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Information on EC 2.7.1.19 - phosphoribulokinase and Organism(s) Spinacia oleracea and UniProt Accession P09559

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This record set is specific for:
Spinacia oleracea
UNIPROT: P09559 not found.
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The taxonomic range for the selected organisms is: Spinacia oleracea
The enzyme appears in selected viruses and cellular organisms
Synonyms
phosphoribulokinase, ribulose-5-phosphate kinase, ribulose 5-phosphate kinase, crprk, atprk, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-phosphoribulose kinase
-
-
-
-
kinase, phosphoribulo- (phosphorylating)
-
-
-
-
phosphopentokinase
-
-
-
-
PKK
-
-
-
-
Prk
-
-
-
-
PRuK
-
-
-
-
ribulose 5-phosphate kinase
-
-
-
-
ribulose phosphate kinase
-
-
-
-
ribulose-5-phosphate kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-ribulose-5-phosphate 1-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9030-60-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-ribulose 5-phosphate
ADP + D-ribulose 1,5-bisphosphate
show the reaction diagram
D-ribulose 5-phosphate + ATP
D-ribulose 1,5-diphosphate + ADP
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no activity with fructose 6-phosphate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-ribulose 5-phosphate
ADP + D-ribulose 1,5-bisphosphate
show the reaction diagram
part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms
-
-
?
D-ribulose 5-phosphate + ATP
D-ribulose 1,5-diphosphate + ADP
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
can partially replace Mg2+
Co2+
-
can partially replace Mg2+
EDTA
-
activation
Mg2+
-
required
Mn2+
-
can partially replace Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DL-glyceraldehyde
-
non competitive, 10 mM
iodoacetate
-
-
p-chloromercuribenzoate
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thioredoxin
reduced form, regulates activity via reversible reduction, probably not relevant in vivo
2-mercaptoethanol
-
above 2 mM
dithiothreitol
-
above 0.5 mM
reduced glutathione
-
above 5 mM
thioredoxin
-
thioredoxinm is more efficient than thioredoxin f
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.625
ATP
-
pH 7.9, 20°C
0.222
D-ribulose 5-phosphate
-
pH 7.9, 20°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
19 - 20
DL-glyceraldehyde
-
pH 7.9, 20°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
410
-
buffer containing fresh dithiothreitol
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.2
-
less than 50% of maximal activity above and below
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KPPR_SPIOL
402
0
45007
Swiss-Prot
Chloroplast (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
-
2 * 44000, SDS-PAGE
48000
-
SDS-PAGE
90000
-
non-denaturing gel electrophoresis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C16S/C244S/C250S
-
triple mutant with altered oxidation-reduction midpoint potential as wild-type enzyme
C244S/C250S
-
double mutant with same oxidation-reduction midpoint potential as wild-type enzyme
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable to repeated freezing/thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, stable
-
-80°C, N2-atmosphere, 50 mM bicine-KOH, 10 mM potassium phosphate, 10 mM dithiothreitol, 1 mM EDTA, 10% v/v glycerol, 2 months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from chloroplasts
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hurwitz, J.; Weissbach, A.; Horecker, B.L.; Smyrniotis, B.L.
Spinach phosphoribulokinase
J. Biol. Chem.
218
769-783
1956
Spinacia oleracea
Manually annotated by BRENDA team
Porter, M.A.; Milanez, S.; Stringer, C.D.; Hartman, F.C.
Purification and characterization of ribulose-5-phosphate kinase from spinach
Arch. Biochem. Biophys.
245
14-23
1986
Spinacia oleracea
Manually annotated by BRENDA team
Slabas, A.R.; Walker, D.A.
Inhibition of spinach phosphoribulokinase by DL-glyceraldehyde
Biochem. J.
153
613-619
1976
Spinacia oleracea
Manually annotated by BRENDA team
Lavergne, D.; Bismuth, E.
Simultaneous purification of two kinases from spinach leaves
Plant Sci. Lett.
1
229-236
1973
Spinacia oleracea
-
Manually annotated by BRENDA team
Krieger, T.J.; Miziorko, H.M.
Spinach leaf ribulose-5-phosphate kinase: examination of sulfhydryls by chemical modification and spin-labeling
Arch. Biochem. Biophys.
256
362-371
1987
Spinacia oleracea
Manually annotated by BRENDA team
Hirasawa, M.; Brandes, H.K.; Hartman, F.C.; Knaff, D.B.
Oxidation-reduction properties of the regulatory site of spinach phosphoribulokinase
Arch. Biochem. Biophys.
350
127-131
1998
Spinacia oleracea
Manually annotated by BRENDA team
Geck, M.K.; Hartman, F.C.
Kinetic and mutational analyses of the regulation of phosphoribulokinase by thioredoxins
J. Biol. Chem.
275
18034-18039
2000
Spinacia oleracea
Manually annotated by BRENDA team
Miziorko, H.M.
Phosphoribulokinase: current perspectives on the structure/function basis for regulation and catalysis
Adv. Enzymol. Relat. Areas Mol. Biol.
74
95-127
2000
Cupriavidus necator, Chlamydomonas reinhardtii, Chlorogloeopsis fritschii, Heterosigma akashiwo, Acidovorax facilis, Nitrobacter winogradskyi, Rhodobacter capsulatus, Cereibacter sphaeroides, Rhodoblastus acidophilus, Spinacia oleracea, Halothiobacillus neapolitanus
Manually annotated by BRENDA team
Michels, A.K.; Wedel, N.; Kroth, P.G.
Diatom plastids possess a phosphoribulokinase with an altered regulation and no oxidative pentose phosphate pathway
Plant Physiol.
137
911-920
2005
Spinacia oleracea (P09559), Spinacia oleracea, Trieres chinensis (Q95033), Trieres chinensis
Manually annotated by BRENDA team