9D4V0)
Manually annotated by BRENDA team
Sung, C.P.; Johnstone, R.M.
Phosphorylation of choline and ethanolamine in Ehrlich ascites-carcinoma cells
Biochem. J.
105
497-503
1967
Mus musculus
Manually annotated by BRENDA team
Tian, Y.; Jackson, P.; Gunter, C.; Wang, J.; Rock, C.O.; Jackowski, S.
Placental thrombosis and spontaneous fetal death in mice deficient in ethanolamine kinase 2
J. Biol. Chem.
281
28438-28449
2006
Mus musculus (A7MCT6)
Manually annotated by BRENDA team
Gustin, S.E.; Western, P.S.; McClive, P.J.; Harley, V.R.; Koopman, P.A.; Sinclair, A.H.
Testis development, fertility, and survival in ethanolamine kinase 2-deficient mice
Endocrinology
149
6176-6186
2008
Mus musculus
Manually annotated by BRENDA team