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Information on EC 2.6.1.27 - tryptophan transaminase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9S7N2

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EC Tree
     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.27 tryptophan transaminase
IUBMB Comments
A pyridoxal-phosphate protein. Also acts on 5-hydroxytryptophan and, to a lesser extent, on the phenyl amino acids.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9S7N2
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
tryptophan aminotransferase, ostar1, tryptophan aminotransferase of arabidopsis 1, l-tryptophan aminotransferase, tryptophan transaminase, vanishing tassel2, protein fib, lmtam1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L-tryptophan aminotransferase
-
L-tryptophan-pyruvate aminotransferase 1
UniProt
tryptophan aminotransferase of Arabidopsis 1
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5-hydroxytryptophan-ketoglutaric transaminase
-
-
-
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aminotransferase, tryptophan
-
-
-
-
hydroxytryptophan aminotransferase
-
-
-
-
L-phenylalanine-2-oxoglutarate aminotransferase
-
-
-
-
L-tryptophan aminotransferase
-
-
-
-
L-tryptophan transaminase
-
-
-
-
tryptophan aminotransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
L-tryptophan:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Also acts on 5-hydroxytryptophan and, to a lesser extent, on the phenyl amino acids.
CAS REGISTRY NUMBER
COMMENTARY hide
9022-98-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-tryptophan + 2-oxoglutarate
(indol-3-yl)pyruvate + L-glutamate
show the reaction diagram
-
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-tryptophan + 2-oxoglutarate
(indol-3-yl)pyruvate + L-glutamate
show the reaction diagram
-
-
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(aminooxy)-3-(naphthalen-2-yl)propanoic acid
KOK1169/AONP, the compound is an improved, that is more specific for TAA1 than for other enzymes, such as phenylalanine ammonia-lyase. KOK1169 inhibits TAA1 in a competitive manner
aminooxy-naphthylpropionic acid
AOPP
L-alpha-(2-aminoethoxyvinyl)glycine
AVG, a typical ACS inhibitor
L-kynurenine
L-kynurenine
competitively inhibits TAA1/TAR activity, and Kyn treatment mimicks the loss of TAA1/TAR functions
additional information
aminooxy-naphthylpropionic acid and derivatives are inhibitors of auxin biosynthesis targeting L-tryptophan aminotransferase, structure-activity relationships, overview. Synthesis of potential inhibitors and docking simulation analysis including KOK1169/AONP. The developed compounds constitute a class of inhibitors of TAA1, designated as pyruvamine
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000768
2-(aminooxy)-3-(naphthalen-2-yl)propanoic acid
pH 8.5, 35°C, recombinant enzyme
0.0003504
aminooxy-naphthylpropionic acid
pH 8.5, 35°C, recombinant enzyme
0.0086
L-alpha-(2-aminoethoxyvinyl)glycine
pH 8.5, 35°C, recombinant enzyme
0.01152
L-kynurenine
pH and temperature not specified in the publication
0.01152
L-kynurenine
pH and temperature not specified in the publication
additional information
additional information
inhibition kinetic analysis
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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phenotypes observed in taa mutants, including root resistance to ethylene treatment, altered shade avoidance responses, and root resistance to naphthylphthalamic acid treatments, are phenocopied by inactivating certain combinations of YUC genes. Synergistic effects between yuc and known auxin mutants are observed when TAA genes are inactivated in the known auxin mutant backgrounds. Overexpression of YUC1 in taa1/sav3 partially rescues the shade avoidance defects. Using a genetic analysis it is shown that YUC and TAA genes are in the same auxin biosynthesis pathway, with YUCs downstream of TAAs
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TAA1_ARATH
391
0
44801
Swiss-Prot
other Location (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
recombinant enzyme expression in Escherichia coli
expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
He, W.; Brumos, J.; Li, H.; Ji, Y.; Ke, M.; Gong, X.; Zeng, Q.; Li, W.; Zhang, X.; An, F.; Wen, X.; Li, P.; Chu, J.; Sun, X.; Yan, C.; Yan, N.; Xie, D.Y.; Raikhel, N.; Yang, Z.; Stepanova, A.N.; Alonso, J.M.; Guo, H.
A small-molecule screen identifies L-kynurenine as a competitive inhibitor of TAA1/TAR activity in ethylene-directed auxin biosynthesis and root growth in Arabidopsis
Plant Cell
23
3944-3960
2011
Arabidopsis thaliana (Q9LR29), Arabidopsis thaliana (Q9S7N2), Arabidopsis thaliana
Manually annotated by BRENDA team
Won, C.; Shen, X.; Mashiguchi, K.; Zheng, Z.; Dai, X.; Cheng, Y.; Kasahara, H.; Kamiya, Y.; Chory, J.; Zhao, Y.
Conversion of tryptophan to indole-3-acetic acid by TRYPTOPHAN AMINOTRANSFERASES OF ARABIDOPSIS and YUCCAs in Arabidopsis
Proc. Natl. Acad. Sci. USA
108
18518-18523
2011
Arabidopsis thaliana
Manually annotated by BRENDA team
Narukawa-Nara, M.; Nakamura, A.; Kikuzato, K.; Kakei, Y.; Sato, A.; Mitani, Y.; Yamasaki-Kokudo, Y.; Ishii, T.; Hayashi, K.; Asami, T.; Ogura, T.; Yoshida, S.; Fujioka, S.; Kamakura, T.; Kawatsu, T.; Tachikawa, M.; Soeno, K.; Shimada, Y.
Aminooxy-naphthylpropionic acid and its derivatives are inhibitors of auxin biosynthesis targeting l-tryptophan aminotransferase structure-activity relationships
Plant J.
87
245-257
2016
Arabidopsis thaliana (Q9S7N2), Arabidopsis thaliana Col-0 (Q9S7N2)
Manually annotated by BRENDA team