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Information on EC 2.7.1.4 - fructokinase and Organism(s) Solanum lycopersicum and UniProt Accession O04897
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2.7.1.4 fructokinaseSpecify your search results
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The taxonomic range for the selected organisms is: Solanum lycopersicum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
fk, fructokinase, mdfrk2, hore_18220, mdfrk1, putative fructokinase, vc-fk, atfrk6, atfrk7, d-fructokinase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D-fructokinase
-
-
-
-
D-fructose(D-mannose)kinase
-
-
-
-
FK
-
-
-
-
Frk2
Frk3
kinase, fructo- (phosphorylating)
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
-
-, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:D-fructose 6-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY
9030-51-7
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
-
-
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
-
sucrose to starch synthetic pathway
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + D-fructose
ADP + D-fructose 6-phosphate
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
fructose metabolism
-
-
?
ATP + D-fructose
ADP + D-fructose 6-phosphate
-
sucrose to starch synthetic pathway
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
D-fructose
SlFRK2 and SlFRK3, are substrate inhibited. FRK1 is not inhibited by fructose
additional information
not inhibitory: D-glucose, and Mg2+ up to 10 mM
-
Mg2+
-
inhibition of FK1 above 1 mM, only little inhibition of FDIII, no inhibition of FKII
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.66 - 6.17
-
six closely grouped peptides, two-dimensional isoelectric focusing/SDS-PAGE
6.07 - 6.55
-
4 components, FKII, two-dimensional isoelectric focusing/SDS-PAGE
6.42 - 6.55
-
2 components, FKI, two-dimensional isoelectric focusing /SDS-PAGE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
expressed in phloem companion cells of both internal and external phloem
expressed in phloem companion cells of both internal and external phloem
SlFRK3 is expressed mainly in the cambium and newly differentiating xylem cells
SlFRK3 is expressed mainly in the cambium and newly differentiating xylem cells
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
malfunction
combined suppression of SlFRK1 and SlFRK2 severely inhibits plant growth and an anatomical analysis reveals a reduction in secondary xylem area and distorted phloem fibers characterized by thin cell walls and reduced lignification
physiological function
malfunction
combined suppression of SlFRK1 and SlFRK2 severely inhibits plant growth and an anatomical analysis reveals a reduction in secondary xylem area and distorted phloem fibers characterized by thin cell walls and reduced lignification
malfunction
specific suppression of SlFRK1 reduces water transport in stems, but had no other anatomical or phenotypic effects
physiological function
cotton plants transformed with a tomato fructokinase gene (FRK1) under the control of the CMV 35S promoter show increased fibre and leaf FRK activity over nonexpressing nulls, but not improved fibre length and strength. Compared with the nulls, FRK1 plants yield 13-100% more seed-cotton mass per boll and more bolls per. The enhanced yield is related to a greater seed number per boll for FRK1 plants. Photosynthetic rates are not appreciably different among genotypes. However, more area per leaf and leaf number for FRK1 plants than for nulls enhances the capacity for C gain. Larger leaf areas for FRK1 plants are associated with larger stem diameters
physiological function
fructose phosphorylation in the vascular tissues is highly important for cell-wall metabolism. SlFRK2 and SlFRK3 are involved in vascular tissue development and affects the shape, size, and cell-wall width of xylem vessels and xylem fibers
physiological function
SlFRK1 is involved in vascular tissue development and hydraulic conductivity in tomato plants. SlFRK1 is important for normal phloem fiber development together with SlFRK2
physiological function
suppression of isoform FRK3 reduces the stem xylem area, stem and root water conductance, and whole-plant transpiration, with minor effects on plant development. Suppression of FRK3 accompanied by partial suppression of isoform FRK2 induces significant growth-inhibition effects, including the wilting of mature leaves. These growth effects are imposed primarily by the leaves, whose petioles have unlignified, thinwalled xylem fibers with collapsed parenchyma cells around the vessels
physiological function
fructose phosphorylation in the vascular tissues is highly important for cell-wall metabolism. SlFRK2 and SlFRK3 are involved in vascular tissue development and affects the shape, size, and cell-wall width of xylem vessels and xylem fibers
physiological function
SlFRK1 is involved in vascular tissue development and hydraulic conductivity in tomato plants. SlFRK1 is important for normal phloem fiber development together with SlFRK2
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). O04897_SOLLC
347
0
37311
TrEMBL
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kanayama, Y.; Granot, D.; Dai, N.; Petreikov, M.; Schaffer, A.; Powell, A.; Bennett, A.B.
Tomato fructokinases exhibit differential expression and substrate regulation
Plant Physiol.
117
85-90
1998
Solanum lycopersicum
Martinez-Barajas, E.; Krohn, B.M.; Stark, D.M.; Randall, D.D.
Purification and characterization of recombinant tomato fruit (Lycopersicon esculentum Mill.) fructokinase expressed in Escherichia coli
Protein Expr. Purif.
11
41-46
1997
Solanum lycopersicum
Martinez-Barajas, E.; Randall, D.D.
Purification and characterization of fructokinase from developing tomato (Lycopersicon esculentum) fruits
Planta
199
451-458
1996
Solanum lycopersicum
-
Odanaka, S.; Bennett, A.B.; Kanayama, Y.
Distinct physiological roles of fructokinase isozymes revealed by gene-specific suppression of Frk1 and Frk2 expression in tomato
Plant Physiol.
129
1119-1126
2002
Solanum lycopersicum, Solanum lycopersicum Mill.
Pego, J.V.; Smeekens, S.C.
Plant fructokinases: a sweet family get-together
Trends Plant Sci.
5
531-536
2000
Arabidopsis thaliana, Persea americana, Camellia japonica, Gleditsia triacanthos, Hordeum vulgare, Lilium longiflorum, Solanum lycopersicum, Solanum lycopersicum (O04897), Solanum lycopersicum (Q42896), Pisum sativum, Spinacia oleracea, Zea mays, Solanum tuberosum (P37829), Beta vulgaris (Q42645)
Petreikov, M.; Dai, N.; Granot, D.; Schaffer, A.A.
Characterization of native and yeast-expressed tomato fruit fructokinase enzymes
Phytochemistry
58
841-847
2001
Solanum lycopersicum, Solanum lycopersicum Mill.
Schaffer, A.A.; Petreikov, M.
Inhibition of fructokinase and sucrose synthase by cytosolic levels of fructose in young tomato fruit undergoing transient starch synthesis
Physiol. Plant.
101
800-806
1997
Solanum lycopersicum
-
German, M.A.; Asher, I.; Petreikov, M.; Dai, N.; Schaffer, A.A.; Granot, D.
Cloning, expression and characterization of LeFRK3, the fourth tomato (Lycopersicon esculentum Mill.) gene encoding fructokinase
Plant Sci.
166
285-291
2004
Solanum lycopersicum (Q6VWJ5)
Damari-Weissler, H.; Kandel-Kfir, M.; Gidoni, D.; Mett, A.; Belausov, E.; Granot, D.
Evidence for intracellular spatial separation of hexokinases and fructokinases in tomato plants
Planta
224
1495-1502
2006
Solanum lycopersicum
Mukherjee, T.; Ivanova, M.; Dagda, M.; Kanayama, Y.; Granot, D.; Holaday, A.
Constitutively overexpressing a tomato fructokinase gene (LeFRK1) in cotton (Gossypium hirsutum L. cv. Coker 312) positively affects plant vegetative growth, boll number and seed cotton yield
Funct. Plant Biol.
42
899-908
2015
Solanum lycopersicum (O04897)
Stein, O.; Damari-Weissler, H.; Secchi, F.; Rachamilevitch, S.; German, M.A.; Yeselson, Y.; Amir, R.; Schaffer, A.; Holbrook, N.M.; Aloni, R.; Zwieniecki, M.A.; Granot, D.
The tomato plastidic fructokinase SlFRK3 plays a role in xylem development
New Phytol.
209
1484-1495
2016
Solanum lycopersicum (Q6VWJ5), Solanum lycopersicum
Stein, O.; Secchi, F.; German, M.; Damari-Weissler, H.; Aloni, R.; Holbrook, N.; Zwieniecky, M.; Granot, D.
The tomato cytosolic fructokinase FRK1 is important for phloem fiber development
Biol. Plant.
62
353-361
2018
Solanum lycopersicum, Solanum lycopersicum (O04897), Solanum lycopersicum (Q42896)
-
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