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Information on EC 2.7.1.39 - homoserine kinase and Organism(s) Arabidopsis thaliana and UniProt Accession Q8L7R2

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Arabidopsis thaliana
UNIPROT: Q8L7R2 not found.
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
hsk, homoserine kinase, thr1p, atp:l-homoserine o-phosphotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homoserine kinase
-
homoserine kinase (phosphorylating)
-
-
-
-
HSK
-
-
-
-
kinase (phosphorylating), homoserine
-
-
-
-
kinase, homoserine (phosphorylating)
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:L-homoserine O-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9026-58-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + L-homoserine
ADP + O-phospho-L-homoserine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-homoserine
ADP + O-phospho-L-homoserine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
requires 3 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
L-homoserine
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.09
L-homoserine-dependent ADP synthesis assay at pH 8.5 and 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
soluble in stromal fraction
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
mutations in the Arabidopsis homoserine kinase gene DMR1 confer enhanced resistance to Fusarium culmorum and Fusarium graminearum which cause Fusarium Ear Blight disease on small grain cereals
metabolism
downy mildew resistance is mediated by mutation of homoserine kinase: homoserine accumulation in the chloroplast triggers a novel form of downy mildew resistance
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KHSE_ARATH
370
0
38529
Swiss-Prot
Chloroplast (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme stored with 3 mM ATP loses 50% of its activity after two freezing cycles. In presence of 50% v/v glycerol the enzyme loses 50% of its activity after 10 cycles. In the basic storage buffer, the enzyme loses 80% of its activity after a single freeze-thaw cycle
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of a His-tagged construct in Escherichia coli
His-tag, expressed in Escherichia coli
overexpressed in Arabidopsis thaliana
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lee, M.; Leustek, T.
Identification of the gene encoding homoserine kinase from Arabidopsis thaliana and characterization of the recombinant enzyme derived from the gene
Arch. Biochem. Biophys.
372
135-142
1999
Arabidopsis thaliana (Q8L7R2), Arabidopsis thaliana
Manually annotated by BRENDA team
Lee, M.; Martin, M.N.; Hudson, A.O.; Lee, J.; Muhitch, M.J.; Leustek, T.
Methionine and threonine synthesis are limited by homoserine availability and not the activity of homoserine kinase in Arabidopsis thaliana
Plant J.
41
685-696
2005
Arabidopsis thaliana (Q8L7R2), Arabidopsis thaliana
Manually annotated by BRENDA team
van Damme, M.; Zeilmaker, T.; Elberse, J.; Andel, A.; de Sain-van der Velden, M.; van den Ackerveken, G.
Downy mildew resistance in Arabidopsis by mutation of homoserine kinase
Plant Cell
21
2179-2189
2009
Arabidopsis thaliana (Q8L7R2), Arabidopsis thaliana
Manually annotated by BRENDA team
Brewer, H.C.; Hawkins, N.D.; Hammond-Kosack, K.E.
Mutations in the Arabidopsis homoserine kinase gene DMR1 confer enhanced resistance to Fusarium culmorum and F. graminearum
BMC Plant Biol.
14
317
2014
Arabidopsis thaliana (Q8L7R2)
Manually annotated by BRENDA team