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Information on EC 2.7.1.33 - pantothenate kinase and Organism(s) Thermotoga maritima and UniProt Accession Q9WZY5

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Thermotoga maritima
UNIPROT: Q9WZY5 not found.
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Word Map
The taxonomic range for the selected organisms is: Thermotoga maritima
The enzyme appears in selected viruses and cellular organisms
Synonyms
pank2, pantothenate kinase, pantothenate kinase 2, pank1, pank3, pank4, pank-iii, pantothenate kinase-2, type ii pantothenate kinase, pfpank1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-pantothenate kinase
-
-
-
-
hPanK
-
-
-
-
hPanK1
-
-
-
-
hPANK2
-
-
-
-
hPanK3
-
-
-
-
hPanK4
-
-
-
-
kinase, pantothenate (phosphorylating)
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-
-
-
mPank
-
-
-
-
mPank1
-
-
-
-
mPanK3
-
-
-
-
PAK
-
-
-
-
pantothenic acid kinase
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-
-
-
rPanK4
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-
-
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Rts protein
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-
-
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type III pantothenate kinase
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
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-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:(R)-pantothenate 4'-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9026-48-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + pantothenate
ADP + (R)-4'-phosphopantothenate
show the reaction diagram
-
-
-
?
ATP + (R)-pantothenate
ADP + (R)-4'-phosphopantothenate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
not inhibited by CoA or its thioesters
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.043
(R)-pantothenate
-
in 100 mM HEPES, pH 7.6, 20 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 units of lactate dehydrogenase, 2.5 units of pyruvate kinase, and 0.00027 mM of the PanK-III protein, at 50°C
6.04
ATP
-
in 100 mM HEPES, pH 7.6, 20 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 units of lactate dehydrogenase, 2.5 units of pyruvate kinase, and 0.00027 mM of the PanK-III protein, at 50°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29
(R)-pantothenate
-
in 100 mM HEPES, pH 7.6, 20 mM KCl, 10 mM MgCl2, 2 mM phosphoenolpyruvate, 0.3 mM NADH, 5 units of lactate dehydrogenase, 2.5 units of pyruvate kinase, and 0.00027 mM of the PanK-III protein, at 50°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
gel filtration, functional unit in solution
hexamer
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X-ray crystallography, in the crystal form
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor-diffusion method, crystal structures of PanK-III in complex with pantothenate, phosphopantothenate as well as a ternary complex structure of PanK-III with pantothenate and ADP
sitting drop vapour diffusion method with 15% polyethylene glycol 3350
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D105E
-
less than 6% activity of the wild type enzyme
D105N
-
less than 6% activity of the wild type enzyme
D125E
-
less than 6% activity of the wild type enzyme
D125N
-
less than 6% activity of the wild type enzyme
D6E
-
less than 6% activity of the wild type enzyme
D6N
-
less than 6% activity of the wild type enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
nickel-nitrilotriacetic acid-agarose column chromatography and Resource Q column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BL21(DE3)
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, K.; Eyobo, Y.; Brand, L.A.; Martynowski, D.; Tomchick, D.; Strauss, E.; Zhang, H.
Crystal structure of a type III pantothenate kinase: insight into the mechanism of an essential coenzyme A biosynthetic enzyme universally distributed in bacteria
J. Bacteriol.
188
5532-5540
2006
Thermotoga maritima
Manually annotated by BRENDA team
Yang, K.; Strauss, E.; Huerta, C.; Zhang, H.
Structural basis for substrate binding and the catalytic mechanism of type III pantothenate kinase
Biochemistry
47
1369-1380
2008
Helicobacter pylori, Thermotoga maritima (Q9WZY5), Thermotoga maritima
Manually annotated by BRENDA team