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Information on EC 2.7.1.36 - mevalonate kinase and Organism(s) Methanosarcina mazei and UniProt Accession Q8PW39

for references in articles please use BRENDA:EC2.7.1.36
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EC Tree
IUBMB Comments
CTP, GTP and UTP can also act as donors.
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This record set is specific for:
Methanosarcina mazei
UNIPROT: Q8PW39
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The taxonomic range for the selected organisms is: Methanosarcina mazei
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
mevalonate kinase, mvk, mva kinase, tcmvk, mevalonic kinase, temvk, mevalonate phosphokinase, mevalonate 5-phosphotransferase, atp:mevalonate 5-phosphotransferase, mevalonic acid kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ATP:mevalonate 5-phosphotransferase
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kinase, mevalonate (phosphorylating)
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mevalonate 5-phosphotransferase
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mevalonate phosphokinase
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mevalonic acid kinase
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mevalonic kinase
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MVA kinase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:(R)-mevalonate 5-phosphotransferase
CTP, GTP and UTP can also act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-52-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (R)-mevalonate
ADP + (R)-5-phosphomevalonate
show the reaction diagram
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?
ATP + (R)-mevalonate
ADP + (R)-5-phosphomevalonate
show the reaction diagram
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?
additional information
?
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analysis of substrate and product binding by archaeal mevalonate kinase compared to eukaryotic enzymes, a striking lack of direct interactions between this archaeal MK and its substrate is detected, substrate-binding site structure, overview
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (R)-mevalonate
ADP + (R)-5-phosphomevalonate
show the reaction diagram
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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the enzyme is not inhibited by dimethylallyl diphosphate, geranyl diphosphate, farnesyl diphosphate, isopentenyl monophosphate, and diphosphomevalonate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.068
(R)-mevalonate
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apparent value, in 50 mM Tris, 50 mM NaCl (pH 7.6), at 30°C
0.464
ATP
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apparent value, in 50 mM Tris, 50 mM NaCl (pH 7.6), at 30°C
additional information
additional information
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Michaelis-Menten kinetics
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.3
(R)-mevalonate
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in 50 mM Tris, 50 mM NaCl (pH 7.6), at 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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mevalonate kinase (MK) is a key enzyme of the mevalonate pathway, which produces the biosynthetic precursors for steroids, including cholesterol, and isoprenoids, the largest class of natural products
additional information
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structure comparisons of MVKs from different species, active site structure and structure-function analysis, detailed overview
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
2 * 33000, SDS-PAGE
33600
2 * 33600, His-tagged protein, calculated from amino acid sequence
64600
gel filtration
35500
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2 * 35500, calculated from amino acid sequence
78000
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gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
homodimer
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2 * 35500, calculated from amino acid sequence
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.32 M MgCl2, 0.08 M bis-Tris pH 5.5, 16% (w/v) PEG 3350, at 18°C
purified enzyme in mevalonate-bound and 5-phosphomevalonate-bound form, hanging drop vapor diffusion method, mixing of 11 mg/ml protein in 20 mM Tris pH 7.7, 10 mM MgCl2, and 10% glycerol, with 100 mM Bis-Tris pH 5.5, 200 mM sodium potassium tartrate, and 15-25% PEG 8000 or 10000, microseeding. Seeded drops contain 0.001 ml of protein with 1 mM (R)-mevalonate, 5 mM AMPPNP, and 5 mM MgCl2, and 800 nl crystallization solution, and 200 nl of the seed stock. Diffraction-quality plate crystals grow in crystallization solutions that contain 100 mM Bis-Tris, pH 5.5, 200-300 mM sodium potassium tartrate, and 15-25% PEG 8000 or 10000, X-ray diffraction structure determination and analysis at 2.1-2.46 A resolution, molecular replacement using the structure of the apo-MmMK from Methanosarcina mazei (PDB ID 4HAC) as the search model
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA bead column chromatography, Resource 15Q column chromatography, and Superdex 200 gel filtration
HiTrap IMAC column chromatography and HiTrap Q column chromatography
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21 Star (lambdaDE3) cells
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recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10B
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhuang, N.; Seo, K.H.; Chen, C.; Zhou, J.; Kim, S.W.; Lee, K.H.
Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazei
Acta Crystallogr. Sect. F
68
1560-1563
2012
Methanosarcina mazei (Q8PW39), Methanosarcina mazei
Manually annotated by BRENDA team
Primak, Y.A.; Du, M.; Miller, M.C.; Wells, D.H.; Nielsen, A.T.; Weyler, W.; Beck, Z.Q.
Characterization of a feedback-resistant mevalonate kinase from the archaeon Methanosarcina mazei
Appl. Environ. Microbiol.
77
7772-7778
2011
Methanosarcina mazei
Manually annotated by BRENDA team
Miller, B.R.; Kung, Y.
Structural insight into substrate and product binding in an archaeal mevalonate kinase
PLoS ONE
13
e0208419
2018
Methanosarcina mazei
Manually annotated by BRENDA team