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ATP + D-xylulose
ADP + D-xylulose 5-phosphate
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-
-
?
ATP + D-ribulose
ADP + D-ribulose 5-phosphate
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60% of the activity with D-xylulose
-
-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
ATP + D-xylulose
?
-
overexpression of xylulokinase in Saccharomyces cerevisiae is detrimental to cell growth on xylulose but not on glucose
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-
?
ATP + D-xylulose
?
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xylulokinase activity is absent during glucose metabolism but is detectable during simultaneous utilization of xylulose and glucose
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-
?
ATP + D-xylulose
?
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expression level of xylulokinase can limit xylulose metabolism, although other factors are also likely to have a role
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-
?
ATP + D-xylulose
?
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the enzyme is involved in one of the initial steps of xylose utilization
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-
?
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
r
ATP + D-xylulose
ADP + D-xylulose 5-phosphate
-
-
-
-
r
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additional information
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0.034 +/-0.002 U/mg, more than 2fold higher values than D-XR/XDH and D-XR/ARSdR, XK reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute.
additional information
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20.6 +/-0.3 Unit/mg at 25°C, approx. 3 mM D-xylulose 5-phosphate could be produced over 10 min at 25°C from 5 mM xylulose with 0.004 mg purified xylulokinase. With 2.5 mM xylulose, about 80% of conversion occur.
additional information
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strain D-XR/ARSdR/XK, 0.030 +/-0.002 U/mg, more than two-fold higher values than D-XR/XDH and D-XR/ARSdR, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute.
additional information
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strain D452-2 D-XR/ARSdR, 0.013 +/-0.001 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute.
additional information
-
strain D452-2 D-XR/XDH, 0.014 +/-0.002 U/mg, reaction forming ADP coupled with pyruvate kinase (PK) and lactate dehydrogenase (LDH) reactions, activity is measured by monitoring the oxidation of NADH at 340 nm in reaction mixture, one unit of enzyme activity is defined as the amount of enzyme that reduces or oxidizes 1 micromol NAD(P)+ or NAD(P)H per minute.
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expression in Escherichia coli BL21(DE3)
expressed in Escherichia coli strain XL1-Blue (Stratagene)
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expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/ARSdR with naturally occuring XK-activity
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expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/ARSdR/XK with upegulated XK-activity, entire coding sequence of XK with PGK promoter and terminator inserted into pAUR101 shuttle vector (Takara Bio, Kyoto) integrating in Saccharomyces cerevisiae strain D452-2
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expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/XDH with naturally occuring enzyme-activity
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expressed in Saccharomyces cerevisiae strain D452-2, DNA from Saccharomyces cerevisiae strain S288C, strain D-XR/XDH/XK with upegulated XK-activity, entire coding sequence of XK with PGK promoter and terminator inserted into pAUR101 shuttle vector integrating in Saccharomyces cerevisiae strain D452-2
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expressed in Saccharomyces cerevisiae under the control of the glyceraldehyde 3-phosphate dehydrogenase promoter and terminator
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overexpression in Saccharomyces cerevisiae
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overexpression of the gene on Saccharomyces cerevisiae H158 and CEN-PK also expressing xylose reductase and xylitol dehydrogenase. Effect on fermentation of xylose and lignocellulosic hydrolysate
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overexpression of xylulokinase in Saccharomyces cerevisiae is detrimental to cell growth on xylulose but not on glucose
-
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Johansson, B.; Christensson, C.; Hobley, T.; Hahn-Hagerdal, B.
Xylulokinase overexpression in two strains of Saccharomyces cerevisiae also expressing xylose reductase and xylitol dehydrogenase and its effect on fermentation of xylose and lignocellulosic hydrolysate
Appl. Environ. Microbiol.
67
4249-4255
2001
Saccharomyces cerevisiae, Saccharomyces cerevisiae CBS 8066
brenda
Richard, P.; Toivari, M.H.; Penttila, M.
The role of xylulokinase in Saccharomyces cerevisiae xylulose catabolism
FEMS Microbiol. Lett.
190
39-43
2000
Saccharomyces cerevisiae, Saccharomyces cerevisiae W303-1B
brenda
Lee, T.H.; Kim, M.D.; Park, Y.C.; Bae, S.M.; Ryu, Y.W.; Seo, J.H.
Effects of xylulokinase activity on ethanol production from D-xylulose by recombinant Saccharomyces cerevisiae
J. Appl. Microbiol.
95
847-852
2003
Saccharomyces cerevisiae
brenda
Jeppsson, H.; Yu, S.; Hahn-Hagerdal, B.
Xylulose and glucose fermentation by Saccharomyces cerevisiae in chemostat culture
Appl. Environ. Microbiol.
62
1705-1709
1996
Saccharomyces cerevisiae
brenda
Wahlbom, C.F.; Cordero Otero, R.R.; van Zyl, W.H.; Hahn-Hagerdal, B.; Jonsson, L.J.
Molecular analysis of a Saccharomyces cerevisiae mutant with improved ability to utilize xylose shows enhanced expression of proteins involved in transport, initial xylose metabolism, and the pentose phosphate pathway
Appl. Environ. Microbiol.
69
740-746
2003
Saccharomyces cerevisiae
brenda
Jin, Y.S.; Ni, H.; Laplaza, J.M.; Jeffries, T.W.
Optimal growth and ethanol production from xylose by recombinant Saccharomyces cerevisiae require moderate D-xylulokinase activity
Appl. Environ. Microbiol.
69
495-503
2003
Saccharomyces cerevisiae, Scheffersomyces stipitis
brenda
Ni, H.; Laplaza, J.M.; Jeffries, T.W.
Transposon mutagenesis to improve the growth of recombinant Saccharomyces cerevisiae on D-xylose
Appl. Environ. Microbiol.
73
2061-2066
2007
Saccharomyces cerevisiae, Saccharomyces cerevisiae L2612
brenda
Lee, J.Y.; Cheong, D.E.; Kim, G.J.
A novel assay system for the measurement of transketolase activity using xylulokinase from Saccharomyces cerevisiae
Biotechnol. Lett.
30
899-904
2008
Saccharomyces cerevisiae
brenda
Matsushika, A.; Watanabe, S.; Kodaki, T.; Makino, K.; Sawayama, S.
Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP(+)-dependent xylitol dehydrogenase, and xylulokinase
J. Biosci. Bioeng.
105
296-299
2008
Saccharomyces cerevisiae, Saccharomyces cerevisiae D452-2 D-XR/XDH/XK, Saccharomyces cerevisiae D452-2 D-XR/ARSdR/XK, Saccharomyces cerevisiae D452-2 D-XR/XDH, Saccharomyces cerevisiae D452-2 D-XR/ARSdR
brenda
Khattab, S.; Kodaki, T.
Efficient bioethanol production by overexpression of endogenous Saccharomyces cerevisiae xylulokinase and NADPH-dependent aldose reductase with mutated strictly NADP+-dependent Pichia stipitis xylitol dehydrogenase
Process Biochem.
49
1838-1842
2014
Saccharomyces cerevisiae
-
brenda
Hardt, N.; Kind, S.; Schoenenberger, B.; Eggert, T.; Obkircher, M.; Wohlgemuth, R.
Facile synthesis of D-xylulose-5-phosphate and L-xylulose-5-phosphate by xylulokinase-catalyzed phosphorylation
Biocatal. Biotransform.
38
35-45
2020
Saccharomyces cerevisiae (P42826), Saccharomyces cerevisiae ATCC 204508 (P42826)
-
brenda