Information on EC 2.6.1.6 - leucine transaminase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
2.6.1.6
-
RECOMMENDED NAME
GeneOntology No.
leucine transaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
leucine biosynthesis
-
leucine degradation I
-
leucine degradation III
-
Metabolic pathways
-
Pantothenate and CoA biosynthesis
-
Valine, leucine and isoleucine biosynthesis
-
Valine, leucine and isoleucine degradation
-
SYSTEMATIC NAME
IUBMB Comments
L-leucine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. This enzyme differs from EC 2.6.1.42, branched-chain-amino-acid transaminase, in that it does not act on L-valine or L-isoleucine, although it does act on L-methionine. The mitochondrial form from rat liver differs in physical characteristics from the cytoplasmic form.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
L-leucine aminotransferase
-
-
-
-
leucine 2-oxoglutarate transaminase
-
-
-
-
leucine aminotransferase
-
-
-
-
leucine-alpha-ketoglutarate transaminase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-37-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Amaranthus dubius
-
-
-
Manually annotated by BRENDA team
soybean, var. Kali tur
-
-
Manually annotated by BRENDA team
rat, strain Wistar
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
-
-
?
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
-
-
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
-
-
-
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
-
-
r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
-
-
?, r
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly
-
?
L-leucine + 4-methyl-2-oxopentanoate
4-methyl-2-oxopentanoate + L-leucine
show the reaction diagram
-
-
-
-
?
L-leucine + oxaloacetate
4-methyl-2-oxopentanoate + L-aspartate
show the reaction diagram
-
-
-
-
?
L-methionine + 2-oxoglutarate
4-methylsulfanyl-2-oxobutanoate + L-glutamate
show the reaction diagram
-
-
-
-
?, r
L-methionine + 2-oxoglutarate
4-methylsulfanyl-2-oxobutanoate + L-glutamate
show the reaction diagram
-
mitochondrial isoenzyme
-
-
r
additional information
?
-
-
specific for leucine, isoleucine or valine, no other amino acid would serve as an amino donor, pyruvate is no amino acid acceptor
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
-
-
?
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
-
-
?
L-leucine + 2-oxoglutarate
4-methyl-2-oxopentanoate + L-glutamate
show the reaction diagram
-
leucine metabolism and hence of cholesterogenesis and ketogenesis, not present in fetal rat liver, but after birth activity appears and increases rapidly
-
?
L-methionine + 2-oxoglutarate
4-methylsulfanyl-2-oxobutanoate + L-glutamate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
Km 0.004 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
slight inhibition
Adipic acid
-
-
Fumaric acid
-
-
Glutaric acid
-
-
hydroxylamine
-
inhibits the aldehyde form of the enzyme while the amino form is found to be inert
Maleate
-
-
-
Maleic acid
-
-
p-chloromercuribenzoate
-
1.0 mM, complete inhibition
phenylhydrazine
-
-
potassium cyanide
-
-
Sodium bisulfite
-
-
succinic acid
-
-
Thiosemicarbazide
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.065
-
2-oxoglutarate
-
pH 8.2, 37C
2.3
-
2-oxoglutarate
-
isozyme LAT II, pH 9, 37C
3.3
-
2-oxoglutarate
-
isozyme LAT I, pH 9, 37C
1.67
-
L-leucine
-
isozyme LAT II, pH 9, 37C
2.5
-
L-leucine
-
isozyme LAT I, pH 9, 37C
25
-
leucine
-
pH 8.2, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.4
-
-
isoenzyme LAT I
8.7
-
-
isoenzyme LAT II
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.7
-
Amaranthus dubius
-
-
8.9
-
-
-
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Amaranthus dubius
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
predominantly found in mitochondrion
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
65000
-
-
gel filtration
68000
-
-
gel filtration, isoenzyme LAT I
93300
-
-
gel filtration, isoenzyme LAT II
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
45
-
-
quite stable on heating for 1 h
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
guanidine hydrochloride
Amaranthus dubius
-
shows sensitivity towards
urea
Amaranthus dubius
-
shows sensitivity towards
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, concentrated enzyme, activity is stable for 2 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partially, 2 isoenzymes, LAT I and LAT II
Amaranthus dubius
-
partially, 2 isoenzymes, LAT I and LAT II
-