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Information on EC 2.7.1.35 - pyridoxal kinase and Organism(s) Rattus norvegicus and UniProt Accession O35331

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IUBMB Comments
Pyridoxine, pyridoxamine and various derivatives can also act as acceptors.
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This record set is specific for:
Rattus norvegicus
UNIPROT: O35331
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
pyridoxal kinase, pyridoxine kinase, pl kinase, ldpdxk, pyridoxal phosphokinase, stplk, pn kinase, hpl kinase, epl kinase 1, pn/pl/pm kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pyridoxal kinase
-
kinase (phosphorylating), pyridoxal
-
-
-
-
kinase, pyridoxal (phosphorylating)
-
-
-
-
PL kinase
-
-
-
-
PLK
-
-
-
-
PM kinase
-
-
-
-
PN kinase
-
-
-
-
PN/PL/PM kinase
-
-
-
-
pyridoxal 5-phosphate-kinase
-
-
-
-
pyridoxal kinase
-
-
-
-
pyridoxal kinase-like protein SOS4
-
-
-
-
pyridoxal phosphokinase
-
-
-
-
pyridoxamine kinase
-
-
-
-
pyridoxine kinase
-
-
-
-
pyridoxine/pyridoxal/pyridoxamine kinase
-
-
-
-
vitamin B6 kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:pyridoxal 5'-phosphotransferase
Pyridoxine, pyridoxamine and various derivatives can also act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-42-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 4-deoxypyridoxine
ADP + 4-deoxypyridoxine 5'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + 5-deoxypyridoxal
ADP + ?
show the reaction diagram
-
-
-
-
?
ATP + omega-methylpyridoxal
ADP + omega-methylpyridoxal 5'-phosphate
show the reaction diagram
-
-
-
-
?
ATP + pyridoxal
ADP + pyridoxal 5'-phosphate
show the reaction diagram
ATP + pyridoxine
ADP + pyridoxine 5'-phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
0.2 mM, activates
Mn2+
-
0.2 mM, partial activation
Zn2+
-
0.2 mM, activates
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,6-Dimethyl-3,4-bis(hydroxymethyl)pyridine
-
-
2-Methyl-3-amino-4,5,6-tris(hydroxymethyl)pyridine
-
-
2-Methyl-3-amino-4,5-bis(hydroxymethyl)pyridine
-
-
2-Methyl-3-hydroxy-5,6-bis(hydroxymethyl)pyridine
-
-
2-Methyl-4,5-bis(hydroxymethyl)pyridine
-
-
5-hydroxytryptamine
-
0.5 mM, 11% inhibition of activity with pyridoxine, 81% inhibition of activity with pyridoxal
alpha-Methylphenethylhydrazine
-
0.015 mM, 50% inhibition
alpha-Methylphenethylhydrazone
-
0.015 mM, 50% inhibition
azine
-
0.0004 mM, 50% inhibition
cadaverine
-
-
histamine
-
-
hydrazine
-
0.05 mM, 50% inhibition
hydroxylamine
-
0.01 mM, 50% inhibition
NADH
-
0.5 mM, 14% inhibition of activity with pyridoxine, 11% inhibition of activity with pyridoxal
Oxime
-
0.008 mM, 50% inhibition
picolinate
-
0.5 mM, 95% inhibition of activity with pyridoxine, 97% inhibition of activity with pyridoxal
putrescine
-
-
pyridoxine
-
substrate inhibition above 0.2 mM
quinolinate
-
0.5 mM, 82% inhibition of activity with pyridoxine, 82% inhibition of activity with pyridoxal
Semicarbazine
-
0.0005 mM, 50% inhibition
serotonin
-
0.08 mM, 50% inhibition
spermidine
-
weak
spermine
-
weak
tryptamine
tyramine
-
-
xanthurenate
-
0.5 mM, 19% inhibition of activity with pyridoxine, 18% inhibition of activity with pyridoxal
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
-
0.5 mM, activation to 125%, activity with pyridoxine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
pyridoxal
-
37°C
0.12
pyridoxine
-
37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
AMP
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.004
-
brain enzyme
0.0125
-
liver enzyme
1.27
-
reaction with pyridoxal
1.42
-
reaction with pyridoxine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
dentate granule cell. Pyridoxal kinase immunoreactivity is not increased after induction of long-term potentiation and not involved in increase in the efficiency of high frequency stimulus-induced potentiation of populations spike amplitude when compared with saline-, or Tat-protein-treated animals
Manually annotated by BRENDA team
-
weak activity
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PDXK_RAT
312
0
34908
Swiss-Prot
other Location (Reliability: 3)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Takeuchi, F.; Tsubouchi, R.; Shibata, Y.
Effect of tryptophan metabolites on the activities of rat liver pyridoxal kinase and pyridoxamine 5-phosphate oxidase in vitro
Biochem. J.
227
537-544
1985
Rattus norvegicus
Manually annotated by BRENDA team
McCormick, D.B.; Gregory, M.E.; Snell, E.E.
Pyridoxal phosphokinases. I. Assay, distribution, purification, and properties
J. Biol. Chem.
236
2076-2084
1961
Bos taurus, Enterococcus faecalis, Homo sapiens, Lacticaseibacillus casei, Lactobacillus delbrueckii subsp. bulgaricus, Meleagris gallopavo, Mus musculus, Neurospora crassa, Oryctolagus cuniculus, Rattus norvegicus, Saccharomyces cerevisiae
Manually annotated by BRENDA team
McCormick, D.B.; Snell, E.E.
Pyridoxal phosphokinase. II. Effects of inhibitors
J. Biol. Chem.
236
2085-2088
1961
Bos taurus, Enterococcus faecalis, Lacticaseibacillus casei, Rattus norvegicus, Saccharomyces pastorianus
Manually annotated by BRENDA team
Gaeng, V.; von Collins, E.N.
Inhibition of pyridoxal kinase from rat liver in vitro by aromatic and aliphatic amines
Int. J. Vitam. Nutr. Res.
43
318-323
1973
Rattus norvegicus
Manually annotated by BRENDA team
Kwak, S.E.; Kim, J.E.; Kim, D.W.; Kwon, O.S.; Choi, S.Y.; Kang, T.C.
Pyridoxine 5-phosphate oxidase, not pyridoxal kinase, involves in long-term potentiation induction in the rat dentate gyrus
Hippocampus
19
45-56
2009
Rattus norvegicus
Manually annotated by BRENDA team
Kwak, S.E.; Kim, J.E.; Kim, D.W.; Kwon, O.S.; Choi, S.Y.; Kang, T.C.
Enhanced pyridoxal 5-phosphate synthetic enzyme immunoreactivities do not contribute to GABAergic inhibition in the rat hippocampus following pilocarpine-induced status epilepticus
Neuroscience
159
1108-1118
2009
Rattus norvegicus (O35331)
Manually annotated by BRENDA team