Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.1.40 - pyruvate kinase and Organism(s) Schizosaccharomyces pombe and UniProt Accession Q10208

for references in articles please use BRENDA:EC2.7.1.40
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
UTP, GTP, CTP, ITP and dATP can also act as donors. Also phosphorylates hydroxylamine and fluoride in the presence of CO2.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: Q10208
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
pyruvate kinase, m2-pk, pyruvate kinase m2, l-pk, pyk, tum2-pk, pyruvate kinase type m2, liver pyruvate kinase, pyruvate kinase m2 isoform, m2-pyruvate kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CTHBP
-
-
-
-
cytosolic thyroid hormone binding protein
-
-
-
-
fluorokinase
-
-
-
-
kinase, fluoro- (phosphorylating)
-
-
-
-
kinase, pyruvate (phosphorylating)
-
-
-
-
L-PK
-
-
-
-
phosphoenol transphosphorylase
-
-
-
-
phosphoenolpyruvate kinase
-
-
-
-
pyruvate kinase muscle isozyme
-
-
-
-
pyruvate phosphotransferase
-
-
-
-
pyruvic kinase
-
-
-
-
R-type/L-type pyruvate kinase
-
-
-
-
red cell/liver pyruvate kinase
-
-
-
-
THBP1
-
-
-
-
VEG17
-
-
-
-
vegetative protein 17
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + pyruvate = ADP + phosphoenolpyruvate
show the reaction diagram
sigmoidal kinetics with respect to phosphoenolpyruvate
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:pyruvate 2-O-phosphotransferase
UTP, GTP, CTP, ITP and dATP can also act as donors. Also phosphorylates hydroxylamine and fluoride in the presence of CO2.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-59-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + phosphoenolpyruvate
ATP + pyruvate
show the reaction diagram
-
-
-
?
ADP + phosphoenolpyruvate
ATP + pyruvate
show the reaction diagram
-
-
-
-
ir
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-fructose 1,6-diphosphate
-
D-fructose 1,6-diphosphate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000 - 170000
analytical ultracentrifugation
55400
2 * 55400, SDS-PAGE, predominantly a dimer, but also some tetramer
100000
-
gel filtration
additional information
-
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 55400, SDS-PAGE, predominantly a dimer, but also some tetramer
tetramer
2 * 55400, SDS-PAGE, predominantly a dimer, but also some tetramer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis against 10 mM sodium phosphate or Tris-HCl, pH 7.5, for 18 h at 4°C, inactivates
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, in 10 mM Tris-HCl or sodium phosphate buffer, pH 7.5, 50% v/v glycerol, 18 h
-
4°C, in 10 mM Tris-HCl or sodium phosphate buffer, pH 7.5, 50% v/v glycerol, 18 h
-
glycerol stabilizes during storage
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial, dye-ligand chromatography
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Duncan, D.; McGowan, J.; Price, N.C.
Properties of pyruvate kinase from Schizosaccharomyces pombe
Biochem. Soc. Trans.
17
560-561
1989
Schizosaccharomyces pombe
-
Manually annotated by BRENDA team
Nairn, J.; Duncan, D.; Gray, L.M.; Urquhart, G.; Binnie, M.; Byron, O.; Fothergill-Gilmore, L.A.; Price, N.C.
Purification and characterization of pyruvate kinase from Schizosaccharomyces pombe: evidence for an unusual quaternary structure
Protein Expr. Purif.
14
247-253
1998
Schizosaccharomyces pombe (Q10208), Schizosaccharomyces pombe
Manually annotated by BRENDA team