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Information on EC 2.5.1.6 - methionine adenosyltransferase and Organism(s) Saccharolobus solfataricus and UniProt Accession Q980S9
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2.5.1.6 methionine adenosyltransferaseSpecify your search results
Word Map
- 2.5.1.6
- monolayers
-
self-assembled
- gold
-
film
- alkanethiols
-
photoelectron
-
infrared
-
fabric
-
electrode
-
electrochemical
-
tunnel
-
voltammetry
-
coverage
-
interfacial
-
ellipsometry
-
impedance
- s-adenosylhomocysteine
-
thiolate
-
silicon
-
photoemission
-
electrochemistry
-
well-ordered
-
photovoltaic
-
wafer
-
large-area
-
transistor
-
transmethylation
-
semiconductor
-
nanoscopic
-
field-effect
-
close-packed
-
stamp
-
photolithography
-
wettabl
-
nanopatterns
-
polycrystalline
-
electroless
-
thin-film
-
chemisorption
- drug development
-
lithography
-
microcontact
-
fermi
-
headgroups
-
wettability
- synthesis
- medicine
-
micropatterned
-
microbalance
-
silane
-
statin-associated
-
transsulfuration
- ferrocene
The taxonomic range for the selected organisms is: Saccharolobus solfataricus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
sams, mat2a, methionine adenosyltransferase, mat1a, s-adenosylmethionine synthetase, adomet synthetase, sam synthetase, mat ii, matalpha2, s-adenosyl-l-methionine synthetase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
adenosylmethionine synthetase
-
-
-
-
AdoMet synthetase
-
-
-
-
ATP-methionine adenosyltransferase
-
-
-
-
methionine adenosyltransferase
-
-
-
-
methionine S-adenosyltransferase
-
-
-
-
methionine-activating enzyme
-
-
-
-
S-adenosyl-L-methionine synthetase
-
-
-
-
S-adenosylmethionine synthase
-
-
-
-
S-adenosylmethionine synthetase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
adenosyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:L-methionine S-adenosyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY
9012-52-6
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + (2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid
10% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid
30% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(butylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(butylselanyl)butanoic acid
70% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(butylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(butylsulfanyl)butanoic acid
27% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(ethylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(ethylselanyl)butanoic acid
76% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(ethylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(ethylsulfanyl)butanoic acid
84% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid
30% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid
27% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid
28% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid
36% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid
40% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid
53% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propylselanyl)butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propylselanyl)butanoic acid
66% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-(propylsulfanyl)butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propylsulfanyl)butanoic acid
44% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid
50% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-methyl propyl)sulfanyl] butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methyl propyl)sulfanyl]butanoic acid
57% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid
46% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid
15% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid
27% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid
25% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid
60% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-methyl butyl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methyl butyl)sulfanyl] butanoic acid
36% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid
44% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid
12% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(cyanomethyl) sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(cyanomethyl)sulfanyl]butanoic acid
68% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid
12% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid
38% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid + H2O
phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid
24% of the turnover rate compared to L-methionine
-
-
?
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid + H2O
phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid
28% of the turnover rate compared to L-methionine
-
-
?
ATP + L-ethionine + H2O
phosphate + diphosphate + S-adenosyl-L-ethionine
-
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
?
ATP + L-selenomethionine + H2O
phosphate + diphosphate + Se-adenosyl-L-selenomethionine
as active as L-methionine
-
-
?
ATP + L-methionine + H2O
phosphate + diphosphate + S-adenosyl-L-methionine
-
-
-
-
?
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
tripolyphosphate + H2O
diphosphate + phosphate
-
tripolyphosphatase activity
-
?
additional information
?
-
the enzyme has the ability to produce a range of differentially alkylated AdoMet analogs in the presence of non-native methionine analogs and ATP
-
-
?
additional information
?
-
-
S-adenosylmethionine synthetase A exhibits tripolyphosphatase activity
-
-
?
additional information
?
-
-
S-adenosylmethionine synthetase B exhibits tripolyphosphatase activity
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + L-methionine + H2O
S-adenosyl-L-methionine + phosphate + diphosphate
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
Mg2+
Mn2+
Co2+
-
can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase B
Mg2+
-
S-adenosylmethionine synthetase A is absolutely dependent upon the presence of Mg2+, suggesting that a Mg-ATP complex functions as substrate. Maximal activity is obtained at an Mg2+ concentration of 40 mM with 10 mM ATP. 30% of the maximal reaction rate is observable at equimolar concentrations of ATP and Mg2+ (10 mM)
Mg2+
-
S-adenosylmethionine synthetase B is absolutely dependent upon the presence of Mg2+, suggesting that a Mg-ATP complex functions as substrate. Maximal activity is obtained at an Mg2+ concentration of 40 mM with 10 mM ATP. 30% of the maximal reaction rate is observable at equimolar concentrations of ATP and Mg2+ (10 mM)
Mn2+
-
can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase A
Mn2+
-
can replace Mg2+ with a lower relative activity, S-adenosylmethionine synthetase B
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
S-adenosyl(5')-3-methylthiopropylamine does not inhibit
-
cycloleucine
-
inhibits only at sub saturating concentrations of methionine
cycloleucine
-
25 mM, 56% inhibition, S-adenosylmethionine synthetase A
diphosphate
-
20 mM, 30% inhibition, S-adenosylmethionine synthetase A
diphosphate
-
20 mM, 49% inhibition, S-adenosylmethionine synthetase B
phosphate
-
10 mM, 19% inhibition, S-adenosylmethionine synthetase B
phosphate
-
10 mM, 45% inhibition, S-adenosylmethionine synthetase A
S-adenosylmethionine
-
non-competitive, S-adenosylmethionine synthetase A
S-adenosylmethionine
-
slight inhibition of S-adenosylmethionine synthetase B
Tetrapolyphosphate
-
10 mM, 40% inhibition, S-adenosylmethionine synthetase B
Tetrapolyphosphate
-
10 mM, 50% inhibition, S-adenosylmethionine synthetase A
tripolyphosphate
-
1.0 mM, 57% inhibition, S-adenosylmethionine synthetase A
tripolyphosphate
-
1.0 mM, 62% inhibition, S-adenosylmethionine synthetase B
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
S-adenosylmethionine
-
activates the tripolyphosphatase activity of A isoform
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.1
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.2 - 9.3
7.2 - 9.3
-
50% of maximal activity at pH 7.2 and at pH 9.3, S-adenosylmethionine synthetase A
7.2 - 9.3
-
50% of maximal activity at pH 7.2 and at pH 9.3, S-adenosylmethionine synthetase B
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
90
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
75 - 110
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
180000
75000
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop method at 20°C, crystallization of the enzyme in the S-adenosylmethionine bound, S-adenosylethionine bound and unbound forms
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
100
additional information
-
preincubation with ATP protects against thermal inactivation
100
-
more than 80% activity is retained in the presence of 10 mM ATP at 100°C for 30 min. GTP, ADP, AMP and methionine do not exert any protective effect, S-adenosylmethionine synthetase A
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, partially purified isoenzymes, 10 mM Tris/HCl, pH 8, stable for 4 weeks
-
-20°C, pH 8, 10 mM Tris/HCl buffer, partially purified S-adenosylmethionine synthetase A is stable for four weeks
-
-20°C, pH 8, 10 mM Tris/HCl buffer, partially purified S-adenosylmethionine synthetase B is stable for four weeks
-
4°C, isoenzyme A and B are spontaneously inactivated in 1 month and 1 week, respectively, in presence of glycerol stability increases up to 90%
-
4°C, S-adenosylmethionine synthetase is spontaneously inactivated in 1 month, S-adenosylmethionine synthetase A
-
4°C, S-adenosylmethionine synthetase is spontaneously inactivated in 1 week
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Porcelli, M.; Cacciapuoti, G.; Carteni-Farina, M.; Gambacorta, A.
S-adenosylmethionine synthetase in the thermophilic archaebacterium Sulfolobus solfataricus. Purification and characterization of two isoforms
Eur. J. Biochem.
177
273-280
1988
Saccharolobus solfataricus
Wang, F.; Singh, S.; Zhang, J.; Huber, T.D.; Helmich, K.E.; Sunkara, M.; Hurley, K.A.; Goff, R.D.; Bingman, C.A.; Morris, A.J.; Thorson, J.S.; Phillips, G.N.
Understanding molecular recognition of promiscuity of thermophilic methionine adenosyltransferase sMAT from Sulfolobus solfataricus
FEBS J.
281
224-239
2014
Saccharolobus solfataricus (Q980S9), Saccharolobus solfataricus P2 (Q980S9)
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