Information on EC 2.6.1.43 - aminolevulinate transaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.6.1.43
-
RECOMMENDED NAME
GeneOntology No.
aminolevulinate transaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
5-aminolevulinate + pyruvate = 4,5-dioxopentanoate + L-alanine
show the reaction diagram
A pyridoxal-phosphate protein
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Porphyrin and chlorophyll metabolism
-
SYSTEMATIC NAME
IUBMB Comments
5-aminolevulinate:pyruvate aminotransferase
A pyridoxal-phosphate protein.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4,5-dioxovalerate aminotransferase
-
-
-
-
4,5-dioxovaleric acid aminotransferase
-
-
-
-
4,5-dioxovaleric acid transaminase
-
-
-
-
4,5-dioxovaleric transaminase
-
-
-
-
5-aminolevulinic acid transaminase
-
-
-
-
alanine-dioxovalerate aminotransferase
-
-
-
-
alanine-gamma,delta-dioxovalerate aminotransferase
-
-
-
-
alanine:4,5-dioxovalerate aminotransferase
-
-
-
-
aminolevulinate aminotransferase
-
-
-
-
aminolevulinic acid transaminase
-
-
-
-
dioxovalerate transaminase
-
-
-
-
DOVA transaminase
-
-
-
-
EC 2.6.1.19
-
2-aminobutyrate aminotransferase and aminolevulinate transaminase activity reside in the same protein
EC 2.6.1.19
Rattus norvegicus Wistar
-
2-aminobutyrate aminotransferase and aminolevulinate transaminase activity reside in the same protein
-
EC 2.6.1.40
-
dimethylarginine-pyruvate aminotransferase and D-3-aminoisobutyrate pyruvate aminotransferase activities resides in the same protein
EC 2.6.1.40
-
dimethylarginine-pyruvate aminotransferase and D-3-aminoisobutyrate pyruvate aminotransferase activities resides in the same protein
EC 2.6.1.40
Rattus norvegicus Wistar
-
dimethylarginine-pyruvate aminotransferase and D-3-aminoisobutyrate pyruvate aminotransferase activities resides in the same protein
-
EC 2.6.1.44
-
alanine-glyoxylate transaminase and aminolevulinate transaminase activities reside in the same protein; identical with alanine-glyoxylate aminotransferase
EC 2.6.1.44
-
identical with alanine-glyoxylate aminotransferase
EC 2.6.1.44
Chlorella regularis S-50
-
identical with alanine-glyoxylate aminotransferase
-
EC 2.6.1.44
-
alanine-glyoxylate transaminase and aminolevulinate transaminase activities reside in the same protein
EC 2.6.1.44
-
identical with alanine-glyoxylate aminotransferase
EC 2.6.1.44
-
alanine-glyoxylate transaminase and aminolevulinate transaminase activities reside in the same protein
EC 2.6.1.44
Rattus norvegicus Wistar
-
alanine-glyoxylate transaminase and aminolevulinate transaminase activities reside in the same protein
-
gamma,delta-dioxovalerate aminotransferase
-
-
-
-
gamma,delta-dioxovaleric acid transaminase
-
-
-
-
L-alanine-4,5-dioxovalerate aminotransferase
-
-
-
-
L-alanine:4,5-dioxovaleric acid transaminase
-
-
-
-
L-alanine:dioxovalerate transaminase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9012-46-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Candida albicans 3100
3100
-
-
Manually annotated by BRENDA team
Chlorella regularis S-50
S-50
-
-
Manually annotated by BRENDA team
AG83, WHO nomenclature MHOM/IN/83/AG83, promastigote form
-
-
Manually annotated by BRENDA team
mouse
-
-
Manually annotated by BRENDA team
mouse; strain C57BL/6J and DBA/2J
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
strain C57BL/6J and DBA/2J
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae 3059 S288C
3059, S288C
-
-
Manually annotated by BRENDA team
Tulahuen strain, Tul 2 stock
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-alanine + 4,5-dioxopentanoate
5-aminolevulinate + malonic semialdehyde
show the reaction diagram
-
39% of the activity with L-alanine
-
-
ir
beta-alanine + 4,5-dioxopentanoate
5-aminolevulinate + malonic semialdehyde
show the reaction diagram
-
23% of the activity with L-alanine
-
-
ir
beta-alanine + 4,5-dioxopentanoate
5-aminolevulinate + malonic semialdehyde
show the reaction diagram
-
65.6% of the activity with L-alanine
-
-
ir
D-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
3% of the activity with L-alanine
-
-
ir
delta-amino-n-valerate + 4,5-dioxopentanoate
5-aminolevulinate + 5-oxo-n-pentanoate
show the reaction diagram
-
10% of the activity with L-alanine
-
-
ir
delta-amino-n-valerate + 4,5-dioxopentanoate
5-aminolevulinate + 5-oxo-n-pentanoate
show the reaction diagram
-
transaminate at 95% of the rate of alpha-alanine
-
-
ir
DL-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
89% of the activity with L-alanine
-
-
ir
epsilon-amino-n-caproate + 4,5-dioxopentanoate
5-aminolevulinate + 6-oxo-n-hexanoate
show the reaction diagram
-
transaminate at 103% of the rate of alpha-alanine
-
-
ir
epsilon-amino-n-caproate + 4,5-dioxopentanoate
5-aminolevulinate + 6-oxo-n-hexanoate
show the reaction diagram
-
34% of the activity with L-alanine
-
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
5-aminolevulinate + succinic semialdehyde
show the reaction diagram
-
transaminate at 122% of the rate of alpha-alanine
-
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
5-aminolevulinate + succinic semialdehyde
show the reaction diagram
-
55% of the activity with L-alanine
-
-
ir
gamma-amino-n-butyrate + 4,5-dioxopentanoate
5-aminolevulinate + succinic semialdehyde
show the reaction diagram
-
10% of the activity with L-alanine
-
-
ir
glycine + 4,5-dioxopentanoate
5-aminolevulinate + glyoxylate
show the reaction diagram
-
-
-
-
-
glycine + 4,5-dioxopentanoate
5-aminolevulinate + glyoxylate
show the reaction diagram
-
-
-
-
ir
glycine + 4,5-dioxopentanoate
5-aminolevulinate + glyoxylate
show the reaction diagram
-
less than 10% of the activity of alpha-alanine
-
-
ir
glycine + 4,5-dioxopentanoate
5-aminolevulinate + glyoxylate
show the reaction diagram
-
21% of the activity with L-alanine
-
-
ir
glycine + 4,5-dioxopentanoate
5-aminolevulinate + glyoxylate
show the reaction diagram
-
9% of the activity with L-alanine
-
-
ir
glycine + 4,5-dioxopentanoate
5-aminolevulinate + glyoxylate
show the reaction diagram
-
81.2% of the activity with L-alanine
-
-
ir
glycine + 4,5-dioxopentanoate
5-aminolevulinate + glyoxylate
show the reaction diagram
Candida albicans 3100
-
-
-
-
ir
glycine + 4,5-dioxopentanoate
5-aminolevulinate + glyoxylate
show the reaction diagram
Chlorella regularis S-50
-
21% of the activity with L-alanine
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
-
-
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
-
-
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
alternate route for delta-aminolevulinate formation
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
first committed step in heme synthesis
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
biosynthesis of porphyrin precursors
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
delta-aminolevulinate pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Candida albicans 3100
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Chlorella regularis S-50
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Rattus norvegicus Wistar
-
-, heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Rattus norvegicus Wistar
-
-, alternate route for delta-aminolevulinate formation, heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Mus musculus C57BL/6J
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Saccharomyces cerevisiae 3059 S288C
-
-
-
ir
L-arginine + 4,5-dioxopentanoate
5-aminolevulinate + 5-guanidino-2-oxopentanoate
show the reaction diagram
-
6.8% of the activity with L-alanine
-
-
ir
L-asparagine + 4,5-dioxopentanoate
5-aminolevulinate + 4-amino-2,4-dioxobutanoic acid
show the reaction diagram
-
74% of the activity with L-alanine
-
-
ir
L-aspartate + 4,5-dioxopentanoate
5-aminolevulinate + oxaloacetate
show the reaction diagram
-
39% of the activity with L-alanine
-
-
ir
L-aspartate + 4,5-dioxopentanoate
5-aminolevulinate + oxaloacetate
show the reaction diagram
-
67% of the activity with L-alanine
-
-
ir
L-glutamate + 4,5-dioxopentanoate
5-aminolevulinate + 2-oxoglutarate
show the reaction diagram
-
39% of the activity with L-alanine
-
-
ir
L-glutamate + 4,5-dioxopentanoate
5-aminolevulinate + 2-oxoglutarate
show the reaction diagram
-
37% of the activity with L-alanine
-
-
ir
L-glutamate + 4,5-dioxopentanoate
5-aminolevulinate + 2-oxoglutarate
show the reaction diagram
-
96% of the activity with L-alanine
-
-
ir
L-glutamate + 4,5-dioxopentanoate
5-aminolevulinate + 2-oxoglutarate
show the reaction diagram
-
43.8% of the activity with L-alanine
-
-
ir
L-glutamate + 4,5-dioxopentanoate
5-aminolevulinate + 2-oxoglutarate
show the reaction diagram
Candida albicans 3100
-
39% of the activity with L-alanine
-
-
ir
L-glutamate + 4,5-dioxopentanoate
5-aminolevulinate + 2-oxoglutarate
show the reaction diagram
Chlorella regularis S-50
-
37% of the activity with L-alanine
-
-
ir
L-glutamine + 4,5-dioxopentanoate
5-aminolevulinate + 2-oxoglutaramate
show the reaction diagram
-
74% of the activity with L-alanine
-
-
-
L-glutamine + 4,5-dioxopentanoate
5-aminolevulinate + 2-oxoglutaramate
show the reaction diagram
-
16% of the activity with L-alanine
-
-
-
L-histidine + 4,5-dioxopentanoate
5-aminolevulinate + 3-(1H-imidazol-4-yl)-2-oxopropanoate
show the reaction diagram
-
74% of the activity with L-alanine
-
-
ir
L-lysine + 4,5-dioxopentanoate
5-aminolevulinate + 6-amino-2-oxohexanoate
show the reaction diagram
-
9% of the activity with L-alanine
-
-
ir
L-lysine + 4,5-dioxopentanoate
5-aminolevulinate + 6-amino-2-oxohexanoate
show the reaction diagram
-
15% of the activity with L-alanine
-
-
ir
L-ornithine + 4,5-dioxopentanoate
5-aminolevulinate + 5-amino-2-oxopentanoate
show the reaction diagram
-
less than 10% of the activity of alpha-alanine
-
-
ir
L-ornithine + 4,5-dioxopentanoate
5-aminolevulinate + 5-amino-2-oxopentanoate
show the reaction diagram
-
14% of the activity with L-alanine
-
-
ir
L-ornithine + 4,5-dioxopentanoate
5-aminolevulinate + 5-amino-2-oxopentanoate
show the reaction diagram
-
6% of the activity with L-alanine
-
-
ir
L-phenylalanine + 4,5-dioxopentanoate
phenylpyruvate + 5-aminolevulinate
show the reaction diagram
-
71% of the activity with L-alanine
-
-
ir
L-phenylalanine + 4,5-dioxopentanoate
phenylpyruvate + 5-aminolevulinate
show the reaction diagram
-
90.6% of the activity with L-alanine
-
-
ir
L-serine + 4,5-dioxopentanoate
5-aminolevulinate + 3-hydroxy-2-oxopropanoate
show the reaction diagram
Chlorella regularis, Chlorella regularis S-50
-
33% of the activity with L-alanine
-
-
ir
additional information
?
-
-
ability of beta-alanine to replace alpha-alanine as amino group donor is lost during purification, alpha-amino-n-butyrate, alpha-aminoisobutyrate, beta-amino-n-butyrate and beta-aminoisobutyrate are inactive, serine, taurine, glutamine, asparagine, glutamic and aspartic acids, lysine and cysteine all have negligible activity
-
-
-
additional information
?
-
-
L-lysine, L-arginine, L-glutamate, L-methionine, L-histidine, glycine, L-phenylalanine, L-threonine, L-asparagine, L-serine, L-proline, L-valine, L-isoleucine, L-leucine, L-tyrosine, L-cysteine and L-tryptophan are all inert as amino donors, methylglyoxal is a poor substrate for the enzyme
-
-
-
additional information
?
-
-
does not use 2-oxoglutarate as amino acceptor
-
-
-
additional information
?
-
-
glutamate is no substrate, transamination of methylglyoxal yields aminoacetone, less than 1% efficient as amino acceptor as 4,5-dioxopentanoate
-
-
-
additional information
?
-
-
cysteine is no substrate
-
-
-
additional information
?
-
-
cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors
-
-
-
additional information
?
-
-
DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine
-
-
-
additional information
?
-
Candida albicans 3100
-
cannot utilize 2-oxoglutarate nor pyruvate as amino acceptors
-
-
-
additional information
?
-
Chlorella regularis S-50
-
does not use 2-oxoglutarate as amino acceptor
-
-
-
additional information
?
-
Rattus norvegicus Wistar
-
DL-alanine, D-alanine, ammonium sulfate, L-glutamate, glycine and phenylalanine cannot replace L-alanine
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
alternate route for delta-aminolevulinate formation
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
first committed step in heme synthesis
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
biosynthesis of porphyrin precursors
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
delta-aminolevulinate pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
-
heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Candida albicans 3100
-
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Chlorella regularis S-50
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Rattus norvegicus Wistar
-
heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Rattus norvegicus Wistar
-
alternate route for delta-aminolevulinate formation, heme biosynthetic pathway
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Mus musculus C57BL/6J
-
-
-
ir
L-alanine + 4,5-dioxopentanoate
5-aminolevulinate + pyruvate
show the reaction diagram
Saccharomyces cerevisiae 3059 S288C
-
-
-
ir
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
Km 0.0005 mM
pyridoxal 5'-phosphate
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-oxoglutarate
-
-
2-oxoglutarate
-
-
2-oxoglutarate
-
-
4,5-Dioxopentanoate
-
substrate inhibition
4,5-Dioxopentanoate
-
substrate inhibition
4,5-Dioxopentanoate
-
-
5-aminolevulinate
-
-
5-aminolevulinate
-
-
amino-oxyacetic acid
-
-
amino-oxyacetic acid
-
-
amino-oxyacetic acid
-
-
beta-Alanine
-
8% inhibition
beta-chloroalanine
-
-
beta-Iodopropionate
-
30% inhibition
cadaverine
-
14% inhibition
cysteine
-
90% inhibition
DL-dithiothreitol
-
-
ethyl acetoacetate
-
-
gabaculine
-
-
glyoxylic acid
-
competitive inhibition
glyoxylic acid
-
-
glyoxylic acid
-
-
glyoxylic acid
-
-
Hemin
-
uncompetitive inhibitor
Hemin
-
mitochondrial isozyme
hydroxylamine
-
-
iodoacetate
-
30% inhibition
Isonicotinic acid hydrazide
-
-
Isonicotinic acid hydrazide
-
-
lysine
-
20% inhibition
Methylglyoxal
-
-
Methylglyoxal
-
-
Methylglyoxal
-
-
Methylglyoxal
-
-
ornithine
-
20% inhibition
oxaloacetic acid
-
-
p-chloromercuribenzoate
-
78% inhibition
p-chloromercuribenzoate
-
-
p-chloromercuric benzene sulfonate
-
-
potassium cyanide
-
-
protoporphyrin
-
-
pyruvate
-
-
Semicarbazide
-
-
Succinate semialdehyde
-
-
Succinate semialdehyde
-
-
Thioglycollate
-
74% inhibition
Tris-HCl
-
-
Methylglyoxal
-
-
additional information
-
iodoacetamide and chloroacetamide are not inhibitors
-
additional information
-
EDTA, N-ethylmaleimide. iodoacetic acid, cysteine and beta-mercaptoethanol are no inhibitors
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Hemin
-
cytoplasmic isozyme, stimulates to 150% of the control
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0105
-
4,5-Dioxopentanoate
-
pH 7.2, 60C
0.06
-
4,5-Dioxopentanoate
-
pH 7.0
0.12
-
4,5-Dioxopentanoate
-
pH 8.0, 37C
0.22
-
4,5-Dioxopentanoate
-
-
0.24
-
4,5-Dioxopentanoate
-
pH 6.9, 37C
0.25
-
4,5-Dioxopentanoate
-
pH 7.6, 37C
0.26
-
4,5-Dioxopentanoate
-
pH 6.9, 37C
0.28
-
4,5-Dioxopentanoate
-
pH 6.9, 37C
0.3
-
4,5-Dioxopentanoate
-
pH 7.0, 37C
0.4
-
4,5-Dioxopentanoate
-
pH 7.0, 37C
0.71
-
4,5-Dioxopentanoate
-
-
0.75
-
4,5-Dioxopentanoate
-
pH 8.0, 37C
1.44
-
4,5-Dioxopentanoate
-
pH 7.0, 65C
1.8
-
4,5-Dioxopentanoate
-
-
0.67
-
glyoxylate
-
pH 7.0, 37C
1.3
-
glyoxylate
-
pH 8.0, 37C
0.00028
-
L-alanine
-
pH 7.2, 60C
0.4
-
L-alanine
-
pH 7.0
0.97
-
L-alanine
-
-
1.7
-
L-alanine
-
-
1.7
-
L-alanine
-
pH 8.0, 37C
1.96
-
L-alanine
-
pH 6.9, 37C
2.5
-
L-alanine
-
pH 7.0, 37C, 4,5-dioxopentanoate as amino acceptor
2.9
-
L-alanine
-
pH 7.6, 37C
3
-
L-alanine
-
pH 8.0, 37C, glyoxylate as amino acceptor
3.3
-
L-alanine
-
pH 6.9, 37C
3.44
-
L-alanine
-
-
3.5
-
L-alanine
-
pH 8.0, 37C
3.7
-
L-alanine
-
pH 6.9, 37C
4
-
L-alanine
-
pH 7.0, 37C, glyoxylate as amino acceptor
8
-
L-alanine
-
pH 7.0, 37C
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.5
-
glyoxylate
-
pH 8.0, 37C
30
-
Hemin
-
different concentrations of alanine at fixed 4,5-dioxopentanoate concentration
42
-
Hemin
-
different concentrations of 4,5-dioxopentanoate concentration at fixed alanine concentration
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0266
-
-
-
0.16
-
-
-
0.186
-
-
-
0.22
-
-
cytosolic isozyme
0.753
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.6
-
-
cytoplasmic isozyme
6.9
-
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
65
-
-
cytoplasmic isozyme
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
80
-
3fold increase of activity from 37C to 60C
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.7
4.8
-
isoelectric focusing using Phast GEl IEF 3-9
4.8
-
-
isoelectrofocusing column 110 ml and Pharmalyte gradient pH 3-10 at 4C for 45 h
5
-
-
isoelectric focusing analysis, 2-dimensional gel electrophoresis
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Mus musculus C57BL/6J
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
;
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
cytosol contains minimal enzymic activity
Manually annotated by BRENDA team
-
different isozymes in cytosol and mitochondrion
Manually annotated by BRENDA team
Candida albicans 3100
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
different isozymes in cytosol and mitochondrion
-
Manually annotated by BRENDA team
-
mitochondrial matrix
Manually annotated by BRENDA team
-
different isozymes in cytosol and mitochondrion
Manually annotated by BRENDA team
-
mitochondrial matrix
Manually annotated by BRENDA team
Candida albicans 3100
-
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
mitochondrial matrix
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
-
different isozymes in cytosol and mitochondrion; mitochondrial matrix
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60260
-
-
gel filtration
63000
-
-
SDS-PAGE
72000
-
-
sedimentation equilibrium centrifugation, potassium phosphate buffer containing 4 M NaCl
78000
-
-
sedimentation equilibrium centrifugation, potassium phosphate buffer
82000
-
-
gel filtration
111000
-
-
gel filtration
123000
-
-
-
126000
-
-
gel filtration
146000
-
-
SDS-PAGE
168000
-
-
gel filtration
190000
-
-
gel filtration
210000
-
-
mitochondrial isozyme, gel filtration
210000
-
-
gel filtration, gradient polyacrylamide gel electrophoresis
225000
-
-
gel filtration
225000
-
-
gel filtration, Sepharose 6B
232000
-
-
gel filtration
238000
-
-
gel filtration
240000
-
-
gel filtration
240000
-
-
-
245000
-
-
sucrose density gradient centrifugation
260000
-
-
cytoplasmic isozyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 55500, SDS-PAGE
dimer
-
2 * 68000, SDS-PAGE
dimer
Chlorella regularis S-50
-
2 * 68000, SDS-PAGE
-
hexamer
-
6 * 37000, SDS-PAGE
hexamer
-
6 * 41000
hexamer
Rattus norvegicus Wistar
-
6 * 41000
-
monomer
-
1 * 57544, SDS-PAGE
monomer
-
1 * 59000, SDS-PAGE
monomer
Candida albicans 3100
-
1 * 57544, SDS-PAGE
-
monomer
Saccharomyces cerevisiae 3059 S288C
-
1 * 59000, SDS-PAGE
-
tetramer
-
4 * 58000, SDS-PAGE
tetramer
-
4 * 48000, SDS-PAGE
tetramer
-
4 * 42000, SDS-PAGE
tetramer
-
4 * 50000, mitochondrial isozyme, SDS-PAGE; 4 * 67000, cytoplasmic isozyme, homopolymer, SDS-PAGE
tetramer
-
4 * 50000, mitochondrial isozyme, SDS-PAGE
tetramer
Rattus norvegicus Wistar
-
4 * 50000, mitochondrial isozyme, SDS-PAGE; 4 * 50000, mitochondrial isozyme, SDS-PAGE; 4 * 67000, cytoplasmic isozyme, homopolymer, SDS-PAGE
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
crystallized as bright yellow needles
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
55
65
-
retains more than 90% maximum activity through 55-65C, beyond 65C activity decreases sharply
55
-
-
retains 75% activity after 1 h incubation
60
-
-
10 min, about 65% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
freezing and thawing does not affect activity
-
repeated freezing and thawing inactivates the enzyme, stability of the enzyme is enhanced by presence of 5% glycerol
-
stability is improved by 10-15% glycerol
-
proteolytic hydrolysis of the cytosolic isozyme generates an enzymatically more active form
-
purified enzyme is stable in 50 mM potassium buffer, pH 6.9, containing 10% glycerol, freezing and thawing does not affect activity significantly
-
repeated freezing and thawing causes inactivation
-
glutathione or beta-mercaptoethanol restores activity of aged enzyme preparations, optimally at 1.0 mM
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, purified enzyme in 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, may be stored for at least 8 weeks without loss of either activity
-
0-5C, 50 mM potassium phosphate buffer, pH 7.5, 10% glycerol, stored for 2 weeks, little or none of each activity is lost
-
4C, stored in ice for 3 months without any appreciable loss of enzyme activity
-
4C, 10-15% glycerol, 20% loss of activity after 5 days
-
4C, unstable
-
-15C, stable for 3 months
-
-20C, 5 mM potassium phosphate buffer, pH 7.6, 10% glycerol
-
-20C, can be stored for nearly 1 year without significant loss of enzyme activity
-
-20C, remains stable for at least 1 month
-
-70C, stable for at least 2-3 months
-
4C, can be stored for at least 1 week without any significant loss of activity
-
-15C, stable for several months
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
immunological difference between the enzyme from mammalian source and this enzyme presents a potential target for chemotherapy, development of treatment for Leishmaniasis, a major parasitic disease in humans and opportunistic infections in patients with AIDS, an inhibitor specific for the leishmanial enzyme may be used as a therapeutic agent
medicine
-
target for chemotherapy against Chagas disease or American trypanosomiasis