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Information on EC 2.7.1.45 - 2-dehydro-3-deoxygluconokinase and Organism(s) Sulfurisphaera tokodaii and UniProt Accession Q96XN9

for references in articles please use BRENDA:EC2.7.1.45
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EC Tree
IUBMB Comments
The enzyme shows no activity with 2-dehydro-3-deoxy-D-galactonate . cf. EC 2.7.1.178, 2-dehydro-3-deoxyglucono/2-dehydro-3-deoxygalactonokinase.
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This record set is specific for:
Sulfurisphaera tokodaii
UNIPROT: Q96XN9
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The taxonomic range for the selected organisms is: Sulfurisphaera tokodaii
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
2-keto-3-deoxygluconate kinase, 2-ketogluconate kinase, kdgk-1, kdg kinase, 2-keto-3-deoxy-d-gluconate kinase, hvo_0549, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-keto-3-deoxy-D-gluconic acid kinase
-
-
-
-
2-keto-3-deoxygluconate kinase
2-keto-3-deoxygluconokinase
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-
-
-
ketodeoxygluconokinase
-
-
-
-
kinase, 2-keto-3-deoxyglucono- (phosphorylating)
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:2-dehydro-3-deoxy-D-gluconate 6-phosphotransferase
The enzyme shows no activity with 2-dehydro-3-deoxy-D-galactonate [1]. cf. EC 2.7.1.178, 2-dehydro-3-deoxyglucono/2-dehydro-3-deoxygalactonokinase.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-54-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
show the reaction diagram
-
-
-
?
ATP + 2-keto-D-gluconate
ADP + 6-phospho-2-keto-D-gluconate
show the reaction diagram
-
-
-
?
GTP + 2-dehydro-3-deoxy-D-gluconate
GDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
show the reaction diagram
94% of the activity with ATP
-
-
?
ITP + 2-dehydro-3-deoxy-D-gluconate
IDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
show the reaction diagram
66% of the activity with ATP
-
-
?
TTP + 2-dehydro-3-deoxy-D-gluconate
TDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
show the reaction diagram
43% of the activity with ATP
-
-
?
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
show the reaction diagram
-
-
-
?
ATP + 2-keto-D-gluconate
ADP + 6-phospho-2-keto-D-gluconate
show the reaction diagram
-
-
-
?
GTP + 2-dehydro-3-deoxy-D-gluconate
GDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
show the reaction diagram
94% activity compared to the substrate combination with ATP
-
-
?
ITP + 2-dehydro-3-deoxy-D-gluconate
IDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
show the reaction diagram
66% activity compared to the substrate combination with ATP
-
-
?
TTP + 2-dehydro-3-deoxy-D-gluconate
TDP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
show the reaction diagram
43% activity compared to the substrate combination with ATP
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
98% of the activity with Mg2+
Mg2+
most effective
Mn2+
73% of the activity with Mg2+
Ni2+
92% of the activity with Mg2+
Zn2+
75% of the activity with Mg2+
Co2+
10 mM, required for activity, 98% relative activity compared to Mg2+
Mg2+
10 mM, required for activity, most effective cation
Mn2+
10 mM, required for activity, 73% relative activity compared to Mg2+
Ni2+
10 mM, required for activity, 92% relative activity compared to Mg2+
Zn2+
10 mM, required for activity, 75% relative activity compared to Mg2+
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.027
2-dehydro-3-deoxy-D-gluconate
pH 8.5, 50°C
1.3
2-keto-D-gluconate
pH 8.5, 50°C
0.057
ATP
pH 8.5, 50°C
0.027
2-dehydro-3-deoxy-D-gluconate
in 50 mM Tris/HCl (pH 8.5) at 50°C
1.3
2-keto-D-gluconate
in 50 mM Tris/HCl (pH 8.5) at 50°C
0.057 - 0.11
ATP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.26
2-dehydro-3-deoxy-D-gluconate
pH 8.5, 50°C
26.3
2-keto-D-gluconate
pH 8.5, 50°C
8.26
2-dehydro-3-deoxy-D-gluconate
in 50 mM Tris/HCl (pH 8.5) at 50°C
26.3
2-keto-D-gluconate
in 50 mM Tris/HCl (pH 8.5) at 50°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
half of the maximum activity is obtained at pHs 6.5 and 9.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
65% of maximum activity
90
25% of maximum activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
gel filtration
34480
4 * 34480, calculated, 4 * 35000, SDS-PAGE
35000
4 * 34480, calculated, 4 * 35000, SDS-PAGE
120000
gel filtration
30000
4 * 30000, gel filtration
34480
calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
4 * 34480, calculated, 4 * 35000, SDS-PAGE
homotetramer
4 * 30000, gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
no activity
676988
4.5
no activity
676988
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
1 h, no loss of activity
85
1 h, 50% residual activity
90
10 min, 10% residual activity
50
the activity at 50°C is about 65% of the maximum
80
the purified recombinant enzyme is extremely thermostable and retains full activity after heating at 80°C for 1 h
90
the activity at 90°C is only about 25% of that at 80°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
Butyl Toyopearl column chromatography and Superdex 200 column gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expressed in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ohshima, T.; Kawakami, R.; Kanai, Y.; Goda, S.; Sakuraba, H.
Gene expression and characterization of 2-keto-3-deoxygluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of the aerobic and acidophilic hyperthermophile Sulfolobus tokodaii
Protein Expr. Purif.
54
73-78
2007
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii (Q96XN9)
Manually annotated by BRENDA team