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Information on EC 2.7.1.107 - diacylglycerol kinase (ATP) and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FFN7
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2.7.1.107 diacylglycerol kinase (ATP)IUBMB Comments
Involved in synthesis of membrane phospholipids and the neutral lipid triacylglycerol. Activity is stimulated by certain phospholipids [4,7]. In plants and animals the product 1,2-diacyl-sn-glycerol 3-phosphate is an important second messenger. cf. EC 2.7.1.174, diacylglycerol kinase (CTP).
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Word Map
- 2.7.1.107
-
phosphatidic
- phospholipase
- phospholipid
- pkc
- phosphatidylinositol
-
phorbol
- isozymes
- phosphoinositide
- phosphatidylcholine
- inositol
- ceramide
- lipase
-
arachidonic
- deoxyguanosine
- deoxycytidine
-
ef-hands
- phosphatidylserine
- 13-acetate
-
polyphosphoinositide
-
phosphohydrolase
-
prelabeled
- deoxynucleoside
- staurosporine
- sphingosine
- pip2
-
pleckstrin
- 4,5-bisphosphate
-
1,4,5-trisphosphate
- 4-phosphate
- deoxyribonucleoside
- analysis
- marcks
-
kinase-dead
- medicine
- phosphatidylethanol
-
transphosphatidylation
- dioctanoylglycerol
- hypospadias
- ptdoh
- 5-kinase
- agriculture
-
3hglycerol
- monoacylglycerols
-
anergy
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
dgk, diacylglycerol kinase, dag kinase, dg kinase, dgkzeta, dagk, dgkalpha, dgk-zeta, diglyceride kinase, dgkepsilon, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
DGK2
1,2-diacylglycerol kinase
-
-
-
-
adenosine 5'-triphosphate:1,2-diacylglycerol 3-phosphotransferase
-
-
-
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arachidonoyl-specific diacylglycerol kinase
-
-
-
-
ATP:diacylglycerol phosphotransferase
-
-
-
-
DAGK
-
-
-
-
DAGKalpha
-
-
-
-
DG kinase
-
-
-
-
DGK
DGK-alpha
-
-
-
-
DGK-theta
-
-
-
-
DGK4
DGKbeta
-
-
-
-
DGKdelta
-
-
-
-
DGKgamma
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-
-
-
DGKiota
-
-
-
-
DGKksi
-
-
-
-
diacylglycerol kinase
-
-
-
-
diacylglycerol kinase (ATP dependent)
-
-
-
-
diacylglycerol:ATP kinase
-
-
-
-
diglyceride kinase
-
-
-
-
kinase (phosphorylating), 1,2-diacylglycerol
-
-
-
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kinase, 1,2-diacylglycerol (phosphorylating)
-
-
-
-
sn-1,2-diacylglycerol kinase
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-
-
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DGK
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
ATP:1,2-diacyl-sn-glycerol 3-phosphotransferase
Involved in synthesis of membrane phospholipids and the neutral lipid triacylglycerol. Activity is stimulated by certain phospholipids [4,7]. In plants and animals the product 1,2-diacyl-sn-glycerol 3-phosphate is an important second messenger. cf. EC 2.7.1.174, diacylglycerol kinase (CTP).
CAS REGISTRY NUMBER
COMMENTARY
60382-71-0
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
i.e. phosphatidic acid
-
?
ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1-NBD-decanoyl-2-decanoyl-sn-glycerol
ADP + 1-NBD-decanoyl-2-decanoyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1-stearoyl-2-arachidonoyl-sn-glycerol
ADP + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1,2-dioleoyl-sn-glycerol
ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1-NBD-decanoyl-2-decanoyl-sn-glycerol
ADP + 1-NBD-decanoyl-2-decanoyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
-
?
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
i.e. phosphatidic acid
-
?
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
1,2-diacyl-sn-glycerol 3-phosphate is phosphatidic acid
-
ir
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
the enzyme binds and regulates signalling proteins which are activated by either diacylglycerol or phosphatidic acid
-
-
?
additional information
?
-
the isozyme DGK2 is involved in cold signal transduction
-
-
?
additional information
?
-
-
the isozyme DGK2 is involved in cold signal transduction
-
-
?
additional information
?
-
-
complex enzyme regulation, overview, the enzyme is involved in several processes such as cell growth, neuronal transmission, and cytoskeleton remodeling
-
-
?
additional information
?
-
isoform DGK4 has also guanylyl cyclase activity
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-
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1,2-diacyl-sn-glycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
-
-
?
ATP + 1,2-diacylglycerol
ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
the enzyme binds and regulates signalling proteins which are activated by either diacylglycerol or phosphatidic acid
-
-
?
additional information
?
-
the isozyme DGK2 is involved in cold signal transduction
-
-
?
additional information
?
-
-
the isozyme DGK2 is involved in cold signal transduction
-
-
?
additional information
?
-
-
complex enzyme regulation, overview, the enzyme is involved in several processes such as cell growth, neuronal transmission, and cytoskeleton remodeling
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
isozyme DGK2 activity is affected by salts and detergents, no inhibition by Ca2+
-
additional information
-
isozyme DGK2 activity is affected by salts and detergents, no inhibition by Ca2+
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
the isozyme DGK2 is induced by exposure to low temperatures, e.g. 4°C
-
additional information
-
the isozyme DGK2 is induced by exposure to low temperatures, e.g. 4°C
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
additional information
isozyme DGK2 expression in various tissues, expression pattern
additional information
-
isozyme DGK2 expression in various tissues, expression pattern
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
subcellular localization of isozymes, overview, the enzyme must undergo membrane translocation for access of diacylglycerols
-
-
fusion constructs of Green Fluorescent Protein to truncated forms of diacylglycerol kinase 1 and diacylglycerol kinase 2 missing the catalytic and accessory domains
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
isoform dgk2-deficient plants are gametophyte lethal, although parental single homozygous plants are viable. The dgk2/dgk4 double heterozygote shows defective pollen tube growth and seed development because of nonviable mutant gametes
physiological function
isoforms DGK2 is crucial for gametogenesis and biosynthesis of phosphatidylglycerol and phosphatidylinositol in the endoplasmic reticulum
malfunction
physiological function
malfunction
disruption of isoform DGK4 does not impair male gametophytic development but reduces in vivo performance and alters pollen tube Youngs modulus and adhesion properties
malfunction
isoform dgk4-deficient plants are gametophyte lethal, although parental single homozygous plants are viable. The dgk2/dgk4 double heterozygote shows defective pollen tube growth and seed development because of nonviable mutant gametes
physiological function
-
DGKs broadly regulate signaling events by virtue of their ability to provide 1,2-diacyl-sn-glycerol 3-phosphate (phosphatidic acid) for the synthesis of phosphatidylinositols
physiological function
-
in response to cold temperatures, there is a very fast accumulation of phosphatidic acid in Arabidopsis seedlings and leaf discs. Radiolabeling studies indicate a dominant role of diacylglycerol kinase under these conditions
physiological function
enzyme-deficient pollen tubes exhibit altered membrane recycling and phospholipid levels
physiological function
isoform DGK4 is crucial for gametogenesis and biosynthesis of phosphatidylglycerol and phosphatidylinositol in the endoplasmic reticulum
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). DGK2_ARATH
712
1
79405
Swiss-Prot
Secretory Pathway (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
140000
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE
79400
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 79400, about, sequence calculation, x * 140000, about, recombinant NusA/His6-tagged isozyme DGK2, SDS-PAGE
additional information
additional information
structural organization of isozymes containing a 1,2-diacylglycerol/phorbolester-binding domain 1, a zinc-finger dependent C1 domain, and a 1,2-diacylglycerol accessory domain, overview
additional information
-
structural organization of isozymes containing a 1,2-diacylglycerol/phorbolester-binding domain 1, a zinc-finger dependent C1 domain, and a 1,2-diacylglycerol accessory domain, overview
additional information
-
structure-function relationships of isozyme domain motifs, overview
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
expression of fusion constructs of Green Fluorescent Protein to truncated forms of diacylglycerol kinase 1 and diacylglycerol kinase 2 missing the catalytic and accessory domains. Fusion proteins are localized to the endoplasmic reticulum. Fusion constructs of N-terminal 50 amino acid residues of diacylglycerol kinase 1 and the 43 residues of diacylglycerol kinase 2 to the Yellow Fluorescent Protein alos localize to the endoplasmic reticulum
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CHAPS is the most effective detergent for isozyme DGK2 solubilization, NusA/His6-tagged fusion isozyme DGK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtDGK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as NusA/His6-tagged isozyme DGK2 in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Luo, B.; Regier, D.S.; Prescott, S.M.; Topham, M.K.
Diacylglycerol kinases
Cell. Signal.
16
983-989
2004
Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mammalia, no activity in Saccharomyces cerevisiae
Gomez-Merino, F.C.; Brearley, C.A.; Ornatowska, M.; Abdel-Haliem, M.E.; Zanor, M.I.; Mueller-Roeber, B.
AtDGK2, a novel diacylglycerol kinase from Arabidopsis thaliana, phosphorylates 1-stearoyl-2-arachidonoyl-sn-glycerol and 1,2-dioleoyl-sn-glycerol and exhibits cold-inducible gene expression
J. Biol. Chem.
279
8230-8241
2004
Arabidopsis thaliana (Q9FFN7), Arabidopsis thaliana
Vaultier, M.N.; Cantrel, C.; Guerbette, F.; Boutte, Y.; Vergnolle, C.; Cicek, D.; Bolte, S.; Zachowski, A.; Ruelland, E.
The hydrophobic segment of Arabidopsis thaliana cluster I diacylglycerol kinases is sufficient to target the proteins to cell membranes
FEBS Lett.
582
1743-1748
2008
Arabidopsis thaliana
Arisz, S.A.; Testerink, C.; Munnik, T.
Plant PA signaling via diacylglycerol kinase
Biochim. Biophys. Acta
1791
869-875
2009
Arabidopsis thaliana
Cai, J.; Abramovici, H.; Gee, S.H.; Topham, M.K.
Diacylglycerol kinases as sources of phosphatidic acid
Biochim. Biophys. Acta
1791
942-948
2009
Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Sus scrofa, Mus musculus (Q80UP3)
Arisz, S.A.; van Wijk, R.; Roels, W.; Zhu, J.K.; Haring, M.A.; Munnik, T.
Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase
Front. Plant Sci.
4
01
2013
Arabidopsis thaliana
Vaz Dias, F.; Serrazina, S.; Vitorino, M.; Marchese, D.; Heilmann, I.; Godinho, M.; Rodrigues, M.; Malho, R.
A role for diacylglycerol kinase 4 in signalling crosstalk during Arabidopsis pollen tube growth
New Phytol.
222
1434-1446
2019
Arabidopsis thaliana (Q1PDI2)
Angkawijaya, A.; Nguyen, V.; Gunawan, F.; Nakamura, Y.
A pair of Arabidopsis diacylglycerol kinases essential for gametogenesis and endoplasmic reticulum phospholipid metabolism in leaves and flowers
Plant Cell
32
2602-2620
2020
Arabidopsis thaliana (Q1PDI2), Arabidopsis thaliana (Q9FFN7)
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