Information on EC 2.5.1.52 - L-mimosine synthase

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The expected taxonomic range for this enzyme is: Leucaena leucocephala

EC NUMBER
COMMENTARY
2.5.1.52
-
RECOMMENDED NAME
GeneOntology No.
L-mimosine synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
O3-acetyl-L-serine + 3,4-dihydroxypyridine = 3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
aminopropanoyl-group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
mimosine biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
O3-acetyl-L-serine:3,4-dihydroxypyridine 1-(2-amino-2-carboxyethyl)transferase
Brings about the biosynthesis of L-mimosine in Mimosa and Leucaena sp. Not identical with EC 2.5.1.51, beta-pyrazolylalanine synthase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
EC 4.2.99.15
-
-
formerly
-
O3-acetyl-L-serine acetate-lyase (adding 3,4-dihydroxypyridin-1-yl)
-
-
-
-
synthase, L-mimosine
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
93229-75-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O-succinyl-L-serine + 3,4-dihydroxypyridine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + succinate
show the reaction diagram
-
60% of the activity with O-acetylserine
-
-
?
O-sulfo-L-serine + 3,4-dihydroxypyridine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + sulfate
show the reaction diagram
-
32% of the activity with O-acetyl-L-serine
-
-
?
O3-acetyl-L-serine + 3,4-dihydroxypyridine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
show the reaction diagram
-
-
i.e. L-mimosine
?
O3-acetyl-L-serine + 3,4-dihydroxypyridine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
show the reaction diagram
-
best substrates
i.e. L-mimosine
?
O3-acetyl-L-serine + 3,4-dihydroxypyridine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
show the reaction diagram
-
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
-
-
-
O3-acetyl-L-serine + 3-amino-1,2,4-triazole
3-(3-amino-1,2,4-triazol-1-yl)-L-alanine + acetate
show the reaction diagram
-
30% of the activity with 3,4-dihydroxypyridine
-
?
O3-acetyl-L-serine + N-hydroxyurea
ureidoserine + acetate
show the reaction diagram
-
13% of the activity with 3,4-dihydroxypyridine
-
?
O3-acetyl-L-serine + pyrazole
3-(pyrazol-1-yl)-L-alanine + acetate
show the reaction diagram
-
82% of the activity with 3,4-dihydroxypyridine
-
?
additional information
?
-
-
no activity with O-phospho-L-serine and L-Ser
-
-
-
additional information
?
-
-
no activity with alpha,beta-diaminopropionic acid
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
O3-acetyl-L-serine + 3,4-dihydroxypyridine
3-(3,4-dihydroxypyridin-1-yl)-L-alanine + acetate
show the reaction diagram
-
involved in biosynthesis of heterocyclic beta-substituted alanines, brings about the biosynthesis of L-mimosine
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
requirement, enzyme bound, 1 mol pyridoxal 5'-phosphate per mol subunit
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
no substrate inhibition
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5
-
3,4-dihydroxypyridine
-
-
6.25
-
O-acetylserine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.8
-
-
reaction with 3,4-dihydroxypyridine
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
cotyledons removed
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
64000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 32000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial, to near homogeneity
-