Information on EC 2.6.1.9 - histidinol-phosphate transaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.6.1.9
-
RECOMMENDED NAME
GeneOntology No.
histidinol-phosphate transaminase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
show the reaction diagram
ping-pong bi bi mechanism
-
L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
show the reaction diagram
ping-pong bi bi mechanism
-
L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
show the reaction diagram
mechanism
-
L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
show the reaction diagram
mechanism via gem-diamino, aldimine, and ketimine reaction intermediates, active site structure analysis, overview
L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-histidine biosynthesis
-
-
histidine metabolism
-
-
Histidine metabolism
-
-
Tyrosine metabolism
-
-
Phenylalanine metabolism
-
-
Phenylalanine, tyrosine and tryptophan biosynthesis
-
-
Novobiocin biosynthesis
-
-
Tropane, piperidine and pyridine alkaloid biosynthesis
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
L-histidinol-phosphate:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminotransferase, histidinol phosphate
-
-
-
-
glutamic-imidazoleacetol phosphate transaminase
-
-
-
-
HisC
-
-
-
-
histidine:imidazoleacetol phosphate transaminase
-
-
-
-
histidinol phosphate aminotransferase
-
-
-
-
histidinol phosphate aminotransferase
-
-
histidinol-phosphate aminotransferase
-
-
-
-
histidinol-phosphate aminotransferase
-
IAP transaminase
-
-
-
-
imidazoleacetol phosphate transaminase
-
-
-
-
imidazolylacetolphosphate aminotransferase
-
-
-
-
imidazolylacetolphosphate transaminase
-
-
-
-
L-histidinol phosphate aminotransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9032-98-8
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
L-tyrosine + 2-oxoglutarate
show the reaction diagram
-
-
?
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-2-aminoadipate
L-histidinol phosphate + 2-oxoadipate
show the reaction diagram
Neurospora sp.
-
40% of the activity with L-glutamate
-
r
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-arginine
L-histidinol phosphate + 2-oxo-5-guanidinopentanoate
show the reaction diagram
Neurospora sp.
-
33% of the activity with L-glutamate
-
r
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
L-histidinol phosphate + 2-oxoglutarate
show the reaction diagram
histidine pathway
-
-
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
L-histidinol phosphate + 2-oxoglutarate
show the reaction diagram
-
eighth step in histidine biosynthesis
-
-
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamine
L-histidinol phosphate + 4-carbamoyl-2-oxobutanoate
show the reaction diagram
Neurospora sp.
-
4% of the activity with L-glutamate
-
r
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-histidine
L-histidinol phosphate + 3-(1H-imidazol-4-yl)-2-oxopropanoate
show the reaction diagram
Neurospora sp.
-
30% of the activity with L-glutamate
-
r
3-indole-2-oxopropanoate + L-glutamate
L-tryptophan + 2-oxoglutarate
show the reaction diagram
-
-
?
4-methyl-2-oxopentanoate + L-alanine
L-leucine + pyruvate
show the reaction diagram
-
-
?
4-methyl-2-oxopentanoate + L-asparagine
L-leucine + 2-oxosuccinamic acid
show the reaction diagram
-
-
?
4-methyl-2-oxopentanoate + L-glutamate
L-leucine + 2-oxoglutarate
show the reaction diagram
-
-
?
beta-chloro-L-alanine
pyruvate + NH3 + Cl-
show the reaction diagram
-
-
-
?
L-glutarate + 2-oxobutanoate
2-oxoglutarate + 2-aminobutanoate
show the reaction diagram
-
-
?
L-histidine + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
?
L-histidinol phosphate + 2-oxo-5-guanidinopentanoate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-arginine
show the reaction diagram
Neurospora sp.
-
-
-
r
L-histidinol phosphate + 2-oxoadipate
3-(imidazol-4-yl)-2-oxopropyl phosphate + 2-aminoadipate
show the reaction diagram
Neurospora sp.
-
70% of the activity with 2-oxoglutarate
-
r
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
show the reaction diagram
-
-
-
?
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
show the reaction diagram
-
-
-
?
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
show the reaction diagram
-
-
-
?
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
show the reaction diagram
-
-
-
?
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
show the reaction diagram
-
-
-
?
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
show the reaction diagram
-
-
-
?
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
show the reaction diagram
-
i.e. imidazoleacetol phosphate + L-Glu
?
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Glu
show the reaction diagram
Neurospora sp.
-
-
i.e. imidazoleacetol phosphate + L-Glu
r
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
show the reaction diagram
-
-
r
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
show the reaction diagram
-
-
?
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
show the reaction diagram
catalytic reaction pathway, histidine biosynthesis
-
r
L-histidinol phosphate + 4-hydroxyphenylpyruvate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-tyrosine
show the reaction diagram
-
-
-
?
L-histidinol phosphate + oxaloacetate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-aspartate
show the reaction diagram
-
-
-
?
L-histidinol phosphate + phenylpyruvate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-phenylalanine
show the reaction diagram
-
-
-
?
L-histidinol phosphate + pyruvate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-Ala
show the reaction diagram
-
-
-
?
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
-
-
?
L-phenylalanine + 2-oxoglutarate
phenylpyruvate + L-glutamate
show the reaction diagram
-
-
-
?
L-phenylalanine + 2-oxoglutarate
phenylpyruvate + L-glutamate
show the reaction diagram
-
-
-
?
L-phenylalanine + 2-oxoglutarate
phenylpyruvate + L-glutamate
show the reaction diagram
low activity with
-
r
L-tryptophan + 2-oxoglutarate
3-indole-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
-
?
L-tryptophan + 2-oxoglutarate
3-indole-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
r
L-tyrosine + 2-oxoglutarate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamate
show the reaction diagram
-
-
r
phenylpyruvate + L-glutamate
L-phenylalanine + 2-oxoglutarate
show the reaction diagram
-
-
?
L-tyrosine + 2-oxoglutarate
3-(4-hydroxyphenyl)-2-oxopropanoate + glutamate
show the reaction diagram
-
-
-
?
additional information
?
-
Neurospora sp.
-
no substrate: oxaloacetate, pyruvate
-
-
-
additional information
?
-
enzyme might also catalyze the transamination reaction with other substrates generating aromatic amino acids in vivo, no activity with L-histidine
-
-
-
additional information
?
-
-
no activity with L-histidine
-
-
-
additional information
?
-
-
complete knockout of AtHPA1 is lethal for embryo
-
-
-
additional information
?
-
no substrate: 3-methyl-2-oxopentanoate, 3-methyl-2-oxobutanoate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
L-histidinol phosphate + 2-oxoglutarate
show the reaction diagram
P06986
histidine pathway
-
-
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
L-histidinol phosphate + 2-oxoglutarate
show the reaction diagram
-
eighth step in histidine biosynthesis
-
-
L-histidinol phosphate + 2-oxoglutarate
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate
show the reaction diagram
Q9X0D0
catalytic reaction pathway, histidine biosynthesis
-
r
additional information
?
-
Q9X0D0
enzyme might also catalyze the transamination reaction with other substrates generating aromatic amino acids in vivo
-
-
-
additional information
?
-
-
complete knockout of AtHPA1 is lethal for embryo
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
0.0022 mM; a pyridoxal phosphate protein
pyridoxal 5'-phosphate
Neurospora sp.
-
a pyridoxal phosphate protein; Km: 0.0012 mM
pyridoxal 5'-phosphate
-
1 pyridoxal phosphate group associated with each molecule of enzyme; a pyridoxal phosphate protein
pyridoxal 5'-phosphate
-
a pyridoxal phosphate protein; sequence of pyridoxal phosphate binding site
pyridoxal 5'-phosphate
-
2 mol of pyridoxal phosphate per mol of enzyme; a pyridoxal phosphate protein
pyridoxal 5'-phosphate
-
removal of cofactor triggers irreversible denaturation
pyridoxal 5'-phosphate
covalently bound
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Co2+
Neurospora sp.
-
0.004 M, 80% inhibition
Cu2+
Neurospora sp.
-
0.004 M, 80% inhibition
hydroxylamine
Neurospora sp.
-
0.004 M, 80% inhibition
iodoacetate
Neurospora sp.
-
0.004 M, 95% inhibition
L-glutamate
-
competitive to tyrosine
Semicarbazide
Neurospora sp.
-
0.004 M, 80% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
omega-Methylpyridoxal phosphate
Neurospora sp.
-
can replace pyridoxal phosphate, Km: 0.004 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.2
2-oxoglutarate
Neurospora sp.
-
pH 8.1, 37C
7.1
2-oxoglutarate
-
-
0.15
L-Histidinol phosphate
-
-
0.17
L-Histidinol phosphate
-
pH 7, 37C
0.8
L-Histidinol phosphate
pH 8.0, 20C
0.89
L-Histidinol phosphate
wild-type
1.1
L-Histidinol phosphate
Neurospora sp.
-
pH 8.1, 37C
1.3
L-Histidinol phosphate
mutant Y123F
2.2
L-Histidinol phosphate
mutant Y257F
6.7
L-Histidinol phosphate
mutant Y21F
9.1
L-Histidinol phosphate
mutant N99D
32.8
L-leucine
mutant Y257F
53.4
L-leucine
mutant Y123F
55.8
L-leucine
mutant N99D
80.8
L-leucine
mutant Y21F
94.9
L-leucine
wild-type
2.5
L-phenylalanine
-
-
22.2
L-phenylalanine
mutant Y257F
25.5
L-phenylalanine
mutant Y21F
27.5
L-phenylalanine
mutant Y123F
38
L-phenylalanine
pH 8.0, 20C
71.4
L-phenylalanine
mutant N99D
106
L-phenylalanine
wild-type
3.4
L-tryptophan
pH 8.0, 20C
0.71
L-tyrosine
-
-
2.3
L-tyrosine
pH 8.0, 20C
43.48
phenylalanine
-
pH 7, 37C
3.39
tyrosine
-
pH 7, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.046
L-Histidinol phosphate
Thermotoga maritima
Q9X0D0
pH 8.0, 25C
0.39
L-Histidinol phosphate
Corynebacterium glutamicum
Q9KJU4
mutant Y123F
0.51
L-Histidinol phosphate
Corynebacterium glutamicum
Q9KJU4
mutant Y257F
1.18
L-Histidinol phosphate
Corynebacterium glutamicum
Q9KJU4
wild-type
1.86
L-Histidinol phosphate
Corynebacterium glutamicum
Q9KJU4
mutant N99D
1.93
L-Histidinol phosphate
Corynebacterium glutamicum
Q9KJU4
mutant Y21F
0.66
L-leucine
Corynebacterium glutamicum
Q9KJU4
mutant Y123F
0.69
L-leucine
Corynebacterium glutamicum
Q9KJU4
mutant Y257F
1.6
L-leucine
Corynebacterium glutamicum
Q9KJU4
mutant N99D
1.72
L-leucine
Corynebacterium glutamicum
Q9KJU4
mutant Y21F
3.27
L-leucine
Corynebacterium glutamicum
Q9KJU4
wild-type
0.009
L-phenylalanine
Thermotoga maritima
Q9X0D0
pH 8.0, 25C
0.59
L-phenylalanine
Corynebacterium glutamicum
Q9KJU4
mutant Y123F
0.68
L-phenylalanine
Corynebacterium glutamicum
Q9KJU4
mutant Y257F
0.87
L-phenylalanine
Corynebacterium glutamicum
Q9KJU4
mutant Y21F
2.22
L-phenylalanine
Corynebacterium glutamicum
Q9KJU4
mutant N99D
4.1
L-phenylalanine
Corynebacterium glutamicum
Q9KJU4
wild-type
0.014
L-tryptophan
Thermotoga maritima
Q9X0D0
pH 8.0, 25C
0.043
L-tyrosine
Thermotoga maritima
Q9X0D0
pH 8.0, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.01266
protein extract after expression in wild-type Escherichia coli DH5alpha
0.1
substrates 4-methyl-2-oxopentanoate, L-alanine
0.2
substrates L-glutarate, 2-oxobutanoate
0.3
substrates 3-indole-2-oxopropanoate, L-glutamate
0.4
substrates 4-methyl-2-oxopentanoate, L-asparagine
0.5
substrates 3-(4-hydroxyphenyl)-2-oxopropanoate, L-glutamate
1.4
substrates 4-methyl-2-oxopentanoate, L-glutamate
1.5
substrates phenylpyruvate, L-glutamate
2.8
substrates L-histidinol phosphate, 2-oxoglutarate
7
-
2-oxoglutarate + histidinol-phosphate
7.11
-
4-hydroxyphenylpyruvate + histidinol-phosphate
11.6
-
2-oxoglutarate + phenylalanine; 2-oxoglutarate + tyrosine
60.3
Neurospora sp.
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.1
Neurospora sp.
-
diphosphate buffer
8.4
Neurospora sp.
-
diethanolamine buffer
8.5
Neurospora sp.
-
triethanolamine buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5 - 9
Neurospora sp.
-
pH 7.5: about 85% of activity maximum, pH 9.0: about 50% of activity maximum, diphosphate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
Neurospora sp.
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Geobacter metallireducens (strain GS-15 / ATCC 53774 / DSM 7210)
Listeria innocua serovar 6a (strain CLIP 11262)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Rhizobium meliloti (strain 1021)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
33000
-
gel filtration
636659
37000
-
sedimentation equilibrium
637149
45400
calculated from nucleotide sequence
637150
53000
SDS-PAGE
637150
59000
-
equilibrium centrifugation, pyridoxal phosphate analysis, spectrophotometric assay, phosphate analysis, viscometry
637143
60000
-
dynamic light scattering experiments
637154
74000
-
sedimentation equilibrium measurement
637146
85000
-
gel filtration
637151
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
-
2 * 25300-37000, nonidentical, equilibrium centrifugation in guanidine-HCl, sedimentation velocity in guanidine-HCl, viscosity analysis in guanidine-HCl
dimer
-
2 * 40000, homodimer, SDS-PAGE
dimer
-
homodimer, crystallization experiments
dimer
-
homodimer, dynamic light scattering experiments
additional information
-
marked tendency to polymerize upon ageing
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
apo histidinol-phosphate aminotransferase the internal PLP aldimine adduct and a pyridoxamine 5'-phosphate enzyme complex at resolutions of 2.2 A, 2.1 A and 1.8 A, respectively. The hydrogen bond between the side chain of residue Y21 and the phosphate group of histidinol phosphate is important for recognition of the natural substrate and discrimination against other potential amino donors. Residue N99 does not contribute to the specific recognition of the amino-acid donor. Residues Y123 and Y257 interact with the substrate through van der Waals-interactions
hanging drop vapor diffusion method
-
native and complexed with L-histidinol phosphate or N-(5'-phosphopyridoxyl)-L-glutamate, hanging drop vapor diffusion method
-
diffraction-quality crystals are grown using the hanging-drop vapour-diffusion technique. X-ray diffraction data are recorded to 2.45 A resolution
-
wild-type and selenomethionine-labeled enzyme, with or without bound pyridoxal 5'-phosphate or pyridoxamine 5'-phosphate, sitting drop vapour diffusion method, 0.001 ml equal volumes of protein and reservoir solutions, 20C, 50% v/v ethylene glycol, 5% w/v PEG 1000, sodium acetate, pH 5.1, 2-3 weeks, X-ray diffraction structure determination and analysis at 3.5 A resolution
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 8.5
-
stable overnight
636659
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
-
holoenzyme and apoenzyme are stable for 15 min
637149
45
-
holoenzyme loses 15% activity after 5 min, apoenzyme loses 70% activity after 5 min
637149
50
-
no loss of activity for 2 min
637151
55
-
4 min, more than 90% loss of activity, 10 min, in presence of pyridoxal 5'-phosphate, 75% loss of activity
636659
55
-
enzyme loses activity when incubated above 55C for 2 min
637151
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, 1 month, 30% loss of activity
-
-20C, stable for several months
Neurospora sp.
-
-20C over night and thawing on ice: 98% loss of activity
-
-80C no loss of activity after several months when stored in 10% glycerol
-
-80C over night and thawing on ice: 58% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
wild-type and mutant enzymes
-
purified to homogeneity using nickel-nitrilotriacetic acid metal-affinity and gel filtration chromatography
-
C141-T1710, C141
Neurospora sp.
-
from derepressed histidine auxotrophs, 95% pure
-
use of omega-aminoalkylagaroses, 15fold purification in one step
-
recombinant wild-type and selenomethionine-labeled enzyme from Escherichia coli strain BL21(DE3) and B834(DE3), respectively
recombinant protein
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
HisC2 is overexpressed in Mycobacterium smegmatis
-
expression of wild-type and selenomethionine-labeled enzyme in Escherichia coli strain BL21(DE3) and B834(DE3), respectively
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
N99D
6fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate
Y123F
4fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate
Y21F
10fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate
Y257F
5fold reduction in ratio kcat to Km for substrates L-histidinol phosphate, 2-oxoglutarate
K214A
-
increased pKa
N157A
-
increased pKa
N157A/R335L
-
increased pKa
R335L
-
increased pKa
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
holoenzyme and apoenzyme can be constituted in vitro with pyridoxal 5'-phosphate
-