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Information on EC 2.7.1.23 - NAD+ kinase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9C5W3
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2.7.1.23 NAD+ kinaseSpecify your search results
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
nad kinase, nadk, nad+ kinase, nadk2, nad(h) kinase, utr1p, osnadk1, slr0400, c5orf33, sll1415, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ATP:NAD 2'-phosphotransferase
-
-
-
-
DPN kinase
-
-
-
-
kinase (phosphorylating), nicotinamide adenine dinucleotide
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-
-
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kinase, nicotinamide adenine dinucleotide (phosphorylating)
-
-
-
-
NAD kinase
-
-
-
-
NADK
-
-
-
-
NADK1
NADK2
nicotinamide adenine dinucleotide kinase
-
-
-
-
Poly(P)/ATP NAD kinase
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-
-
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polyphosphate/ATP-NAD kinase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:NAD+ 2'-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY
9032-66-0
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
NADP is not phosphorylated, tetrapolyphosphate or diphosphate are not used as phosphoryl donors
-
-
?
additional information
?
-
-
does not accept poly(P) as phosphoryl donor
-
-
?
additional information
?
-
does not accept poly(P) as phosphoryl donor
-
-
?
additional information
?
-
does not accept poly(P) as phosphoryl donor
-
-
?
additional information
?
-
does not accept poly(P) as phosphoryl donor
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
additional information
additional information
-
NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
-
NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
-
NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
additional information
NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Trifluoperazine
-
calmodulin-dependent isoform, 50% inhibition at 0.057 mM, isoforms NADK1, NADK2, no effect
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
H2O2
upregulates NADK1 by 3fold after treatment of plant cells with 5 mM
Calmodulin
-
calmodulin-dependent isoform, full activation in presence of Ca2+
additional information
-
NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin, although recombinant NADK2, which has a calmodulin-binding motif in its N-terminal, is able to bind to calmodulin
-
additional information
NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin, although recombinant NADK2, which has a calmodulin-binding motif in its N-terminal, is able to bind to calmodulin
-
additional information
NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin, although recombinant NADK2, which has a calmodulin-binding motif in its N-terminal, is able to bind to calmodulin
-
additional information
NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin, although recombinant NADK2, which has a calmodulin-binding motif in its N-terminal, is able to bind to calmodulin
-
additional information
-
irradiation upregulates NADK1 by 8fold. NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
-
additional information
irradiation upregulates NADK1 by 8fold. NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
-
additional information
irradiation upregulates NADK1 by 8fold. NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
-
additional information
irradiation upregulates NADK1 by 8fold. NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
-
additional information
-
NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
-
additional information
NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
-
additional information
NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
-
additional information
NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.74
ATP
pH and temperature not specified in the publication
0.43
NAD+
pH and temperature not specified in the publication
0.042
NADH
pH and temperature not specified in the publication
0.062
ATP
pH and temperature not specified in the publication
0.73
ATP
pH and temperature not specified in the publication
0.52
NAD+
pH and temperature not specified in the publication
2.39
NAD+
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
11.1
14.3
213
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.9
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
-
both isoforms NADK1 and NADK2 are present in all tissues examined and throughout development
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
chloroplast NAD kinase 2 has an important role in chlorophyll biosynthesis and protects chloroplasts from oxidative stress
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NADK2_ARATH
985
0
109188
Swiss-Prot
Chloroplast (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
58200
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
Ca2+-dependent calmodulin-binding domain near N-terminal extension of isoform NADK2
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
NADK2-deficient mutant grows slowly, is sensitive to environmental stress, which leads to oxidative stress, and shows reduced chlorophyll content. NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress
additional information
NADK2-deficient mutant grows slowly, is sensitive to environmental stress, which leads to oxidative stress, and shows reduced chlorophyll content. NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress
additional information
NADK2-deficient mutant grows slowly, is sensitive to environmental stress, which leads to oxidative stress, and shows reduced chlorophyll content. NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress
additional information
NADK2-deficient mutant grows slowly, is sensitive to environmental stress, which leads to oxidative stress, and shows reduced chlorophyll content. NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress
additional information
-
enzyme knockout mutant, growth inhibition and smaller rosette leaves than wild-type, with pale yellow colour. Mutant plant show a reduced chlorophyll content, and hypersensitivity to environmental stress such as UV-B, drought, heat shock and salinity
additional information
-
NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress
additional information
NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress
additional information
NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress
additional information
NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant isoforms NADK1, NADK2, partial purification of calmodulin-dependent isoform
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
low upregulation by salt stress, moderate upregulatation by heat stress
high upregulation by drought strss and salt stress, low upregulatation by heat stress and cold stress
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
studies of enzyme activation by calmodulin, calmodulin-like proteins, calcium-binding proteins, and calmodulin mutants
additional information
additional information
-
one of the key enzymes regulating the balance of NAD(H) and NADP(H)
additional information
one of the key enzymes regulating the balance of NAD(H) and NADP(H)
additional information
one of the key enzymes regulating the balance of NAD(H) and NADP(H)
additional information
one of the key enzymes regulating the balance of NAD(H) and NADP(H)
additional information
-
one of the key enzymes regulating the balance of NAD(H) and NADP(H)
additional information
one of the key enzymes regulating the balance of NAD(H) and NADP(H)
additional information
one of the key enzymes regulating the balance of NAD(H) and NADP(H)
additional information
one of the key enzymes regulating the balance of NAD(H) and NADP(H)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Turner, W.L.; Waller, J.C.; Vanderbeld, B.; Snedden, W.A.
Cloning and characterization of two NAD kinases from Arabidopsis. identification of a calmodulin binding isoform
Plant Physiol.
135
1243-1255
2004
Arabidopsis thaliana
Chai, M.F.; Chen, Q.J.; An, R.; Chen, Y.M.; Chen, J.; Wang, X.C.
NADK2, an Arabidopsis chloroplastic NAD kinase, plays a vital role in both chlorophyll synthesis and chloroplast protection
Plant Mol. Biol.
59
553-564
2005
Arabidopsis thaliana
Kawai, S.; Murata, K.
Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H)
Biosci. Biotechnol. Biochem.
72
919-930
2008
Arabidopsis thaliana, Arabidopsis thaliana (Q500Y9), Arabidopsis thaliana (Q56YN3), Arabidopsis thaliana (Q9C5W3), Archaeoglobus fulgidus, Bacillus subtilis (O31612), Bacillus subtilis (O34934), Candida albicans, Columba livia, Cyberlindnera jadinii, Dictyostelium discoideum, Escherichia coli (P0A7B3), Glycine max, Homo sapiens, Homo sapiens (O95544), Lactuca sativa, Listeria monocytogenes, Methanocaldococcus jannaschii (Q58327), Micrococcus luteus (Q8VUL9), Mycobacterium tuberculosis (P9WHV7), Mycobacterium tuberculosis H37Rv (P9WHV7), Oryza sativa, Pyrococcus horikoshii (O58801), Saccharomyces cerevisiae (C7GKE4), Saccharomyces cerevisiae (P21373), Saccharomyces cerevisiae (P32622), Saccharomyces cerevisiae (Q06892), Saccharomyces cerevisiae, Salmonella enterica, Schizosaccharomyces pombe, Sorghum sp., Sphingomonas sp. A1 (Q6L7J5), Synechococcus elongatus PCC 6301, Thermotoga maritima, Triticum sp.
Takahashi, H.; Takahara, K.; Hashida, S.N.; Hirabayashi, T.; Fujimori, T.; Kawai-Yamada, M.; Yamaya, T.; Yanagisawa, S.; Uchimiya, H.
Pleiotropic modulation of carbon and nitrogen metabolism in Arabidopsis plants overexpressing the NAD kinase2 gene
Plant Physiol.
151
100-113
2009
Arabidopsis thaliana
Waller, J.C.; Dhanoa, P.K.; Schumann, U.; Mullen, R.T.; Snedden, W.A.
Subcellular and tissue localization of NAD kinases from Arabidopsis: compartmentalization of de novo NADP biosynthesis
Planta
231
305-317
2010
Arabidopsis thaliana
Li, B.; Wang, X.; Tai, L.; Ma, T.; Shalmani, A.; Liu, W.; Li, W.; Chen, K.
NAD kinases Metabolic targets controlling redox co-enzymes and reducing power partitioning in plant stress and development
Front. Plant Sci.
9
379
2018
Arabidopsis thaliana (Q500Y9), Arabidopsis thaliana (Q56YN3), Arabidopsis thaliana (Q9C5W3), Archaeoglobus fulgidus (P0A7B3), Bacillus subtilis (M4KX93), Escherichia coli (P0A7B3), Escherichia coli K12 (P0A7B3), Glycine max (I1KAE3), Glycine max (I1LPA7), Glycine max (I1LVA5), Glycine max (K7LNT1), Glycine max (K7LY64), Homo sapiens (O95544), Homo sapiens (Q4G0N4), Pyrococcus horikoshii (O58801), Pyrococcus horikoshii ATCC 700860 (O58801), Solanum lycopersicum (A0A3Q7E928), Solanum lycopersicum (A0A3Q7JHB9), Sphingomonas sp. (O58801), Strongylocentrotus purpuratus (C3RSF7), Strongylocentrotus purpuratus (P21373)
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