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Information on EC 2.5.1.18 - glutathione transferase and Organism(s) Oryza sativa and UniProt Accession Q93WY5

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IUBMB Comments
A group of enzymes of broad specificity. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. Also catalyses the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrile, certain isomerization reactions and disulfide interchange.
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This record set is specific for:
Oryza sativa
UNIPROT: Q93WY5
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Word Map
The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
gst, glutathione s-transferase, gstm1, gstp1, gstt1, glutathione-s-transferase, glutathione transferase, gsta1, gst pi, gstm3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GSTU17
tau class glutathione S-transferase
-
tau class glutathione transferase
-
tau class GST
-
glutathione S-alkyl transferase
-
-
-
-
glutathione S-aralkyltransferase
-
-
-
-
glutathione S-aryltransferase
-
-
-
-
glutathione S-transferase
glutathione S-transferase X
-
-
-
-
GSH S-transferase
-
-
-
-
GSHTase-P
-
-
-
-
GSTF3
-
phi class isozyme
GSTF5
-
phi class isozyme
GSTU3
-
tau class isozyme
GSTU4
-
tau class isozyme
GSTU5
-
tau class isozyme
S-(hydroxyalkyl)glutathione lyase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aryl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
RX:glutathione R-transferase
A group of enzymes of broad specificity. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. Also catalyses the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrile, certain isomerization reactions and disulfide interchange.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-37-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glutathione + 1-chloro-2,4-dinitrobenzene
chloride + 2,4-dinitrophenyl-glutathione
show the reaction diagram
-
-
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
S-(2,4-dinitrophenyl)glutathione + HCl
show the reaction diagram
-
-
-
?
glutathione + 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole
?
show the reaction diagram
-
-
-
?
glutathione + 4-nitrobenzo-2-oxa-1,3-diazole
?
show the reaction diagram
-
-
-
?
glutathione + 4-nitrobenzyl chloride
chloride + 4-nitrobenzyl-glutathione
show the reaction diagram
-
-
-
?
glutathione + 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole
?
show the reaction diagram
-
-
-
?
glutathione + cumene hydroperoxide
?
show the reaction diagram
glutathione + 1-chloro-2,4-dinitrobenzene
chloride + 2,4-dinitrophenyl-glutathione
show the reaction diagram
-
-
-
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
S-(2,4-dinitrophenyl)glutathione + HCl
show the reaction diagram
-
-
-
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
S-2,4-dinitrophenylglutathione + HCl
show the reaction diagram
-
-
-
-
?
glutathione + acetochlor
?
show the reaction diagram
-
-
-
-
?
glutathione + alachlor
?
show the reaction diagram
-
-
-
-
?
glutathione + cumene hydroperoxide
?
show the reaction diagram
-
-
-
-
?
glutathione + ethacrynic acid
?
show the reaction diagram
-
-
-
-
?
glutathione + fenchlorim
?
show the reaction diagram
-
-
-
-
?
glutathione + fluorodifen
4-nitrophenol + (2-nitro-4-trifluoromethylphenyl)glutathione
show the reaction diagram
-
-
-
-
?
glutathione + metolachlor
?
show the reaction diagram
-
-
-
-
?
glutathione + petrilachlor
?
show the reaction diagram
-
-
-
-
?
glutathione + pretilachlor
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bromosulfophthalein
-
-
chlorotriphenyltin
-
-
Cibacron blue
-
-
Cu2+
-
about 52% inhibition at 0.008 mM
iodoacetate
-
-
S-hexylglutathione
-
-
tridiphane
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.324 - 3.866
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
0.324
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
pH 7.4, 37°C
0.058 - 0.743
glutathione
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.257 - 1.75
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
0.257
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
pH 7.4, 37°C
0.196 - 0.946
glutathione
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.221 - 1.177
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
0.013
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole
pH 7.4, 37°C
0.076 - 4.552
glutathione
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.203
GSTU17, substrate 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole
82.27
-
GST I-I
93.21
-
GST II-III
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
GST I-I
7
-
GST II-III
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8.5
activity range of GSTU17
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low expression of GSTU17
Manually annotated by BRENDA team
high expression of GSTU17 in mature, lower in young leaves, no expression in immature leaves
Manually annotated by BRENDA team
low expression of GSTU17
Manually annotated by BRENDA team
low expression of GSTU17
Manually annotated by BRENDA team
-
young and mature
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
-
expression patterns of rice GST genes in various tissues/organs and developmental stages, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the classical phase II detoxification glutathione transferases are key metabolic enzymes that catalyze the conjugation of glutathione to various electrophilic compounds
physiological function
-
overlapping and specific roles of rice glutathione S-transferase genes during development and stress responses, role of GSTs in mediating crosstalk between various stress and hormone response pathways
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25180
x * 25180, GSTU17, sequence calculation
25000
-
1 * 25000 + 1 * 28000, GST II-III, SDS-PAGE
28000
-
1 * 25000 + 1 * 28000, GST II-III, SDS-PAGE
30000
-
2 * 30000, GST I, SDS-PAGE
45000 - 60000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E68A
the mutant retains activity toward 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole, 1-chloro-2,4-dinitrobenzene and 4-nitrobenzyl chloride, but its activities drop significantly compared to the wild type enzyme
F116A
the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme
F162A
the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme
K42A
the mutant shows 10% of wild type activity
L112A
the mutant shows reduced catalytic efficiency toward glutathione and increased catalytic efficiency toward 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme
M17A
the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme
N109A
the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme
S15A
inactive
S69A
the mutant loses activity to 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole while it retains 31% of the activity toward 1-chloro-2,4-dinitrobenzene and 7-8% of the activity toward 4-nitrobenzyl chloride, respectively, compared with the wild type enzyme
V56A
the mutant loses activity to 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole while it retains 21% of the activity toward 1-chloro-2,4-dinitrobenzene and 7-8% of the activity toward 4-nitrobenzyl chloride, respectively, compared with the wild type enzyme
W161A
the mutant shows reduced catalytic efficiency toward glutathione and increased catalytic efficiency toward 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme
Y113A
the mutant shows reduced catalytic efficiency toward glutathione and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole as compared to the wild type enzyme
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 55
the enzyme retains about 85, 99, 95 and 65% activity after 15 min at 25, 35, 45 and 55°C, respectively. The enzyme is inactivated at 65°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-Sepharose column chromatography
glutathione Sepharose column chromatography
-
homogeneity, 2 isoenzymes: GST I-I and GST II-III
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
tau class GST gene OsGSTU17, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged GSTU17 in Escherichia coli strain Bl21
79 genes encoding GSTs, DNA and amino acid sequence determination and analysis, sequence compariosns and phylogenetic analysis, detailed overview
-
expressed in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
at least 31 GST genes show response to plant hormones auxin and cytokinin, differential expression of quite a large number of GST genes during various abiotic stress, arsenate stress and biotic stress conditions, detailed overview
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Deng, F.; Hatzios, K.K.
Purification and characterization of two glutathione S-transferase isozymes from Indica-type rice involved in herbicide detoxification
Pestic. Biochem. Physiol.
72
10-23
2002
Oryza sativa
-
Manually annotated by BRENDA team
Cho, H.Y.; Kong, K.H.
Study on the biochemical characterization of herbicide detoxification enzyme, glutathione S-transferase
Biofactors
30
281-287
2007
Homo sapiens, Oryza sativa
Manually annotated by BRENDA team
Jain, M.; Ghanashyam, C.; Bhattacharjee, A.
Comprehensive expression analysis suggests overlapping and specific roles of rice glutathione S-transferase genes during development and stress responses
BMC Genomics
11
73
2010
Oryza sativa
Manually annotated by BRENDA team
Yang, X.; Sun, W.; Liu, J.P.; Liu, Y.J.; Zeng, Q.Y.
Biochemical and physiological characterization of a tau class glutathione transferase from rice (Oryza sativa)
Plant Physiol. Biochem.
47
1061-1068
2009
Oryza sativa (Q93WY5), Oryza sativa
Manually annotated by BRENDA team
Yang, X.; Wei, J.; Wu, Z.; Gao, J.
Effects of substrate-binding site residues on the biochemical properties of a tau class glutathione S-transferase from Oryza sativa
Genes (Basel)
11
25
2019
Oryza sativa (Q93WY5), Oryza sativa
Manually annotated by BRENDA team
Dobritzsch, D.; Grancharov, K.; Hermsen, C.; Krauss, G.J.; Schaumloeffel, D.
Inhibitory effect of metals on animal and plant glutathione transferases
J. Trace Elem. Med. Biol.
57
48-56
2020
Armoracia rusticana, Astacus astacus, Brassica juncea, Oryctolagus cuniculus, Lemna minor, Meleagris gallopavo, Mus musculus, Oncorhynchus mykiss, Oreochromis mossambicus, Oryza sativa, Pacifastacus leniusculus, Picea abies, Pleuronectes platessa, Populus sp., Pelophylax ridibundus, Rattus norvegicus, Typha latifolia, Rhabdosargus sarba, Chelon saliens, Protaetia brevitarsis, Alburnus tarichi, Salvelinus alpinus, Siganus canaliculatus, Laeonereis acuta
Manually annotated by BRENDA team