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Information on EC 2.7.1.176 - UDP-N-acetylglucosamine kinase and Organism(s) Streptococcus pyogenes and UniProt Accession Q54944

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IUBMB Comments
Toxic component of a toxin-antitoxin (TA) module. The phosphorylation of UDP-N-acetyl-D-glucosamine results in the inhibition of EC 2.5.1.7, UDP-N-acetylglucosamine 1-carboxyvinyltransferase, the first committed step in cell wall synthesis, which is then blocked. The activity of this enzyme is inhibited when the enzyme binds to the cognate epsilon antitoxin.
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Streptococcus pyogenes
UNIPROT: Q54944
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The taxonomic range for the selected organisms is: Streptococcus pyogenes
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
zeta toxin, zeta-toxin, toxin pezt, toxin zeta, unag kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
toxin PezT
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UNAG kinase
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zeta toxin
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SYSTEMATIC NAME
IUBMB Comments
ATP:UDP-N-acetyl-D-glucosamine 3'-phosphotransferase
Toxic component of a toxin-antitoxin (TA) module. The phosphorylation of UDP-N-acetyl-D-glucosamine results in the inhibition of EC 2.5.1.7, UDP-N-acetylglucosamine 1-carboxyvinyltransferase, the first committed step in cell wall synthesis, which is then blocked. The activity of this enzyme is inhibited when the enzyme binds to the cognate epsilon antitoxin.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + UDP-N-acetyl-D-glucosamine
ADP + UDP-N-acetyl-D-glucosamine 3'-phosphate
show the reaction diagram
additional information
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + UDP-N-acetyl-D-glucosamine
ADP + UDP-N-acetyl-D-glucosamine 3'-phosphate
show the reaction diagram
the kinase activity is responsible for the toxic function in vivo, because the phosphorylated product inhibits MurA, the enzyme responsible for the first step of peptidoglycan synthesis in bacteria, mechanism, overview
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
most genomes of bacteria contain toxin-antitoxin systems. These gene systems encode a toxic protein and its cognate antitoxin. Members of the epsilon/zeta toxin-antitoxin family are found throughout the genomes of pathogenic bacteria
metabolism
mechanism used by zeta toxins to induce programmed cell death in bacteria, overview
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ZTOX_STRPY
287
0
32404
Swiss-Prot
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
analysis of the crystal structure of the epsilon/zeta toxin-antitoxin complex bound to UDP-N-acetyl-D-glucosamine at 2.7 A resolution
purified recombinant wild-type and selenomethionine-derivated epsilon2/zeta2 protein complexes, X-ray diffraction structure determination and analysis at 1.95 A and 3.1 A resolution, respectively, multiple anomalous diffraction, modeling
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A111C
deletion of one A in the AAAAAAA tract, which begins at the 78-nt position, results in a frameshift and the stop codon formation after 51 codons, additional mutations are insertion of G after A32 and A111C substitution in one case and A248G substitution in the second one
A248G
deletion of one A in the AAAAAAA tract, which begins at the 78-nt position, results in a frameshift and the stop codon formation after 51 codons, additional mutations are insertion of G after A32 and A111C substitution in one case and A248G substitution in the second one
D66T
site-directed mutagenesis, a nontoxicPezT variant, no bulge formation or lysis after induction of PezT D66T
D67T
site-directed mutagenesis
K46A
site-directed mutagenesis, the change in the Walker A motif enables cloning and expression of genes K46A and D67T in Escherichia coli without coexpression of the antagonistic gene epsilon
K46A/D67T
site-directed mutagenesis, the change in the Walker A motif enables cloning and expression of genes K46A and D67T in Escherichia coli without coexpression of the antagonistic gene epsilon
R158A
site-directed mutagenesis, the mutant construct can be cloned into Escherichia coli but not overexpressed
R158A/R171S
site-directed mutagenesis, the mutant can be cloned and expressed
R171S
site-directed mutagenesis
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant truncated variant PezTDELTAC242 from Escherichia coli strain BL21(DE3)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence comparisons
gene pezT, DNA and amino acid sequence determination and analysis, expression of truncated variant PezTDELTAC242 in Escherichia coli strain BL21(DE3)
the gene is encoded in plasmid pSM19035. The omega-epsilon-zeta operon of this plasmid constitutes a proteic plasmid addiction system in which the epsilon and zeta genes encode an antitoxin and toxin, respectively, while omega plays an autoregulatory function
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zielenkiewicz, U.; Ceglowski, P.
The toxin-antitoxin system of the streptococcal plasmid pSM19035
J. Bacteriol.
187
6094-6105
2005
Streptococcus pyogenes (Q54944), Streptococcus pyogenes
Manually annotated by BRENDA team
Mutschler, H.; Gebhardt, M.; Shoeman, R.L.; Meinhart, A.
A novel mechanism of programmed cell death in bacteria by toxin-antitoxin systems corrupts peptidoglycan synthesis
PLoS Biol.
9
e1001033
2011
Escherichia coli, Streptococcus pyogenes (Q54944), Streptococcus pneumoniae (Q97QZ1)
Manually annotated by BRENDA team
Meinhart, A.; Alonso, J.C.; Straeter, N.; Saenger, W.
Crystal structure of the plasmid maintenance system epsilon/zeta: functional mechanism of toxin zeta and inactivation by epsilon 2 zeta 2 complex formation
Proc. Natl. Acad. Sci. USA
100
1661-1666
2003
Streptococcus pyogenes (Q54944), Streptococcus pyogenes
Manually annotated by BRENDA team