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Information on EC 2.5.1.18 - glutathione transferase and Organism(s) Onchocerca volvulus and UniProt Accession P46427

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IUBMB Comments
A group of enzymes of broad specificity. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. Also catalyses the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrile, certain isomerization reactions and disulfide interchange.
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This record set is specific for:
Onchocerca volvulus
UNIPROT: P46427
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Word Map
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The taxonomic range for the selected organisms is: Onchocerca volvulus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
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RX
+
glutathione
=
HX
+
R-S-glutathione
+
=
+
Synonyms
gst, glutathione s-transferase, gstm1, gstp1, gstt1, glutathione-s-transferase, glutathione transferase, gsta1, gst pi, gstm3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glutathione S-transferase
-
glutathione S-alkyl transferase
-
-
-
-
glutathione S-aralkyltransferase
-
-
-
-
glutathione S-aryltransferase
-
-
-
-
glutathione S-transferase
-
-
-
-
glutathione S-transferase X
-
-
-
-
GSH S-transferase
-
-
-
-
GSHTase-P
-
-
-
-
S-(hydroxyalkyl)glutathione lyase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aryl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
RX:glutathione R-transferase
A group of enzymes of broad specificity. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. Also catalyses the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrile, certain isomerization reactions and disulfide interchange.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-37-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glutathione + 1-chloro-2,4-dinitrobenzene
S-(2,4-dinitrophenyl)glutathione + HCl
show the reaction diagram
-
-
-
?
glutathione + 4-hydroxynon-2-enal
?
show the reaction diagram
-
-
-
?
glutathione + cumene hydroperoxide
?
show the reaction diagram
-
-
-
?
glutathione + hexa-2,4-dienal
?
show the reaction diagram
-
-
-
?
glutathione + trans-2-nonenal
?
show the reaction diagram
-
-
-
?
glutathione + trans-trans-2,4-decadienal
?
show the reaction diagram
-
-
-
?
additional information
?
-
no detectable activity with 1,2-dichloro-4-nitrobenzene, ethacrynic acid, bromosulphthalein, and 1,2-epoxy-3-(4-nitrophenoxy) propane
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-Amino-4-{[4-({4-chloro-6-[(2-sulfophenyl)amino]-1,3,5-triazin-2-yl}amino)-3-sulfophenyl]amino}-9,10-dioxo-9,10-dihydro-2-anthracenesulfonic acid
-
Bromosulphthalein
-
Triphenyltin chloride
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.71
1-chloro-2,4-dinitrobenzene
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00075
Bromosulphthalein
Onchocerca volvulus
-
0.00003
Cibacron blue 3GA
Onchocerca volvulus
-
0.00055
haematin
Onchocerca volvulus
-
0.00005
Triphenyltin chloride
Onchocerca volvulus
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GSTP_ONCVO
208
0
24232
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 36000
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant GST2, hanging-drop vapour-diffusion method. Two different crystal forms are grown under identical conditions. They belong to space groups P2(1)2(1)2 and P2(1), respectively. The unit-cell parameters obtained are a = 112.6, b = 84.3, c = 45.1 A from the P2(1)2(1)2 crystal and a = 51.6, b = 82.3, c = 56.7 A, beta = 95.89 degrees from the P2(1) form
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant GST2
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of GST2 in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hoppner, J.; Perbandt, M.; Betzel, C.; Walter, R.D.; Liebau, E.
Crystallization of the major cytosolic glutathione S-transferase from Onchocerca volvulus
Acta Crystallogr. Sect. D
60
1496-1497
2004
Onchocerca volvulus
Manually annotated by BRENDA team
Torres-Rivera, A.; Landa, A.
Glutathione transferases from parasites: a biochemical view
Acta Trop.
105
99-112
2008
Homo sapiens, Echinococcus granulosus (O16058), Rattus norvegicus (P04905), Schistosoma mansoni (P15964), Mus musculus (P24472), Schistosoma japonicum (P26624), Onchocerca volvulus (P46427), Ascaris suum (P46436), Fasciola hepatica (P56598), Sus scrofa (P80031), Clonorchis sinensis (Q1L2C7), Taenia solium (Q3ZJN3), Ancylostoma caninum (Q6J1M5), Plasmodium yoelii (Q7REH6), Wuchereria bancrofti (Q86LL8), Plasmodium falciparum (Q8MU52)
Manually annotated by BRENDA team