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Information on EC 2.6.1.30 - pyridoxamine-pyruvate transaminase and Organism(s) Mesorhizobium japonicum and UniProt Accession Q988B8

for references in articles please use BRENDA:EC2.6.1.30
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EC Tree
     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.30 pyridoxamine-pyruvate transaminase
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This record set is specific for:
Mesorhizobium japonicum
UNIPROT: Q988B8 not found.
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Word Map
The taxonomic range for the selected organisms is: Mesorhizobium japonicum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
pyridoxamine-pyruvate aminotransferase, pyridoxamine-pyruvate transaminase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminotransferase, pyridoxamine-pyruvate
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pyridoxamine-pyruvate aminotransferase
pyridoxamine-pyruvic transaminase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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SYSTEMATIC NAME
IUBMB Comments
pyridoxamine:pyruvate aminotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9023-38-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pyridoxamine + pyruvate
pyridoxal + L-alanine
show the reaction diagram
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyridoxamine + pyruvate
pyridoxal + L-alanine
show the reaction diagram
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-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
in vivo, recombinant enzyme in Rhodococcus erythropolis cells
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
over 50% of maximal activity within this range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 35
in vivo, recombinant enzyme in Rhodococcus erythropolis cells
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 40
over 50% of maximal activity within this range
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Mesorhizobium loti strain MAFF 303099
UniProt
Manually annotated by BRENDA team
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 42000, recombinant soluble enzyme, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pyridoxamine is produced from pyridoxine by bioconversion using a Rhodococcus expression system. Approximately 450 mM pyridoxal are produced from 500 mM pyridoxine using recombinant Rhodococcus erythropolis expressing the pyridoxine 4-oxidase gene derived from Mesorhizobium loti. In the bioconversion of pyridoxal to pyridoxamine using recombinant Rhodococcus erythropolis expressing the pyridoxamine-pyruvate aminotransferase gene derived from Mesorhizobium loti, the bioconversion rate is approximately 80% under the same conditions as pyridoxal production. Finally, in the bioconversion of pyridoxine to pyridoxamine through pyridoxal using recombinant Rhodococcus erythropolis coexpressing the genes for pyridoxine 4-oxidase and pyridoxamine-pyruvate aminotransferase, the bioconversion rate is approximately 75%. Reactor bioconversion using the Rhodococcus expression system, method evaluation and optimization, overview
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ppaT, functional recombinant expression of soluble enzyme in Rhodococcus erythropolis strain JCM3191, co-expression with pyridoxine 4-oxidase (PNO) from Mesorhizobium loti from gene pno
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
pyridoxamine production by bioconversion is generally preferable for environmental and energetic aspects compared to chemical synthesis. Pyridoxamine is produced from pyridoxine, a readily and economically available starting material, by bioconversion using a Rhodococcus expression system
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamamura, E.T.
Bioconversion of pyridoxine to pyridoxamine through pyridoxal using a Rhodococcus expression system
J. Biosci. Bioeng.
127
79-84
2019
Mesorhizobium japonicum (Q988B8), Mesorhizobium japonicum CECT 9101 (Q988B8), Mesorhizobium japonicum LMG 29417 (Q988B8)
Manually annotated by BRENDA team