Information on EC 2.6.1.14 - asparagine-oxo-acid transaminase

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The expected taxonomic range for this enzyme is: Murinae

EC NUMBER
COMMENTARY
2.6.1.14
-
RECOMMENDED NAME
GeneOntology No.
asparagine-oxo-acid transaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-asparagine + a 2-oxo carboxylate = 2-oxosuccinamate + an amino acid
show the reaction diagram
ping-pong mechanism
-
L-asparagine + a 2-oxo carboxylate = 2-oxosuccinamate + an amino acid
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
amino group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Alanine, aspartate and glutamate metabolism
-
asparagine degradation II
-
SYSTEMATIC NAME
IUBMB Comments
L-asparagine:2-oxo-acid aminotransferase
A pyridoxal-phosphate protein.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminotransferase, asparagine-keto acid
-
-
-
-
asparagine keto-acid transaminase
-
-
-
-
asparagine oxoacid aminotransferase
-
-
-
-
asparagine-pyruvate transaminase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9030-43-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxosuccinamic acid + aspartate
L-asparagine + 2-oxo-propane-1,3-dioate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-2-aminobutanoic acid
L-asparagine + 2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-2-aminobutanoic acid
L-asparagine + 2-oxobutanoate
show the reaction diagram
-
-
-
-
-
2-oxosuccinamic acid + L-2-aminobutanoic acid
L-asparagine + 2-oxobutanoate
show the reaction diagram
-
-
-
-
r
2-oxosuccinamic acid + L-alanine
L-asparagine + pyruvate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-alanine
L-asparagine + pyruvate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-cysteine
L-asparagine + 3-mercapto-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-ethionine
L-asparagine + 4-ethylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-glutamine
L-asparagine + ?
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-glutamine
L-asparagine + ?
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-histidine
L-asparagine + 3-(1H-imidazol-4-yl)-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-homocysteine
L-asparagine + 4-mercapto-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-homoserine
L-asparagine + 4-hydroxy-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-leucine
L-asparagine + 4-methyl-2-oxopentanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-leucine
L-asparagine + 4-methyl-2-oxopentanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-methionine
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
r
2-oxosuccinamic acid + L-methionine
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-methionine
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
r
2-oxosuccinamic acid + L-methylcysteine
L-asparagine + ?
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-norleucine
L-asparagine + 2-oxohexanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-norvaline
L-asparagine + 2-oxopentanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-norvaline
L-asparagine + 2-oxopentanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-phenylalanine
L-asparagine + 2-oxo-3-phenylpropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-serine
L-asparagine + 3-hydroxy-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-tryptophan
L-asparagine + 3-indole-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
2-oxosuccinamic acid + L-tyrosine
L-asparagine + 3-(4-hydroxyphenyl)-2-oxopropanoate
show the reaction diagram
-
-
-
-
?
glyoxylate + amino acid
?
show the reaction diagram
-
-
-
-
-
glyoxylate + L-asparagine
? + 2-oxosuccinamate
show the reaction diagram
-
-
-
-
?
L-asparagine + 2-oxobutanoate
2-oxosuccinamate + 2-aminobutanoate
show the reaction diagram
-
weak
-
-
r
L-asparagine + 2-oxoisohexanoate
2-oxosuccinamate + 2-aminoisohexanoate
show the reaction diagram
-
-
-
-
?
L-asparagine + 2-oxosuccinamate
2-oxosuccinamate + L-asparagine
show the reaction diagram
-
-
-
-
?
L-asparagine + 4-hydroxy-2-oxoglutarate
2-oxosuccinamate + 2-amino-4-hydroxyglutarate
show the reaction diagram
-
-
-
-
?
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
2-oxosuccinamate + L-methionine
show the reaction diagram
-
-
-
-
r
L-asparagine + 4-methylsulfanyl-2-oxobutanoate
2-oxosuccinamate + L-methionine
show the reaction diagram
-
weak
-
-
r
L-asparagine + glyoxylate
2-oxosuccinamate + glycine
show the reaction diagram
-
-
-
-
?
L-asparagine + glyoxylate
2-oxosuccinamate + glycine
show the reaction diagram
-
-
-
-
?
L-asparagine + pyruvate
2-oxosuccinamate + L-alanine
show the reaction diagram
-
-
-
-
-
L-asparagine + pyruvate
2-oxosuccinamate + L-alanine
show the reaction diagram
-
-
-
-
-
phenylpyruvate + L-asparagine
L-phenylalanine + 2-oxosuccinamate
show the reaction diagram
-
-
-
-
?
phenylpyruvate + L-asparagine
L-phenylalanine + 2-oxosuccinamate
show the reaction diagram
-
-
-
-
?
phenylpyruvate + L-glutamine
L-phenylalanine + 2-oxoglutaramate
show the reaction diagram
-
-
-
-
?
phenylpyruvate + L-homoserine
phenylalanine + 4-hydroxy-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
phenylpyruvate + methionine
L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate
show the reaction diagram
-
-
-
-
?
L-phenylalanine + pyruvate
phenylpyruvate + L-alanine
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the following 2-oxo acids are inactive at concentrations of 20 mM: 2-oxoglutarate, oxaloacetate, 2-oxoisopentanoate, L-2-oxo-3-methylvalerate, 3-sulfopyruvate, 3-hydroxy-2-oxobutanoate, trimethylpyruvate
-
-
-
additional information
?
-
-
no or very low activity with: L-valine, L-isoleucine, L-alloisoleucine, L-tertiary-leucine, L-2-aminophenylacetic acid, L-glutamic acid, L-aspartic acid
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glyoxylate + amino acid
?
show the reaction diagram
-
-
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
a pyridoxal phosphate protein
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(NH4)2SO4
-
at high concentration
Aminooxyacetate
-
-
D-cycloserine
-
weak
glycine
-
asparagine/pyruvate reaction
homoserine
-
asparagine/pyruvate reaction; DL-isomer
homoserine
-
asparagine/pyruvate reaction
hydroxylamine
-
-
L-2-aminobutanoate
-
weak, asparagine/pyruvate reaction
L-alanine
-
weak, asparagine/pyruvate reaction
L-aspartate
-
weak, asparagine/pyruvate reaction
L-cysteine
-
-
L-glutamate
-
weak, asparagine/pyruvate reaction
L-glutamine
-
asparagine/pyruvate reaction
L-methionine-SR-sulfoxime
-
asparagine/pyruvate reaction
leucine
-
asparagine/pyruvate reaction; weak
leucine
-
asparagine/pyruvate reaction
methionine
-
asparagine/pyruvate reaction; L-isomer
N-ethylmaleimide
-
-
norvaline
-
asparagine/pyruvate reaction
p-chloromercuribenzoate
-
-
phenylalanine
-
-
phenylalanine
-
-
S-methyl-L-cysteine
-
asparagine/pyruvate reaction
serine
-
asparagine/pyruvate reaction
serine
-
asparagine/pyruvate reaction
methionine
-
asparagine/pyruvate reaction
additional information
-
strong substrate and product inhibition
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
28.8
-
2-aminoisobutanoate
-
cosubstrate 2-oxosuccinamate
100
-
2-oxobutanoate
-
cosubstrate L-asparagine
2.5
-
2-oxosuccinamate
-
cosubstrate L-asparagine
0.35
-
4-methylsulfanyl-2-oxobutanoate
-
cosubstrate L-asparagine
0.077
-
glyoxylate
-
cosubstrate L-asparagine
4.8
-
L-alanine
-
cosubstrate 2-oxosuccinamate
1
-
L-asparagine
-
cosubstrate phenylpyruvate
0.43
-
L-glutamine
-
cosubstrate phenylpyruvate
21.4
-
L-glutamine
-
cosubstrate 2-oxosuccinamate
24.7
-
L-homocysteine
-
cosubstrate 2-oxosuccinamate
1.4
-
L-homoserine
-
cosubstrate phenylpyruvate
28.7
-
L-leucine
-
cosubstrate 2-oxosuccinamate
0.03
-
L-methionine
-
cosubstrate phenylpyruvate
6.8
-
L-methionine
-
cosubstrate 2-oxosuccinamate
6.5
-
L-norvaline
-
cosubstrate 2-oxosuccinamate
0.45
-
phenylpyruvate
-
cosubstrate L-asparagine
5.1
-
pyruvate
-
cosubstrate L-asparagine
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.08
-
-
-
additional information
-
-
similar activity in glycylglycine, ammediol and Tris/HCl buffer
additional information
-
-
highest activity at pH 8.5 in N,N-bis(2-hydroxyethyl)glycine buffer and 4-(2-hydroxyethyl)-1-piperazinepropane sulfonic acid buffer, low activity in borate buffer and glycylglycine buffer
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.2
-
-
assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
additional information
-
no activity in rat brain and skeletal muscle
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
70800
-
-
mouse mitochondrial enzyme
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE