Information on EC 2.6.1.50 - glutamine-scyllo-inositol transaminase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
2.6.1.50
-
RECOMMENDED NAME
GeneOntology No.
glutamine-scyllo-inositol transaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone = 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
streptomycin biosynthesis
-
-
Streptomycin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
L-glutamine:2,4,6/3,5-pentahydroxycyclohexanone aminotransferase
A pyridoxal-phosphate protein.
CAS REGISTRY NUMBER
COMMENTARY hide
9033-03-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Bacillus circulans SANK 72073
strain SANK 72073
-
-
Manually annotated by BRENDA team
ATCC 15835
-
-
Manually annotated by BRENDA team
no activity in Escherichia coli
host strain, free of enzymatic activity
-
-
Manually annotated by BRENDA team
no activity in Streptomyces lividans
66 strain 1326; 66 strain TK23, host strains, free of enzymatic activity
-
-
Manually annotated by BRENDA team
NRRL 1049b
-
-
Manually annotated by BRENDA team
strain NCIMB 8233, overexpression in Escherichia coli
SwissProt
Manually annotated by BRENDA team
ATCC 12628
-
-
Manually annotated by BRENDA team
JCM4433, nucleotide sequence deposited with the same accession number in EMBL/GenBank
SwissProt
Manually annotated by BRENDA team
ATCC 19756
-
-
Manually annotated by BRENDA team
ATCC 23265
-
-
Manually annotated by BRENDA team
JCM4923, nucleotide sequence deposited with the same accession number in EMBL/GenBank
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-scyllo-inosose + L-glutamine
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
show the reaction diagram
2-deoxy-scyllo-inosose + L-glutamine
2-deoxy-scyllo-inosamine + 2-oxoglutaramate
show the reaction diagram
-
-
-
-
?
2-deoxy-scyllo-inosose + L-glutamine
2-deoxy-scyllo-inosamine + 2-oxoglutarate
show the reaction diagram
2-deoxystreptamine + 2-ketoglutaramate
2-deoxy-keto-inosamine + L-glutamine
show the reaction diagram
-
-
-
r
2-deoxystreptamine + 2-oxoglutaramate
2-deoxy-scyllo-inositol + L-glutamine
show the reaction diagram
-
-
-
-
r
2-deoxystreptamine + keto-scyllo-inositol
2-deoxy-scyllo-inositol + amino-deoxy-keto-scyllo-inositol
show the reaction diagram
-
-
-
-
r
2-deoxystreptamine + pyruvate
2-keto-inosose + L-alanine
show the reaction diagram
aminodeoxy-scyllo-inositol + 2-oxoglutaramate
scyllo-inositol + L-glutamine
show the reaction diagram
-
-
-
-
r
aminodeoxy-scyllo-inositol + keto-scyllo-inositol
amino-scyllo-inositol + aminodeoxy-keto-scyllo-inositol
show the reaction diagram
-
-
-
-
r
L-glutamine + 1D-4-keto-myo-inositol
2-oxoglutaramate + amino-deoxy-1D-4-keto-myo-inositol
show the reaction diagram
-
-
-
-
r
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
show the reaction diagram
L-glutamine + 2-oxoadipate
2-oxoglutaramate + L-2-amino-1,6-hexandioate
show the reaction diagram
-
-
-
-
r
L-glutamine + 2-oxobutyrate
2-oxoglutaramate + 2-aminobutyrate
show the reaction diagram
-
-
-
-
r
L-glutamine + 2-oxocaproate
2-oxoglutaramate + L-norleucine
show the reaction diagram
-
-
-
-
r
L-glutamine + 2-oxoglutaramate
2-oxoglutaramate + L-glutamine
show the reaction diagram
-
-
-
-
r
L-glutamine + 2-oxoglutarate
2-oxoglutaramate + L-glutamate
show the reaction diagram
L-glutamine + 2-oxovalerate
2-oxoglutaramate + L-norvaline
show the reaction diagram
-
-
-
-
r
L-glutamine + DL-epi-inosose
2-oxoglutaramate + amino-deoxy-inosose
show the reaction diagram
-
-
-
-
r
L-glutamine + glyoxalate
2-oxoglutaramate + glycine
show the reaction diagram
-
-
-
-
r
L-glutamine + oxaloacetate
2-oxoglutaramate + L-aspartate
show the reaction diagram
-
-
-
-
r
L-glutamine + pyruvate
2-oxoglutaramate + L-alanine
show the reaction diagram
L-glutamine + scyllo-inosose
scyllo-inosamine + 2-oxoglutaramate
show the reaction diagram
-
-
spontaneously cyclization of 2-oxoglutaramate to 2-pyrrolidone-5-hydroxy-5-carboxylic acid
r
myo-inositol + diaminocyclitol + oxaloacetate
amino-deoxyinositol + ketoaminocyclitol + malate
show the reaction diagram
myo-inositol + glutamine + oxalacetate
amino-deoxyinositol + 2-oxoglutarate + malate + NH4+
show the reaction diagram
N3-methyl-2-deoxy-streptamine + inosose
aminodeoxyinositol + 1-keto-2-deoxy-3-methylaminodeoxy-scyllo-inositol
show the reaction diagram
N3-methyl-2-deoxy-streptamine + keto-scyllo-inositol
3-methylamino-2-deoxy-scyllo-inositol + 1-amino-1-deoxy-scyllo-inositol
show the reaction diagram
-
-
-
-
r
N3-methyl-2-deoxystreptamine + 2-oxoglutaramate
3-methylamino-2-deoxy-scyllo-inositol + L-glutamine
show the reaction diagram
-
-
-
-
r
scyllo-inosose + L-glutamine
scyllo-inosamine + 2-oxoglutaramate
show the reaction diagram
-
-
-
-
?
streptamine + 2-oxoglutaramate
amino-scyllo-inositol + L-glutamine
show the reaction diagram
-
-
-
-
r
streptamine + keto-scyllo-inositol
amino-scyllo-inositol + amino-deoxy-keto-scyllo-inositol
show the reaction diagram
-
-
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-deoxy-scyllo-inosose + L-glutamine
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
show the reaction diagram
-
tbmB catalyzes the second step of the DOS biosynthetic pathway during the biosynthesis of 2-deoxystreptamine, a subunit of tobramycin, in Streptomyces tenebrarius
-
-
?
L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone
2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithio-bis(2-nitrobenzoic acid)
-
reduces specific activity by 50%
p-hydroxymercuribenzoate
-
reduces specific activity by 50%
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45500
-
SDS-PAGE
64000 - 76000
-
gel filtration
95000 - 100000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 45000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
heat labile, loses all activity upon incubation for 20 min
55
-
relatively resistant to heating for 5 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity rapidly lost when dialyzed
-
pyridoxal 5'-phosphate or pyruvate stabilizes during dialysis
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stored at concentrations greater than 1mg/ml, stable for several months with little loss of activity
-
4°C, inactivated within 1 month
-
6°C, purified enzyme stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme, pJAW76-2, expressed in Streptomyces lividans
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; btrS homologous gene identified and sequenced
btrS gene identified and heterologously expressed
-
btrS homologous gene identified and sequenced
expressed heterologously in Escherichia coli
-
gene StsC identified, cloned by PCR and expressed in Escherichia coli BL21 and Streptomyces lividans
-
the gene is cloned into pIBR25, a Streptomyces expression vector and transformed into a non-aminoglycoside-producing host, Streptomyces venezuelae YJ003, for heterologous expression
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pharmacology
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