Information on EC 2.6.1.86 - 2-amino-4-deoxychorismate synthase

New: Word Map on EC 2.6.1.86
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
2.6.1.86
-
RECOMMENDED NAME
GeneOntology No.
2-amino-4-deoxychorismate synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2S)-2-amino-4-deoxychorismate + L-glutamate = chorismate + L-glutamine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleophilic substitution
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
phenazine-1-carboxylate biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(2S)-2-amino-4-deoxychorismate:2-oxoglutarate aminotransferase
Requires Mg2+. The reaction occurs in the reverse direction to that shown above. In contrast to most anthranilate-synthase I (ASI) homologues, this enzyme is not inhibited by tryptophan. In Streptomyces globisporus, the sequential action of this enzyme and EC 1.3.99.24, 2-amino-4-deoxychorismate dehydrogenase, leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 [1,2]. In certain Pseudomonads the enzyme participates in the biosynthesis of phenazine, a precursor for several compounds with antibiotic activity [3,4].
CAS REGISTRY NUMBER
COMMENTARY hide
152060-54-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chorismate + L-glutamine
(2S)-2-amino-4-deoxychorismate + L-glutamate
show the reaction diagram
L-glutamine + chorismate
(S)-2-amino-2-deoxyisochorismate + glutamate
show the reaction diagram
-
-
-
?
L-glutamine + chorismate
(S)-2-amino-2-deoxyisochorismate + L-glutamate
show the reaction diagram
additional information
?
-
-
the enzyme participates in the biosynthesis of phenazine, a precursor for several compounds with antibiotic activity
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
chorismate + L-glutamine
(2S)-2-amino-4-deoxychorismate + L-glutamate
show the reaction diagram
-
the net conversion of chorismate to 3-enolpyruvoylanthranilate by the tandem action of SgcD and SgcG establishes a new branching point in chorismate metabolism leading to synthesis of C-1027, an enediyne antitumor antibiotic
-
-
?
additional information
?
-
-
the enzyme participates in the biosynthesis of phenazine, a precursor for several compounds with antibiotic activity
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mn2+
-
reduced activity
Ni2+
-
reduced activity
Zn2+
-
reduced activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0024 - 0.02
chorismate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 2.2
chorismate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
optimal activity at low pH values between pH 6.5 and pH 8.5
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor diffusion method, PEG 3350
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography (Ni2+), cleavage of the tag, gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged protein expressed in Escherichia coli BL21(DE3)
-
the gene for SgcD is cloned into pET-30 Xa/LIC and expressed in Escherichia coli
-