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Information on EC 2.7.1.21 - thymidine kinase and Organism(s) Dictyostelium discoideum and UniProt Accession Q27564

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EC Tree
IUBMB Comments
Deoxyuridine can also act as acceptor, and dGTP can act as a donor. The deoxypyrimidine kinase complex induced by Herpes simplex virus catalyses this reaction as well as those of EC 2.7.1.114 (AMP---thymidine kinase), EC 2.7.1.118 (ADP---thymidine kinase) and EC 2.7.4.9 (dTMP-kinase).
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This record set is specific for:
Dictyostelium discoideum
UNIPROT: Q27564
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The taxonomic range for the selected organisms is: Dictyostelium discoideum
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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ATP
+
thymidine
=
ADP
+
dTMP
+
=
+
Synonyms
thymidine kinase, hsv1-tk, calmodulin-binding protein, thymidine kinase 1, thymidylate kinase, type 1 thymidine kinase, hsv-1 tk, thymidine kinase 2, mitochondrial thymidine kinase, cam-binding protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
calmodulin-binding protein
-
CaM-binding protein
-
2'-deoxythymidine kinase
-
-
-
-
ATP-thymidine 5'-phosphotransferase
-
-
-
-
EC 2.7.1.75
-
-
-
-
kinase, deoxythymidine (phosphorylating)
-
-
-
-
kinase, thymidine (phosphorylating)
-
-
-
-
TdR kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:thymidine 5'-phosphotransferase
Deoxyuridine can also act as acceptor, and dGTP can act as a donor. The deoxypyrimidine kinase complex induced by Herpes simplex virus catalyses this reaction as well as those of EC 2.7.1.114 (AMP---thymidine kinase), EC 2.7.1.118 (ADP---thymidine kinase) and EC 2.7.4.9 (dTMP-kinase).
CAS REGISTRY NUMBER
COMMENTARY hide
9002-06-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + thymidine
ADP + dTMP
show the reaction diagram
-
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + thymidine
ADP + thymidine 5'-phosphate
show the reaction diagram
-
-
-
ir
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
P-loop binding site
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
calmodulin binding binding is dependent on Ca2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
W7
slight inhibitory in presence of calmodulin
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Calmodulin
stimulates the enzyme by 13.8%, might modify the activity, enzyme contains a calmodulin binding domain, Ca2+ is required for binding, compounds W7 and W13 prevent stimulation, overview
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0024 - 0.008
thymidine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KITH_DICDI
227
0
25494
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25400
2 * 25400, calculated from amino acid sequence
25494
x * 25494, recombinant enzyme, amino acid sequence calculation
57000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 25494, recombinant enzyme, amino acid sequence calculation
homodimer
2 * 25400, calculated from amino acid sequence
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme partially utilizing calmodulin-agarose-beads
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
gene thyB, DNA and amino acid sequence determination and analysis, phage display, location on chromosome 5, 3 exons, 2 introns, expression in Escherichia coli strain BL21
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
O'Day, D.H.; Chatterjee-Chakraborty, M.; Wagler, S.; Myre, M.A.
Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein
Biochem. Biophys. Res. Commun.
331
1494-1502
2005
Dictyostelium discoideum (Q27564), Dictyostelium discoideum
Manually annotated by BRENDA team
Mutahir, Z.; Clausen, A.R.; Andersson, K.M.; Wisen, S.M.; Munch-Petersen, B.; Piskur, J.
Thymidine kinase 1 regulatory fine-tuning through tetramer formation
FEBS J.
280
1531-1541
2013
Anolis carolinensis (C3VAL3), Caenorhabditis elegans (G5EEZ5), Gallus gallus (P04047), Homo sapiens (P04183), Homo sapiens, Rattus norvegicus (P27158), Bacillus cereus (Q0H0H6), Dictyostelium discoideum (Q27564), Xenopus tropicalis (Q5I0A2), Danio rerio (Q803Y1), Arabidopsis thaliana (Q9S750 and Q9FN47)
Manually annotated by BRENDA team