Information on EC 2.7.1.32 - choline kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.1.32
-
RECOMMENDED NAME
GeneOntology No.
choline kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + choline = ADP + phosphocholine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
Phosphorylation
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycerophospholipid metabolism
-
-
Metabolic pathways
-
-
phosphatidylcholine biosynthesis I
-
-
phosphatidylethanolamine bioynthesis
-
-
plasmalogen biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:choline phosphotransferase
Ethanolamine and its methyl and ethyl derivatives can also act as acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-67-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Columbia
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
canola
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
African green monkey
-
-
Manually annotated by BRENDA team
pigeon
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
IFO 0987
-
-
Manually annotated by BRENDA team
chicken
-
-
Manually annotated by BRENDA team
soy bean
-
-
Manually annotated by BRENDA team
Hemidactylus sp.
lizard
-
-
Manually annotated by BRENDA team
catfish
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain KS106
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
protozoan parasite which causes African sleeping sickness
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-(dimethylamino)-ethanol + ATP
ADP + 2-(dimethylamino)-ethyl phosphate
show the reaction diagram
2-aminoethanol + ATP
ADP + 2-aminoethyl phosphate
show the reaction diagram
-
-
-
-
?
2-methylaminoethanol + ATP
ADP + 2-methylaminoethyl phosphate
show the reaction diagram
-
-
-
-
?
ATP + choline
ADP + O-phosphocholine
show the reaction diagram
ATP + choline
ADP + phosphocholine
show the reaction diagram
ATP + dimethylethanolamine
ADP + phosphodimethylethanolamine
show the reaction diagram
-
-
-
-
?
ATP + ethanolamine
ADP + ethanolamine phosphate
show the reaction diagram
ATP + ethanolamine
ADP + phosphoethanolamine
show the reaction diagram
-
-
-
-
?
beta-methylcholine + ATP
ADP + beta-methylcholine phosphate
show the reaction diagram
-
-
-
-
?
choline + ATP
O-phosphocholine + ADP
show the reaction diagram
choline + ATP
phosphocholine + ADP
show the reaction diagram
choline + ATP
phosphorylcholine + ADP
show the reaction diagram
-
-
-
?
CTP + choline
CDP + choline phosphate
show the reaction diagram
-
12% of the activity with ATP
-
-
?
diethanolamine + ATP
ADP + O-phospho-diethanolamine
show the reaction diagram
-
-
-
-
?
ethanolamine + ATP
ADP + ethanolamine phosphate
show the reaction diagram
ethanolamine + ATP
O-phosphoethanolamine + ADP
show the reaction diagram
-
first step in the biosynthesis of the glycerophospholipid glycerophosphoethanolamine
-
-
?
ethanolamine + ATP
phosphoethanolamine + ADP
show the reaction diagram
-
-
-
-
?
GTP + choline
GDP + choline phosphate
show the reaction diagram
N,N,N-triethylethanolamine + ATP
ADP + N,N,N-triethylethanolamine phosphate
show the reaction diagram
-
weak
-
-
?
N,N-diethyl-N-methylethanolamine + ATP
ADP + N,N-diethyl-N-methylethanolamine phosphate
show the reaction diagram
-
weak
-
-
?
N,N-diethylethanolamine + ATP
ADP + N,N-diethylethanolamine phosphate
show the reaction diagram
N,N-diethylethanolamine + ATP
ADP + O-phospho-N-diethylethanolamine
show the reaction diagram
-
-
-
-
?
N,N-diisopropylethanolamine + ATP
ADP + N,N-diisopropylethanolamine phosphate
show the reaction diagram
-
-
-
-
?
N,N-dimethyl-N-propylethanolamine + ATP
ADP + N,N-dimethyl-N-propylethanolamine phosphate
show the reaction diagram
-
-
-
-
?
N,N-dimethylaminopropanol + ATP
ADP + N,N-dimethylaminopropyl phosphate
show the reaction diagram
-
-
-
-
?
N,N-dimethylethanolamine + ATP
ADP + N,N-dimethylethanolamine phosphate
show the reaction diagram
N,N-dimethylethanolamine + ATP
ADP + O-phospho-N,N-dimethylethanolamine
show the reaction diagram
-
-
-
-
?
N,N-dimethylethylcholine + ATP
ADP + N,N-dimethylethylcholine phosphate
show the reaction diagram
-
-
-
-
?
N,N-dimethylisopropylcholine + ATP
ADP + N,N-dimethylisopropylcholine phosphate
show the reaction diagram
-
-
-
-
?
N,N-dimethylpropylcholine + ATP
ADP + N,N-dimethylpropylcholine phosphate
show the reaction diagram
-
-
-
-
?
N-ethylethanolamine + ATP
ADP + O-phospho-N-ethylethanolamine
show the reaction diagram
-
-
-
-
?
N-methylethanolamine + ATP
ADP + N-methylethanolamine phosphate
show the reaction diagram
N-methylethanolamine + ATP
ADP + O-phospho-N-methylethanolamine
show the reaction diagram
-
-
-
-
?
N-monoethylethanolamine + ATP
ADP + N-monoethylethanolamine phosphate
show the reaction diagram
-
-
-
-
?
N-monomethylethanolamine + ATP
ADP + N-monomethylethanolamine phosphate
show the reaction diagram
UTP + choline
UDP + choline phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + choline
ADP + O-phosphocholine
show the reaction diagram
choline + ATP
O-phosphocholine + ADP
show the reaction diagram
-
first step in the biosynthesis of the glycerophospholipid glycerophosphocholine
-
-
?
choline + ATP
phosphocholine + ADP
show the reaction diagram
ethanolamine + ATP
O-phosphoethanolamine + ADP
show the reaction diagram
-
first step in the biosynthesis of the glycerophospholipid glycerophosphoethanolamine
-
-
?
additional information
?
-
-
microarray analysis detected changes in the expression of 33 proliferation-related genes with Chk down-regulation
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Carpronium chloride
-
stimulates
Chlorocholine chloride
-
stimulates
Li+
-
stimulates
MgCl2
-
assay at
NH4+
-
stimulates
additional information
-
Co2+, Cd2+: almost completely ineffective, Ca2+, Ni2+, Zn2+: totally ineffective
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,1',1''-(benzene-1,3,5-triylmethylene)tris[(4-dimethylamino)pyridinium] tribromide
-
-
1,1',1''-(benzene-1,3,5-triylmethylene)tris[4-(3,5-dichloro-N-methylanilino)pyridinium] tribromide
-
-
1,1',1''-(benzene-1,3,5-triylmethylene)tris[4-(4-chloro-N-methylanilino)pyridinium] tribromide
-
-
1,1',1''-(benzene-1,3,5-triylmethylene)tris[4-(N-methylanilino)pyridinium] tribromide
-
-
1,1',1''-(benzene-1,3,5-triylmethylene)tris[4-(piperidino)pyridinium] tribromide
-
-
1,1',1''-(benzene-1,3,5-triylmethylene)tris[4-(pyrrolidino)pyridinium] tribromide
-
-
1,1'-(benzene-1,3-diyldimethanediyl)bis(4-piperidin-1-ylpyridinium) dibromide
-
IC50: 0.0528 mM
1,1'-(benzene-1,3-diyldimethanediyl)bis(4-pyrrolidin-1-ylpyridinium) dibromide
-
IC50: 0.0842 mM
1,1'-(benzene-1,3-diyldimethanediyl)bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
-
IC50: 0.0084 mM
1,1'-(benzene-1,3-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]pyridinium) dibromide
-
IC50: 0.0072 mM
1,1'-(benzene-1,3-diyldimethanediyl)bis[4-[(phenylamino)methyl]pyridinium] dibromide
-
IC50: 0.0375 mM
1,1'-(benzene-1,3-diylmethylene)bis[(4-dimethylamino)pyridinium] dibromide
-
-
1,1'-(benzene-1,3-diylmethylene)bis[4-(N-methylanilino)pyridinium] dibromide
-
-
1,1'-(benzene-1,3-diylmethylene)bis[4-(piperidino)pyridinium] dibromide
-
-
1,1'-(benzene-1,3-diylmethylene)bis[4-(pyrrolidino)pyridinium] dibromide
-
-
1,1'-(benzene-1,4-diyldimethanediyl)bis(4-piperidin-1-ylpyridinium) dibromide
-
IC50: 0.0377 mM
1,1'-(benzene-1,4-diyldimethanediyl)bis(4-pyrrolidin-1-ylpyridinium) dibromide
-
IC50: 0.024 mM
1,1'-(benzene-1,4-diyldimethanediyl)bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
-
IC50: 0.0097 mM
1,1'-(benzene-1,4-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]pyridinium) dibromide
-
IC50: 0.0029 mM
1,1'-(benzene-1,4-diyldimethanediyl)bis[4-[(phenylamino)methyl]pyridinium] dibromide
-
IC50: 0.015 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-amino-3-methylquinolinium) dibromide
-
IC50: 0.0119 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-amino-7-chloroquinolinium) dibromide
-
IC50: 0.0206 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-aminoquinolinium) dibromide
-
IC50: 0.0012 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-azepan-1-ylquinolinium) dibromide
-
IC50: 0.0005 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-piperidin-1-ylpyridinium) dibromide
-
IC50: 0.0019 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-pyrrolidin-1-ylpyridinium) dibromide
-
IC50: 0.0058 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
-
IC50: 0.0026 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]pyridinium) dibromide
-
IC50: 0.0015 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
-
IC50: 0.0021 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(7-amino-4-[[(4-chlorophenyl)amino]methyl]-8-methylquinolinium) dibromide
-
IC50: 0.147 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(7-chloro-4-pyrrolidin-1-ylquinolinium) dibromide
-
IC50: 0.0012 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(7-chloro-4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
-
IC50: 0.0057 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[4-(dimethylamino)quinolinium] dibromide
-
IC50: 0.0044 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[4-(phenylamino)quinolinium] dibromide
-
IC50: 0.0013 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[4-[(phenylamino)methyl]pyridinium] dibromide
-
IC50: 0.00043 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[4-[(phenylamino)methyl]quinolinium] dibromide
-
IC50: 0.0004 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[7-amino-8-methyl-4-[(phenylamino)methyl]quinolinium] dibromide
-
IC50: 0.0568 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[7-chloro-4-(dimethylamino)quinolinium] dibromide
-
IC50: 0.0096 mM
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[7-chloro-4-[(phenylamino)methyl]quinolinium] dibromide
-
IC50: 0.0031 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-amino-3-methylquinolinium) dibromide
-
IC50: above 0.2 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-amino-7-chloroquinolinium) dibromide
-
IC50: 0.0633 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-aminoquinolinium) dibromide
-
IC50: 0.0811 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-azepan-1-ylquinolinium) dibromide
-
IC50: 0.0022 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-piperidin-1-ylpyridinium) dibromide
-
IC50: 0.0088 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-pyrrolidin-1-ylpyridinium) dibromide
-
IC50: 0.0111 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
-
IC50: 0.0034 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]pyridinium) dibromide
-
IC50: 0.0018 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
-
IC50: 0.002 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(7-amino-4-[[(4-chlorophenyl)amino]methyl]-8-methylquinolinium) dibromide
-
IC50: 0.0461 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(7-chloro-4-pyrrolidin-1-ylquinolinium) dibromide
-
IC50: 0.0198 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(7-chloro-4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
-
IC50: 0.0114 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[4-(dimethylamino)quinolinium] dibromide
-
IC50: 0.0397 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[4-(phenylamino)quinolinium] dibromide
-
IC50: 0.0178 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[4-[(phenylamino)methyl]pyridinium] dibromide
-
IC50: 0.0043 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[4-[(phenylamino)methyl]quinolinium] dibromide
-
IC50: 0.003 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[7-amino-8-methyl-4-[(phenylamino)methyl]quinolinium] dibromide
-
IC50: 0.0961 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[7-chloro-4-(dimethylamino)quinolinium] dibromide
-
IC50: 0.0206 mM
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[7-chloro-4-[(phenylamino)methyl]quinolinium] dibromide
-
IC50: 0.0114 mM
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis(4-aminoquinolinium)
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis(4-azepan-1-ylquinolinium)
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[4-(dimethylamino)quinolinium]
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[4-(phenylamino)quinolinium]
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[4-[(4-chlorophenyl)(methyl)amino]quinolinium]
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[4-[methyl(phenyl)amino]quinolinium]
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-amino-4-[(4-chlorophenyl)(methyl)amino]-8-methylquinolinium]
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-amino-8-methyl-4-[methyl(phenyl)amino]quinolinium]
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-chloro-4-(dimethylamino)quinolinium]
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-chloro-4-[(4-chlorophenyl)(methyl)amino]quinolinium]
-
-
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-chloro-4-[methyl(phenyl)amino]quinolinium]
-
-
1,1'-biphenyl-3,3'-diylbis(4-amino-3-methylquinolinium)
-
-
1,1'-biphenyl-3,3'-diylbis(4-amino-7-chloroquinolinium)
-
-
1,1'-biphenyl-3,3'-diylbis(4-aminoquinolinium)
-
-
1,1'-biphenyl-3,3'-diylbis(4-azepan-1-ylquinolinium)
-
-
1,1'-biphenyl-3,3'-diylbis(7-chloro-4-pyrrolidin-1-ylquinolinium)
-
-
1,1'-biphenyl-3,3'-diylbis[4-(dimethylamino)quinolinium]
-
-
1,1'-biphenyl-3,3'-diylbis[4-(phenylamino)quinolinium]
-
-
1,1'-biphenyl-3,3'-diylbis[4-[(4-chlorophenyl)(methyl)amino]quinolinium]
-
-
1,1'-biphenyl-3,3'-diylbis[4-[methyl(phenyl)amino]quinolinium]
-
-
1,1'-biphenyl-3,3'-diylbis[7-amino-4-[(4-chlorophenyl)(methyl)amino]-8-methylquinolinium]
-
-
1,1'-biphenyl-3,3'-diylbis[7-amino-8-methyl-4-[methyl(phenyl)amino]quinolinium]
-
-
1,1'-biphenyl-3,3'-diylbis[7-chloro-4-(dimethylamino)quinolinium]
-
-
1,1'-biphenyl-3,3'-diylbis[7-chloro-4-[(4-chlorophenyl)(methyl)amino]quinolinium]
-
-
1,1'-biphenyl-3,3'-diylbis[7-chloro-4-[methyl(phenyl)amino]quinolinium]
-
-
1,1'-biphenyl-4,4'-diylbis(4-amino-7-chloroquinolinium)
-
-
1,1'-biphenyl-4,4'-diylbis(4-aminoquinolinium)
-
-
1,1'-biphenyl-4,4'-diylbis(4-azepan-1-ylquinolinium)
-
-
1,1'-biphenyl-4,4'-diylbis(7-chloro-4-pyrrolidin-1-ylquinolinium)
-
-
1,1'-biphenyl-4,4'-diylbis[4-(dimethylamino)quinolinium]
-
-
1,1'-biphenyl-4,4'-diylbis[4-(phenylamino)quinolinium]
-
-
1,1'-biphenyl-4,4'-diylbis[4-[(4-chlorophenyl)(methyl)amino]quinolinium]
-
-
1,1'-biphenyl-4,4'-diylbis[4-[methyl(phenyl)amino]quinolinium]
-
-
1,1'-biphenyl-4,4'-diylbis[7-amino-4-[(4-chlorophenyl)(methyl)amino]-8-methylquinolinium]
-
-
1,1'-biphenyl-4,4'-diylbis[7-amino-8-methyl-4-[methyl(phenyl)amino]quinolinium]
-
-
1,1'-biphenyl-4,4'-diylbis[7-chloro-4-(dimethylamino)quinolinium]
-
-
1,1'-biphenyl-4,4'-diylbis[7-chloro-4-[(4-chlorophenyl)(methyl)amino]quinolinium]
-
-
1,1'-biphenyl-4,4'-diylbis[7-chloro-4-[methyl(phenyl)amino]quinolinium]
-
-
1,1'-[(1S,2S)-cyclopropane-1,2-diyldiethane-2,1-diyl]bis(4-piperidin-1-ylpyridinium) dibromide
-
IC50: 0.098 mM
1,1'-[(1S,2S)-cyclopropane-1,2-diyldiethane-2,1-diyl]bis(4-pyrrolidin-1-ylpyridinium) dibromide
-
IC50: above 0.1 mM
1,1'-[(1S,2S)-cyclopropane-1,2-diyldiethane-2,1-diyl]bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
-
IC50: 0.0482 mM; IC50: above 0.1 mM
1,1'-[(1S,2S)-cyclopropane-1,2-diyldiethane-2,1-diyl]bis[4-[(phenylamino)methyl]pyridinium] dibromide
-
IC50: above 0.1 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(4-amino-7-chloroquinolinium) dibromide
-
IC50: above 0.2 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(4-aminoquinolinium) dibromide
-
IC50: 0.08 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(4-azepan-1-ylquinolinium) dibromide
-
IC50: 0.006 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
-
IC50: 0.0048 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(7-amino-4-[[(4-chlorophenyl)amino]methyl]-8-methylquinolinium) dibromide
-
IC50: 0.0575 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(7-chloro-4-pyrrolidin-1-ylquinolinium) dibromide
-
IC50: 0.001 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(7-chloro-4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
-
IC50: 0.0057 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[4-(dimethylamino)quinolinium] dibromide
-
IC50: 0.0102 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[4-(phenylamino)quinolinium] dibromide
-
IC50: 0.0023 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[4-[(phenylamino)methyl]quinolinium] dibromide
-
IC50: 0.0014 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[7-amino-8-methyl-4-[(phenylamino)methyl]quinolinium] dibromide
-
IC50: 0.133 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[7-chloro-4-(dimethylamino)quinolinium] dibromide
-
IC50: 0.009 mM
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[7-chloro-4-[(phenylamino)methyl]quinolinium] dibromide
-
IC50: 0.0035 mM
1-(4-[4-[(6-amino-3H-purin-3-yl)methyl]phenethyl]benzyl)-4-pyrrolidinopyridinium bromide
0.1% inhibition at 0.01 mM, 24.8% inhibition at 0.05 mM
1-(4-[4-[(6-amino-3H-purin-3-yl)methyl]phenyl]benzyl)-4-(dimethylamino)pyridinium bromide
62.3% inhibition at 0.01 mM, 71.4% inhibition at 0.05 mM
1-(4-[4-[(6-amino-9H-purin-9-yl)methyl]phenethyl]benzyl)-4-(dimethylamino)pyridinium bromide
38.6% inhibition at 0.01 mM, 81% inhibition at 0.05 mM
1-(4-[4-[(6-amino-9H-purin-9-yl)methyl]phenethyl]benzyl)-4-pyrrolidinopyridinium bromide
28.6% inhibition at 0.01 mM, 46.9% inhibition at 0.05 mM
1-(4-[4-[(6-amino-9H-purin-9-yl)methyl]phenyl]benzyl)-4-(dimethylamino)pyridinium bromide
37.6% inhibition at 0.01 mM, 51% inhibition at 0.05 mM
1-(4-[4-[(6-amino-9H-purin-9-yl)methyl]phenyl]benzyl)-4-pyrrolidinopyridinium bromide
50.2% inhibition at 0.01 mM, 72.8% inhibition at 0.05 mM
1-[4-(4-[4-[(6-amino-3H-purin-3-yl)methyl]phenyl]butyl)benzyl]-4-(dimethylamino)pyridinium bromide
61.3% inhibition at 0.01 mM, 68.2% inhibition at 0.05 mM
1-[4-(4-[4-[(6-amino-3H-purin-3-yl)methyl]phenyl]butyl)benzyl]-4-pyrrolidinopyridinium bromide
22.6% inhibition at 0.01 mM, 59.9% inhibition at 0.05 mM
1-[4-(4-[4-[(6-amino-9H-purin-8-yl)methyl]phenyl]butyl)benzyl]-4-(dimethylamino)pyridinium bromide
-
-
1-[4-(4-[4-[(6-amino-9H-purin-9-yl)methyl]phenyl]butyl)benzyl]-4-(dimethylamino)pyridinium bromide
66.7% inhibition at 0.01 mM, 89.7% inhibition at 0.05 mM
1-[4-(4-[4-[(6-amino-9H-purin-9-yl)methyl]phenyl]butyl)benzyl]-4-pyrrolidinopyridinium bromide
36.9% inhibition at 0.01 mM, 41.9% inhibition at 0.05 mM
1-[4-[(6-amino-3H-purin-3-yl)methyl]benzyl]-4-(dimethylamino)pyridinium bromide
25% inhibition at 0.01 mM, 50.2% inhibition at 0.05 mM
1-[4-[(6-amino-9H-purin-9-yl)methyl]benzyl]-4-(dimethylamino)pyridinium bromide
46.7% inhibition at 0.01 mM, 54.1% inhibition at 0.05 mM
1-[4-[(6-amino-9H-purin-9-yl)methyl]benzyl]-4-pyrrolidinopyridinium bromide
27% inhibition at 0.05 mM
12,19-diaza-1,8-diazoniapentacyclo[18.2.2.23,6.28,11.214,17]triaconta-1(22),3,5,8,10,14,16,20,23,25,27,29-dodecaene dibromide
-
IC50: 0.0132 mM
12,21-diaza-1,8-diazoniapentacyclo[20.2.2.23,6.28,11.014,19]triaconta-1(24),3,5,8,10,14,16,18,22,25,27,29-dodecaene dibromide
-
IC50: 0.0021 mM
14,21-diaza-1,10-diazoniapentacyclo[20.2.2.210,13.216,19.03,8]triaconta-1(24),3,5,7,10,12,16,18,22,25,27,29-dodecaene dibromide
3-Trimethylammonio-1-propanol
-
-
4'-bispyridyl-5,5'-perfluoroalkyl-2,2'-bisoxazol
-
-
-
4-chloro-N-methylanilino
-
IC50: 0.0014 mM
5'-dithio-bis-(2-nitrobenzoic acid)
-
modest inhibitory activity
5,10,11,16,17,22-hexahydro-6,9:18,21-diethenodibenzo[g,p][1,5,10,14]tetraazacyclooctadecine-6,21-diium dibromide
-
IC50: 0.0248 mM
5,5'-dithiobis(2-nitrobenzoic acid)
5-fluorouracil
-
Investigation of the effect of combining transient siRNA-chk transfection with the anticancer drug 5-fluorouracil on cell viability and proliferation. Choline kinase down-regulation with 5-fluorouracil treatment increased cell kill in cancer cells.
7-chloro-1-(4-[2-[4-(7-chloro-5-pyrrolidin-1-ylquinolinium-1-yl)phenyl]ethyl]phenyl)-4-pyrrolidin-1-ylquinolinium
-
-
Acetylcholine
adenosine
choline
Choline analogues
choline kinase inhibitor Mn58b
choline kinase inhibitor TCD828
-
-
-
choline phosphate
-
-
cyclophane
-
-
Cytidine diphosphocholine
-
-
Diethylmethylcholine
-
-
dimethylethanolamine
-
-
ethanolamine
H-89
-
no activity on choline kinase at 5 mM ATP, decreasing the ATP concentration to 100 microM has no effect on choline kinase
HC-3
-
poor inhibition of 16% and 18% is observed at 50 microM and 150 microM, respectively, at both 100 and 250 microM choline
hemicholinium
hemicholinium-3
Hexadecyltrimethylammonium bromide
-
60% inhibition at 0.1 mM; HDTAB, structural resemblance to hexadecylphosphocholine, exhibits an antimalarial effect and inhibits choline kinase in a dose-dependent manner. For purified protein: 60% inhibition at 100 microM (in presence of 250 microM choline), 60% inhibition at 50 microM (in presence of 100 microM choline). HDTAB may compete with choline for the choline binding site of choline kinase and may offer competitive inhibition with respect to choline. The concentration of HDTAB required to inhibit Plasmodium falciparum growth by 62% was 10times lower than the concentration required to inhibit purified choline kinase by 60% in vitro
methionine
N-ethylmaleimide
N-Methyl-N,N-diisopropylethanolamine
-
-
N-Methyl-N-isopropylethanolamine
-
-
N-[2-bromocinnamyl(amino)ethyl]-5-isoquinoline sulfonamide
-
-
NaCl
-
8.7-35 mM: slight activation, 70 mM: inactivation
o-phenanthroline
-
-
p-chloromercuribenzoate
-
-
p-hydroxymercuribenzoate
-
-
phosphocholine
-
40% and 66% inhibition at 2.5 mM and 10 mM, respectively
phosphorylcholine
physostigmine
-
-
piperazine
-
modest inhibitory activity
purinyl-6-histamine
-
modest inhibitory activity
Quinacrine
-
indirect inhibitor
serine
stearoyl-CoA
Triethanolammonio-1-choline
-
-
-
V-11-023907
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Chloroethylammonium chloride
-
stimulates
Acetylcholine
-
stimulates
atropine
-
stimulates
c-Src
-
isoform CHKA2 total cellular activity and protein levels are regulated by c-Src
-
carbon tetrachloride
CCl4, major increase in the transcript for choline kinase alpha expression in livers from mice 3 h and 6 h after administration of carbon tetrachloride. CK-beta is unaffected by the carbon tetrachloride treatment. Choline kinase activity is induced in murine livers by carbon tetrachloride as a result of an increase in the expression of the mRNA for CK-alpha and Ck-alpha protein.; CK activity is induced by carbon tetrachloride as a result of an increase in the expression of the mRNA for CK alpha and CK alpha protein. The transcriptional activity of CK alpha was markedly increased 3 h and 6 h after treatment with carbon tetrachloride. Carbon tetrachloride promotes the expression of the transcription factor activator protein 1 (AP-1) that increases CK expression and activity.
Choline esters
-
activate
cysteine
-
omission of cysteine decreases the reaction rate by 40%
epidermal growth factor receptor
-
isoform CHKA2 total cellular activity and protein levels are regulated by epidermal growth factor receptor. In the presence of co-transfected epidermal growth factor receptor, the activity of wild type isoform CHKA2 is increased 2.5 fold
-
gibberellic acid
-
stimulates
isopropyl-beta-D-thiogalactoside
-
recombinant protein expression is induced with 1 mM
putrescine
-
slight stimulation
spermidine
-
stimulates
spermine
Zinc
-
intacellular zinc depletion resulted in a concentration-dependent induction of CKI1 expression
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0206 - 45
ATP
0.444
beta-Methylcholine
0.002 - 13
choline
0.94
dimethylethanolamine
-
in 100 mM Tris-HCl (pH 8.5), at 37C
0.787 - 12.01
ethanolamine
0.025
N,N-diethylethanolamine
0.4
N,N-Diisopropylethanolamine
0.208
N,N-Dimethylaminopropanol
0.022 - 0.085
N,N-dimethylethanolamine
0.118 - 3.7
N-Monomethylethanolamine
additional information
ethanolamine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.24 - 130
choline
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014
choline
-
pH 8.5, 37C
2 - 5.5
ethanolamine
0.00021 - 0.116
hemicholinium-3
3.45
phosphocholine
-
in 100 mM Tris/HCl, 6 mM MgCl2, 5 mM ATP, pH 8.8, at 37C
0.9 - 1.03
SAR97276
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0528
1,1'-(benzene-1,3-diyldimethanediyl)bis(4-piperidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: 0.0528 mM
0.0842
1,1'-(benzene-1,3-diyldimethanediyl)bis(4-pyrrolidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: 0.0842 mM
0.0084
1,1'-(benzene-1,3-diyldimethanediyl)bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
Homo sapiens
-
IC50: 0.0084 mM
0.0072
1,1'-(benzene-1,3-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]pyridinium) dibromide
Homo sapiens
-
IC50: 0.0072 mM
0.0375
1,1'-(benzene-1,3-diyldimethanediyl)bis[4-[(phenylamino)methyl]pyridinium] dibromide
Homo sapiens
-
IC50: 0.0375 mM
0.0377
1,1'-(benzene-1,4-diyldimethanediyl)bis(4-piperidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: 0.0377 mM
0.024
1,1'-(benzene-1,4-diyldimethanediyl)bis(4-pyrrolidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: 0.024 mM
0.0097
1,1'-(benzene-1,4-diyldimethanediyl)bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
Homo sapiens
-
IC50: 0.0097 mM
0.0029
1,1'-(benzene-1,4-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]pyridinium) dibromide
Homo sapiens
-
IC50: 0.0029 mM
0.015
1,1'-(benzene-1,4-diyldimethanediyl)bis[4-[(phenylamino)methyl]pyridinium] dibromide
Homo sapiens
-
IC50: 0.015 mM
0.0119
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-amino-3-methylquinolinium) dibromide
Homo sapiens
-
IC50: 0.0119 mM
0.0206
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-amino-7-chloroquinolinium) dibromide
Homo sapiens
-
IC50: 0.0206 mM
0.0012
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-aminoquinolinium) dibromide
Homo sapiens
-
IC50: 0.0012 mM
0.0005
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-azepan-1-ylquinolinium) dibromide
Homo sapiens
-
IC50: 0.0005 mM
0.0019
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-piperidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: 0.0019 mM
0.0058
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-pyrrolidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: 0.0058 mM
0.0026
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
Homo sapiens
-
IC50: 0.0026 mM
0.0015
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]pyridinium) dibromide
Homo sapiens
-
IC50: 0.0015 mM
0.0021
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
Homo sapiens
-
IC50: 0.0021 mM
0.147
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(7-amino-4-[[(4-chlorophenyl)amino]methyl]-8-methylquinolinium) dibromide
Homo sapiens
-
IC50: 0.147 mM
0.0012
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(7-chloro-4-pyrrolidin-1-ylquinolinium) dibromide
Homo sapiens
-
IC50: 0.0012 mM
0.0057
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis(7-chloro-4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
Homo sapiens
-
IC50: 0.0057 mM
0.0044
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[4-(dimethylamino)quinolinium] dibromide
Homo sapiens
-
IC50: 0.0044 mM
0.0013
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[4-(phenylamino)quinolinium] dibromide
Homo sapiens
-
IC50: 0.0013 mM
0.00043
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[4-[(phenylamino)methyl]pyridinium] dibromide
Homo sapiens
-
IC50: 0.00043 mM
0.0004
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[4-[(phenylamino)methyl]quinolinium] dibromide
Homo sapiens
-
IC50: 0.0004 mM
0.0568
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[7-amino-8-methyl-4-[(phenylamino)methyl]quinolinium] dibromide
Homo sapiens
-
IC50: 0.0568 mM
0.0096
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[7-chloro-4-(dimethylamino)quinolinium] dibromide
Homo sapiens
-
IC50: 0.0096 mM
0.0031
1,1'-(biphenyl-3,3'-diyldimethanediyl)bis[7-chloro-4-[(phenylamino)methyl]quinolinium] dibromide
Homo sapiens
-
IC50: 0.0031 mM
0.2
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-amino-3-methylquinolinium) dibromide
Homo sapiens
-
IC50: above 0.2 mM
0.0633
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-amino-7-chloroquinolinium) dibromide
Homo sapiens
-
IC50: 0.0633 mM
0.0811
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-aminoquinolinium) dibromide
Homo sapiens
-
IC50: 0.0811 mM
0.0022
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-azepan-1-ylquinolinium) dibromide
Homo sapiens
-
IC50: 0.0022 mM
0.0088
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-piperidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: 0.0088 mM
0.0111
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-pyrrolidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: 0.0111 mM
0.0034
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
Homo sapiens
-
IC50: 0.0034 mM
0.0018
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]pyridinium) dibromide
Homo sapiens
-
IC50: 0.0018 mM
0.002
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
Homo sapiens
-
IC50: 0.002 mM
0.0461
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(7-amino-4-[[(4-chlorophenyl)amino]methyl]-8-methylquinolinium) dibromide
Homo sapiens
-
IC50: 0.0461 mM
0.0198
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(7-chloro-4-pyrrolidin-1-ylquinolinium) dibromide
Homo sapiens
-
IC50: 0.0198 mM
0.0114
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis(7-chloro-4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
Homo sapiens
-
IC50: 0.0114 mM
0.0397
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[4-(dimethylamino)quinolinium] dibromide
Homo sapiens
-
IC50: 0.0397 mM
0.0178
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[4-(phenylamino)quinolinium] dibromide
Homo sapiens
-
IC50: 0.0178 mM
0.0043
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[4-[(phenylamino)methyl]pyridinium] dibromide
Homo sapiens
-
IC50: 0.0043 mM
0.003
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[4-[(phenylamino)methyl]quinolinium] dibromide
Homo sapiens
-
IC50: 0.003 mM
0.0961
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[7-amino-8-methyl-4-[(phenylamino)methyl]quinolinium] dibromide
Homo sapiens
-
IC50: 0.0961 mM
0.0206
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[7-chloro-4-(dimethylamino)quinolinium] dibromide
Homo sapiens
-
IC50: 0.0206 mM
0.0114
1,1'-(biphenyl-4,4'-diyldimethanediyl)bis[7-chloro-4-[(phenylamino)methyl]quinolinium] dibromide
Homo sapiens
-
IC50: 0.0114 mM
0.08
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis(4-aminoquinolinium)
Homo sapiens
-
-
0.0006
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis(4-azepan-1-ylquinolinium)
Homo sapiens
-
-
0.0102
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[4-(dimethylamino)quinolinium]
Homo sapiens
-
-
0.0023
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[4-(phenylamino)quinolinium]
Homo sapiens
-
-
0.0048
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[4-[(4-chlorophenyl)(methyl)amino]quinolinium]
Homo sapiens
-
-
0.0014
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[4-[methyl(phenyl)amino]quinolinium]
Homo sapiens
-
-
0.0575
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-amino-4-[(4-chlorophenyl)(methyl)amino]-8-methylquinolinium]
Homo sapiens
-
-
0.133
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-amino-8-methyl-4-[methyl(phenyl)amino]quinolinium]
Homo sapiens
-
-
0.009
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-chloro-4-(dimethylamino)quinolinium]
Homo sapiens
-
-
0.0057
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-chloro-4-[(4-chlorophenyl)(methyl)amino]quinolinium]
Homo sapiens
-
-
0.0035
1,1'-(ethane-1,2-diyldibenzene-4,1-diyl)bis[7-chloro-4-[methyl(phenyl)amino]quinolinium]
Homo sapiens
-
-
0.01109
1,1'-biphenyl-3,3'-diylbis(4-amino-3-methylquinolinium)
Homo sapiens
-
-
0.0206
1,1'-biphenyl-3,3'-diylbis(4-amino-7-chloroquinolinium)
Homo sapiens
-
-
0.0012
1,1'-biphenyl-3,3'-diylbis(4-aminoquinolinium)
Homo sapiens
-
-
0.0005
1,1'-biphenyl-3,3'-diylbis(4-azepan-1-ylquinolinium)
Homo sapiens
-
-
0.0012
1,1'-biphenyl-3,3'-diylbis(7-chloro-4-pyrrolidin-1-ylquinolinium)
Homo sapiens
-
-
0.0044
1,1'-biphenyl-3,3'-diylbis[4-(dimethylamino)quinolinium]
Homo sapiens
-
-
0.0013
1,1'-biphenyl-3,3'-diylbis[4-(phenylamino)quinolinium]
Homo sapiens
-
-
0.0021
1,1'-biphenyl-3,3'-diylbis[4-[(4-chlorophenyl)(methyl)amino]quinolinium]
Homo sapiens
-
-
0.0004
1,1'-biphenyl-3,3'-diylbis[4-[methyl(phenyl)amino]quinolinium]
Homo sapiens
-
-
0.147
1,1'-biphenyl-3,3'-diylbis[7-amino-4-[(4-chlorophenyl)(methyl)amino]-8-methylquinolinium]
Homo sapiens
-
-
0.0568
1,1'-biphenyl-3,3'-diylbis[7-amino-8-methyl-4-[methyl(phenyl)amino]quinolinium]
Homo sapiens
-
-
0.0096
1,1'-biphenyl-3,3'-diylbis[7-chloro-4-(dimethylamino)quinolinium]
Homo sapiens
-
-
0.0057
1,1'-biphenyl-3,3'-diylbis[7-chloro-4-[(4-chlorophenyl)(methyl)amino]quinolinium]
Homo sapiens
-
-
0.0031
1,1'-biphenyl-3,3'-diylbis[7-chloro-4-[methyl(phenyl)amino]quinolinium]
Homo sapiens
-
-
0.0633
1,1'-biphenyl-4,4'-diylbis(4-amino-7-chloroquinolinium)
Homo sapiens
-
-
0.0811
1,1'-biphenyl-4,4'-diylbis(4-aminoquinolinium)
Homo sapiens
-
-
0.0022
1,1'-biphenyl-4,4'-diylbis(4-azepan-1-ylquinolinium)
Homo sapiens
-
-
0.0198
1,1'-biphenyl-4,4'-diylbis(7-chloro-4-pyrrolidin-1-ylquinolinium)
Homo sapiens
-
-
0.0397
1,1'-biphenyl-4,4'-diylbis[4-(dimethylamino)quinolinium]
Homo sapiens
-
-
0.0178
1,1'-biphenyl-4,4'-diylbis[4-(phenylamino)quinolinium]
Homo sapiens
-
-
0.002
1,1'-biphenyl-4,4'-diylbis[4-[(4-chlorophenyl)(methyl)amino]quinolinium]
Homo sapiens
-
-
0.003
1,1'-biphenyl-4,4'-diylbis[4-[methyl(phenyl)amino]quinolinium]
Homo sapiens
-
-
0.0461
1,1'-biphenyl-4,4'-diylbis[7-amino-4-[(4-chlorophenyl)(methyl)amino]-8-methylquinolinium]
Homo sapiens
-
-
0.0961
1,1'-biphenyl-4,4'-diylbis[7-amino-8-methyl-4-[methyl(phenyl)amino]quinolinium]
Homo sapiens
-
-
0.0206
1,1'-biphenyl-4,4'-diylbis[7-chloro-4-(dimethylamino)quinolinium]
Homo sapiens
-
-
0.0114
1,1'-biphenyl-4,4'-diylbis[7-chloro-4-[(4-chlorophenyl)(methyl)amino]quinolinium]
0.098
1,1'-[(1S,2S)-cyclopropane-1,2-diyldiethane-2,1-diyl]bis(4-piperidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: 0.098 mM
0.1
1,1'-[(1S,2S)-cyclopropane-1,2-diyldiethane-2,1-diyl]bis(4-pyrrolidin-1-ylpyridinium) dibromide
Homo sapiens
-
IC50: above 0.1 mM
0.0482 - 0.1
1,1'-[(1S,2S)-cyclopropane-1,2-diyldiethane-2,1-diyl]bis(4-[[(3,5-dichlorophenyl)amino]methyl]pyridinium) dibromide
0.1
1,1'-[(1S,2S)-cyclopropane-1,2-diyldiethane-2,1-diyl]bis[4-[(phenylamino)methyl]pyridinium] dibromide
Homo sapiens
-
IC50: above 0.1 mM
0.2
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(4-amino-7-chloroquinolinium) dibromide
Homo sapiens
-
IC50: above 0.2 mM
0.08
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(4-aminoquinolinium) dibromide
Homo sapiens
-
IC50: 0.08 mM
0.006
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(4-azepan-1-ylquinolinium) dibromide
Homo sapiens
-
IC50: 0.006 mM
0.0048
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
Homo sapiens
-
IC50: 0.0048 mM
0.0575
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(7-amino-4-[[(4-chlorophenyl)amino]methyl]-8-methylquinolinium) dibromide
Homo sapiens
-
IC50: 0.0575 mM
0.001
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(7-chloro-4-pyrrolidin-1-ylquinolinium) dibromide
Homo sapiens
-
IC50: 0.001 mM
0.0057
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis(7-chloro-4-[[(4-chlorophenyl)amino]methyl]quinolinium) dibromide
Homo sapiens
-
IC50: 0.0057 mM
0.0102
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[4-(dimethylamino)quinolinium] dibromide
Homo sapiens
-
IC50: 0.0102 mM
0.0023
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[4-(phenylamino)quinolinium] dibromide
Homo sapiens
-
IC50: 0.0023 mM
0.0014
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[4-[(phenylamino)methyl]quinolinium] dibromide
Homo sapiens
-
IC50: 0.0014 mM
0.133
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[7-amino-8-methyl-4-[(phenylamino)methyl]quinolinium] dibromide
Homo sapiens
-
IC50: 0.133 mM
0.009
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[7-chloro-4-(dimethylamino)quinolinium] dibromide
Homo sapiens
-
IC50: 0.009 mM
0.0035
1,1'-[ethane-1,2-diylbis(benzene-4,1-diylmethanediyl)]bis[7-chloro-4-[(phenylamino)methyl]quinolinium] dibromide
Homo sapiens
-
IC50: 0.0035 mM
0.00621
1-(4-[4-[(6-amino-3H-purin-3-yl)methyl]phenyl]benzyl)-4-(dimethylamino)pyridinium bromide
Homo sapiens
P35790
in 100 mM Tris (pH 8.5), 10 mM MgCl2, at 37C
0.0389
1-[4-(4-[4-[(6-amino-3H-purin-3-yl)methyl]phenyl]butyl)benzyl]-4-(dimethylamino)pyridinium bromide
Homo sapiens
P35790
in 100 mM Tris (pH 8.5), 10 mM MgCl2, at 37C
0.0107
1-[4-(4-[4-[(6-amino-9H-purin-9-yl)methyl]phenyl]butyl)benzyl]-4-(dimethylamino)pyridinium bromide
Homo sapiens
P35790
in 100 mM Tris (pH 8.5), 10 mM MgCl2, at 37C
0.0132
12,19-diaza-1,8-diazoniapentacyclo[18.2.2.23,6.28,11.214,17]triaconta-1(22),3,5,8,10,14,16,20,23,25,27,29-dodecaene dibromide
Homo sapiens
-
IC50: 0.0132 mM
0.0021
12,21-diaza-1,8-diazoniapentacyclo[20.2.2.23,6.28,11.014,19]triaconta-1(24),3,5,8,10,14,16,18,22,25,27,29-dodecaene dibromide
Homo sapiens
-
IC50: 0.0021 mM
0.0003
14,21-diaza-1,10-diazoniapentacyclo[20.2.2.210,13.216,19.03,8]triaconta-1(24),3,5,7,10,12,16,18,22,25,27,29-dodecaene dibromide
3.2
2-amino-1-butanol
Plasmodium falciparum 3D7
Q8IM71
endogenous enzyme
0.0014
4-chloro-N-methylanilino
Homo sapiens
-
IC50: 0.0014 mM
0.0248
5,10,11,16,17,22-hexahydro-6,9:18,21-diethenodibenzo[g,p][1,5,10,14]tetraazacyclooctadecine-6,21-diium dibromide
Homo sapiens
-
IC50: 0.0248 mM
0.001
7-chloro-1-(4-[2-[4-(7-chloro-5-pyrrolidin-1-ylquinolinium-1-yl)phenyl]ethyl]phenyl)-4-pyrrolidin-1-ylquinolinium
Homo sapiens
-
-
0.005 - 0.1075
choline kinase inhibitor Mn58b
0.057 - 0.58
hemicholinium-3
0.0001 - 0.072
MN58b
0.52 - 1.12
SAR97276
0.00047
V-11-023907
Homo sapiens
-
in 100 mM Tris-HCl pH 7.5, 100 mM KCl, 10 mM MgCl2, at 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000139
-
skeletal muscle, hindlimbs of +/+ CK-alpha mice, supernatant of tissue homogenates are incubated in volume of 0.100 ml of reaction buffer at 37C for 30 min. The reaction product, phosphocholine, is separated using an AG1-X8 column.To determine the activity of each CK isoform, supernatant fractions are treated with an antisera raised against GST (control), GST-CK-alpha or GST-CK-beta fusion proteins combined with protein A-Sepharose overnight at 4C, and the supernatant is used.
0.000484
-
in 40 mM Tris/HCl with 10 mM MgCl2, 2 mM ATP and 0.25 mM choline, pH 7.4, at 37C
0.0045
-
pH 9.0
2.4
-
isozyme CKB-2, pH 10.0, 37C
10.67
-
2 microgram of purified protein is incubated with a reaction mixture of 100 mM MOPS (pH 7.8), 6 mM MgCl2 and 5 mM ATP, with either 2 mM ethanolamine and 200 nanoCi of [3H]ethanolamine or 2 mM choline and 200 nanoCi of (3H)choline at 37C for 20 min and quenched by boiling at 100C for 5 min. Substrates and products are separated by high-performance TLC using silica 60 plates with methanol/0.6% NaCl/ammonium hydroxide as solvent. Radiolabelled species are detected by fluorography at -70C.
10.8
recombinant protein
43
-
isozyme CKA-2, pH 10.0, 37C
143
-
pH 8.5, 37C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
ADP + O-phosphocholine
7.4
-
assay at
7.8
-
The pH optimum is assessed by measuring choline kinase activities at various pH values from pH 5.0 to pH 9.0
8
-
2 optima: pH 8.0 and pH 9.0, cytosolic enzyme
8 - 9.5
-
-
8
-
assay at
9
-
2 optima: pH 8.0 and pH 9.0, cytosolic enzyme
9.2
-
-
9.2 - 9.6
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9
-
pH 7.5: about 30% of maximum activity, pH 9.0: about 45% of maximum activity
7.5 - 10
-
-
8.5 - 10.5
-
about 80% of maximum activity at pH 8.5 and pH 10.5
10.5
-
increase of activity up to
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.1
-
isoelectric disc gel electrophoresis, isoenzyme C
4.7 - 4.8
4.8
-
isoelectric focusing, isoform CKI
5
-
isoelectric disc gel electrophoresis, isoenzyme B
10
-
isoelectric focusing, basic isoform
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
IBEC (CC-2541), primary bronchial epithelial cell
Manually annotated by BRENDA team
-
CC-2551, primary mammary epithelial cell
Manually annotated by BRENDA team
-
lactating
Manually annotated by BRENDA team
-
of the intestine
Manually annotated by BRENDA team
-
non-small cell lung cancer epithelial cell line
Manually annotated by BRENDA team
-
non-small cell lung cancer epithelial cell line
Manually annotated by BRENDA team
-
small cell lung cancer cell line
Manually annotated by BRENDA team
-
small cell lung cancer cell line
Manually annotated by BRENDA team
-
hypocotyl hooks
Manually annotated by BRENDA team
-
strains W303-1A,DY1457, ZHY6, ZHY3, SH304, SH303, SH307
Manually annotated by BRENDA team
additional information
-
parasites isolated from infected human erythrocytes
Manually annotated by BRENDA team