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Information on EC 2.5.1.18 - glutathione transferase and Organism(s) Triticum aestivum and UniProt Accession P30111

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IUBMB Comments
A group of enzymes of broad specificity. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. Also catalyses the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrile, certain isomerization reactions and disulfide interchange.
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This record set is specific for:
Triticum aestivum
UNIPROT: P30111
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Word Map
The taxonomic range for the selected organisms is: Triticum aestivum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
gst, glutathione s-transferase, gstm1, gstp1, gstt1, glutathione-s-transferase, glutathione transferase, gsta1, gst pi, gstm3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glutathione S-transferase
-
glutathione S-alkyl transferase
-
-
-
-
glutathione S-aralkyltransferase
-
-
-
-
glutathione S-aryltransferase
-
-
-
-
glutathione S-transferase
glutathione S-transferase X
-
-
-
-
GSH S-transferase
-
-
-
-
GSHTase-P
-
-
-
-
S-(hydroxyalkyl)glutathione lyase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aryl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
RX:glutathione R-transferase
A group of enzymes of broad specificity. R may be an aliphatic, aromatic or heterocyclic group; X may be a sulfate, nitrile or halide group. Also catalyses the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrile, certain isomerization reactions and disulfide interchange.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-37-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glutathione + 1-chloro-2,4-dinitrobenzene
S-(2,4-dinitrophenyl)glutathione + HCl
show the reaction diagram
-
-
-
?
1-chloro-2,4-dinitrobenzene + glutathione
S-(2,4-dinitrophenyl)glutathione + HCl
show the reaction diagram
-
-
-
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
chloride + 2,4-dinitrophenyl-glutathione
show the reaction diagram
-
-
-
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
S-(2,4-dinitrophenyl)glutathione + HCl
show the reaction diagram
-
-
-
?
glutathione + 1-chloro-2,4-dinitrobenzene
S-2,4-dinitrophenylglutathione + HCl
show the reaction diagram
-
-
-
-
?
glutathione + butachlor
?
show the reaction diagram
-
-
-
-
?
glutathione + fluorodifen
4-nitrophenol + 2-nitro-4-trifluoromethylphenol-glutathione
show the reaction diagram
-
-
-
-
?
glutathione + terbuthylazine
?
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cantharidic acid
-
-
S-hexylglutathione
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
benoxacor
-
herbicide safener, activates the enzyme in shoots
cantharidin
-
cantharidin significantly stimulates isoform GSTf3 activity
cloquintocet-mexyl
-
herbicide safener, activates the enzyme in shoots
fenchlorazole-ethyl
-
herbicide safener, activates the enzyme in shoots
fenclorim
-
herbicide safener, activates the enzyme in shoots
fluxofenim
-
herbicide safener, activates the enzyme in shoots
oxabetrinil
-
herbicide safener, activates the enzyme in shoots
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2 - 0.753
1-chloro-2,4-dinitrobenzene
0.022
fluorodifen
-
-
0.228 - 0.36
glutathione
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.9
1-chloro-2,4-dinitrobenzene
-
at pH 7.5 and 30°C
16.4
glutathione
-
at pH 7.5 and 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
55
1-chloro-2,4-dinitrobenzene
-
at pH 7.5 and 30°C
91
glutathione
-
at pH 7.5 and 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000411
-
substrate butachlor
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
assay at
7 - 7.5
-
at 30°C
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
more than 50% activity between pH 6.0 and 8.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 40
-
more than 50% activity between 20 and 40°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
-
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GSTA2; cv. MV Emese, drought-tolerant cv. Plainsman, and drought-sensitive cv. GKE
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
GSTU1B and GSTF6 genes may play important roles in monocarpic senescence of wheat
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GSTF2_WHEAT
291
0
32580
Swiss-Prot
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24500
-
2 * 24500, SDS-PAGE
49500
-
native PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 24490, calculated from amino acid sequence
dimer
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme partially from shoots by ammonium sulfate fractionation and desalting
-
Ni2+-NTA agarose column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
GST genes, DNA and amino acid sequence analysis, phylogenetic tree, clustering of GST coding sequences, expression analysis, overview
expressed in Escherichia coli BL21(DE3) cells
-
GST genes, DNA and amino acid sequence analysis, phylogenetic tree, clustering of GST coding sequences, expression analysis, overview
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
GSTU1B and GSTF6 sequences show high-expression levels that increase with time in well-watered plants and under drought stress in all cultivars, especially in the drought-tolerant Plainsman cultivar
GSTU1B and GSTF6 sequences show high-expression levels that increased with time in well-watered plants and under drought stress in all cultivars, especially in the drought-tolerant Plainsman cultivar
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Edwards, R.
Characterization of glutathione transferases and glutathione peroxidases in pea (Pisum sativum)
Physiol. Plant.
98
594-604
1996
Medicago sativa, Phaseolus coccineus, Phaseolus vulgaris, Pisum sativum, Trifolium repens, Triticum aestivum, Zea mays
-
Manually annotated by BRENDA team
Pascal, S.; Scalla, R.
Purification and characterization of a safener-induced glutathione S-transferase from wheat (Triticum aestivum)
Physiol. Plant.
106
17-27
1999
Triticum aestivum
-
Manually annotated by BRENDA team
Scarponi, L.; Quagliarini, E.; Del Buono, D.
Induction of wheat and maize glutathione S-transferase by some herbicide safeners and their effect on enzyme activity against butachlor and terbuthylazine
Pest Manag. Sci.
62
927-932
2006
Triticum aestivum, Zea mays
Manually annotated by BRENDA team
Galle, A.; Csiszar, J.; Secenji, M.; Guoth, A.; Cseuz, L.; Tari, I.; Gyoergyey, J.; Erdei, L.
Glutathione transferase activity and expression patterns during grain filling in flag leaves of wheat genotypes differing in drought tolerance: response to water deficit
J. Plant Physiol.
166
1878-1891
2009
Triticum aestivum (O04437), Triticum aestivum (P30110), Triticum aestivum (P30111), Triticum aestivum (Q6U6G6), Triticum aestivum (Q8GRI3), Triticum aestivum (Q8GTB7), Triticum aestivum (Q8GTB8), Triticum aestivum (Q8GTB9), Triticum aestivum (Q8GTC0), Triticum aestivum (Q8GTC1), Triticum aestivum (Q8LGN5), Triticum aestivum (Q8LGN6), Triticum aestivum (Q8LL15), Triticum aestivum (Q8RW00), Triticum aestivum (Q8RW01), Triticum aestivum (Q8RW02), Triticum aestivum (Q8RW03), Triticum aestivum (Q8RW04), Triticum aestivum (Q9SP56), Triticum aestivum
Manually annotated by BRENDA team
Yang, X.Q.; Zhang, Y.L.
Characterization of glutathione S-transferases from Sus scrofa, Cydia pomonella and Triticum aestivum: their responses to cantharidin
Enzyme Microb. Technol.
69
1-9
2015
Sus scrofa, Triticum aestivum, Cydia pomonella
Manually annotated by BRENDA team