Information on EC 2.7.1.28 - triokinase

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The expected taxonomic range for this enzyme is: Rodentia

EC NUMBER
COMMENTARY
2.7.1.28
-
RECOMMENDED NAME
GeneOntology No.
triokinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
-
sucrose degradation V (sucrose alpha-glucosidase)
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-glyceraldehyde 3-phosphotransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-triokinase
-
-
-
-
kinase, trio (phosphorylating)
-
-
-
-
triose kinase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-65-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
caloric restricted young (3 months) and old (30 months) male C57BL/6J mice
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-glyceraldehyde
ADP + D-glyceraldehyde-3-phosphate
show the reaction diagram
-
-
-
?
ATP + D-glyceraldehyde
ADP + D-glyceraldehyde-3-phosphate
show the reaction diagram
-
-
-
-
-
ATP + D-glyceraldehyde
ADP + D-glyceraldehyde-3-phosphate
show the reaction diagram
-
enzyme of Hers' pathway for fructose metabolism, main pathway for D-glyceraldehyde metabolism in liver
-
?
ATP + dihydroxyacetone
ADP + dihydroxyacetone phosphate
show the reaction diagram
-
-
-
?
ATP + dihydroxyacetone
ADP + dihydroxyacetone phosphate
show the reaction diagram
-
2.5fold higher activity than with D-glyceraldehyde
-
?
ATP + glyceraldehyde
ADP + D-glyceraldehyde-3-phosphate
show the reaction diagram
-
-
-
-
-
ATP + glyceraldehyde
ADP + D-glyceraldehyde-3-phosphate
show the reaction diagram
-
D-glyceraldehyde and DL-glyceraldehyde
-
?
GTP + D-glyceraldehyde
GDP + D-glyceraldehyde-3-phosphate
show the reaction diagram
-
10% of the activity with ATP
-
?
ITP + D-glyceraldehyde
IDP + D-glyceraldehyde-3-phosphate
show the reaction diagram
-
14% of the activity with ATP
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-glyceraldehyde
ADP + D-glyceraldehyde-3-phosphate
show the reaction diagram
-
-
-
?
ATP + D-glyceraldehyde
ADP + D-glyceraldehyde-3-phosphate
show the reaction diagram
-
enzyme of Hers' pathway for fructose metabolism, main pathway for D-glyceraldehyde metabolism in liver
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
required in equimolar concentration with ATP
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ADP
-
free ADP, 0.5 mM, 50% inhibition, competitive to MgATP2-, noncompetitive to D-glyceraldehyde
ATP
-
free ATP, 1.5 mM, 50% inhibition, competitive to MgATP2-, noncompetitive to D-glyceraldehyde
MgADP-
-
free, competitive to MgATP2-, noncompetitive to D-glyceraldehyde
additional information
-
not inhibited by Zn2+
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.008
-
D-glyceraldehyde
-
pH 7.5, 23-24C, cosubstrate 1.0 mM MgATP2-
0.012
-
D-glyceraldehyde
-
pH 7.5, 23-24C, cosubstrate 0.5 mM MgATP2-
0.019
-
D-glyceraldehyde
-
pH 7.5, 23-24C, cosubstrate 2.0 mM MgATP2-
0.006
-
Dihydroxyacetone
-
pH 7.5, 23-24C, at 2 mM ATPMg2-
0.01
-
glyceraldehyde
-
pH 7.0, 22C
0.16
-
MgATP2-
-
pH 7.5, 23-24C, 11.2 mM free Mg2+
0.2
-
MgATP2-
-
pH 7.5, 23-24C, 1.11 mM free Mg2+
0.24
-
MgATP2-
-
pH 7.5, 23-24C, 0.34 mM free Mg2+
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.1
-
ADP
-
pH 7.5, 23-24C
0.38
-
ATP
-
pH 7.5, 23-24C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0024
-
-
activity in the liver of female rats fed with fructose diet containing copper-adequate
0.0034
-
-
activity in the liver of male rats fed with fructose diet containing copper-adequate
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
in young caloric restricted mice the triokinase activity is unchanged compared with control mice. In old caloric restricted mice the triokinase activity is increased about 16% compared to the control group. Comparison between young and old caloric restricted mice, the old have a significantly increased triokinase activity in hepatic tissue (enzyme assay is perfomed at 25C).
Manually annotated by BRENDA team
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partially purified
-