Information on EC 2.6.1.14 - asparagine-oxo-acid transaminase

New: Word Map on EC 2.6.1.14
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Murinae

EC NUMBER
COMMENTARY
2.6.1.14
-
RECOMMENDED NAME
GeneOntology No.
asparagine-oxo-acid transaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-asparagine + a 2-oxo carboxylate = 2-oxosuccinamate + an amino acid
show the reaction diagram
ping-pong mechanism
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Alanine, aspartate and glutamate metabolism
-
-
aspartate and asparagine metabolism
-
-
L-asparagine degradation II
-
-
SYSTEMATIC NAME
IUBMB Comments
L-asparagine:2-oxo-acid aminotransferase
A pyridoxal-phosphate protein.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
aminotransferase, asparagine-keto acid
-
-
-
-
asparagine keto-acid transaminase
-
-
-
-
asparagine oxoacid aminotransferase
-
-
-
-
asparagine-pyruvate transaminase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-43-7
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxosuccinamic acid + aspartate
L-asparagine + 2-oxo-propane-1,3-dioate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-2-aminobutanoic acid
L-asparagine + 2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-2-aminobutanoic acid
L-asparagine + 2-oxobutanoate
show the reaction diagram
-
-
-
-
-
2-oxosuccinamic acid + L-2-aminobutanoic acid
L-asparagine + 2-oxobutanoate
show the reaction diagram
-
-
-
-
r
2-oxosuccinamic acid + L-alanine
L-asparagine + pyruvate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-alanine
L-asparagine + pyruvate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-cysteine
L-asparagine + 3-mercapto-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-ethionine
L-asparagine + 4-ethylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-glutamine
L-asparagine + ?
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-glutamine
L-asparagine + ?
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-histidine
L-asparagine + 3-(1H-imidazol-4-yl)-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-homocysteine
L-asparagine + 4-mercapto-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-homoserine
L-asparagine + 4-hydroxy-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-leucine
L-asparagine + 4-methyl-2-oxopentanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-leucine
L-asparagine + 4-methyl-2-oxopentanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-methionine
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
r
2-oxosuccinamic acid + L-methionine
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-methionine
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
r
2-oxosuccinamic acid + L-methylcysteine
L-asparagine + ?
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-norleucine
L-asparagine + 2-oxohexanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-norvaline
L-asparagine + 2-oxopentanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-norvaline
L-asparagine + 2-oxopentanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-phenylalanine
L-asparagine + 2-oxo-3-phenylpropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-serine
L-asparagine + 3-hydroxy-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-tryptophan
L-asparagine + 3-indole-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-tyrosine
L-asparagine + 3-(4-hydroxyphenyl)-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
glyoxylate + amino acid
?
show the reaction diagram
-
-
-
-
-
glyoxylate + L-asparagine
? + 2-oxosuccinamate
show the reaction diagram
-
-
-
-
?
L-asparagine + 2-oxobutanoate
2-oxosuccinamate + 2-aminobutanoate
show the reaction diagram
-
weak
-
-
r
L-asparagine + 2-oxoisohexanoate
2-oxosuccinamate + 2-aminoisohexanoate
show the reaction diagram
-
-
-
-
?
L-asparagine + 2-oxosuccinamate
2-oxosuccinamate + L-asparagine
show the reaction diagram
-
-
-
-
?
L-asparagine + 4-hydroxy-2-oxoglutarate
2-oxosuccinamate + 2-amino-4-hydroxyglutarate
show the reaction diagram
-
-
-
-
?
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
2-oxosuccinamate + L-methionine
show the reaction diagram
-
-
-
-
r
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
2-oxosuccinamate + L-methionine
show the reaction diagram
-
weak
-
-
r
L-asparagine + glyoxylate
2-oxosuccinamate + glycine
show the reaction diagram
-
-
-
-
?
L-asparagine + glyoxylate
2-oxosuccinamate + glycine
show the reaction diagram
-
-
-
-
?
L-asparagine + pyruvate
2-oxosuccinamate + L-alanine
show the reaction diagram
-
-
-
-
-
L-asparagine + pyruvate
2-oxosuccinamate + L-alanine
show the reaction diagram
-
-
-
-
-
phenylpyruvate + L-asparagine
L-phenylalanine + 2-oxosuccinamate
show the reaction diagram
-
-
-
-
?
phenylpyruvate + L-asparagine
L-phenylalanine + 2-oxosuccinamate
show the reaction diagram
-
-
-
-
?
phenylpyruvate + L-glutamine
L-phenylalanine + 2-oxoglutaramate
show the reaction diagram
-
-
-
-
?
phenylpyruvate + L-homoserine
phenylalanine + 4-hydroxy-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
phenylpyruvate + methionine
L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
L-phenylalanine + pyruvate
phenylpyruvate + L-alanine
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the following 2-oxo acids are inactive at concentrations of 20 mM: 2-oxoglutarate, oxaloacetate, 2-oxoisopentanoate, L-2-oxo-3-methylvalerate, 3-sulfopyruvate, 3-hydroxy-2-oxobutanoate, trimethylpyruvate
-
-
-
additional information
?
-
-
no or very low activity with: L-valine, L-isoleucine, L-alloisoleucine, L-tertiary-leucine, L-2-aminophenylacetic acid, L-glutamic acid, L-aspartic acid
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glyoxylate + amino acid
?
show the reaction diagram
-
-
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
a pyridoxal phosphate protein
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(NH4)2SO4
-
at high concentration
AgNO3
-
-
Aminooxyacetate
-
-
D-cycloserine
-
weak
glycine
-
asparagine/pyruvate reaction
HgCl2
-
-
homoserine
-
asparagine/pyruvate reaction; DL-isomer
homoserine
-
asparagine/pyruvate reaction
hydroxylamine
-
-
L-2-aminobutanoate
-
weak, asparagine/pyruvate reaction
L-alanine
-
weak, asparagine/pyruvate reaction
L-aspartate
-
-
L-aspartate
-
weak, asparagine/pyruvate reaction
L-cysteine
-
-
L-glutamate
-
weak, asparagine/pyruvate reaction
L-glutamine
-
-
L-glutamine
-
asparagine/pyruvate reaction
L-methionine-SR-sulfoxime
-
asparagine/pyruvate reaction
leucine
-
asparagine/pyruvate reaction; weak
leucine
-
asparagine/pyruvate reaction
methionine
-
asparagine/pyruvate reaction; L-isomer
N-ethylmaleimide
-
-
norvaline
-
asparagine/pyruvate reaction
p-chloromercuribenzoate
-
-
phenylalanine
-
-
phenylalanine
-
-
S-methyl-L-cysteine
-
asparagine/pyruvate reaction
serine
-
asparagine/pyruvate reaction
serine
-
asparagine/pyruvate reaction
methionine
-
asparagine/pyruvate reaction
additional information
-
strong substrate and product inhibition
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
28.8
2-aminoisobutanoate
-
cosubstrate 2-oxosuccinamate
100
2-oxobutanoate
-
cosubstrate L-asparagine
2.5
2-oxosuccinamate
-
cosubstrate L-asparagine
0.35
4-methylsulfanyl-2-oxobutanoate
-
cosubstrate L-asparagine
0.077
glyoxylate
-
cosubstrate L-asparagine
4.8
L-alanine
-
cosubstrate 2-oxosuccinamate
1
L-asparagine
-
cosubstrate phenylpyruvate
0.43
L-glutamine
-
cosubstrate phenylpyruvate
21.4
L-glutamine
-
cosubstrate 2-oxosuccinamate
24.7
L-homocysteine
-
cosubstrate 2-oxosuccinamate
1.4
L-homoserine
-
cosubstrate phenylpyruvate
28.7
L-leucine
-
cosubstrate 2-oxosuccinamate
0.03
L-methionine
-
cosubstrate phenylpyruvate
6.8
L-methionine
-
cosubstrate 2-oxosuccinamate
6.5
L-norvaline
-
cosubstrate 2-oxosuccinamate
0.45
phenylpyruvate
-
cosubstrate L-asparagine
5.1
pyruvate
-
cosubstrate L-asparagine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.08
-
-
additional information
-
similar activity in glycylglycine, ammediol and Tris/HCl buffer
additional information
-
highest activity at pH 8.5 in N,N-bis(2-hydroxyethyl)glycine buffer and 4-(2-hydroxyethyl)-1-piperazinepropane sulfonic acid buffer, low activity in borate buffer and glycylglycine buffer
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.2
-
assay at
8.5 - 8.6
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
-
no activity in rat brain and skeletal muscle
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
70800
-
mouse mitochondrial enzyme
637254
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE