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Information on EC 2.7.1.134 - inositol-tetrakisphosphate 1-kinase and Organism(s) Homo sapiens and UniProt Accession Q13572

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EC Tree
IUBMB Comments
This enzyme also phosphorylates Ins(1,3,4)P3 on O-5 and O-6. The phosphotransfer from ATP to either inositol 1,3,4-trisphosphate or inositol 3,4,5,6-tetrakisphosphate appears to be freely reversible to the extent that the enzyme can act like an inositol polyphosphate phosphatase in the presence of ADP. It can also catalyse an isomerization between Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP. The enzymes from animals and plants also have the activity of EC 2.7.1.159, inositol-1,3,4-trisphosphate 5/6-kinase.
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Homo sapiens
UNIPROT: Q13572
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Synonyms
inositol 1,3,4-trisphosphate 5/6-kinase, osipk, itpk2, ositpk6, hvipk, inositol 1,3,4-trisphosphate 5/6 kinase, inositol tris/tetrakisphosphate kinase 1, inositol 1,4,5,6-tetrakisphosphate 3-kinase, inositol-tetrakisphosphate 1-kinase, inositol 1,3,4-trisphosphate 5/6-kinase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
inositol 1,3,4-trisphosphate 5/6 kinase
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1-myo-inositol-trisphosphate 6-kinase
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-
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1D-myo-inositol-tetrakisphosphate 1-kinase
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1D-myo-inositol-trisphosphate 5-kinase
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-
-
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ATP:1D-myo-inositol-1,3,4-trisphosphate 5-phosphoransferase
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-
-
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ATP:1D-myo-inositol-1,3,4-trisphosphate 6-phosphoransferase
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-
-
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inositol 1,4,5,6-tetrakisphosphate 3-kinase
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-
-
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inositol-trisphosphate 5-kinase
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-
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inositol-trisphosphate 6-kinase
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-
-
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ITPK1
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kinase, inositol 1,4,5,6-tetrakisphosphate 3- (phosphorylating)
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
ATP:1D-myo-inositol-3,4,5,6-tetrakisphosphate 1-phosphotransferase
This enzyme also phosphorylates Ins(1,3,4)P3 on O-5 and O-6. The phosphotransfer from ATP to either inositol 1,3,4-trisphosphate or inositol 3,4,5,6-tetrakisphosphate appears to be freely reversible to the extent that the enzyme can act like an inositol polyphosphate phosphatase in the presence of ADP. It can also catalyse an isomerization between Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP. The enzymes from animals and plants also have the activity of EC 2.7.1.159, inositol-1,3,4-trisphosphate 5/6-kinase.
CAS REGISTRY NUMBER
COMMENTARY hide
113356-25-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
ATP + myo-inositol-1,3,4,6-tetrakisphosphate
ADP + myo-inositol-1,3,4,5-tetrakisphosphate
show the reaction diagram
-
-
r
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,3,4,5-tetrakisphosphate
show the reaction diagram
ATP + myo-inositol-1,3,4-trisphosphate
ADP + myo-inositol-1,3,4,6-tetrakisphosphate
show the reaction diagram
enzyme has both inositol-1,3,4-trisphosphate 5-kinase and inositol-1,3,4-trisphosphate 6-kinase activity
5:1 ratio of inositol-tetrakis-1,3,4,6-phosphate and inositol-1,2,4,5-tetrakisphosphate
?
ATP + myo-inositol-3,4,6-trisphosphate
ADP + myo-inositol-1,3,4,6-tetrakisphosphate
show the reaction diagram
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
show the reaction diagram
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-
-
-
?
ATP + D-myo-inositol 1,3,4-triphosphate
?
show the reaction diagram
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-
-
-
?
additional information
?
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phosphate is transferred between inositol phosphates via ITPK1-bound nucleotide. Intersubstrate phosphate transfer explains how competing substrates are able to stimulate each others’ catalysis by ITPK1
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
phosphate is transferred between inositol phosphates via ITPK1-bound nucleotide. Intersubstrate phosphate transfer explains how competing substrates are able to stimulate each others’ catalysis by ITPK1
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
inositol-1,3,4-trisphosphate
activation of inositol-1,3,4,5,6-phosphate 1-phosphatase activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0003
myo-inositol-1,3,4-trisphosphate
pH 7.2, 37°C, recombinant enzyme
0.0001
myo-inositol-3,4,5,6-tetrakisphosphate
pH 7.2, 37°C, recombinant enzyme
0.0002
myo-inositol-3,4,6-trisphosphate
pH 7.2, 37°C, recombinant enzyme
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
deduced from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
3.6 kilobases transcript detected by Northern blot
Manually annotated by BRENDA team
3.6 kilobases transcript detected by Northern blot
Manually annotated by BRENDA team
3.6 kilobases transcript detected by Northern blot
Manually annotated by BRENDA team
3.6 kilobases transcript detected by Northern blot
Manually annotated by BRENDA team
3.6 kilobases transcript detected by Northern blot
Manually annotated by BRENDA team
3.6 kilobases transcript detected by Northern blot
Manually annotated by BRENDA team
3.6 kilobases transcript detected by Northern blot
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ITPK1_HUMAN
414
0
45621
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46000
x * 46000, deduced from nucleotide sequence
47000
x * 47000, deduced from nucleotide sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S356N
same catalytic activity as wild-type
H162D
-
loss-of-function for Ins(1,3,4)P3 in the H162D mutant is matched by a 200fold reduction in its Ins(1,3,4)P3 kinase activity. The H162D mutant dephosphorylats Ins(1,3,4,5,6)P5 almost twice as fast as the wild-type enzyme. The H162D mutant also displays very limited ability to transfer phosphate from Ins(1,3,4,5,6)P5 to Ins(1,3,4)P3 in the presence of physiological concentrations of nucleotide
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in colonic epithelial T84 cells
expression in Escherichia coli
expresssion in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wilson, M.P.; Majerus, P.W.
Isolation of inositol 1,3,4-trisphosphate 5/6-kinase, cDNA cloning, and expression of the recombinant enzyme
J. Biol. Chem.
271
11904-11910
1996
Bos taurus, Homo sapiens (Q13572), Homo sapiens
Manually annotated by BRENDA team
Yang, X.; Shears, B.S.
Multitasking in signal transduction by a promiscuous human ins(3,4,5,6)P4 1-kinase/ins(1,3,4)P3 5/6-kinase
Biochem. J.
351
551-555
2000
Homo sapiens (Q13572)
-
Manually annotated by BRENDA team
Ho, M.W.Y.; Yang, X.; Carew, M.A.; Zhang, T.; Hua, L.; Kwon, Y-U.; Chung, S-K.; Adelt, S.; Vogel, G.; Riley, a.M.; Potter, B.V.L.; Shears, S.B.
Regulation of ins(3,4,5,6)P4 signaling by a reversible kinase/phosphatase
Curr. Biol.
12
477-482
2002
Homo sapiens (Q13572)
Manually annotated by BRENDA team
Chamberlain, P.P.; Qian, X.; Stiles, A.R.; Cho, J.; Jones, D.H.; Lesley, S.A.; Grabau, E.A.; Shears, S.B.; Spraggon, G.
Integration of inositol phosphate signaling pathways via human ITPK1
J. Biol. Chem.
282
28117-28125
2007
Homo sapiens
Manually annotated by BRENDA team