Information on EC 2.6.1.24 - diiodotyrosine transaminase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY
2.6.1.24
-
RECOMMENDED NAME
GeneOntology No.
diiodotyrosine transaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3,5-diiodo-L-tyrosine + 2-oxoglutarate = 4-hydroxy-3,5-diiodophenylpyruvate + L-glutamate
show the reaction diagram
also acts on 3,5-dichloro-, 3,5-dibromo- and 3-iodo-L-tyrosine, thyroxine and triiodothyronine
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
amino group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
3,5-diiodo-L-tyrosine:2-oxoglutarate aminotransferase
A pyridoxal-phosphate protein. Also acts on 3,5-dichloro-, 3,5-dibromo- and 3-iodo-L-tyrosine, thyroxine and triiodothyronine.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminotransferase, diiodotyrosine
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-
-
-
diiodotyrosine aminotransferase
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-
-
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halogenated tyrosine aminotransferase
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-
-
-
halogenated tyrosine transaminase
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-
-
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CAS REGISTRY NUMBER
COMMENTARY
9033-18-5
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,5-dibromo-L-tyrosine + 2-oxoglutarate
3,5-dibromo-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
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at 127% the rate of 3,5-diiodo-L-tyrosine
-
-
?
3,5-dichloro-L-tyrosine + 2-oxoglutarate
3,5-dichloro-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
-
at 110% the rate of 3,5-diiodo-L-tyrosine
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-
?
3,5-diiodo-L-tyrosine + 2-oxoglutarate
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
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specific for 2-oxoglutarate as amino acceptor
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?
3,5-diiodo-L-tyrosine + oxaloacetate
3,5-diiodo-4-hydroxyphenylpyruvate + L-aspartate
show the reaction diagram
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30% substitution of 2-oxoglutarate by oxaloacetate as amino acceptor
-
?
3-(4-fluorophenyl)alanine + 2-oxoglutarate
(4-fluorophenyl)pyruvate + L-glutamate
show the reaction diagram
-
i.e. p-fluoro-DL-phenylalanine, at 3.1% the rate of 3,5-diiodo-L-tyrosine
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-
?
3-iodo-L-tyrosine + 2-oxoglutarate
3-iodo-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
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at 76% the rate of 3,5-diiodo-L-tyrosine
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-
?
L-phenylalanine + 2-oxoglutarate
phenylpyruvate + L-glutamate
show the reaction diagram
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at 3.9% the rate of 3,5-diiodo-L-tyrosine
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-
?
L-tryptophan + 2-oxoglutarate
3-indole-2-oxopropanoate + L-glutamate
show the reaction diagram
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at 3.6% the rate of 3,5-diiodo-L-tyrosine
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-
?
L-tyrosine + 2-oxoglutarate
(4-hydroxyphenyl)pyruvate + L-glutamate
show the reaction diagram
-
at 3.9% the rate of 3,5-diiodo-L-tyrosine
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-
?
additional information
?
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substrate specificity, no activity with: D-diiodotyrosine, tyrosine analogues with nitro, amino or hydroxy substituents in position 3 or 3 and 5, no activity with pyruvate as amino acceptor
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,5-diiodo-L-tyrosine + 2-oxoglutarate
3,5-diiodo-4-hydroxyphenylpyruvate + L-glutamate
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
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required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3,5-Diiodo-4-hydroxyphenyl-DL-lactic acid
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3,5-Diiodo-4-hydroxyphenylacetate
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strong inhibition, mixed type
4-chloromercuribenzoate
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4-hydroxyphenyl-DL-lactic acid
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weak
4-hydroxyphenylacetic acid
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o-Iodosobenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.4 - 3.7
3,5-Dibromo-L-tyrosine
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pH 6.5, 37C
6.1
3,5-dichloro-L-tyrosine
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pH 6.-L-7, 37C
1.1 - 1.3
3,5-diiodo-L-tyrosine
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pH 6.2, 37C
6.5
3-iodo-L-tyrosine
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pH 7.4, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.53
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partially purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6
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with substrate diiodo-L-tyrosine
6.5
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with substrate dibromo-L-tyrosine, dichloro-L-tyrosine
7 - 7.5
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with substrate monoiodo-L-tyrosine
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
80000
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gel filtration
639936
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0C, 50 mM sodium phosphate buffer, 1 mM EDTA, 1 mM 2-mercaptoethanol, 0.15 M NaCl, approximately 4 mg protein/ml, 40% loss of activity after 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE