Information on EC 2.6.1.24 - diiodotyrosine transaminase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY
2.6.1.24
-
RECOMMENDED NAME
GeneOntology No.
diiodotyrosine transaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3,5-diiodo-L-tyrosine + 2-oxoglutarate = 4-hydroxy-3,5-diiodophenylpyruvate + L-glutamate
show the reaction diagram
also acts on 3,5-dichloro-, 3,5-dibromo- and 3-iodo-L-tyrosine, thyroxine and triiodothyronine
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
amino group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3,5-diiodo-L-tyrosine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Also acts on 3,5-dichloro-, 3,5-dibromo- and 3-iodo-L-tyrosine, thyroxine and triiodothyronine.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminotransferase, diiodotyrosine
-
-
-
-
diiodotyrosine aminotransferase
-
-
-
-
halogenated tyrosine aminotransferase
-
-
-
-
halogenated tyrosine transaminase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9033-18-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,5-dibromo-L-tyrosine + 2-oxoglutarate
3,5-dibromo-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
-
at 127% the rate of 3,5-diiodo-L-tyrosine
-
-
?
3,5-dichloro-L-tyrosine + 2-oxoglutarate
3,5-dichloro-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
-
at 110% the rate of 3,5-diiodo-L-tyrosine
-
-
?
3,5-diiodo-L-tyrosine + 2-oxoglutarate
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
-
-, specific for 2-oxoglutarate as amino acceptor
-
?
3,5-diiodo-L-tyrosine + oxaloacetate
3,5-diiodo-4-hydroxyphenylpyruvate + L-aspartate
show the reaction diagram
-
30% substitution of 2-oxoglutarate by oxaloacetate as amino acceptor
-
?
3-(4-fluorophenyl)alanine + 2-oxoglutarate
(4-fluorophenyl)pyruvate + L-glutamate
show the reaction diagram
-
i.e. p-fluoro-DL-phenylalanine, at 3.1% the rate of 3,5-diiodo-L-tyrosine
-
-
?
3-iodo-L-tyrosine + 2-oxoglutarate
3-iodo-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
-
at 76% the rate of 3,5-diiodo-L-tyrosine
-
-
?
L-phenylalanine + 2-oxoglutarate
phenylpyruvate + L-glutamate
show the reaction diagram
-
at 3.9% the rate of 3,5-diiodo-L-tyrosine
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-
?
L-tryptophan + 2-oxoglutarate
3-indole-2-oxopropanoate + L-glutamate
show the reaction diagram
-
at 3.6% the rate of 3,5-diiodo-L-tyrosine
-
-
?
L-tyrosine + 2-oxoglutarate
(4-hydroxyphenyl)pyruvate + L-glutamate
show the reaction diagram
-
at 3.9% the rate of 3,5-diiodo-L-tyrosine
-
-
?
additional information
?
-
-
substrate specificity, no activity with: D-diiodotyrosine, tyrosine analogues with nitro, amino or hydroxy substituents in position 3 or 3 and 5, no activity with pyruvate as amino acceptor
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,5-diiodo-L-tyrosine + 2-oxoglutarate
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
required
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3,5-Diiodo-4-hydroxyphenyl-DL-lactic acid
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-
3,5-Diiodo-4-hydroxyphenylacetate
-
strong inhibition, mixed type
4-chloromercuribenzoate
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-
4-hydroxyphenyl-DL-lactic acid
-
weak
4-hydroxyphenylacetic acid
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-
o-Iodosobenzoate
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
3.4
3.7
3,5-Dibromo-L-tyrosine
-
pH 6.5, 37C
6.1
-
3,5-dichloro-L-tyrosine
-
pH 6.-L-7, 37C
1.1
1.3
3,5-diiodo-L-tyrosine
-
pH 6.2, 37C
6.5
-
3-iodo-L-tyrosine
-
pH 7.4, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.53
-
-
partially purified enzyme
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
-
-
with substrate diiodo-L-tyrosine
6.5
-
-
with substrate dibromo-L-tyrosine, dichloro-L-tyrosine
7
7.5
-
with substrate monoiodo-L-tyrosine
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
80000
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-
gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
0C, 50 mM sodium phosphate buffer, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.15 M NaCl, approximately 4 mg protein/ml, 40% loss of activity after 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE