Information on EC 2.6.1.51 - serine-pyruvate transaminase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY
2.6.1.51
-
RECOMMENDED NAME
GeneOntology No.
serine-pyruvate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-serine + pyruvate = 3-hydroxypyruvate + L-alanine
show the reaction diagram
A pyridoxal-phosphate protein; A pyridoxal-phosphate protein.; a pyridoxal-phosphate protein. The liver enzyme may be identical with EC 2.6.1.44 alanine-glyoxylate transaminase
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Glycine, serine and threonine metabolism
-
Metabolic pathways
-
SYSTEMATIC NAME
IUBMB Comments
L-serine:pyruvate aminotransferase
A pyridoxal-phosphate protein. The liver enzyme may be identical with EC 2.6.1.44 alanine-glyoxylate transaminase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
alanine:glyoxylate aminotransferase-1
-
-
Dm-Spat
Q9W3Z3
-
hydroxypyruvate:L-alanine transaminase
-
-
-
-
isoenzyme 1 of histidine-pyruvate aminotransferase
-
-
isoenzyme 1 of histidine-pyruvate aminotransferase
Rattus norvegicus Donryu
-
-
-
serine:pyruvate aminotransferase
Q97VM5
-
serine:pyruvate aminotransferase
Q97VM5
-
-
serine:pyruvate/alanine:glyoxylate aminotransferase
-
-
serine:pyruvate/alanine:glyoxylate aminotransferase
-
-
serine:pyruvate/alanine:glyoxylate aminotransferase
-
-
serine:pyruvate/alanine:glyoxylate aminotransferase
-
-
serine:pyruvate/alanine:glyoxylate aminotransferase
Rattus norvegicus Wistar
-
;
-
SPT
-
-
-
-
SPT/AGT
Rattus norvegicus Wistar
-
-
-
SPT10
-
recombinant enzyme
CAS REGISTRY NUMBER
COMMENTARY
9030-88-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
age 3 and 30 months, no change in enzyme activtiy with age or diet
-
-
Manually annotated by BRENDA team
mouse
-
-
Manually annotated by BRENDA team
rainbow trout
-
-
Manually annotated by BRENDA team
rabbit, Japanese white
-
-
Manually annotated by BRENDA team
kidney bean, L. cv. Red Kidney
-
-
Manually annotated by BRENDA team
var. Alaska
-
-
Manually annotated by BRENDA team
bifunctional serine pyruvate/alanine:glyoxylate aminotransferase
-
-
Manually annotated by BRENDA team
Donryu strain
-
-
Manually annotated by BRENDA team
strain Wistar
-
-
Manually annotated by BRENDA team
Rattus norvegicus Donryu
Donryu strain
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
strain Wistar
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobutyrate + glyoxylate
2-oxobutanoate + glycine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
5-hydroxytryptophan + pyruvate
3-(5-hydroxyindole)-2-oxopropanoate + alanine
show the reaction diagram
-
-
-
-
?
5-hydroxytryptophan + pyruvate
3-(5-hydroxyindole)-2-oxopropanoate + alanine
show the reaction diagram
-
-
-
-
?
arginine + pyruvate
5-guanidino-2-oxopentanoate + L-alanine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
asparagine + pyruvate
2-oxosuccinamate + L-alanine
show the reaction diagram
-
-
-
-
asparagine + pyruvate
2-oxosuccinamate + L-alanine
show the reaction diagram
-
-
-
-
asparagine + pyruvate
2-oxosuccinamate + L-alanine
show the reaction diagram
-
-
-
-
cysteine + pyruvate
3-mercapto-2-oxo-propanoate + L-alanine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
glutamic acid + 3-hydroxypyruvate
2-oxo-1,5-pentandioate + serine
show the reaction diagram
-
-
-
-
r
glutamine + phenylpyruvate
4-carbamoyl-2-oxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
?
glutamine + pyruvate
4-carbamoyl-2-oxobutanoate + L-alanine
show the reaction diagram
-
-
-
?
glutamine + pyruvate
4-carbamoyl-2-oxobutanoate + L-alanine
show the reaction diagram
-
-
-
?
glutamine + pyruvate
4-carbamoyl-2-oxobutanoate + L-alanine
show the reaction diagram
-
-
-
?
glycine + 3-hydroxypyruvate
glyoxylate + L-serine
show the reaction diagram
-
-
-
-
r
glycine + 3-hydroxypyruvate
glyoxylate + L-serine
show the reaction diagram
-
-
-
-
r
glycine + 3-hydroxypyruvate
L-serine + glyoxylate
show the reaction diagram
-
-
-
r
glycine + 3-hydroxypyruvate
L-serine + glyoxylate
show the reaction diagram
-
-
-
r
glycine + pyruvate
glyoxylate + L-alanine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
histidine + glyoxylate
3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
histidine + pyruvate
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
show the reaction diagram
-
-
-
-
?
histidine + pyruvate
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
show the reaction diagram
-
-
-
?
histidine + pyruvate
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
show the reaction diagram
-
-
-
-
?
histidine + pyruvate
3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
isoleucine + pyruvate
3-methyl-2-oxopentanoate + alanine
show the reaction diagram
-
-
-
?
L-alanine + 3-hydroxypyruvate
pyruvate + L-serine
show the reaction diagram
-
-
-
-
r
L-alanine + 3-hydroxypyruvate
pyruvate + L-serine
show the reaction diagram
-
-
-
-
r
L-alanine + 3-hydroxypyruvate
pyruvate + L-serine
show the reaction diagram
-
-
-
-
r
L-alanine + 3-hydroxypyruvate
pyruvate + L-serine
show the reaction diagram
-
-
-
-
r
L-alanine + glyoxylate
pyruvate + glycine
show the reaction diagram
-
-
-
?
L-alanine + glyoxylate
pyruvate + glycine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
L-alanine + phenylpyruvate
pyruvate + L-phenylalanine
show the reaction diagram
-
-
-
?
L-asparagine + phenylpyruvate
2-oxosuccinamate + L-phenylalanine
show the reaction diagram
-
-
-
-
L-leucine + phenylpyruvate
4-methyl-2-oxopentanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
L-leucine + phenylpyruvate
4-methyl-2-oxopentanoate + L-phenylalanine
show the reaction diagram
-
-
-
?
L-leucine + phenylpyruvate
4-methyl-2-oxopentanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
L-leucine + pyruvate
4-methyl-2-oxopentanoate + L-alanine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
L-methionine + glyoxylate
4-methylsulfanyl-2-oxobutanoate + glycine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
L-methionine + phenylpyruvate
4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
show the reaction diagram
Rattus norvegicus, Rattus norvegicus Donryu
-
-
-
?
L-methionine + pyruvate
4-methylsulfanyl-2-oxobutanoate + L-alanine
show the reaction diagram
-
-
-
?
L-methionine + pyruvate
4-methylsulfanyl-2-oxobutanoate + L-alanine
show the reaction diagram
-
-
-
?
L-methionine + pyruvate
4-methylsulfanyl-2-oxobutanoate + L-alanine
show the reaction diagram
-
-
-
?
L-methionine + pyruvate
4-methylsulfanyl-2-oxobutanoate + L-alanine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
L-phenylalanine + glyoxylate
phenylpyruvate + glycine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
L-phenylalanine + pyruvate
phenylpyruvate + L-alanine
show the reaction diagram
-
-
-
-
?
L-phenylalanine + pyruvate
phenylpyruvate + L-alanine
show the reaction diagram
-
-
-
?
L-phenylalanine + pyruvate
phenylpyruvate + L-alanine
show the reaction diagram
-
-
-
-
?
L-phenylalanine + pyruvate
phenylpyruvate + L-alanine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
L-phenylalanine + pyruvate
phenylpyruvate + L-alanine
show the reaction diagram
Rattus norvegicus Donryu
-
-
-
?
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
-
-
-
-
r
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
-
-
-
-
r
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
-
-
-
r
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
-
-
-
-
r
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
-
-
-
-
r
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
-
-
-
r
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
-
-
-
-
r
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
-
-
-
-
r
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
-, Q9W3Z3
-
-
-
r
L-serine + glyoxylate
glycine + 3-hydroxypyruvate
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
r
L-serine + oxaloacetate
3-hydroxypyruvate + L-aspartate
show the reaction diagram
-
-
-
?
L-serine + phenylpyruvate
3-hydroxypyruvate + L-phenylalanine
show the reaction diagram
-
-
-
r
L-serine + phenylpyruvate
3-hydroxypyruvate + L-phenylalanine
show the reaction diagram
-
little activity detected
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-, Q9W3Z3
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
Q97VM5, -
-
-
-
?
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-, Q9W3Z3
glyoxylate detoxification, physiological role in tryptophan catabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
L-serine metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
L-serine metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
L-serine metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
L-serine metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
gluconeogenesis from serine
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
gluconeogenesis from serine
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
gluconeogenesis from serine
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
gluconeogenesis from serine
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
glyoxylate metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
glyoxylate metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
Rattus norvegicus Donryu
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
Rattus norvegicus Wistar
-
gluconeogenesis from serine, glyoxylate metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
Q97VM5
-
-
-
?
L-tryptophan + glyoxylate
3-indole-2-oxopropanoate + glycine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
L-tryptophan + pyruvate
3-indole-2-oxopropanoate + L-alanine
show the reaction diagram
-
-
-
-
?
L-tyrosine + glyoxylate
3-(4-hydroxyphenyl)-2-oxopropanoate + glycine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
L-tyrosine + pyruvate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine
show the reaction diagram
-
-
-
-
?
L-tyrosine + pyruvate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine
show the reaction diagram
-
-
-
-
?
L-tyrosine + pyruvate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine
show the reaction diagram
-, Q9W3Z3
-
-
-
?
L-tyrosine + pyruvate
3-(4-hydroxyphenyl)-2-oxopropanoate + L-alanine
show the reaction diagram
Rattus norvegicus Donryu
-
-
-
-
?
ornithine + phenylpyruvate
5-amino-2-oxopentanoate + L-phenylalanine
show the reaction diagram
-
-
-
?
ornithine + pyruvate
5-amino-2-oxopentanoate + L-alanine
show the reaction diagram
-
-
-
?
threonine + phenylpyruvate
3-hydroxy-2-oxobutanoate + L-phenylalanine
show the reaction diagram
-
-
-
-
?
valine + pyruvate
3-methyl-2-oxobutanoate + alanine
show the reaction diagram
-
-
-
-
?
lysine + pyruvate
6-amino-2-oxohexanoate + alanine
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
D-alanine is no substrate
-
-
-
additional information
?
-
-
identical with histidine-pyruvate aminotransferase isoenzyme 1, isoleucine and oxoglutarate are no substrates
-
-
-
additional information
?
-
-
highly specific for serine as the amino donor
-
-
-
additional information
?
-
-
highly specific for serine as the amino donor
-
-
-
additional information
?
-
-
class IV aminotransferase, identical with EC 2.6.1.44
-
-
-
additional information
?
-
-
class IV aminotransferase, identical with EC 2.6.1.44
-
-
-
additional information
?
-
-, Q9W3Z3
amino-adipate, asparagine, aspartate, glutamate, isoleucine, lysine, threonine and valine are no substrates
-
-
-
additional information
?
-
-
2-oxoglutarate, indol-3-ylpyruvate and p-hydroxyphenylpyruvate are no substrates
-
-
-
additional information
?
-
-
enzyme converts L-hydroxyproline-derived glyoxylate into glycine in situ, preventing undesirable overflow into the production of oxalate
-
-
-
additional information
?
-
Rattus norvegicus Donryu
-
identical with histidine-pyruvate aminotransferase isoenzyme 1, isoleucine and oxoglutarate are no substrates
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-alanine + 3-hydroxypyruvate
pyruvate + L-serine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-, Q9W3Z3
glyoxylate detoxification, physiological role in tryptophan catabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
L-serine metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
L-serine metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
L-serine metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
L-serine metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
gluconeogenesis from serine
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
gluconeogenesis from serine
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
gluconeogenesis from serine
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
gluconeogenesis from serine
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
glyoxylate metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
-
glyoxylate metabolism
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
Rattus norvegicus Donryu
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
r
L-serine + pyruvate
3-hydroxypyruvate + L-alanine
show the reaction diagram
Rattus norvegicus Wistar
-
gluconeogenesis from serine, glyoxylate metabolism
-
-
r
additional information
?
-
-
enzyme converts L-hydroxyproline-derived glyoxylate into glycine in situ, preventing undesirable overflow into the production of oxalate
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
Q97VM5, -
the structure of the protein is a homodimer that adopts the type I fold of pyridoxal 5'-phosphate-dependent aminotransferases. The structure reveals the PLP cofactor covalently bound in the active site to the active-site lysine in the internal aldimine form
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
histidine
-
competitive inhibitor of serine transamination
hydroxylamine
-
-
Isonicotinic acid hydrazide
-
-
Isonicotinic acid hydrazide
-
-
N-ethylmaleimide
-
-
serine
-
competitive inhibitor of histidine transamination
additional information
-
less sensitive to N-ethylmaleimide and p-chloromercuribenzoate, not inhibited by NH4+
-
additional information
-
not inhibited by isonicotinic acid hydrazide
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Glucagon
-
mitochondrial enzyme is markedly induced by
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
149
-
alanine
-, Q9W3Z3
pH 7.0, 50C, recombinant enzyme
0.01
-
glyoxylate
-
pH 7.4, 37C
0.0246
-
glyoxylate
-
pH 8.0, 30C, native enzyme
0.0336
-
glyoxylate
-
pH 8.0, 30C, recombinant enzyme
64
-
glyoxylate
-, Q9W3Z3
pH 7.0, 50C, recombinant enzyme
4
-
Hydroxypyruvate
-
pH 8.4-9.2, 15C
2.2
-
L-alanine
-
pH 8.4-9.2, 15C
8.1
-
L-serine
-
pH 8.4-9.2, 15C, Km increases with rising temperature, remains constant over the range 5-30C
0.21
-
pyruvate
-
pH 8.4-9.2, 15C
0.48
-
pyruvate
-
pH 7.4, 37C
1
-
pyruvate
-
pH 8.0, 37C, serine as amino donor
1.1
-
pyruvate
-
pH 8.0, 30C, native and recombinant enzyme
1.2
-
pyruvate
-
pH 8.0, 37C, serine as amino donor
1.6
-
pyruvate
-
pH 8.0, 37C, serine as amino donor
1.7
-
pyruvate
-
pH 8.0, 37C, serine as amino donor
38
-
pyruvate
-
pH 8.0, 30C
51
-
pyruvate
-, Q9W3Z3
pH 7.0, 50C, recombinant enzyme
0.39
-
serine
-
pH 8.0, 30C
8
-
serine
-
pH 8.0, 37C, pyruvate as amino acceptor
10
-
serine
-
pH 8.0, 37C, pyruvate as amino acceptor
16
-
serine
-
pH 8.0, 37C, pyruvate as amino acceptor
18
-
serine
-
pH 8.0, 37C, pyruvate as amino acceptor
256
-
serine
-, Q9W3Z3
pH 7.0, 50C, recombinant enzyme
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
131
-
alanine
-, Q9W3Z3
-
113
-
glyoxylate
-, Q9W3Z3
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2.02
-
-
alanine-hydroxypyruvate transamination
2.23
-
-
alanine-hydroxypyruvate transamination
3.47
-
-
serine-pyruvate aminotransferase activity
3.58
-
-
serine-pyruvate transamination
4.08
-
-
serine-pyruvate transamination
4.62
-
-
recombinant enzyme
5.41
-
-
recombinant enzyme
8.2
-
-
source leaf, age 10 days
8.3
-
-
source apical meristem, age 16 days
8.5
-
-
source apical meristem, age 15 days; source leaf, age 11 days
8.6
-
-
source apical meristem, age 13 days
8.8
-
-
source leaf, age 13 days
9.4
-
-
source apical meristem, age 10 days
9.5
-
-
source apical meristem, age 11 days
11.1
-
-
source leaf, age 16 days
13
-
-
serine-pyruvate transamination
13.1
-
-
source leaf, age 15 days
14
-
-
serine-pyruvate transamination
16.63
-
-
alanine-hydroxypyruvate transamination
25.7
-
-
histidine-pyruvate aminotransferase activity
31.38
-
-
alanine-hydroxypyruvate transamination
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.3
-
Q97VM5, -
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Donryu
-
-
-
Manually annotated by BRENDA team
-
induction by glucagon, leading to reduced formation of oxalate from L-hydroxyproline
Manually annotated by BRENDA team
Rattus norvegicus Donryu
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
;
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
;
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
73000
79000
-
sucrose density gradient centrifugation
73000
-
-
gel filtration
75000
-
-
sucrose density gradient centrifugation
80000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 38000, SDS-PAGE
dimer
-
2 * 43000, recombinant enzyme, SDS-PAGE
dimer
-
2 * 43000, SDS-PAGE
dimer
Rattus norvegicus Donryu
-
2 * 38000, SDS-PAGE
-
homodimer
-
2 * 44400, recombinant enzyme, SDS-PAGE
homodimer
Q97VM5, -
-
homodimer
-
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
1.8 A resolution. The structure of the Sulfolobus solfataricus enzyme is determined with an amino form of the cofactor pyridoxamine 5'-phosphate bound in the active site and in complex with gabaculine, an aminotransferase inhibitor. These structures show the changes in the enzyme active site during the course of the catalytic reaction
Q97VM5, -
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
70
-
-
heating of the purified enzyme for different lengths of time produces equivalent losses of both activities
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
enzyme recovered in the supernatant is resistant to proteinase K in presence or absence of Triton X-100, enzyme recovered in the precipitate is sensitive to the protease
-
precursor of mitochondrial enzyme is highly sensitive to proteinase K digestion, peroxisomal enzyme is fairly resistant to the protease
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 50 mM potassium phosphate buffer, pH 7.5, containing 0.1 M pyridoxal 5'-phosphate, may be stored for at least 4 weeks without loss of activity
-
0-6C, 50 mM potassium phosphate buffer, pH 7.5, containing 0.1 M pyridoxal 5'-phosphate, little loss with storage for at least 2 weeks
-
5C, acetone powder of fresh liver stored in a vacuum desiccator over aluminia retains its enzymatic activity for months
-
-20C, 50 mM potassium phosphate buffer, pH 7.5, containing 0.1 M pyridoxal 5'-phosphate, may be stored for at least 4 weeks without loss of activity
-
0-6C, 50 mM potassium phosphate buffer, pH 7.5, containing 0.1 M pyridoxal 5'-phosphate, little loss with storage for at least 2 weeks
-
-20C, 50 mM potassium phosphate buffer, pH 7.5, containing 0.1 M pyridoxal 5'-phosphate, may be stored for at least 4 weeks without loss of activity
-
0-6C, 50 mM potassium phosphate buffer, pH 7.5, containing 0.1 M pyridoxal 5'-phosphate, little loss with storage for at least 2 weeks
-
-20C, 50 mM potassium phosphate buffer, pH 7.5 may be stored for at least 6 weeks without loss of either activity
-
0-5C, little or none of either activity is lost when the enzyme is stored for 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant enzyme
-, Q9W3Z3
co-purification of serine:glyoxylate aminotransferase
-
isoenzyme 1 of histidine-pyruvate aminotransferase
-
mitochondrial and peroxisomal isoforms
-
native and recombinant enzyme
-
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in SF9 cells, using a insect/baculovirus expression system
-, Q9W3Z3
plasmid containing cDNA of human SPT cloned
-
binding sites identified by using a luciferase reporter assay with HepG2 cells, DNase I footprinting analysis and gel shift experiments
-
C-terminal SPT mutants constructed and examined in transfected COS-1 cells
-
cDNA cloned and expressed in Escherichia coli DH1
-
cloned and expressed in Escherichia coli DH5 and Escherichia coli ER1458
-
SPT/AGT gene is linked to the CAT gene and transiently expressed in human hepatoma-derived HepG2 cells
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
absence of human SPT/AGT or mistargeting of this enzyme causes primary hyperoxaluria type 1, an inborn error of glyoxylate metabolism characterized by increased oxalate production