Information on EC 2.7.1.17 - xylulokinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.7.1.17
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RECOMMENDED NAME
GeneOntology No.
xylulokinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + D-xylulose = ADP + D-xylulose 5-phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
Phosphorylation
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Phosphorylation
-
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Phosphorylation
Scheffersomyces stipitis FPL-YS30
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-
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PATHWAY
KEGG Link
MetaCyc Link
D-arabitol degradation
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Metabolic pathways
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Pentose and glucuronate interconversions
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xylitol degradation
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xylose degradation I
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-xylulose 5-phosphotransferase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1-deoxy-D-xylulokinase
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D-xylulokinase
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-
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D-xylulokinase
Q4JHR4
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D-xylulokinase
Q4JHR4
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D-xylulokinase
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D-xylulokinase
O75191
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D-xylulokinase
F6JVF3
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D-xylulokinase
Kluyveromyces marxianus NBRC1777
F6JVF3
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D-xylulokinase
Saccharomyces cerevisiae L2612
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D-xylulose kinase
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kinase (phosphorylating), xylulo
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kinase, xylulo- (phosphorylating)
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XK
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-
-
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XKS1
Saccharomyces cerevisiae L2612
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-
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XYL3
Q4JHR4
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XYL3
Q4JHR4
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XYL3
F6JVF3
gene name
XYL3
Kluyveromyces marxianus NBRC1777
F6JVF3
gene name
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xylokinase(phosphorylating)
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xylulokinase
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xylulokinase
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CAS REGISTRY NUMBER
COMMENTARY
9030-58-4
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
expression in Escherichia coli
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-
Manually annotated by BRENDA team
calf
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-
Manually annotated by BRENDA team
strain Xu316
UniProt
Manually annotated by BRENDA team
strain Xu316
UniProt
Manually annotated by BRENDA team
expressed as constitutive enzyme in Escherichia coli K12 construct strain NC260
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-
Manually annotated by BRENDA team
DELTAxylB mutant strain (PC07) with introduced xyl3 from Pichia stipitis, xylB encodes XK in Escherichia coli
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Manually annotated by BRENDA team
DELTAxylB mutant strain (PC07), xylB encodes XK in Escherichia coli
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Manually annotated by BRENDA team
His-tagged enzyme
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-
Manually annotated by BRENDA team
strain DH5alpha
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-
Manually annotated by BRENDA team
Escherichia coli DH5alpha
strain DH5alpha
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-
Manually annotated by BRENDA team
var. aerogenes 1033, formerly Aerobacter aerogenes 1033, mutant strain KAY2026
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-
Manually annotated by BRENDA team
Kluyveromyces marxianus NBRC1777
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UniProt
Manually annotated by BRENDA team
strain 124-2
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-
Manually annotated by BRENDA team
Lactobacillus pentosus 124-2
strain 124-2
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-
Manually annotated by BRENDA team
endogenous XYL3 gene
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Manually annotated by BRENDA team
strain CBS4732, thermotolerant, recombinant Hansenula polymorpha strain overexpressing the modified XR (K341R N343D) together with native XDH and XK on a DELTAxyl1 background; strain XRm/XDH/XK, overexpression of XK, thermotolerant; strain XRn/XDH/XK, overexpression of XK, thermotolerant
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Manually annotated by BRENDA team
Ogataea angusta XRm/XDH/XK
strain XRm/XDH/XK, overexpression of XK, thermotolerant
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Manually annotated by BRENDA team
Ogataea angusta XRn/XDH/XK
strain XRn/XDH/XK, overexpression of XK, thermotolerant
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Manually annotated by BRENDA team
strain CBS 8066
Uniprot
Manually annotated by BRENDA team
strain D452-2 D-XR/ARSdR; strain D452-2 D-XR/ARSdR/XK; strain D452-2 D-XR/XDH; strain D452-2 D-XR/XDH/XK
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Manually annotated by BRENDA team
strain L2612
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Manually annotated by BRENDA team
strain TMB 3399 and mutant strain TMB 3400 derived from TMB399 and displaying improved ability to utilize xylose with higher expression of xylulokinase
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Manually annotated by BRENDA team
Saccharomyces cerevisiae CBS 8066
strain CBS 8066
Uniprot
Manually annotated by BRENDA team
Saccharomyces cerevisiae D452-2 D-XR/ARSdR
strain D452-2 D-XR/ARSdR
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-
Manually annotated by BRENDA team
Saccharomyces cerevisiae D452-2 D-XR/ARSdR/XK
strain D452-2 D-XR/ARSdR/XK
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-
Manually annotated by BRENDA team
Saccharomyces cerevisiae D452-2 D-XR/XDH
strain D452-2 D-XR/XDH
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Manually annotated by BRENDA team
Saccharomyces cerevisiae D452-2 D-XR/XDH/XK
strain D452-2 D-XR/XDH/XK
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Manually annotated by BRENDA team
Saccharomyces cerevisiae L2612
strain L2612
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Manually annotated by BRENDA team
Saccharomyces cerevisiae W303-1B
W303-1B
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Manually annotated by BRENDA team
strain FPL-YS30
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Manually annotated by BRENDA team
Scheffersomyces stipitis FPL-YS30
strain FPL-YS30
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Manually annotated by BRENDA team
over-expression onf enzyme
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Manually annotated by BRENDA team
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
DTT
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enzyme is inactive in absence either EDTA or a thiol such as reduced monosodium glutathione or dithiothreitol
EDTA
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enzyme is inactive in absence either EDTA or a thiol such as reduced monosodium glutathione or dithiothreitol
Monosodium glutathione
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enzyme is inactive in absence either EDTA or a thiol such as reduced monosodium glutathione or dithiothreitol
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
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assay at
8
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pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
10
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about half-maximal activity at pH 6 and 10
6.7
9
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pH 6.7: about 50% of maximal activity, pH 9.0: about 75% of maximal activity
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
10
40
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steady increase up to 30C, rapid loss above, with 60% of maximal activity at 40C
30
77
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30C: about 50% of maximal activity, 77C: about 65% of maximal activity
PDB
SCOP
CATH
ORGANISM
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Cryptosporidium parvum (strain Iowa II)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255)
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.4
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5 min, 26% loss of activity at 53C, inactivation at 60C
5.7
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5 min, 30% loss of activity at 53C
6.2
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5 min, 50% loss of activity at 53C
7
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5 min, 60% loss of activity at 53C
8
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5 min, 66% loss of activity at 53C
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
repeated freeze-thawing inactivates, sulfhydryl compounds do not stabilize
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D-xylulose, ATP, glycerol and EDTA stabilize
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EDTA and MgCl2 partially stabilize
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D233A
P09099
loss of activity
DELTAxyl3
Scheffersomyces stipitis FPL-YS30
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strain FPL-YS30, D-xylulokinase mutant with deletion of gene xyl3, proposal of an alternative pathway mediating xylose assimilation via arabinitol and ribulose-5-phosphate that bypasses the xylulokinase step
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additional information
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solubility of highly expressed mutant enzymes increased 8- to 77fold after introduction of GroEL-GroES into Escherichia coli
D6A
P09099
19fold decreased ATPase activity compared to the wild type enzyme
additional information
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Pichia stipitis xylulokinase gene (XYL3) is PCR-amplified from Pichia stipitis (strain UC7) genomic DNA, genes of interest are cloned downstream of a tac promoter and upstream of a transcription termination sequence into the medium copy vector pLOI3809, transforming plasmid into a DELTAxylB mutant Escherichia coli strain (PC07), determine whether an alternative xylulokinase (one not expected to act on xylitol) could functionally replace XylB in Escherichia coli, Xyl3 shares only 23% amino acid sequence identity with Escherichia coli XylB
additional information
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Escherichia coli xylulokinase gene xylB is PCR-amplified from Escherichia coli genomic DNA, genes of interest are cloned downstream of a tac promoter and upstream of a transcription termination sequence into the medium copy vector pLOI3809, transforming plasmid into a DELTAxylB mutant E. coli strain (PC07)
DELTAxyl3
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strain FPL-YS30, D-xylulokinase mutant with deletion of gene xyl3, proposal of an alternative pathway mediating xylose assimilation via arabinitol and ribulose-5-phosphate that bypasses the xylulokinase step
additional information
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enzyme disruption mutant, produces under aerobic as well as oxygen-limited conditions a negligible amount of ethanol and converts xylose mainly to xylitol. Xylose consumption increases 5fold under aerobic compared with oxygen-limited conditions, reaching a production of 26g/l of xylitonl