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Information on EC 2.7.1.76 - 2'-deoxyadenosine kinase and Organism(s) Lactobacillus acidophilus and UniProt Accession P0C1F9

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EC Tree
IUBMB Comments
2'-Deoxyguanosine can also act as acceptor. Possibly identical with EC 2.7.1.74 deoxycytidine kinase.
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This record set is specific for:
Lactobacillus acidophilus
UNIPROT: P0C1F9
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Word Map
The taxonomic range for the selected organisms is: Lactobacillus acidophilus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
deoxyadenosine kinase, 2'-deoxyadenosine kinase, dado/dcyd kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
deoxycytidine kinase/deoxyadenosine kinase, dCK/dAK
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deoxyguanosine kinase/deoxyadenosine kinase, dGK/dAK
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dCyd kinase/dAdo kinase I
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dGuo kinase/dAdo kinase II
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kinase, deoxyadenosine (phosphorylating)
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-
-
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purine-deoxyribonucleoside kinase
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-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 2'-deoxyadenosine = ADP + dAMP
show the reaction diagram
random sequential substrate addition
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:2'-deoxyadenosine 5'-phosphotransferase
2'-Deoxyguanosine can also act as acceptor. Possibly identical with EC 2.7.1.74 deoxycytidine kinase.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-12-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2'-deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
-
?
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
-
-
?
ATP + deoxyadenosine
ADP + dAMP
show the reaction diagram
ATP + deoxycytidine
ADP + dCMP
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + deoxyadenosine
ADP + 5'-dAMP
show the reaction diagram
dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required for activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenine arabinoside
-
-
dATP
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kinetic, mechanism, protects corresponding active site against trypsin inactivation
dCTP
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kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP
dGTP
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kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP
Trypsin
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substrates protect, end-products, i.e. dATP, dGTP or dCTP, protect corresponding active sites
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
deoxyguanosine
0.1 mM, 5fold activation of recombinant dAK
2'-O-Cyclocytidine
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activation
5-Fluoro-2'-deoxycytidine
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0.4 mM, 275% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.028 mM
cytosine arabinoside
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activation
deoxycytidine
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30 mM, 584% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.1 mM
deoxycytidine monophosphate
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10 mM, 409% activation of deoxyadenosine phosphorylation, half-maximal activation at 1.6 mM
deoxyguanosine
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5 mM, 330% activation of deoxyadenosine phosphorylation, half-maximal activation at 2.2 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.11 - 0.44
ATP
0.0028 - 0.0075
deoxyadenosine
additional information
additional information
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kinetic study
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001 - 0.0014
dATP
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27200
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1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
29000
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1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D78A
mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity
D78E
mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity
D78N
mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity
D84A
mutation in dGK of dAK/dGK, increase of dAK activity
D84E
mutation in dGK of dAK/dGK, increase of dAK activity
D84N
mutation in dGK of dAK/dGK, increase of dAK activity
R79k
2 types of mutants, one bears the mutation on both the dAK and dGK subunits while the other, called R79K:dGK, has the mutation on the dGK subunit only, strong increase in dAK activity, activation of dAK by deoxyguanosine is nearly eliminated in the case of R79K:dGK, while for the tandem mutated R79K mutant a 30% inhibition is observed, inhibition by dATP is reduced
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dATP stabilizes
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
dATP stabilizes during storage
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant dGK/dAK, dATP-Sepharose affinity chromatography
affinity chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of dGK/dAK in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hong, Y.S.; Ma, G.T.; Ives, D.H.
Directed mutagenesis of deoxyguanosine site at arginine 79 up-regulates turnover on deoxyadenosine kinase subunit of heterodimeric enzyme from Lactobacillus acidophilus R26
J. Biol. Chem.
270
6602-6606
1995
Lactobacillus acidophilus (P0C1F9), Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396 (P0C1F9)
Manually annotated by BRENDA team
Ikeda, S.; Ma, G.T.; Ives, D.H.
Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitors
Biochemistry
33
5328-5334
1994
Lactobacillus acidophilus, Lactobacillus acidophilus ATCC 700396
Manually annotated by BRENDA team
Ma, G.T.; Hong, Y.S.; Ives, D.H.
Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26
J. Biol. Chem.
270
6595-6601
1995
Lactobacillus acidophilus (P0C1F9), Lactobacillus acidophilus ATCC 700396 (P0C1F9)
Manually annotated by BRENDA team
Deibler, R.D.; Reznik, R.B.; Ives, D.H.
Deoxynucleoside kinases from Lactobacilli. Separate interacting sites for deoxycytidine and deoxyadenosine
J. Biol. Chem.
252
8240-8244
1977
Lactobacillus acidophilus
Manually annotated by BRENDA team
Ikeda, S.; Swenson, R.P.; Ives, D.H.
Amino-terminal nucleotide-binding sequences of a Lactobacillus deoxynucleoside kinase complex isolated by novel affinity chromatography
Biochemistry
27
8648-8652
1988
Lactobacillus acidophilus
Manually annotated by BRENDA team
Park, I.; Kim, E.A.; Bang, K.S.; Kim, S.H.; Kim, G.N.; Lee, M.K.; Kil, J.O.
Protein engineering of deoxynucleoside kinase from Lactobacillus acidophilus: effect of site-directed mutagenesis on microbial growth
J. Food Sci. Nutr.
6
79-81
2001
Lactobacillus acidophilus (P0C1F9), Lactobacillus acidophilus ATCC 700396 (P0C1F9)
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Manually annotated by BRENDA team