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Information on EC 2.6.1.19 - 4-aminobutyrate-2-oxoglutarate transaminase and Organism(s) Escherichia coli and UniProt Accession P22256

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EC Tree
     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.19 4-aminobutyrate-2-oxoglutarate transaminase
IUBMB Comments
Requires pyridoxal phosphate. Some preparations also act on beta-alanine, 5-aminopentanoate and (R,S)-3-amino-2-methylpropanoate. cf. EC 2.6.1.120, beta-alanine---2-oxoglutarate transaminase.
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This record set is specific for:
Escherichia coli
UNIPROT: P22256
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
gaba-t, gaba transaminase, gaba-transaminase, gaba-at, 4-aminobutyrate aminotransferase, gaba aminotransferase, gamma-aminobutyric acid transaminase, gabat, gamma-aminobutyric acid aminotransferase, gamma-aminobutyrate transaminase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GABA transaminase
-
gamma-aminobutyric acid transaminase
-
4-aminobutyrate aminotransferase
-
-
-
-
4-aminobutyrate-2-ketoglutarate aminotransferase
-
-
-
-
4-aminobutyrate-2-oxoglutarate aminotransferase
-
-
-
-
4-aminobutyrate-2-oxoglutarate transaminase
-
-
-
-
4-aminobutyric acid 2-ketoglutaric acid aminotransferase
-
-
-
-
4-aminobutyric acid aminotransferase
-
-
-
-
aminobutyrate aminotransferase
-
-
-
-
aminobutyrate transaminase
-
-
-
-
aminotransferase, aminobutyrate
-
-
-
-
beta-alanine aminotransferase
-
-
-
-
beta-alanine transaminase
-
-
-
-
beta-alanine-oxoglutarate aminotransferase
-
-
-
-
beta-alanine-oxoglutarate transaminase
-
-
-
-
GABA aminotransferase
-
-
-
-
GABA transaminase
-
-
-
-
GABA transferase
-
-
-
-
GABA-2-oxoglutarate aminotransferase
-
-
-
-
GABA-2-oxoglutarate transaminase
-
-
-
-
GABA-alpha-ketoglutarate aminotransferase
-
-
-
-
GABA-alpha-ketoglutarate transaminase
-
-
-
-
GABA-alpha-ketoglutaric acid transaminase
-
-
-
-
GABA-alpha-oxoglutarate aminotransferase
-
-
-
-
GABA-oxoglutarate aminotransferase
-
-
-
-
GABA-oxoglutarate transaminase
-
-
-
-
gamma-aminobutyrate aminotransaminase
-
-
-
-
gamma-aminobutyrate transaminase
-
-
-
-
gamma-aminobutyrate-alpha-ketoglutarate aminotransferase
-
-
-
-
gamma-aminobutyrate-alpha-ketoglutarate transaminase
-
-
-
-
gamma-aminobutyrate:alpha-oxoglutarate aminotransferase
-
-
-
-
gamma-aminobutyric acid aminotransferase
-
-
-
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gamma-aminobutyric acid pyruvate transaminase
-
-
-
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gamma-aminobutyric acid transaminase
-
-
-
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gamma-aminobutyric acid-2-oxoglutarate transaminase
-
-
-
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gamma-aminobutyric acid-alpha-ketoglutarate transaminase
-
-
-
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gamma-aminobutyric acid-alpha-ketoglutaric acid aminotransferase
-
-
-
-
gamma-aminobutyric transaminase
-
-
-
-
glutamate-succinic semialdehyde transaminase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
show the reaction diagram
active site comparison with pig enzyme and with dialkylglycine decarboxylase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amino group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
4-aminobutanoate:2-oxoglutarate aminotransferase
Requires pyridoxal phosphate. Some preparations also act on beta-alanine, 5-aminopentanoate and (R,S)-3-amino-2-methylpropanoate. cf. EC 2.6.1.120, beta-alanine---2-oxoglutarate transaminase.
CAS REGISTRY NUMBER
COMMENTARY hide
9037-67-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-aminobutanoate + 2-oxoglutarate
4-oxobutanoate + L-glutamate
show the reaction diagram
-
-
-
?
4-aminobutanoate + 2-oxoglutarate
succinate semialdehyde + L-glutamate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-aminobutanoate + 2-oxoglutarate
succinate semialdehyde + L-glutamate
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoglutarate
substrate inhibition
Aminooxyacetate
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0021 - 46
2-oxoglutarate
4.7 - 146
4-aminobutanoate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00023 - 47.4
4-aminobutanoate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.24 - 1.13
Aminooxyacetate
19 - 233
Glutarate
3.9 - 780
Maleate
27 - 720
succinate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
GabT activity of the recombinant Escherichia coli enzyme expressed in Agrobacterium tumefaciens enhances the T-DNA transfer ability of Agrobacterium tumefaciens
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with inhibitor aminooxyacetate
mutant I50Q, V241A, E211S
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E211S
crystallization data, decrease in kcat, decrease in Km-value for 2-oxoglutarate
E211S/I50G
drastic decrease in kcat-value
E211S/I50G/C77K
10fold increase in decarboxylation activity
E211S/I50G/C77R
drastic decrease in kcat-value
E211S/I50H/V80D
10fold increase in decarboxylation activity, change of reaction specificity to that of a decarboxylase
E211S/I50H/V80T
decrease in kcat, decrease in Km-value for 2-oxoglutarate
E211S/I50N/V80D
drastic decrease in kcat-value
E211S/I50N/V80T
drastic decrease in kcat-value
E211S/I50Q/G295Y/V241A
drastic decrease in kcat-value
I50Q
crystallization data, decrease in kcat, increase in Km-values
I50Q/G295Y
decrease in kcat, decrease in Km-value for 2-oxoglutarate
V241A
crystallization data, decrease in kcat, decrease in Km-value for 2-oxoglutarate
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene gabT, recombinant expression in Agrobacterium tumefaciens strain C58
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology
Agrobacterium tumefaciens is used to mediate inter-kingdom DNA transfer in plant genetic engineering. Gamma-aminobutyric acid (GABA) is a negative factor in the Agrobacterium-plant interaction, because it inhibits the DNA transfer. Generation of an Agrobacterium tumefaciens strain expressing the Escherichia coli gene gabT, which introduces GABA transaminase activity and the ability to degrade GABA, is achieved to circumvent the inhibitory effect of GABA
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Liu, W.; Peterson, P.E.; Carter, R.J.; Zhou, X.; Langston, J.A.; Fisher, A.J.; Toney, M.D.
Crystal structures of unbound and aminooxyacetate-bound Escherichia coli gamma-aminobutyrate aminotransferase
Biochemistry
43
10896-10905
2004
Escherichia coli (P22256), Escherichia coli
Manually annotated by BRENDA team
Liu, W.; Peterson, P.E.; Langston, J.A.; Jin, X.; Zhou, X.; Fisher, A.J.; Toney, M.D.
Kinetic and crystallographic analysis of active site mutants of Escherichia coli gamma-aminobutyrate aminotransferase
Biochemistry
44
2982-2992
2005
Escherichia coli (P22256), Escherichia coli
Manually annotated by BRENDA team
Nonaka, S.; Someya, T.; Zhou, S.; Takayama, M.; Nakamura, K.; Ezura, H.
An Agrobacterium tumefaciens strain with gamma-aminobutyric acid transaminase activity shows an enhanced genetic Transformation Ability in Plants
Sci. Rep.
7
42649
2017
Agrobacterium tumefaciens (A0A5B8KQ12), Agrobacterium tumefaciens (Q8UBL5), Agrobacterium tumefaciens, Escherichia coli (P22256), Agrobacterium tumefaciens GV2260 (Q8UBL5), Agrobacterium tumefaciens C58 (Q8UBL5)
Manually annotated by BRENDA team