We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa The enzyme appears in selected viruses and cellular organisms
Synonyms
kdo8ps, kdo8p synthase, kdo 8-p synthase, kdo-8-p synthase, kdops, 3-deoxy-d-manno-octulosonate 8-phosphate synthase, kdo-8-phosphate synthetase, 3-deoxy-d-manno-octulosonate-8-phosphate synthase, atkdsa1, 3-deoxy-d-manno-2-octulosonate-8-phosphate synthase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-dehydro-3-deoxy-D-octonate-8-phosphate D-arabinose-5-phosphate-lyase (pyruvate-phosphorylating)
-
-
-
-
2-dehydro-3-deoxyphosphooctonate aldolase
2-keto-3-deoxy-8-phosphooctonic acid synthetase
-
-
-
-
2-keto-3-deoxy-8-phosphooctonic synthetase
-
-
-
-
2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase
-
-
3-deoxy-D-manno-octulosonate 8-phosphate synthetase
-
-
-
-
3-deoxy-D-manno-octulosonate-8-phosphate synthase
-
-
-
-
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
-
-
-
-
3-deoxy-D-mannooctulosonate-8-phosphate synthetase
-
-
-
-
3-deoxyoctulosonic 8-phosphate synthetase
-
-
-
-
8-phospho-2-dehydro-3-deoxy-D-octonate D-arabinose-5-phosphate-lyase (pyruvate-phosphorylating)
-
-
-
-
aldolase, phospho-2-keto-3-deoxyoctonate
-
-
-
-
KDO-8-P synthetase
-
-
-
-
Phospho-2-dehydro-3-deoxyoctonate aldolase
-
-
-
-
phospho-2-keto-3-deoxyoctonate aldolase
-
-
-
-
2-dehydro-3-deoxyphosphooctonate aldolase
-
-
-
-
2-dehydro-3-deoxyphosphooctonate aldolase
-
-
KDO8PS
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
phosphoenolpyruvate:D-arabinose-5-phosphate C-(1-carboxyvinyl)transferase (phosphate-hydrolysing, 2-carboxy-2-oxoethyl-forming)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
phosphoenolpyruvate + D-arabinose 5-phosphate
2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
-
?
phosphoenolpyruvate + D-arabinose 5-phosphate
2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
-
-
-
?
phosphoenolpyruvate + D-arabinose 5-phosphate
2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
-
-
?
phosphoenolpyruvate + D-arabinose 5-phosphate
2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
absolute specificity for phosphoenolpyruvate and D-arabinose-5-phosphate
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Co2+
-
1 mM, 22% inhibition
Cu2+
-
1 mM, 80% inhibition
Fe2+
-
1 mM, 30% inhibition
Mn2+
-
1 mM, 40% inhibition
Zn2+
-
1 mM, 34% inhibition
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.1 - 9
-
pH 6.1: about 35% of maximal activity, pH 9.0: about 25% of maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
the enzyme catalyzes a key step in the synthesis of the lipopolysaccharide of most Gram-negative bacteria, it catalyzes the condensation reaction between phosphoenolpyruvate and arabinose 5-phosphate to produce 3-deoxy-D-manno-octulosonate 8-phosphate. The enzyme is vital for for proper synthesis and assembly of lipoplysaccharides and the survival and virulence of Pseudomonas aeruginosa
additional information
-
active site structure, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
oligomer
-
the enzyme structure is a prototypical alpha/beta TIM barrel structure expected from this family of enzymes and contains a tetramer in the asymmetric unit
additional information
-
two-dimensional structure comparison
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
purified recombinant His6-tagged enzyme, sitting drop vapour diffusion method, mixing of 0.003 ml of 5 mg/ml protein in 0.2 M Tris-HCl, pH 7.4, 0.1 M NaCl, 0.05% 2-mercaptoethanol, and 0.1 mM phosphoenolpyruvate with 0.0015 ml of reservoir solution containing 0.2 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5, 18% w/v PEG 8000, 20°C, 2 days, X-ray diffraction structure determination and analysis at 2.8 A resolution, molecular replacementusing structure PDB ID 1g7v and model building
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dialysis against 0.01 M Tris-HCl, pH 7.5, containing 0.01 M thioglycerol for 4 h, less than 20% loss of activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
recombinant His6-tagged enzyme from Escherichia coli strain Rosetta2 (pLysS) DE3 by nickel affinity chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gene kdo8ps, recombinant expression of His6-tagged codon-optimized enzyme in Escherichia coli strain Rosetta2 (pLysS) DE3
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Levin, D.H.; Racker, E.
Condensation of arabinose 5-phosphate and phosphorylenol pyruvate by 2-keto-3-deoxy-8-phosphooctonic acid synthetase
J. Biol. Chem.
234
2532-2539
1959
Pseudomonas aeruginosa
brenda
Levin, D.H.; Racker, E.
2-Keto-3-deoxy-8-phosphooctonic acid synthetase
Methods Enzymol.
8
216-221
1966
Pseudomonas aeruginosa
-
brenda
Birck, M.R.; Woodard, R.W.
Aquifex aeolicus 3-Deoxy-D-manno-2-octulosonic acid 8-phosphate synthase: A new class of KDO 8-P synthase?
J. Mol. Evol.
52
205-214
2001
Actinobacillus pleuropneumoniae (O68662), Aggregatibacter actinomycetemcomitans, Aquifex aeolicus, Aquifex aeolicus (O66496), Arabidopsis thaliana, Bordetella bronchiseptica, Bordetella pertussis, Campylobacter jejuni, Caulobacter vibrioides, Chlamydia pneumoniae (Q9Z7I4), Chlamydia psittaci (Q46225), Chlamydia trachomatis (P0CD74), Chlorobaculum tepidum, Escherichia coli (P0A715), Escherichia coli, Haemophilus influenzae (P45251), Helicobacter pylori, Helicobacter pylori (Q9ZN55), Klebsiella pneumoniae, Mannheimia haemolytica (P95514), Neisseria gonorrhoeae (Q9XB02), Neisseria meningitidis (Q9XAZ3), Pasteurella multocida, Pisum sativum, Porphyromonas gingivalis, Pseudomonas aeruginosa (Q9ZFK4), Rickettsia prowazekii (Q9ZE84), Salmonella enterica subsp. enterica serovar Typhi, Shewanella putrefaciens, Vibrio cholerae serotype O1, Yersinia pestis
brenda
Nelson, S.K.; Kelleher, A.; Robinson, G.; Reiling, S.; Asojo, O.A.
Structure of 2-keto-3-deoxy-D-manno-octulosonate-8-phosphate synthase from Pseudomonas aeruginosa
Acta Crystallogr. Sect. F
69
1084-1088
2013
Pseudomonas aeruginosa
brenda